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Gene information
General information
TC003235 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 147149..148476
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003235
    iBeetle screen iB_06867

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    tropharium surface irregular - Number of animals showing the phenotype: 1

    vitellogenic egg chamber late structure irregular - Number of animals showing the phenotype: 2

    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 2

    previtellogenic egg chamber potentially number decreased - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003235 RA
      MANNTSGAS HNFKVVLLG EGCVGKTSL 
      VLRYVEDKF NTNHVTTVQ ASFLNKKLN 
      IDGKRINLA IWDTAGQEK FHALGPIYY 
      RSSNGAVLV YDITDEDSF QKVKSWVKE 
      LRKMLGADI CLVIAGNKT DLEKNRNVS 
      LEEAEAYAI KVGAIHFQT SAKLNKGVE 
      EMFLVLTQK MLETVEEKE AQNAPLSRQ 
      NSQRRNVVV VDDDQVPPP PTRQCCSNS
    CDS sequence
    • >TC003235 RA
      atggccaac aacaccagc ggggcttcc cacaacttt 
      aaggttgtc ttgttgggg gagggttgt gtggggaaa 
      accagcttg gtacttagg tatgtggag gataaattc 
      aacacgaat catgtaaca acggtccag gcttcgttt 
      ctcaacaaa aaactaaat attgacggc aaacgcata 
      aatttggcg atttgggac actgcaggt caggagaag 
      ttccacgcc ttaggccct atttattac agatcgtca 
      aacggtgca gtgttggtg tacgatatc actgatgaa 
      gattcgttt cagaaagtc aaaagttgg gtgaaggaa 
      ttgcggaag atgttgggg gctgatata tgtctagtg 
      attgctgga aataagact gatcttgag aagaaccga 
      aacgttagc ttggaagaa gccgaagcc tatgcgatt 
      aaagtggga gcgatacat tttcaaacg tcggcaaaa 
      ttgaataaa ggcgttgag gagatgttt ctcgttctg 
      acgcagaaa atgttagag actgttgaa gagaaagaa 
      gcgcaaaat gcgcccttg tctagacag aattcgcaa 
      cgtcgcaat gttgttgtt gttgacgat gatcaagtt 
      cctccacca cccacaaga caatgttgt agcaattcg 
      taa
    mRNA sequence
    • >TC003235 RA
      ctgggcaca agtcacgtt ttttgcctg agaaacgca 
      aaaacagtt gttgatttg agtacgact tccggtgtt 
      acgtcaagt ttgagctga cgtttcctt ttacaaccg 
      acttgttta cacgtctta aatcgattt gattcttcg 
      cggtgttat tgtggaccg aaaattttg tcaaaaaat 
      aaccatttg caattaacc aagtcctga aaaaatgtg 
      gttttggcc gaaaattga aaaaatttt gaaaccccc 
      ctagcggcg ttttcctaa actaattgc gacaggtac 
      gcaaagtca cgcctacat aaagaacaa tcagctgat 
      tgtgaaaat gtaatcgaa aataattgt tgattgtgg 
      ggaaaagtc taataaatc atttgtgcg taattgtat 
      ttaatgagt cagtgaaat tcgttgact atggccaac 
      aacaccagc ggggcttcc cacaacttt aaggttgtc 
      ttgttgggg gagggttgt gtggggaaa accagcttg 
      gtacttagg tatgtggag gataaattc aacacgaat 
      catgtaaca acggtccag gcttcgttt ctcaacaaa 
      aaactaaat attgacggc aaacgcata aatttggcg 
      atttgggac actgcaggt caggagaag ttccacgcc 
      ttaggccct atttattac agatcgtca aacggtgca 
      gtgttggtg tacgatatc actgatgaa gattcgttt 
      cagaaagtc aaaagttgg gtgaaggaa ttgcggaag 
      atgttgggg gctgatata tgtctagtg attgctgga 
      aataagact gatcttgag aagaaccga aacgttagc 
      ttggaagaa gccgaagcc tatgcgatt aaagtggga 
      gcgatacat tttcaaacg tcggcaaaa ttgaataaa 
      ggcgttgag gagatgttt ctcgttctg acgcagaaa 
      atgttagag actgttgaa gagaaagaa gcgcaaaat 
      gcgcccttg tctagacag aattcgcaa cgtcgcaat 
      gttgttgtt gttgacgat gatcaagtt cctccacca 
      cccacaaga caatgttgt agcaattcg taaatttgt 
      gttgttatt ttttgtata taatttcgt tttaagtca 
      aaaaatgta acgagagag tcaaggttg taatttgga 
      gcttcctcc aaataatgc tttataaga ataaaccca 
      aatatgtac a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY