Status
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Gene information
General information
TC001375 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15168751..15170476
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001375
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06861

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001375 RA
      MVKSKVNDL RALLAKTLG SDIEITDQK TTLLTAPGE 
HYGSIMLAL DVTLKKSGK EQKYNLVAK LIPANEMLR 
VAFDIYVTF KKEVLAYTE TIPYITQLQ RQYQVPEHH 
LFDKLFAKC YGARLCLDT NKNEVDEDA VLIFENLKL 
QGYKTEDRL VGFDLKASK IILRDLARF HAAPIAVKR 
LKFDDFQAK VLPALAKNK GLEQLPPEV TLAFHNSIM 
EGALEVPEL EEFLPRMQK VVDYAASHP YVNRPPPKE 
LWGSMSHSD FWTSNTMIL RNELGEPVS NKIVDLQIM 
TYSSIARDV IFFMFTSII NRVLEENYE ELLRTYYDS 
FVNTLKEFE LDVSEYSWE NFLKELDEV APSEIYHVL 
VMLKPICTE RGKVENSLE DFQDSDWSR KDLLGPSHR 
KKLKDTVLA FVKRNWI
    CDS sequence
    • >TC001375 RA
      atggtgaaa tcgaaagta aacgatttg cgcgctctt 
ttggccaaa accctagga agtgacatc gaaatcacc 
gaccagaaa accacactt ttaacagca cctggtgaa 
cactacgga agcataatg ctggcactg gacgtcaca 
ctcaaaaaa tccggaaag gaacaaaaa tacaatctc 
gtggcgaaa ttaatccca gccaacgaa atgcttcga 
gtggctttc gatatttac gtaacgttc aaaaaagaa 
gtactagct tacaccgaa accatccct tacatcaca 
caattgcaa cgccagtac caagtacca gagcaccat 
ctctttgac aaactcttt gccaagtgt tacggtgcc 
agactttgc ctggacacc aataaaaac gaagtggat 
gaagacgct gttctaatt tttgaaaat ttgaaactc 
caagggtac aaaacggaa gatcgcttg gttggtttt 
gacttgaaa gcttccaag atcattttg agggacttg 
gcccggttt catgccgcc ccaattgca gtgaaacgg 
ctcaaattt gatgatttc caagccaaa gttctccca 
gcacttgcc aaaaacaag ggcttggag cagctaccc 
ccagaggtg actctagca ttccacaac agtattatg 
gaaggtgct ttggaagtg ccagaactg gaggaattt 
ctaccaagg atgcaaaag gtggtggat tatgccgcc 
tcacaccct tatgtcaat cgaccaccc ccgaaagaa 
ttatggggt agtatgtca cactctgat ttttggacc 
agtaatacc atgattttg cgtaacgag cttggagag 
cctgttagt aacaaaatc gtcgatttg cagatcatg 
acgtatagt tccatagca cgtgatgtg atttttttc 
atgtttact agtattatt aaccgggtg ttggaagag 
aactacgag gagttgctt cggacgtat tatgatagt 
tttgtaaac acgttgaag gagtttgag ttggatgtg 
agtgagtac agttgggag aatttcctc aaagagttg 
gatgaagtt gcaccaagt gaaatttac cacgttttg 
gttatgctg aagccgatt tgtaccgaa agagggaag 
gttgagaac agtctggag gacttccaa gacagtgat 
tggagtcgg aaagatttg ttggggcct tcacaccgc 
aagaaactt aaagatacg gtgctagca tttgtcaag 
agaaactgg atctag
    mRNA sequence
    • >TC001375 RA
      caaacacac aacagttga tagtagacc atcgccaat 
gagttaaat tgtttcaat taatgccct ctccatagt 
actcgtcac gtgacagtg ccctcgtgt gtttgaaca 
gtgtctcgt aaatcagta atctcgcgc tgttcaaaa 
tggtgaaat cgaaagtaa acgatttgc gcgctcttt 
tggccaaaa ccctaggaa gtgacatcg aaatcaccg 
accagaaaa ccacacttt taacagcac ctggtgaac 
actacggaa gcataatgc tggcactgg acgtcacac 
tcaaaaaat ccggaaagg aacaaaaat acaatctcg 
tggcgaaat taatcccag ccaacgaaa tgcttcgag 
tggctttcg atatttacg taacgttca aaaaagaag 
tactagctt acaccgaaa ccatccctt acatcacac 
aattgcaac gccagtacc aagtaccag agcaccatc 
tctttgaca aactctttg ccaagtgtt acggtgcca 
gactttgcc tggacacca ataaaaacg aagtggatg 
aagacgctg ttctaattt ttgaaaatt tgaaactcc 
aagggtaca aaacggaag atcgcttgg ttggttttg 
acttgaaag cttccaaga tcattttga gggacttgg 
cccggtttc atgccgccc caattgcag tgaaacggc 
tcaaatttg atgatttcc aagccaaag ttctcccag 
cacttgcca aaaacaagg gcttggagc agctacccc 
cagaggtga ctctagcat tccacaaca gtattatgg 
aaggtgctt tggaagtgc cagaactgg aggaatttc 
taccaagga tgcaaaagg tggtggatt atgccgcct 
cacaccctt atgtcaatc gaccacccc cgaaagaat 
tatggggta gtatgtcac actctgatt tttggacca 
gtaatacca tgattttgc gtaacgagc ttggagagc 
ctgttagta acaaaatcg tcgatttgc agatcatga 
cgtatagtt ccatagcac gtgatgtga tttttttca 
tgtttacta gtattatta accgggtgt tggaagaga 
actacgagg agttgcttc ggacgtatt atgatagtt 
ttgtaaaca cgttgaagg agtttgagt tggatgtga 
gtgagtaca gttgggaga atttcctca aagagttgg 
atgaagttg caccaagtg aaatttacc acgttttgg 
ttatgctga agccgattt gtaccgaaa gagggaagg 
ttgagaaca gtctggagg acttccaag acagtgatt 
ggagtcgga aagatttgt tggggcctt cacaccgca 
agaaactta aagatacgg tgctagcat ttgtcaaga 
gaaactgga tctagggcc aaatgttgt acaatttta 
tgcaataaa aatatttta ttgactttg tttgggcac 
aattttaga ccagaaatc ctatttttc ctgatttct 
tgtcgcatt tctcaagat ttcgtaaag attgtctaa 
aaaacaatt aatcaaacc gaaaactac tattttcga 
acgatttag ccttagatt ttgcttgtt ttaaatcaa 
aagattggg tcaaaaagc cagaaatct tcgaattag 
gttaaaaat tatataact gaaaaaaat aaattaaat 
aattgaatt aattccaaa ataattttt ttttaaaca 
gagtcaaaa attctaata aaattcaac tcatttt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY