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Gene information
General information
TC001316 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14435803..14437822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001316
    iBeetle screen iB_06844

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001316 RA
      MNVFFSHVA KRGFSTSNF NNAKHSCKV LVVGGGTGG 
      CATAAKFSK ILKKNELII LEPSENHYY QPLFTLIGG 
      GISSLSDAR KNTKEVLPK NAVWLKDSA AEFNPQSNS 
      VTTQNGDTI EYEFLLVAT GLQTDYDKI PGLVAGLNS 
      DTGVCSNYS AKYVEGTYR ALQKFENGN AIFTFPNSP 
      VKCPGAPQK ICYITDHFL RKVGKRDKA NVIYNTSLP 
      VIFGVKKYA DALWKVCEE RNINVNLRT NLIEVRPDK 
      REAVFQNLD KPEQVTTFE YSMLHVTPP MSTPPALRT 
      CRSLTNESG FVDVDKSTL QSTKFSNVF AIGDCSSSP 
      NSKTAAAAA AQCQVVFKN MKSVMDGKT PVSAYDGYA 
      SCPLVTGYS TCILAEFDY NLAPLETFP FNQGRELWS 
      MYLMKKMLM PPLYWNLML NGWWNGPAL VRNLLHLRF 
      PQN
    CDS sequence
    • >TC001316 RA
      atgaacgtg tttttttcg catgtcgct aagcggggc 
      ttttctacc tcgaacttt aacaatgct aaacacagt 
      tgcaaggtc ctggtcgtg ggtgggggc accggtggc 
      tgtgccacg gccgcaaaa ttcagtaaa atcctcaaa 
      aagaacgag ctcataatc ctagaaccc agcgaaaat 
      cactactat cagcctctg tttacttta attgggggc 
      ggcatttct agtctctct gatgcaagg aaaaataca 
      aaagaggtt ctcccaaaa aacgcagtt tggctgaaa 
      gactcagct gccgaattc aacccccag tcaaattcc 
      gtcacgacc cagaatggc gatacaatc gaatacgag 
      tttctttta gtcgccact ggtcttcaa accgattat 
      gataaaatt ccaggtcta gtcgctggt ttaaacagt 
      gacacaggg gtgtgctct aattactcg gcaaagtac 
      gtcgaaggg acgtatagg gcgttacaa aagtttgaa 
      aacgggaat gctattttc acgtttcca aacagcccg 
      gttaaatgt ccgggggcg cctcaaaaa atttgttac 
      atcactgat cattttttg agaaaggtg ggcaagcga 
      gataaggcg aacgtaatt tacaacacg tcacttcct 
      gtaattttc ggcgtaaaa aaatacgct gatgcactt 
      tggaaagtg tgcgaggag cgcaacatc aatgttaat 
      ttacgaacg aatttgatt gaagtcagg ccggacaaa 
      agagaggcg gttttccaa aatttggac aagccggag 
      caagtcaca acttttgag tattccatg ttgcatgtt 
      acgccccct atgagcaca cccccagcc ttaagaacc 
      tgtcgcagt ttaacaaac gagtccggg tttgttgat 
      gtggacaaa tccactcta cagagcaca aaattttct 
      aatgttttt gcaattggt gattgtagt tcaagcccc 
      aattctaaa acggcagcg gctgcagct gctcaatgc 
      caggttgtt tttaaaaat atgaaatcg gttatggat 
      gggaaaacg ccagtttcg gcatacgat ggttatgcc 
      tcttgtcct ctggtgacg gggtacagc acgtgcatt 
      ttggccgaa ttcgattat aatttagct ccgttggaa 
      acgttccct tttaatcaa ggacgggag ttgtggagc 
      atgtattta atgaagaaa atgctaatg cctccgtta 
      tactggaac ttgatgctg aatggctgg tggaatgga 
      ccggccctg gttaggaac ctactccac ttgaggttt 
      ccacaaaat taa
    mRNA sequence
    • >TC001316 RA
      ctacttgtt atttaaagt caagttctt tatctcatc 
      aaacgtttc attattcac gataagaaa atcattgca 
      aaaagtttt ttatagttt attgatttc cattcttat 
      ttctttttg gaacaaata ccgagaaaa tgaacgtgt 
      ttttttcgc atgtcgcta agcggggct tttctacct 
      cgaacttta acaatgcta aacacagtt gcaaggtcc 
      tggtcgtgg gtgggggca ccggtggct gtgccacgg 
      ccgcaaaat tcagtaaaa tcctcaaaa agaacgagc 
      tcataatcc tagaaccca gcgaaaatc actactatc 
      agcctctgt ttactttaa ttgggggcg gcatttcta 
      gtctctctg atgcaagga aaaatacaa aagaggttc 
      tcccaaaaa acgcagttt ggctgaaag actcagctg 
      ccgaattca acccccagt caaattccg tcacgaccc 
      agaatggcg atacaatcg aatacgagt ttcttttag 
      tcgccactg gtcttcaaa ccgattatg ataaaattc 
      caggtctag tcgctggtt taaacagtg acacagggg 
      tgtgctcta attactcgg caaagtacg tcgaaggga 
      cgtataggg cgttacaaa agtttgaaa acgggaatg 
      ctattttca cgtttccaa acagcccgg ttaaatgtc 
      cgggggcgc ctcaaaaaa tttgttaca tcactgatc 
      attttttga gaaaggtgg gcaagcgag ataaggcga 
      acgtaattt acaacacgt cacttcctg taattttcg 
      gcgtaaaaa aatacgctg atgcacttt ggaaagtgt 
      gcgaggagc gcaacatca atgttaatt tacgaacga 
      atttgattg aagtcaggc cggacaaaa gagaggcgg 
      ttttccaaa atttggaca agccggagc aagtcacaa 
      cttttgagt attccatgt tgcatgtta cgcccccta 
      tgagcacac ccccagcct taagaacct gtcgcagtt 
      taacaaacg agtccgggt ttgttgatg tggacaaat 
      ccactctac agagcacaa aattttcta atgtttttg 
      caattggtg attgtagtt caagcccca attctaaaa 
      cggcagcgg ctgcagctg ctcaatgcc aggttgttt 
      ttaaaaata tgaaatcgg ttatggatg ggaaaacgc 
      cagtttcgg catacgatg gttatgcct cttgtcctc 
      tggtgacgg ggtacagca cgtgcattt tggccgaat 
      tcgattata atttagctc cgttggaaa cgttccctt 
      ttaatcaag gacgggagt tgtggagca tgtatttaa 
      tgaagaaaa tgctaatgc ctccgttat actggaact 
      tgatgctga atggctggt ggaatggac cggccctgg 
      ttaggaacc tactccact tgaggtttc cacaaaatt 
      aaggaataa aacgatgca ataaatgtt gtcctttgt 
      tgtgaattt tattattta taatattgt gtacacgta 
      tggaaataa agctatttt atcaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY