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Gene information
General information
TC032308
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003318
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1192377..1198961
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032308
    iBeetle screen iB_06886

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032308 RA
      MAGRNRPVQ HKNFCTIFT KLQGAFSEA VTHQKYVTD 
      KRTLEKTSK LMDKVVKLC QHQKMNLKN SPPFILDIL 
      PDTYQRLRF ICSKYEDNM IALHNNEHF HIFIINLMR 
      KCKQAIKLF KEGKEKMFD ENSHYRRNL TKLSLVFSH 
      MLSELKALF PNGVFAGDQ FRITKADAA EFWKTNFGN 
      STLVPWKLF RQELNNVHP ISSGLEAMA LKSTIDLTC 
      NDYISNFEF DVFTRLFQP WATLLRNWQ ILAVTHPGY 
      VAFLTYDEV KLRLQRYIH KAGSYVFRL SCTRLGQWA 
      IGYVTADGE ILQTIPQNK SLIQALLDG HREGFYLFP 
      DGRSVNPDL SWAVQPTPE DHIAVTQEQ YELYCEMGS 
      TFQLCKICA ENDKDVRIE PCGHLLCTP CLTAWQIDS 
      DGQGCPFCR GRDQRHRTD SCRPFRP
    CDS sequence
    • >TC032308 RA
      atggcaggc cgaaatcgt ccagtccaa cacaaaaac 
      ttttgtaca atatttacc aagttacag ggtgctttt 
      tcggaggca gtgacccac cagaagtat gtgacggac 
      aaacggact ttggaaaaa acgtccaaa ctcatggac 
      aaagtagtg aaactgtgc caacatcaa aaaatgaac 
      ttgaagaac tcgccgcct tttatacta gatatttta 
      ccagacact tatcaaaga ctgcggttc atttgctcc 
      aagtacgaa gacaatatg attgctttg cacaataac 
      gaacatttt cacatattc atcatcaac ctgatgcgg 
      aagtgtaaa caagcaatc aaattgttc aaagagggc 
      aaggagaag atgttcgac gaaaactcg cactacaga 
      aggaactta accaagctc agtttagtg tttagtcat 
      atgttaagt gaactgaaa gccttattc ccgaacggg 
      gtgtttgcc ggagaccag ttccggatc accaaagcg 
      gatgcggcc gaattctgg aagaccaac tttggtaac 
      agcaccttg gtaccgtgg aaacttttt cgtcaggaa 
      ctaaacaac gtccatccg atcagctcc ggcctagaa 
      gccatggct ttgaaatcc accatagac ctcacttgt 
      aacgattac atatcaaat tttgagttt gatgtattc 
      acgagattg ttccaaccc tgggcgacg ctgttacga 
      aactggcaa atattggcg gtcactcat ccgggttat 
      gttgcattc ctgacttac gacgaagtc aaattaaga 
      ctgcagcgg tacattcac aaagctgga agctacgta 
      tttagactc tcttgcacg cgactgggt caatgggcc 
      atcggctac gtcacagcg gacggagaa atcctccaa 
      acgataccc caaaataaa agtctgatc caggcacta 
      ctcgacgga caccgcgaa ggattctat ttgtttccc 
      gacggccga tcggtgaat ccggacttg tcgtgggcg 
      gtgcaaccc acccccgaa gaccacatt gccgttact 
      caggaacaa tacgaactg tactgcgag atgggaagc 
      acattccag ctgtgcaaa atctgcgct gaaaacgac 
      aaggatgtc aggattgag ccctgcgga catttgctg 
      tgcaccccc tgcctgact gcctggcag atcgattcc 
      gacggccag ggctgcccg ttctgcagg ggccgagat 
      caaaggcac cgaacagat agttgtaga ccctttcga 
      ccctaa
    mRNA sequence
    • >TC032308 RA
      ctgaattcg aaattgccg ctgcacatc aaaatgtgc 
      ttagctaca atttgctgg aacagcaac tgaataatg 
      aaagtgtca gttggtggc attgtacat aatcgtgaa 
      gttagctac ttagaattt atcagagtc gggtttata 
      atatttgta atttcacat tattgcctg tttttgctt 
      taatttgga tctgatatt ttgatttgt gtagtcgct 
      caaacgcgc caaaaacct ctacaagcg atttaacct 
      taaaaaagt ctgtttctg tgatgtggt tttattaca 
      tcgcttgag gttattgcg gaatgttgg aagattaat 
      ggaatttct gaatttgag agtggaaaa ggcgtaaaa 
      aatgtttgt gaaaagtac cgttattta caaggcagc 
      cggtcctgg atgcatcgt cggcaactt cacgtcggt 
      atgcaataa gaatgtcag tgatgtgtg tcgacagta 
      gttgtgaat gtttactcc cgaagataa ttaatcaag 
      tgttttaaa ttaaaaatg attaagaaa atgccagtg 
      aaaaaagct tgtgcgatc gtgcgttaa agtttgaga 
      tgtgaggtc atcgccaca gttcaaaat ggcaggccg 
      aaatcgtcc agtccaaca caaaaactt ttgtacaat 
      atttaccaa gttacaggg tgctttttc ggaggcagt 
      gacccacca gaagtatgt gacggacaa acggacttt 
      ggaaaaaac gtccaaact catggacaa agtagtgaa 
      actgtgcca acatcaaaa aatgaactt gaagaactc 
      gccgccttt tatactaga tattttacc agacactta 
      tcaaagact gcggttcat ttgctccaa gtacgaaga 
      caatatgat tgctttgca caataacga acattttca 
      catattcat catcaacct gatgcggaa gtgtaaaca 
      agcaatcaa attgttcaa agagggcaa ggagaagat 
      gttcgacga aaactcgca ctacagaag gaacttaac 
      caagctcag tttagtgtt tagtcatat gttaagtga 
      actgaaagc cttattccc gaacggggt gtttgccgg 
      agaccagtt ccggatcac caaagcgga tgcggccga 
      attctggaa gaccaactt tggtaacag caccttggt 
      accgtggaa actttttcg tcaggaact aaacaacgt 
      ccatccgat cagctccgg cctagaagc catggcttt 
      gaaatccac catagacct cacttgtaa cgattacat 
      atcaaattt tgagtttga tgtattcac gagattgtt 
      ccaaccctg ggcgacgct gttacgaaa ctggcaaat 
      attggcggt cactcatcc gggttatgt tgcattcct 
      gacttacga cgaagtcaa attaagact gcagcggta 
      cattcacaa agctggaag ctacgtatt tagactctc 
      ttgcacgcg actgggtca atgggccat cggctacgt 
      cacagcgga cggagaaat cctccaaac gatacccca 
      aaataaaag tctgatcca ggcactact cgacggaca 
      ccgcgaagg attctattt gtttcccga cggccgatc 
      ggtgaatcc ggacttgtc gtgggcggt gcaacccac 
      ccccgaaga ccacattgc cgttactca ggaacaata 
      cgaactgta ctgcgagat gggaagcac attccagct 
      gtgcaaaat ctgcgctga aaacgacaa ggatgtcag 
      gattgagcc ctgcggaca tttgctgtg caccccctg 
      cctgactgc ctggcagat cgattccga cggccaggg 
      ctgcccgtt ctgcagggg ccgagatca aaggcaccg 
      aacagatag ttgtagacc ctttcgacc ctaaaaaat 
      cccataaca gacccaaaa catcgtcgg ctagctctc 
      cgaacgcgg gcaacatcg acgacgacg atatggagt 
      ttgaggtag tgaacgagc ctcctagca cgaatgatt 
      atgttgaat gagcgaatc cctttctat tctggctgt 
      gcttcaaaa cagaatctt gtttttcgt gtgtgcttg 
      ttttaagct ttatttaaa cactgggtt tgtcttgtc 
      tttcgagat taccgtcgg gtcttgtgc atgtcactt 
      aaagctctg gtgagaaaa agttgtgtt tttctaaat 
      tattctctt ataattatg ttaattttt attttacgc 
      atacatgta cccttgcat atctctacg gaaatactg 
      ggtaatcaa gaggtggtg tttttattt gatttttgt 
      acttagtac caaacgagt attattatc tagggagtc 
      tttatgtac ttttccaaa atttggcga agaaattat 
      ttttgttac tttaagaat ttgtgtaac aatatacaa 
      ttactaata ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY