General information | |||
TC011151 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 3841177..3854058 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011151 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06855 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)Comment: larvae die after hatching |
Comment: probably dying |
prothorax cuticle potentially posteriordecreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax anterior ventralbent - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax partially anteriorconstricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen ventral potentiallynot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC011151 RA
MAQTPLVLL LLLCVSSAL TEPTRYNSS DLSYLSNGI QFPVPASEL HNPAVEEIF QDVSEVRWL AELFDHLKW AGKLKVLNE TRCQTDLEL YVEELLNGT SWAAKMYDS SGRYSGQFF FGNDYWLGS KTLCEELTN PETNPEIPP FPVTFLMAK VRLNINRNL TPVTRQLNV GECLPSSCT PADVRLLLA QDRKAGSTL NVVGIRPVP GDYRLLEDI KFQIVGGVT LVVSLLIFV ASLVEIIAN KKQKQKVDA ELENNNNVD DAKSTKKDQ KTGKKPKQS LFLRVILCF SAIDNGKKI LNVEKVSQD SIKCIHGLR FFSIAWIIM VHTYLEVFS IGDNKNLRI LTERNFVYQ TVSNATFSV DTFFFISGL LVTITYFRT AAKKPAKEE NTCRSAQTN FFKFVMLVI YRFFRLTPA YLFVLGVNE VILRYLHSY SVFSPAIID HISCSNFWW RNALYINNF YPQTEFCML WSWYIANDT QFYLVASVL LLIAVRGEK HLKFAGAAI GVFMVSSWI MTFIIAMKY EYVVRVEEP FALFDQLYD KPWMRIGPY FVGMISGYF LFRVKCQVK LPLVVVVLG WVLSLACLS SLVYGVGKL GLVVPTSAF YASLGHTAW GLSLAWITL ACCSGYGGP ISALLKFKF FLPLSRLTY CAYLIHPVF MCLSSFVLD GPIHLNNSL VMVIYCGNI VASFVSAFA ISLAFEAPI VNLLKIIF
- >TC011151 RA
atggcccaa acgcccctc gtgctgctg ctgttgttg tgtgtgagc agtgcccta accgaaccg acaagatac aattcgtcc gatctttcc tacctatca aacggtatt cagttcccg gttccggcc tcagagttg cataatcca gctgtagag gaaattttt caggacgtg tcggaagtc cggtggttg gccgaactg ttcgaccac ctaaaatgg gccggaaag ctcaaagtg ctgaacgag acgaggtgt cagacagac ctggagctg tatgtcgaa gagttgctg aatgggact tcctgggcg gccaaaatg tacgactca tctggtcgc tactcgggc cagttcttc ttcggtaac gactactgg ctgggttcg aaaaccctc tgcgaagag ctaaccaac cctgagacc aacccggag atccctccc tttcctgtc accttcctg atggccaaa gttcggctc aacatcaac cgaaatcta acaccagtg accagacag ttgaatgtc ggcgaatgt ctgccatct tcttgcact cctgcagac gtacgattg ctgctggcg caggatcgc aaagctggg tcaacactc aacgtggtg ggaattcgg ccggttccg ggggattac aggcttctg gaggatatc aaatttcaa attgtggga ggtgtcaca ctggtcgtc agcctcctc attttcgtc gcatctttg gtcgaaatc atcgcaaac aagaagcag aaacagaaa gtggacgcc gaacttgaa aacaacaac aatgtggac gatgccaaa agcaccaaa aaagatcaa aaaactggc aaaaaaccc aaacaaagt ctcttcctt cgagtcatt ctgtgcttt tctgcaatc gacaatggg aaaaaaatc ctcaatgtg gaaaaagtc tcgcaggat tcgatcaaa tgcatccac ggtctgcgt ttcttctcc atcgcttgg atcatcatg gtccacact tacctcgaa gttttctca attggtgac aataaaaat ttgaggatt ttgactgag cgcaacttc gtgtatcaa accgtctct aatgcgact ttttccgtg gacaccttc ttcttcatc agcggcttg ctggtcaca atcacttac ttccgaacc gcggccaaa aaaccggcc aaggaagag aacacgtgc agatcagcc cagaccaat tttttcaaa ttcgtgatg ctagtcatt taccgattc ttccgcctc acacctgct tatctcttc gtgttgggt gtgaatgaa gtgattttg cgctacttg cacagttat tcggtcttt tcaccggcg attattgac cacatcagt tgtagcaac ttctggtgg aggaatgct ctctatatt aacaatttt tacccccaa acggagttt tgtatgctt tggtcgtgg tatatcgcc aatgatacg cagttttat ctcgtggct agtgtgctt ctcctgatt gcagtgcga ggtgagaaa cacttgaag ttcgccggt gccgcaatt ggcgtcttc atggtgtcc tcctggatc atgactttc atcatagcg atgaaatac gagtacgtt gtaagagtg gaagaacca tttgcgctc tttgaccaa ctttacgac aagccctgg atgcggata ggcccgtat ttcgtcgga atgatctcc gggtacttc ctcttcagg gtgaaatgc caagtcaag cttccacta gttgtggta gtactggga tgggtacta tccctggct tgtttatcc agtttagtc tacggtgtt ggtaaacta ggcctggta gtaccaaca tcagcgttt tacgcttct ttggggcac acagcgtgg ggcttgtcc ctggcctgg atcacactg gcgtgctgc tcgggctac gggggcccg atcagtgct ctcttaaaa ttcaaattc tttctacct ctcagtaga ttaacttat tgcgcatat ttgatacat cctgtattt atgtgtttg agcagcttc gtcttagat ggaccaatt catctcaac aacagtctt gtcatggta atatattgt ggcaacatt gtggcctca ttcgtgtca gcattcgcc atttcgctc gctttcgaa gcaccgatc gtaaatttg ctcaaaatt attttttaa
- >TC011151 RA
aatttcaaa atcgcctca gtcctaagc cgaatcgaa agtcgcgtc agtgtgcac ccagcagtg ataacgtcc acacggtcc aaaatgccc acataaccc tctcgcgaa ccagactgt gctctagta ccgtgtgtc ccaaaaatg gcccaaacg cccctcgtg ctgctgctg ttgttgtgt gtgagcagt gccctaacc gaaccgaca agatacaat tcgtccgat ctttcctac ctatcaaac ggtattcag ttcccggtt ccggcctca gagttgcat aatccagct gtagaggaa atttttcag gacgtgtcg gaagtccgg tggttggcc gaactgttc gaccaccta aaatgggcc ggaaagctc aaagtgctg aacgagacg aggtgtcag acagacctg gagctgtat gtcgaagag ttgctgaat gggacttcc tgggcggcc aaaatgtac gactcatct ggtcgctac tcgggccag ttcttcttc ggtaacgac tactggctg ggttcgaaa accctctgc gaagagcta accaaccct gagaccaac ccggagatc cctcccttt cctgtcacc ttcctgatg gccaaagtt cggctcaac atcaaccga aatctaaca ccagtgacc agacagttg aatgtcggc gaatgtctg ccatcttct tgcactcct gcagacgta cgattgctg ctggcgcag gatcgcaaa gctgggtca acactcaac gtggtggga attcggccg gttccgggg gattacagg cttctggag gatatcaaa tttcaaatt gtgggaggt gtcacactg gtcgtcagc ctcctcatt ttcgtcgca tctttggtc gaaatcatc gcaaacaag aagcagaaa cagaaagtg gacgccgaa cttgaaaac aacaacaat gtggacgat gccaaaagc accaaaaaa gatcaaaaa actggcaaa aaacccaaa caaagtctc ttccttcga gtcattctg tgcttttct gcaatcgac aatgggaaa aaaatcctc aatgtggaa aaagtctcg caggattcg atcaaatgc atccacggt ctgcgtttc ttctccatc gcttggatc atcatggtc cacacttac ctcgaagtt ttctcaatt ggtgacaat aaaaatttg aggattttg actgagcgc aacttcgtg tatcaaacc gtctctaat gcgactttt tccgtggac accttcttc ttcatcagc ggcttgctg gtcacaatc acttacttc cgaaccgcg gccaaaaaa ccggccaag gaagagaac acgtgcaga tcagcccag accaatttt ttcaaattc gtgatgcta gtcatttac cgattcttc cgcctcaca cctgcttat ctcttcgtg ttgggtgtg aatgaagtg attttgcgc tacttgcac agttattcg gtcttttca ccggcgatt attgaccac atcagttgt agcaacttc tggtggagg aatgctctc tatattaac aatttttac ccccaaacg gagttttgt atgctttgg tcgtggtat atcgccaat gatacgcag ttttatctc gtggctagt gtgcttctc ctgattgca gtgcgaggt gagaaacac ttgaagttc gccggtgcc gcaattggc gtcttcatg gtgtcctcc tggatcatg actttcatc atagcgatg aaatacgag tacgttgta agagtggaa gaaccattt gcgctcttt gaccaactt tacgacaag ccctggatg cggataggc ccgtatttc gtcggaatg atctccggg tacttcctc ttcagggtg aaatgccaa gtcaagctt ccactagtt gtggtagta ctgggatgg gtactatcc ctggcttgt ttatccagt ttagtctac ggtgttggt aaactaggc ctggtagta ccaacatca gcgttttac gcttctttg gggcacaca gcgtggggc ttgtccctg gcctggatc acactggcg tgctgctcg ggctacggg ggcccgatc agtgctctc ttaaaattc aaattcttt ctacctctc agtagatta acttattgc gcatatttg atacatcct gtatttatg tgtttgagc agcttcgtc ttagatgga ccaattcat ctcaacaac agtcttgtc atggtaata tattgtggc aacattgtg gcctcattc gtgtcagca ttcgccatt tcgctcgct ttcgaagca ccgatcgta aatttgctc aaaattatt ttttaagtg ttttagacg taaagttgt atattttgt aaaaagcgt attacaaat aaaatagtt aaggaaag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
