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Gene information
General information
TC011151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3841177..3854058
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011151
    iBeetle screen iB_06855

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: larvae die after hatching
    larval musculature random rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: probably dying
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    prothorax cuticle potentially posteriordecreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax anterior ventralbent - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax partially anteriorconstricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen ventral potentiallynot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011151 RA
      MAQTPLVLL LLLCVSSAL TEPTRYNSS DLSYLSNGI 
      QFPVPASEL HNPAVEEIF QDVSEVRWL AELFDHLKW 
      AGKLKVLNE TRCQTDLEL YVEELLNGT SWAAKMYDS 
      SGRYSGQFF FGNDYWLGS KTLCEELTN PETNPEIPP 
      FPVTFLMAK VRLNINRNL TPVTRQLNV GECLPSSCT 
      PADVRLLLA QDRKAGSTL NVVGIRPVP GDYRLLEDI 
      KFQIVGGVT LVVSLLIFV ASLVEIIAN KKQKQKVDA 
      ELENNNNVD DAKSTKKDQ KTGKKPKQS LFLRVILCF 
      SAIDNGKKI LNVEKVSQD SIKCIHGLR FFSIAWIIM 
      VHTYLEVFS IGDNKNLRI LTERNFVYQ TVSNATFSV 
      DTFFFISGL LVTITYFRT AAKKPAKEE NTCRSAQTN 
      FFKFVMLVI YRFFRLTPA YLFVLGVNE VILRYLHSY 
      SVFSPAIID HISCSNFWW RNALYINNF YPQTEFCML 
      WSWYIANDT QFYLVASVL LLIAVRGEK HLKFAGAAI 
      GVFMVSSWI MTFIIAMKY EYVVRVEEP FALFDQLYD 
      KPWMRIGPY FVGMISGYF LFRVKCQVK LPLVVVVLG 
      WVLSLACLS SLVYGVGKL GLVVPTSAF YASLGHTAW 
      GLSLAWITL ACCSGYGGP ISALLKFKF FLPLSRLTY 
      CAYLIHPVF MCLSSFVLD GPIHLNNSL VMVIYCGNI 
      VASFVSAFA ISLAFEAPI VNLLKIIF
    CDS sequence
    • >TC011151 RA
      atggcccaa acgcccctc gtgctgctg ctgttgttg 
      tgtgtgagc agtgcccta accgaaccg acaagatac 
      aattcgtcc gatctttcc tacctatca aacggtatt 
      cagttcccg gttccggcc tcagagttg cataatcca 
      gctgtagag gaaattttt caggacgtg tcggaagtc 
      cggtggttg gccgaactg ttcgaccac ctaaaatgg 
      gccggaaag ctcaaagtg ctgaacgag acgaggtgt 
      cagacagac ctggagctg tatgtcgaa gagttgctg 
      aatgggact tcctgggcg gccaaaatg tacgactca 
      tctggtcgc tactcgggc cagttcttc ttcggtaac 
      gactactgg ctgggttcg aaaaccctc tgcgaagag 
      ctaaccaac cctgagacc aacccggag atccctccc 
      tttcctgtc accttcctg atggccaaa gttcggctc 
      aacatcaac cgaaatcta acaccagtg accagacag 
      ttgaatgtc ggcgaatgt ctgccatct tcttgcact 
      cctgcagac gtacgattg ctgctggcg caggatcgc 
      aaagctggg tcaacactc aacgtggtg ggaattcgg 
      ccggttccg ggggattac aggcttctg gaggatatc 
      aaatttcaa attgtggga ggtgtcaca ctggtcgtc 
      agcctcctc attttcgtc gcatctttg gtcgaaatc 
      atcgcaaac aagaagcag aaacagaaa gtggacgcc 
      gaacttgaa aacaacaac aatgtggac gatgccaaa 
      agcaccaaa aaagatcaa aaaactggc aaaaaaccc 
      aaacaaagt ctcttcctt cgagtcatt ctgtgcttt 
      tctgcaatc gacaatggg aaaaaaatc ctcaatgtg 
      gaaaaagtc tcgcaggat tcgatcaaa tgcatccac 
      ggtctgcgt ttcttctcc atcgcttgg atcatcatg 
      gtccacact tacctcgaa gttttctca attggtgac 
      aataaaaat ttgaggatt ttgactgag cgcaacttc 
      gtgtatcaa accgtctct aatgcgact ttttccgtg 
      gacaccttc ttcttcatc agcggcttg ctggtcaca 
      atcacttac ttccgaacc gcggccaaa aaaccggcc 
      aaggaagag aacacgtgc agatcagcc cagaccaat 
      tttttcaaa ttcgtgatg ctagtcatt taccgattc 
      ttccgcctc acacctgct tatctcttc gtgttgggt 
      gtgaatgaa gtgattttg cgctacttg cacagttat 
      tcggtcttt tcaccggcg attattgac cacatcagt 
      tgtagcaac ttctggtgg aggaatgct ctctatatt 
      aacaatttt tacccccaa acggagttt tgtatgctt 
      tggtcgtgg tatatcgcc aatgatacg cagttttat 
      ctcgtggct agtgtgctt ctcctgatt gcagtgcga 
      ggtgagaaa cacttgaag ttcgccggt gccgcaatt 
      ggcgtcttc atggtgtcc tcctggatc atgactttc 
      atcatagcg atgaaatac gagtacgtt gtaagagtg 
      gaagaacca tttgcgctc tttgaccaa ctttacgac 
      aagccctgg atgcggata ggcccgtat ttcgtcgga 
      atgatctcc gggtacttc ctcttcagg gtgaaatgc 
      caagtcaag cttccacta gttgtggta gtactggga 
      tgggtacta tccctggct tgtttatcc agtttagtc 
      tacggtgtt ggtaaacta ggcctggta gtaccaaca 
      tcagcgttt tacgcttct ttggggcac acagcgtgg 
      ggcttgtcc ctggcctgg atcacactg gcgtgctgc 
      tcgggctac gggggcccg atcagtgct ctcttaaaa 
      ttcaaattc tttctacct ctcagtaga ttaacttat 
      tgcgcatat ttgatacat cctgtattt atgtgtttg 
      agcagcttc gtcttagat ggaccaatt catctcaac 
      aacagtctt gtcatggta atatattgt ggcaacatt 
      gtggcctca ttcgtgtca gcattcgcc atttcgctc 
      gctttcgaa gcaccgatc gtaaatttg ctcaaaatt 
      attttttaa
    mRNA sequence
    • >TC011151 RA
      aatttcaaa atcgcctca gtcctaagc cgaatcgaa 
      agtcgcgtc agtgtgcac ccagcagtg ataacgtcc 
      acacggtcc aaaatgccc acataaccc tctcgcgaa 
      ccagactgt gctctagta ccgtgtgtc ccaaaaatg 
      gcccaaacg cccctcgtg ctgctgctg ttgttgtgt 
      gtgagcagt gccctaacc gaaccgaca agatacaat 
      tcgtccgat ctttcctac ctatcaaac ggtattcag 
      ttcccggtt ccggcctca gagttgcat aatccagct 
      gtagaggaa atttttcag gacgtgtcg gaagtccgg 
      tggttggcc gaactgttc gaccaccta aaatgggcc 
      ggaaagctc aaagtgctg aacgagacg aggtgtcag 
      acagacctg gagctgtat gtcgaagag ttgctgaat 
      gggacttcc tgggcggcc aaaatgtac gactcatct 
      ggtcgctac tcgggccag ttcttcttc ggtaacgac 
      tactggctg ggttcgaaa accctctgc gaagagcta 
      accaaccct gagaccaac ccggagatc cctcccttt 
      cctgtcacc ttcctgatg gccaaagtt cggctcaac 
      atcaaccga aatctaaca ccagtgacc agacagttg 
      aatgtcggc gaatgtctg ccatcttct tgcactcct 
      gcagacgta cgattgctg ctggcgcag gatcgcaaa 
      gctgggtca acactcaac gtggtggga attcggccg 
      gttccgggg gattacagg cttctggag gatatcaaa 
      tttcaaatt gtgggaggt gtcacactg gtcgtcagc 
      ctcctcatt ttcgtcgca tctttggtc gaaatcatc 
      gcaaacaag aagcagaaa cagaaagtg gacgccgaa 
      cttgaaaac aacaacaat gtggacgat gccaaaagc 
      accaaaaaa gatcaaaaa actggcaaa aaacccaaa 
      caaagtctc ttccttcga gtcattctg tgcttttct 
      gcaatcgac aatgggaaa aaaatcctc aatgtggaa 
      aaagtctcg caggattcg atcaaatgc atccacggt 
      ctgcgtttc ttctccatc gcttggatc atcatggtc 
      cacacttac ctcgaagtt ttctcaatt ggtgacaat 
      aaaaatttg aggattttg actgagcgc aacttcgtg 
      tatcaaacc gtctctaat gcgactttt tccgtggac 
      accttcttc ttcatcagc ggcttgctg gtcacaatc 
      acttacttc cgaaccgcg gccaaaaaa ccggccaag 
      gaagagaac acgtgcaga tcagcccag accaatttt 
      ttcaaattc gtgatgcta gtcatttac cgattcttc 
      cgcctcaca cctgcttat ctcttcgtg ttgggtgtg 
      aatgaagtg attttgcgc tacttgcac agttattcg 
      gtcttttca ccggcgatt attgaccac atcagttgt 
      agcaacttc tggtggagg aatgctctc tatattaac 
      aatttttac ccccaaacg gagttttgt atgctttgg 
      tcgtggtat atcgccaat gatacgcag ttttatctc 
      gtggctagt gtgcttctc ctgattgca gtgcgaggt 
      gagaaacac ttgaagttc gccggtgcc gcaattggc 
      gtcttcatg gtgtcctcc tggatcatg actttcatc 
      atagcgatg aaatacgag tacgttgta agagtggaa 
      gaaccattt gcgctcttt gaccaactt tacgacaag 
      ccctggatg cggataggc ccgtatttc gtcggaatg 
      atctccggg tacttcctc ttcagggtg aaatgccaa 
      gtcaagctt ccactagtt gtggtagta ctgggatgg 
      gtactatcc ctggcttgt ttatccagt ttagtctac 
      ggtgttggt aaactaggc ctggtagta ccaacatca 
      gcgttttac gcttctttg gggcacaca gcgtggggc 
      ttgtccctg gcctggatc acactggcg tgctgctcg 
      ggctacggg ggcccgatc agtgctctc ttaaaattc 
      aaattcttt ctacctctc agtagatta acttattgc 
      gcatatttg atacatcct gtatttatg tgtttgagc 
      agcttcgtc ttagatgga ccaattcat ctcaacaac 
      agtcttgtc atggtaata tattgtggc aacattgtg 
      gcctcattc gtgtcagca ttcgccatt tcgctcgct 
      ttcgaagca ccgatcgta aatttgctc aaaattatt 
      ttttaagtg ttttagacg taaagttgt atattttgt 
      aaaaagcgt attacaaat aaaatagtt aaggaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY