Status
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Gene information
General information
TC003325 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1245577..1252332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003325
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06889

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003325 RA
      MSSSTSTTS TTLSSVASL VTNSAGADH VHSAPSEPI 
      FLQTKLAQG IAGVFVWAA LLVTCTQIY QHLRWYTNP 
      TEQRWIVRI LFIVPIYAT YSWISLLFF NSESYYVYF 
      FTVRDCYEA FVIYNFLSL CYEYLGGEG NIMSEIRGK 
      PIRSSCLYG TCCLNGKTY TIGFLRFCK QATLQFCLV 
      KPVMAFVII ILQGMGHYR DGDWSPDGG YIYITIIYN 
      ISVSLALYG LFLFYFATR DLLTPFEPV LKFCTVKSV 
      IFLSFWQGV GLAILEKAN VISPIIDSN GTRTSAGTV 
      SAGYQNFLI CIEMFCAAV ALRYAFPYR VYAQGCVTD 
      SRGRSVTMQ SISSSLKET MNPKDIMTD AIHNFHPQY 
      QQYTQYSSE VISHQPYER L
    CDS sequence
    • >TC003325 RA
      atgtcatcc tcaacttct actactagc accacgtta 
      tcttcagta gctagttta gtgacaaat tctgcagga 
      gccgatcat gtccattcg gcaccttca gaacctatc 
      tttcttcag actaaatta gctcagggc attgccgga 
      gtatttgta tgggctgct ctgttggtt acttgtaca 
      caaatttat cagcatttg cgatggtac actaatcca 
      acggaacag cgatggata gtgagaata ttatttata 
      gtacctatc tacgcgacg tattcatgg atcagtctt 
      ttgttcttc aactcggag agctattac gtctacttt 
      ttcaccgtg agagactgt tacgaagct ttcgtaata 
      tacaatttc ctgtcgctt tgctatgaa tacttggga 
      ggtgaaggc aacatcatg tcggaaatt cggggcaag 
      cctattcgc tccagttgc ctttacgga acctgttgt 
      ttgaacggc aaaacatac acgatcggt tttctccga 
      ttttgcaaa caagccact ctgcaattt tgcttagta 
      aaaccagta atggctttc gttataata atcctacaa 
      ggcatggga cattatcgc gatggcgat tggagtcca 
      gacggcggt tatatttac attacgata atctacaac 
      atttccgtg tctcttgcc ctctacgga ctgtttttg 
      ttttatttt gccactaga gaccttctc acgccgttt 
      gagcccgtt ctgaagttt tgtacagtc aagtctgtg 
      attttcttg tcgttttgg caaggggtt ggtttagcc 
      attttggaa aaagccaat gtgatctct ccgattatt 
      gatagtaac ggtactcgg acgtcagcc ggtactgtt 
      tcagccggt tatcagaat ttcttgatc tgcattgaa 
      atgttttgt gcggctgtt gctttaagg tacgctttt 
      ccctatcgg gtgtatgcc caaggatgc gttactgat 
      tcgcgtggt cgatcagtc acgatgcaa agtatttcc 
      agtagccta aaagaaaca atgaatccc aaggacatt 
      atgaccgat gccattcat aatttccat ccacagtat 
      caacaatac acacagtat agctcagag gtcatctca 
      caccaacca tatgagaga ctgtga
    mRNA sequence
    • >TC003325 RA
      taagaaaaa ataatttaa aattttcat tttccttct 
      ttgtattga cttgactgt cgcaacgcc tgcgcaaaa 
      aaagaggtc atcgcatcg caaaatatg gcggaatct 
      gttgtgtgc agtgtgttg gtgatttgt gatttgaag 
      caaattgcc ctttatagg gcttagatt aataacaca 
      atgtttgtg ttgttggtg ttatagtgt atttgaacg 
      taaatcaaa cagacactg ttggaaatc ttcagaatt 
      tgtgagaca tcaagattt tcttttttg acagtttgg 
      aaattcgta cagtatgtc atcctcaac ttctactac 
      tagcaccac gttatcttc agtagctag tttagtgac 
      aaattctgc aggagccga tcatgtcca ttcggcacc 
      ttcagaacc tatctttct tcagactaa attagctca 
      gggcattgc cggagtatt tgtatgggc tgctctgtt 
      ggttacttg tacacaaat ttatcagca tttgcgatg 
      gtacactaa tccaacgga acagcgatg gatagtgag 
      aatattatt tatagtacc tatctacgc gacgtattc 
      atggatcag tcttttgtt cttcaactc ggagagcta 
      ttacgtcta ctttttcac cgtgagaga ctgttacga 
      agctttcgt aatatacaa tttcctgtc gctttgcta 
      tgaatactt gggaggtga aggcaacat catgtcgga 
      aattcgggg caagcctat tcgctccag ttgccttta 
      cggaacctg ttgtttgaa cggcaaaac atacacgat 
      cggttttct ccgattttg caaacaagc cactctgca 
      attttgctt agtaaaacc agtaatggc tttcgttat 
      aataatcct acaaggcat gggacatta tcgcgatgg 
      cgattggag tccagacgg cggttatat ttacattac 
      gataatcta caacatttc cgtgtctct tgccctcta 
      cggactgtt tttgtttta ttttgccac tagagacct 
      tctcacgcc gtttgagcc cgttctgaa gttttgtac 
      agtcaagtc tgtgatttt cttgtcgtt ttggcaagg 
      ggttggttt agccatttt ggaaaaagc caatgtgat 
      ctctccgat tattgatag taacggtac tcggacgtc 
      agccggtac tgtttcagc cggttatca gaatttctt 
      gatctgcat tgaaatgtt ttgtgcggc tgttgcttt 
      aaggtacgc ttttcccta tcgggtgta tgcccaagg 
      atgcgttac tgattcgcg tggtcgatc agtcacgat 
      gcaaagtat ttccagtag cctaaaaga aacaatgaa 
      tcccaagga cattatgac cgatgccat tcataattt 
      ccatccaca gtatcaaca atacacaca gtatagctc 
      agaggtcat ctcacacca accatatga gagactgtg 
      acgttgcta gcatgccgc tgcatgact gcctctggt 
      gctagctag ctatatcat ctcactgat gtaccccac 
      atacactgt ttcatactc tcagggttc ttcagataa 
      aggatttta tcgttaatg cctttaatg acgtaattc 
      ctgtactgt aactgcaca acactgtac attttccta 
      ctataaaaa aattaatag agtgcaaaa ttaagtacg 
      aacgaacaa ggaattgta tgttacgtt gtgatattt 
      ttatttaga actacgtat tcgcagtaa gttgaaagt 
      tattatctt agtggggtg agcaacttc actcaacgt 
      tttgtacat actgtgagt gaagagttt taaagacac 
      accatcgtt gaattattt tttagttgt tgttgatct 
      tgaggctta ccgttaccc catttcgcg ttaagtttc 
      ctaaccttg tatttacgt ccgtctaat tcaagtact 
      taagcatta aaagtgtat atatgtatt gtattttta 
      ttgatttac tttccctag ttgtagtaa tttaatcat 
      tgtacgtat tatttttta tcaaataaa tggtttggt 
      gtgcatccg tttcgttgt tccgttggc atgcttcgt 
      gaccagtga tgacatttg ggcttttca gggggaaaa 
      agtggtcgt ggtatgagg ttatctact tacgaccca 
      gacgatccc atgagtcct agtggagga acggtgcct 
      cagtgctac aacagttta gataacccc tcgagacca 
      cccataacg gcaggaccg cgcgtttcc acaatatcg 
      caaaattac accgaaaaa acgatgttg ttgagttcg 
      gacgaagac aattgagga tttgtttag tcaatttct 
      agcgaattt ctttttatt tttatgtat tacaaagaa 
      agtattttt ctataatag attagctat tatgaccat 
      tcctttaac gtcatgaga tgttcttgt tattacatg 
      tacttaact tatattgca tataatcga agtaatcat 
      acgtgtgat aagcatttt ctccattcc acaggtgtc 
      tctctattg tcagttccc aacaaaata cagtgaaac 
      ccatttgct aatttcatt gtttatgtg gaaactgaa 
      ctaaatgta attgttgcg ttttggctg tgacgcaga 
      cgtgttatc taaaggccg gcttcaagt tattttaaa 
      tcaattaaa aatcatcta tttgaaaac aactaagag 
      acaaaacaa gaaaatttt ctacagaaa agtagattc 
      gaattaggg tcatcctgg accgatctg ctgaaactt 
      agttgatca aatacaggg tgtaacgaa aagatggta 
      tgatgataa accttagat tccccagaa attaaaact 
      tattttgcg ttttctaag aaacaaggg attttgtaa 
      ggtgttttt ttatgcttt gattttgaa tgattttca 
      aagttttcc tccaaagtg cccaatttt tgtagtcca 
      tagattttt aaatatttt tggggcaca caaacggcc 
      gtaaatcac tgcggaaaa tggctaaaa tttaatttt 
      ttttagtcc ttttaatca tggttttga acatatttt 
      tttaggcaa cccattgaa gaaaaatca aaagctgaa 
      tgtttcttc tctcaaaac caaagtctt tatgtatac 
      tatttttat ttggttacc tttttattg ttttattga 
      aattgttaa atgtttatt tatgaataa aaatatact 
      gttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY