| General information | |||
| TC003293 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 821683..829226 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003293 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06877 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003293 RA
MSKPRNPKI HNVRFYKPK PNAVYCMAL QPQGNKLAV SRADASVEI WNLTHCAFI ERTIASSTE NFSIEGLAW CDDRLFSVG LHGLLIEYD LMKLDLKSR SVVTGEAAF CLDVNKEKT QIAIGTEQG YLNIFQITE DEVLFEKFL DKQEGRILC LKFDNSGEF IVSGSMDAI RIWSVKTNQ ALHKMIPGR AEHNKPTIV WCLAITQDF TVISGDSRG ILTVWDGKV GAQLESYQS HRADILSLC LSEDENSLY CAGIDPNVV NYVRIEVKD GAHKWVRSI QRKIHDHDV RALVLNGNK LYSSGVDGY LTCSYHPPK TLLKYPPIL QNPCVTICA KGRYVMLRY PKLIEIWSL GEIGSDRDK HTGLYNVEK APRKLVALQ KVVTDDEGV EKHEGIICS TISDDGTLI LYSTRLGVR LLKLDCGES TPKLVPIET DGLGVSPCV SAKFTKNNR LILAPNSGG LQVVAISSC NAVLMQNIN TETEIEDMI VLLAVSDSA QFLVGGDVR GNIAVWTLK NDQYVFHCK LPKYKLSPT AIAIQPNSA NLVVAFNNN NIIEYDMNR RELTKFSRS LAKNLPKQL TSRVSPIRN ITFDSRVKD VIILHDDDS VLVVDKEKT TTNRESKVR KIDGESKEQ FAVRCIKKF KHLVHLSWI SDDEMVAVE VNPLSLIEQ LPPCLVQNT FGRK
- >TC003293 RA
atgagtaaa ccgaggaac ccgaagata cacaatgtt aggttttac aaacctaaa ccgaatgca gtttactgt atggctctc caaccccag ggcaataag ttagctgtg tcaagggct gacgcaagt gtcgaaata tggaacttg actcactgt gcctttatc gaacgtaca attgcttcc tcgactgag aatttcagt attgaaggt ctcgcatgg tgcgatgat cgcttattt agtgtgggg cttcatggg cttttaatt gagtacgat ttaatgaaa ttggatctg aaaagtcgg tctgtggtc accggcgag gcagcgttt tgtctggat gttaataaa gaaaaaact caaattgca attgggact gaacaaggc tatttgaac atttttcaa attaccgag gatgaggtc ctgtttgaa aagttttta gataagcag gaagggcgc attttatgt ctaaagttt gataattca ggcgaattt atcgtttct ggaagtatg gatgcgatc cgaatttgg agcgtcaaa accaatcaa gcgttgcat aaaatgatc cctggtcgt gctgaacac aacaaacca acaatagtg tggtgtctt gccatcaca caagacttt acagtaatc agtggcgat tcccggggc attctcacc gtctgggat ggcaaagtg ggcgctcaa cttgaatcg taccaatca catcgtgca gatattctt tcactttgc ctgtcagag gacgaaaac tcactttat tgcgctggt atagaccca aatgtggtc aattatgtg cgaattgaa gtcaaagac ggagctcat aaatgggtg cgtagcatc caaagaaaa attcacgac cacgatgtg cgcgctctt gttctcaat ggcaataaa ttgtactcg agcggagtc gatggctac ttgacttgt agttatcat ccgccgaaa actctcctt aagtatcca ccaattttg caaaaccct tgtgtgact atttgtgca aaagggcga tacgtgatg ttacgttac cccaaatta atcgaaatt tggagtctt ggagagatt gggagcgat cgtgataaa cacacggga ctctacaat gtggagaaa gctcctcgg aaattggtg gcgttgcaa aaagttgtc actgacgat gagggagtt gaaaaacac gaagggatt atttgtagt acgatttcg gacgatggt actttaatt ttgtacagt acaagacta ggagttagg ttacttaag ttagattgt ggggaaagt accccaaaa ttggtgccg attgagaca gacgggtta ggggtgtct ccatgcgtt agtgcaaaa tttacgaaa aataatcgc ttgattttg gcgccaaat tcggggggt ttacaggtt gttgcaata agtagttgc aacgccgtc ttgatgcaa aatatcaat acagaaaca gaaattgag gatatgatt gtgttattg gcggtctct gactctgcc cagtttttg gtaggtggt gatgttaga ggtaatatt gctgtgtgg actttaaaa aatgaccaa tatgttttt cactgtaaa ttacccaaa tataaatta agtcccact gcgatagcc attcaaccg aattcagcg aatctagta gttgcgttt aataataat aacattatt gaatatgat atgaatagg agagaatta acgaaattt tccagatct ttggcgaaa aatcttccg aaacaattg acgtctaga gtttcccca attcgcaat attactttt gattctagg gtaaaagat gttattatt ttacatgat gatgatagc gttttggtc gtcgacaaa gaaaagact acaaccaat agggaatcc aaggtgcga aaaatcgac ggcgaaagc aaagaacag tttgcagtt cgttgtata aaaaaattc aaacacctg gtgcacctg agctggatt tccgatgat gaaatggtg gcagtggag gtcaatcct ttgagtctc atcgagcaa ctgccccca tgtctggtg caaaatact tttggccga aaataa
- >TC003293 RA
tacttacac ttttataac aatagacat tttttattt gggtcacaa taacaaaaa aattgtaaa aacttcgcg cataaaagt gagggcggc accaccaac cggtcgtaa ccgaagtta ctagaataa taaacaaca cgtgaaagt gaagtttga ggttatgtt tttcatttg tgagtgttt tatttattg aaaatgagt aaaccgagg aacccgaag atacacaat gttaggttt tacaaacct aaaccgaat gcagtttac tgtatggct ctccaaccc cagggcaat aagttagct gtgtcaagg gctgacgca agtgtcgaa atatggaac ttgactcac tgtgccttt atcgaacgt acaattgct tcctcgact gagaatttc agtattgaa ggtctcgca tggtgcgat gatcgctta tttagtgtg gggcttcat gggctttta attgagtac gatttaatg aaattggat ctgaaaagt cggtctgtg gtcaccggc gaggcagcg ttttgtctg gatgttaat aaagaaaaa actcaaatt gcaattggg actgaacaa ggctatttg aacattttt caaattacc gaggatgag gtcctgttt gaaaagttt ttagataag caggaaggg cgcatttta tgtctaaag tttgataat tcaggcgaa tttatcgtt tctggaagt atggatgcg atccgaatt tggagcgtc aaaaccaat caagcgttg cataaaatg atccctggt cgtgctgaa cacaacaaa ccaacaata gtgtggtgt cttgccatc acacaagac tttacagta atcagtggc gattcccgg ggcattctc accgtctgg gatggcaaa gtgggcgct caacttgaa tcgtaccaa tcacatcgt gcagatatt ctttcactt tgcctgtca gaggacgaa aactcactt tattgcgct ggtatagac ccaaatgtg gtcaattat gtgcgaatt gaagtcaaa gacggagct cataaatgg gtgcgtagc atccaaaga aaaattcac gaccacgat gtgcgcgct cttgttctc aatggcaat aaattgtac tcgagcgga gtcgatggc tacttgact tgtagttat catccgccg aaaactctc cttaagtat ccaccaatt ttgcaaaac ccttgtgtg actatttgt gcaaaaggg cgatacgtg atgttacgt taccccaaa ttaatcgaa atttggagt cttggagag attgggagc gatcgtgat aaacacacg ggactctac aatgtggag aaagctcct cggaaattg gtggcgttg caaaaagtt gtcactgac gatgaggga gttgaaaaa cacgaaggg attatttgt agtacgatt tcggacgat ggtacttta attttgtac agtacaaga ctaggagtt aggttactt aagttagat tgtggggaa agtacccca aaattggtg ccgattgag acagacggg ttaggggtg tctccatgc gttagtgca aaatttacg aaaaataat cgcttgatt ttggcgcca aattcgggg ggtttacag gttgttgca ataagtagt tgcaacgcc gtcttgatg caaaatatc aatacagaa acagaaatt gaggatatg attgtgtta ttggcggtc tctgactct gcccagttt ttggtaggt ggtgatgtt agaggtaat attgctgtg tggacttta aaaaatgac caatatgtt tttcactgt aaattaccc aaatataaa ttaagtccc actgcgata gccattcaa ccgaattca gcgaatcta gtagttgcg tttaataat aataacatt attgaatat gatatgaat aggagagaa ttaacgaaa ttttccaga tctttggcg aaaaatctt ccgaaacaa ttgacgtct agagtttcc ccaattcgc aatattact tttgattct agggtaaaa gatgttatt attttacat gatgatgat agcgttttg gtcgtcgac aaagaaaag actacaacc aatagggaa tccaaggtg cgaaaaatc gacggcgaa agcaaagaa cagtttgca gttcgttgt ataaaaaaa ttcaaacac ctggtgcac ctgagctgg atttccgat gatgaaatg gtggcagtg gaggtcaat cctttgagt ctcatcgag caactgccc ccatgtctg gtgcaaaat acttttggc cgaaaataa atgttttaa taaataacg tgtgttttt
