Status
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Gene information
General information
TC011151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3841177..3854058
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011151
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06855

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: larvae die after hatching
    larval musculature random rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: probably dying
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     prothorax cuticle potentially posteriordecreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax anterior ventralbent - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax partially anteriorconstricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen ventral potentiallynot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011151 RA
      MAQTPLVLL LLLCVSSAL TEPTRYNSS DLSYLSNGI 
QFPVPASEL HNPAVEEIF QDVSEVRWL AELFDHLKW 
AGKLKVLNE TRCQTDLEL YVEELLNGT SWAAKMYDS 
SGRYSGQFF FGNDYWLGS KTLCEELTN PETNPEIPP 
FPVTFLMAK VRLNINRNL TPVTRQLNV GECLPSSCT 
PADVRLLLA QDRKAGSTL NVVGIRPVP GDYRLLEDI 
KFQIVGGVT LVVSLLIFV ASLVEIIAN KKQKQKVDA 
ELENNNNVD DAKSTKKDQ KTGKKPKQS LFLRVILCF 
SAIDNGKKI LNVEKVSQD SIKCIHGLR FFSIAWIIM 
VHTYLEVFS IGDNKNLRI LTERNFVYQ TVSNATFSV 
DTFFFISGL LVTITYFRT AAKKPAKEE NTCRSAQTN 
FFKFVMLVI YRFFRLTPA YLFVLGVNE VILRYLHSY 
SVFSPAIID HISCSNFWW RNALYINNF YPQTEFCML 
WSWYIANDT QFYLVASVL LLIAVRGEK HLKFAGAAI 
GVFMVSSWI MTFIIAMKY EYVVRVEEP FALFDQLYD 
KPWMRIGPY FVGMISGYF LFRVKCQVK LPLVVVVLG 
WVLSLACLS SLVYGVGKL GLVVPTSAF YASLGHTAW 
GLSLAWITL ACCSGYGGP ISALLKFKF FLPLSRLTY 
CAYLIHPVF MCLSSFVLD GPIHLNNSL VMVIYCGNI 
VASFVSAFA ISLAFEAPI VNLLKIIF
    CDS sequence
    • >TC011151 RA
      atggcccaa acgcccctc gtgctgctg ctgttgttg 
tgtgtgagc agtgcccta accgaaccg acaagatac 
aattcgtcc gatctttcc tacctatca aacggtatt 
cagttcccg gttccggcc tcagagttg cataatcca 
gctgtagag gaaattttt caggacgtg tcggaagtc 
cggtggttg gccgaactg ttcgaccac ctaaaatgg 
gccggaaag ctcaaagtg ctgaacgag acgaggtgt 
cagacagac ctggagctg tatgtcgaa gagttgctg 
aatgggact tcctgggcg gccaaaatg tacgactca 
tctggtcgc tactcgggc cagttcttc ttcggtaac 
gactactgg ctgggttcg aaaaccctc tgcgaagag 
ctaaccaac cctgagacc aacccggag atccctccc 
tttcctgtc accttcctg atggccaaa gttcggctc 
aacatcaac cgaaatcta acaccagtg accagacag 
ttgaatgtc ggcgaatgt ctgccatct tcttgcact 
cctgcagac gtacgattg ctgctggcg caggatcgc 
aaagctggg tcaacactc aacgtggtg ggaattcgg 
ccggttccg ggggattac aggcttctg gaggatatc 
aaatttcaa attgtggga ggtgtcaca ctggtcgtc 
agcctcctc attttcgtc gcatctttg gtcgaaatc 
atcgcaaac aagaagcag aaacagaaa gtggacgcc 
gaacttgaa aacaacaac aatgtggac gatgccaaa 
agcaccaaa aaagatcaa aaaactggc aaaaaaccc 
aaacaaagt ctcttcctt cgagtcatt ctgtgcttt 
tctgcaatc gacaatggg aaaaaaatc ctcaatgtg 
gaaaaagtc tcgcaggat tcgatcaaa tgcatccac 
ggtctgcgt ttcttctcc atcgcttgg atcatcatg 
gtccacact tacctcgaa gttttctca attggtgac 
aataaaaat ttgaggatt ttgactgag cgcaacttc 
gtgtatcaa accgtctct aatgcgact ttttccgtg 
gacaccttc ttcttcatc agcggcttg ctggtcaca 
atcacttac ttccgaacc gcggccaaa aaaccggcc 
aaggaagag aacacgtgc agatcagcc cagaccaat 
tttttcaaa ttcgtgatg ctagtcatt taccgattc 
ttccgcctc acacctgct tatctcttc gtgttgggt 
gtgaatgaa gtgattttg cgctacttg cacagttat 
tcggtcttt tcaccggcg attattgac cacatcagt 
tgtagcaac ttctggtgg aggaatgct ctctatatt 
aacaatttt tacccccaa acggagttt tgtatgctt 
tggtcgtgg tatatcgcc aatgatacg cagttttat 
ctcgtggct agtgtgctt ctcctgatt gcagtgcga 
ggtgagaaa cacttgaag ttcgccggt gccgcaatt 
ggcgtcttc atggtgtcc tcctggatc atgactttc 
atcatagcg atgaaatac gagtacgtt gtaagagtg 
gaagaacca tttgcgctc tttgaccaa ctttacgac 
aagccctgg atgcggata ggcccgtat ttcgtcgga 
atgatctcc gggtacttc ctcttcagg gtgaaatgc 
caagtcaag cttccacta gttgtggta gtactggga 
tgggtacta tccctggct tgtttatcc agtttagtc 
tacggtgtt ggtaaacta ggcctggta gtaccaaca 
tcagcgttt tacgcttct ttggggcac acagcgtgg 
ggcttgtcc ctggcctgg atcacactg gcgtgctgc 
tcgggctac gggggcccg atcagtgct ctcttaaaa 
ttcaaattc tttctacct ctcagtaga ttaacttat 
tgcgcatat ttgatacat cctgtattt atgtgtttg 
agcagcttc gtcttagat ggaccaatt catctcaac 
aacagtctt gtcatggta atatattgt ggcaacatt 
gtggcctca ttcgtgtca gcattcgcc atttcgctc 
gctttcgaa gcaccgatc gtaaatttg ctcaaaatt 
attttttaa
    mRNA sequence
    • >TC011151 RA
      aatttcaaa atcgcctca gtcctaagc cgaatcgaa 
agtcgcgtc agtgtgcac ccagcagtg ataacgtcc 
acacggtcc aaaatgccc acataaccc tctcgcgaa 
ccagactgt gctctagta ccgtgtgtc ccaaaaatg 
gcccaaacg cccctcgtg ctgctgctg ttgttgtgt 
gtgagcagt gccctaacc gaaccgaca agatacaat 
tcgtccgat ctttcctac ctatcaaac ggtattcag 
ttcccggtt ccggcctca gagttgcat aatccagct 
gtagaggaa atttttcag gacgtgtcg gaagtccgg 
tggttggcc gaactgttc gaccaccta aaatgggcc 
ggaaagctc aaagtgctg aacgagacg aggtgtcag 
acagacctg gagctgtat gtcgaagag ttgctgaat 
gggacttcc tgggcggcc aaaatgtac gactcatct 
ggtcgctac tcgggccag ttcttcttc ggtaacgac 
tactggctg ggttcgaaa accctctgc gaagagcta 
accaaccct gagaccaac ccggagatc cctcccttt 
cctgtcacc ttcctgatg gccaaagtt cggctcaac 
atcaaccga aatctaaca ccagtgacc agacagttg 
aatgtcggc gaatgtctg ccatcttct tgcactcct 
gcagacgta cgattgctg ctggcgcag gatcgcaaa 
gctgggtca acactcaac gtggtggga attcggccg 
gttccgggg gattacagg cttctggag gatatcaaa 
tttcaaatt gtgggaggt gtcacactg gtcgtcagc 
ctcctcatt ttcgtcgca tctttggtc gaaatcatc 
gcaaacaag aagcagaaa cagaaagtg gacgccgaa 
cttgaaaac aacaacaat gtggacgat gccaaaagc 
accaaaaaa gatcaaaaa actggcaaa aaacccaaa 
caaagtctc ttccttcga gtcattctg tgcttttct 
gcaatcgac aatgggaaa aaaatcctc aatgtggaa 
aaagtctcg caggattcg atcaaatgc atccacggt 
ctgcgtttc ttctccatc gcttggatc atcatggtc 
cacacttac ctcgaagtt ttctcaatt ggtgacaat 
aaaaatttg aggattttg actgagcgc aacttcgtg 
tatcaaacc gtctctaat gcgactttt tccgtggac 
accttcttc ttcatcagc ggcttgctg gtcacaatc 
acttacttc cgaaccgcg gccaaaaaa ccggccaag 
gaagagaac acgtgcaga tcagcccag accaatttt 
ttcaaattc gtgatgcta gtcatttac cgattcttc 
cgcctcaca cctgcttat ctcttcgtg ttgggtgtg 
aatgaagtg attttgcgc tacttgcac agttattcg 
gtcttttca ccggcgatt attgaccac atcagttgt 
agcaacttc tggtggagg aatgctctc tatattaac 
aatttttac ccccaaacg gagttttgt atgctttgg 
tcgtggtat atcgccaat gatacgcag ttttatctc 
gtggctagt gtgcttctc ctgattgca gtgcgaggt 
gagaaacac ttgaagttc gccggtgcc gcaattggc 
gtcttcatg gtgtcctcc tggatcatg actttcatc 
atagcgatg aaatacgag tacgttgta agagtggaa 
gaaccattt gcgctcttt gaccaactt tacgacaag 
ccctggatg cggataggc ccgtatttc gtcggaatg 
atctccggg tacttcctc ttcagggtg aaatgccaa 
gtcaagctt ccactagtt gtggtagta ctgggatgg 
gtactatcc ctggcttgt ttatccagt ttagtctac 
ggtgttggt aaactaggc ctggtagta ccaacatca 
gcgttttac gcttctttg gggcacaca gcgtggggc 
ttgtccctg gcctggatc acactggcg tgctgctcg 
ggctacggg ggcccgatc agtgctctc ttaaaattc 
aaattcttt ctacctctc agtagatta acttattgc 
gcatatttg atacatcct gtatttatg tgtttgagc 
agcttcgtc ttagatgga ccaattcat ctcaacaac 
agtcttgtc atggtaata tattgtggc aacattgtg 
gcctcattc gtgtcagca ttcgccatt tcgctcgct 
ttcgaagca ccgatcgta aatttgctc aaaattatt 
ttttaagtg ttttagacg taaagttgt atattttgt 
aaaaagcgt attacaaat aaaatagtt aaggaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY