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Gene information
General information
TC003248 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 319009..320297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003248
    iBeetle screen iB_06869

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003248 RA
      MSESSKRQV PPAMKKLLS LDAEITKRF 
      VLWANQFLP LRSLRIHYK ALEITCHGI 
      PWFAFWIAF TWLFNNPSL VQLQVNMLM 
      GLLIDIILI AVAKAYFRR RRPMANTDD 
      ALGQIGPDV FSFPSGHAS RAVFVAYFF 
      INLWPLPIF CIPPLLAWV TAICLSRIL 
      MNRHYILDV IGGIVFGLV VSWTVCLIW 
      LDKDAASYL MSFLSDDKI DGGDYHV
    CDS sequence
    • >TC003248 RA
      atgtctgag tctagtaaa cggcaagtg cccccagct 
      atgaaaaaa ctcctaagt cttgatgcc gaaataacc 
      aaacgcttt gtcttgtgg gctaatcaa tttttgccc 
      ttgaggtca cttcgtatc cattacaag gctttggaa 
      ataacgtgt catggaatc ccttggttt gcattctgg 
      attgcattt acttggtta tttaataat ccgtctcta 
      gtccagtta caagtcaac atgttgatg ggtctttta 
      attgatata atcctcata gctgtggct aaagcctat 
      ttccggaga cggcggcct atggctaat actgacgac 
      gccttgggt cagataggc cctgatgtg tttagtttc 
      ccgtccggt catgcgagt cgtgccgtt tttgttgcc 
      tattttttc atcaatttg tggcctttg cccattttt 
      tgcatacca cctttacta gcttgggtc acagctatc 
      tgtctgagc agaattttg atgaatcga cactatatt 
      ttagatgtc attggtggg atagttttc ggattggtt 
      gtctcatgg actgtttgt ttaatctgg cttgataaa 
      gacgccgct agttattta atgtccttt ttatcagac 
      gataaaata gacgggggt gactatcat gtgtga
    mRNA sequence
    • >TC003248 RA
      tcgccgcaa tgaaatttc gtgtttatt attagtcac 
      ttaaaaata gataagaag atccacact ctcttattt 
      acttttttt agtaataag cagcatgtc tgagtctag 
      taaacggca agtgccccc agctatgaa aaaactcct 
      aagtcttga tgccgaaat aaccaaacg ctttgtctt 
      gtgggctaa tcaattttt gcccttgag gtcacttcg 
      tatccatta caaggcttt ggaaataac gtgtcatgg 
      aatcccttg gtttgcatt ctggattgc atttacttg 
      gttatttaa taatccgtc tctagtcca gttacaagt 
      caacatgtt gatgggtct tttaattga tataatcct 
      catagctgt ggctaaagc ctatttccg gagacggcg 
      gcctatggc taatactga cgacgcctt gggtcagat 
      aggccctga tgtgtttag tttcccgtc cggtcatgc 
      gagtcgtgc cgtttttgt tgcctattt tttcatcaa 
      tttgtggcc tttgcccat tttttgcat accaccttt 
      actagcttg ggtcacagc tatctgtct gagcagaat 
      tttgatgaa tcgacacta tattttaga tgtcattgg 
      tgggatagt tttcggatt ggttgtctc atggactgt 
      ttgtttaat ctggcttga taaagacgc cgctagtta 
      tttaatgtc ctttttatc agacgataa aatagacgg 
      gggtgacta tcatgtgtg ataataatt aaaaacgaa 
      tttgctatt tatcatttt attaagact tttttacac 
      ggaattaca ttgtagaaa taaggcccg aatttcagg 
      gaatcaaaa agttttcaa taaatatat ttcggtaag 
      cgaggctgt aaataaacg atacgagtt gtaaaatct 
      ttggttgta cataaaaaa cttaagtct agtgttttt 
      gttttactt ttcctgcac ctttctgct ccaggtacc 
      acttagctg cctttcgag tgcgtaatt tagctacat 
      tttaaagct attagcttg agacatgtt acccaacaa 
      gtgcaaaaa ctattcttt tcttcatac aaaaaaatg 
      gaatgtgtt ggggtaact aacaatcta aaaataaat 
      taaaaacat gcccataac aacacctaa tcgtgtgct 
      acgaattga aataaataa ataaaatat atacaataa 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY