Status
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Gene information
General information
TC003293 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 821683..829226
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003293
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06877

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003293 RA
      MSKPRNPKI HNVRFYKPK PNAVYCMAL QPQGNKLAV 
      SRADASVEI WNLTHCAFI ERTIASSTE NFSIEGLAW 
      CDDRLFSVG LHGLLIEYD LMKLDLKSR SVVTGEAAF 
      CLDVNKEKT QIAIGTEQG YLNIFQITE DEVLFEKFL 
      DKQEGRILC LKFDNSGEF IVSGSMDAI RIWSVKTNQ 
      ALHKMIPGR AEHNKPTIV WCLAITQDF TVISGDSRG 
      ILTVWDGKV GAQLESYQS HRADILSLC LSEDENSLY 
      CAGIDPNVV NYVRIEVKD GAHKWVRSI QRKIHDHDV 
      RALVLNGNK LYSSGVDGY LTCSYHPPK TLLKYPPIL 
      QNPCVTICA KGRYVMLRY PKLIEIWSL GEIGSDRDK 
      HTGLYNVEK APRKLVALQ KVVTDDEGV EKHEGIICS 
      TISDDGTLI LYSTRLGVR LLKLDCGES TPKLVPIET 
      DGLGVSPCV SAKFTKNNR LILAPNSGG LQVVAISSC 
      NAVLMQNIN TETEIEDMI VLLAVSDSA QFLVGGDVR 
      GNIAVWTLK NDQYVFHCK LPKYKLSPT AIAIQPNSA 
      NLVVAFNNN NIIEYDMNR RELTKFSRS LAKNLPKQL 
      TSRVSPIRN ITFDSRVKD VIILHDDDS VLVVDKEKT 
      TTNRESKVR KIDGESKEQ FAVRCIKKF KHLVHLSWI 
      SDDEMVAVE VNPLSLIEQ LPPCLVQNT FGRK
    CDS sequence
    • >TC003293 RA
      atgagtaaa ccgaggaac ccgaagata cacaatgtt 
      aggttttac aaacctaaa ccgaatgca gtttactgt 
      atggctctc caaccccag ggcaataag ttagctgtg 
      tcaagggct gacgcaagt gtcgaaata tggaacttg 
      actcactgt gcctttatc gaacgtaca attgcttcc 
      tcgactgag aatttcagt attgaaggt ctcgcatgg 
      tgcgatgat cgcttattt agtgtgggg cttcatggg 
      cttttaatt gagtacgat ttaatgaaa ttggatctg 
      aaaagtcgg tctgtggtc accggcgag gcagcgttt 
      tgtctggat gttaataaa gaaaaaact caaattgca 
      attgggact gaacaaggc tatttgaac atttttcaa 
      attaccgag gatgaggtc ctgtttgaa aagttttta 
      gataagcag gaagggcgc attttatgt ctaaagttt 
      gataattca ggcgaattt atcgtttct ggaagtatg 
      gatgcgatc cgaatttgg agcgtcaaa accaatcaa 
      gcgttgcat aaaatgatc cctggtcgt gctgaacac 
      aacaaacca acaatagtg tggtgtctt gccatcaca 
      caagacttt acagtaatc agtggcgat tcccggggc 
      attctcacc gtctgggat ggcaaagtg ggcgctcaa 
      cttgaatcg taccaatca catcgtgca gatattctt 
      tcactttgc ctgtcagag gacgaaaac tcactttat 
      tgcgctggt atagaccca aatgtggtc aattatgtg 
      cgaattgaa gtcaaagac ggagctcat aaatgggtg 
      cgtagcatc caaagaaaa attcacgac cacgatgtg 
      cgcgctctt gttctcaat ggcaataaa ttgtactcg 
      agcggagtc gatggctac ttgacttgt agttatcat 
      ccgccgaaa actctcctt aagtatcca ccaattttg 
      caaaaccct tgtgtgact atttgtgca aaagggcga 
      tacgtgatg ttacgttac cccaaatta atcgaaatt 
      tggagtctt ggagagatt gggagcgat cgtgataaa 
      cacacggga ctctacaat gtggagaaa gctcctcgg 
      aaattggtg gcgttgcaa aaagttgtc actgacgat 
      gagggagtt gaaaaacac gaagggatt atttgtagt 
      acgatttcg gacgatggt actttaatt ttgtacagt 
      acaagacta ggagttagg ttacttaag ttagattgt 
      ggggaaagt accccaaaa ttggtgccg attgagaca 
      gacgggtta ggggtgtct ccatgcgtt agtgcaaaa 
      tttacgaaa aataatcgc ttgattttg gcgccaaat 
      tcggggggt ttacaggtt gttgcaata agtagttgc 
      aacgccgtc ttgatgcaa aatatcaat acagaaaca 
      gaaattgag gatatgatt gtgttattg gcggtctct 
      gactctgcc cagtttttg gtaggtggt gatgttaga 
      ggtaatatt gctgtgtgg actttaaaa aatgaccaa 
      tatgttttt cactgtaaa ttacccaaa tataaatta 
      agtcccact gcgatagcc attcaaccg aattcagcg 
      aatctagta gttgcgttt aataataat aacattatt 
      gaatatgat atgaatagg agagaatta acgaaattt 
      tccagatct ttggcgaaa aatcttccg aaacaattg 
      acgtctaga gtttcccca attcgcaat attactttt 
      gattctagg gtaaaagat gttattatt ttacatgat 
      gatgatagc gttttggtc gtcgacaaa gaaaagact 
      acaaccaat agggaatcc aaggtgcga aaaatcgac 
      ggcgaaagc aaagaacag tttgcagtt cgttgtata 
      aaaaaattc aaacacctg gtgcacctg agctggatt 
      tccgatgat gaaatggtg gcagtggag gtcaatcct 
      ttgagtctc atcgagcaa ctgccccca tgtctggtg 
      caaaatact tttggccga aaataa
    mRNA sequence
    • >TC003293 RA
      tacttacac ttttataac aatagacat tttttattt 
      gggtcacaa taacaaaaa aattgtaaa aacttcgcg 
      cataaaagt gagggcggc accaccaac cggtcgtaa 
      ccgaagtta ctagaataa taaacaaca cgtgaaagt 
      gaagtttga ggttatgtt tttcatttg tgagtgttt 
      tatttattg aaaatgagt aaaccgagg aacccgaag 
      atacacaat gttaggttt tacaaacct aaaccgaat 
      gcagtttac tgtatggct ctccaaccc cagggcaat 
      aagttagct gtgtcaagg gctgacgca agtgtcgaa 
      atatggaac ttgactcac tgtgccttt atcgaacgt 
      acaattgct tcctcgact gagaatttc agtattgaa 
      ggtctcgca tggtgcgat gatcgctta tttagtgtg 
      gggcttcat gggctttta attgagtac gatttaatg 
      aaattggat ctgaaaagt cggtctgtg gtcaccggc 
      gaggcagcg ttttgtctg gatgttaat aaagaaaaa 
      actcaaatt gcaattggg actgaacaa ggctatttg 
      aacattttt caaattacc gaggatgag gtcctgttt 
      gaaaagttt ttagataag caggaaggg cgcatttta 
      tgtctaaag tttgataat tcaggcgaa tttatcgtt 
      tctggaagt atggatgcg atccgaatt tggagcgtc 
      aaaaccaat caagcgttg cataaaatg atccctggt 
      cgtgctgaa cacaacaaa ccaacaata gtgtggtgt 
      cttgccatc acacaagac tttacagta atcagtggc 
      gattcccgg ggcattctc accgtctgg gatggcaaa 
      gtgggcgct caacttgaa tcgtaccaa tcacatcgt 
      gcagatatt ctttcactt tgcctgtca gaggacgaa 
      aactcactt tattgcgct ggtatagac ccaaatgtg 
      gtcaattat gtgcgaatt gaagtcaaa gacggagct 
      cataaatgg gtgcgtagc atccaaaga aaaattcac 
      gaccacgat gtgcgcgct cttgttctc aatggcaat 
      aaattgtac tcgagcgga gtcgatggc tacttgact 
      tgtagttat catccgccg aaaactctc cttaagtat 
      ccaccaatt ttgcaaaac ccttgtgtg actatttgt 
      gcaaaaggg cgatacgtg atgttacgt taccccaaa 
      ttaatcgaa atttggagt cttggagag attgggagc 
      gatcgtgat aaacacacg ggactctac aatgtggag 
      aaagctcct cggaaattg gtggcgttg caaaaagtt 
      gtcactgac gatgaggga gttgaaaaa cacgaaggg 
      attatttgt agtacgatt tcggacgat ggtacttta 
      attttgtac agtacaaga ctaggagtt aggttactt 
      aagttagat tgtggggaa agtacccca aaattggtg 
      ccgattgag acagacggg ttaggggtg tctccatgc 
      gttagtgca aaatttacg aaaaataat cgcttgatt 
      ttggcgcca aattcgggg ggtttacag gttgttgca 
      ataagtagt tgcaacgcc gtcttgatg caaaatatc 
      aatacagaa acagaaatt gaggatatg attgtgtta 
      ttggcggtc tctgactct gcccagttt ttggtaggt 
      ggtgatgtt agaggtaat attgctgtg tggacttta 
      aaaaatgac caatatgtt tttcactgt aaattaccc 
      aaatataaa ttaagtccc actgcgata gccattcaa 
      ccgaattca gcgaatcta gtagttgcg tttaataat 
      aataacatt attgaatat gatatgaat aggagagaa 
      ttaacgaaa ttttccaga tctttggcg aaaaatctt 
      ccgaaacaa ttgacgtct agagtttcc ccaattcgc 
      aatattact tttgattct agggtaaaa gatgttatt 
      attttacat gatgatgat agcgttttg gtcgtcgac 
      aaagaaaag actacaacc aatagggaa tccaaggtg 
      cgaaaaatc gacggcgaa agcaaagaa cagtttgca 
      gttcgttgt ataaaaaaa ttcaaacac ctggtgcac 
      ctgagctgg atttccgat gatgaaatg gtggcagtg 
      gaggtcaat cctttgagt ctcatcgag caactgccc 
      ccatgtctg gtgcaaaat acttttggc cgaaaataa 
      atgttttaa taaataacg tgtgttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY