| General information | |||
| TC031006
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 13713491..13719055 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031006 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06833 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031006 RA
MHFLKKYPK IVKFTCLAT VSFGAGWLY YQVKTTKKI LSNALDTSI LSIIEPTAV EKSKDDDFF VIDITGDPN RQETYLQKW TALKFSFAK RLLVLASSE NRFLRKRAV RQLARIKKL ESWQYSQLA DMIDARTAV GLARTPDVE PRLFNNPPL RFLGHTHDM IVDVMKDFL VNLHAMSQH PCMGYFISK AFLDLHEVE KFGDDITPY ELKNFIQTS EELLPTCLE SLLHHASLG KYAKDIAEL NGLPLLMEI HNRFKDNLS VTATICRII SYLSMHRNL LDEMYKSGW VGLLAQWVK HDDVRISIP ASKALANLD CDEDPVYLQ RLYPLHPST RIVQTPKVD VVFIHGLLG GVFFTWRQR QRNQDPLGF LGKKGAPES LTQNNSTCI NKQCTEKRN KIQRISDPQ MREFLEEFE THTAYGCLC NDYDFVWED IPVNTNETA TGPYCCSGF KYVCMEKSE DECYTNCWP RDWLAQDCD RLRIIGVNY ETNLSLWTP ICPVEKVKT LEERSDELI EQLERVEVG KRPIVWVTH SMGGLMVKC LLNKASENG NKRIRDLYT NTRGIIFYS TPHLGSSVA SFSQASALV IWPSIEVQE LQKHSPQLV QIHQKFLRL IKNHPMKIV TFVETKPTV VSAMKFKFL VVEPDSGNP GYGEYYEIP LDHLGICKP ASRHSFLYQ KVLHMITSI LEESGPDIS AFSDDNFDT KAWINDVLK NAETQDKKE NYTMSLVMK LQLCVQQVN SALEDTSQQ ILMSLPKII RDTKNLNQE AAILKEKMA AVRSEIVKI EQDTGKSIN TIEKLDTIK NELNMAKQG LHESDNWTI LVNDLEEVF DSKNIENIS AKIIGMQRS LQLLVNVSD YEDRKLQLE GLKNRLEAI ASPAIVQAF TSSNTEQSS MFVRIFKSM GRLPQLLKY YHKCQKDVL LKKWRNQLE IEQDESVIQ WIHNFYGIM LSNWHTQQK WFNQVFTNQ NSCESFVEI YTDVLTSLD PTLNECIDA ALKQVEDKL NFLFEIKQI TQQFADNLT DVINQSPGG KLAHDKCLL LLQAVYNHL IPYTSKYAA YEQAHLMKK LSAINCMKE ELSDTIQAL GLSIPQIID IARDAKKRC QKITENCGY CGLLIALRA FLLSYADQY RVALRQIDR NKRQEEDWG TFQLCLSLL QNTGEVLVN LQQLEKDLT GTILETNQI KAQFEYKFL LLSASDRKE YESLVRCVT EGTQLSLLD HVNNEFSKL CSDIHHTTY LVIFAPISL HLDVVQVPK TWAQFANSM HNDLPDYSF TPQEYITQI GQYLMTLPQ HLEPFLFRD NPSLSCALK AVDQEYSTA GETEGALAH VFLTFIARR TCQGFCERI LSICELSQA ASRQLAHDI GYLENVLQD LGISIS
- >TC031006 RA
atgcatttt ttaaaaaag tatccaaaa attgttaaa tttacatgt ttggctaca gtttcattc ggcgcagga tggttatat tatcaggta aaaacgacc aaaaaaatc ctcagcaat gccttagac acatcaatt ttatcaata atcgagcca acagccgtg gaaaaatcc aaagatgat gactttttc gtcattgat ataactggg gacccgaac cggcaagaa acctattta caaaaatgg accgctttg aagttttca ttcgctaaa agattgctt gttcttgca agtagtgaa aatcggttt cttcggaaa agggctgtg agacagttg gctaggatt aaaaaattg gaaagctgg caatactcg caactcgct gatatgatt gatgccagg actgcggta gggctggct aggacaccc gatgttgag cctagatta ttcaacaac ccgccctta agatttctc ggtcatacg cacgacatg attgtcgat gtgatgaaa gatttttta gtcaattta cacgcgatg tctcaacac ccttgtatg ggttatttc atctctaag gcatttctc gacttgcat gaagttgag aaattcggt gatgacata actccctac gagttgaaa aacttcatt caaaccagt gaagaactc ctaccgacg tgtctagag tcactttta caccacgcc agtttaggg aaatacgcc aaagatatt gccgaactc aacggtctt ccactttta atggaaatt cacaaccgg ttcaaagac aacctttct gtcaccgca actatttgt cggattatt tcgtatctt tcaatgcac agaaatctc cttgacgaa atgtataaa tcgggttgg gtcgggctt ttggcccag tgggtcaaa cacgacgac gtccgaata tcaatccct gcatctaaa gcacttgca aatttagac tgtgatgaa gaccctgtt tacctacaa agactctac cctttgcat ccttcaacc cgtattgta caaaccccg aaagttgac gtcgttttt atccatggt ttactgggg ggtgtattt tttacgtgg aggcagaga caacgcaac caggatccg ttggggttc cttggtaag aagggcgcc ccagaaagt ctgacacag aacaactca acttgtatc aataaacaa tgcaccgaa aagagaaac aaaatccaa agaataagc gatcctcaa atgagggaa tttctggaa gagtttgaa acgcacaca gcttatggt tgcctttgc aacgattat gactttgtt tgggaggac ataccggtc aatacaaat gagactgcc acagggccg tactgttgt tctggcttt aaatacgtc tgtatggaa aaaagtgag gatgaatgt tacaccaat tgctggcct agagactgg ttagcgcaa gattgtgat cgtttgaga atcattggg gtaaattat gagacaaat ctatcgttg tggacaccc atatgtcca gtagagaaa gtcaagact ttggaagag cgaagcgac gagttgatt gaacagttg gagagggtt gaagttggg aaaagaccc atcgtgtgg gtgacacac tcaatgggt ggtttaatg gtcaagtgt ctcctaaat aaagcttcc gaaaatggc aataaacga atcagagat ttgtacaca aacactcga gggataatt ttctatagc acgcctcat ttaggatca agtgttgct agtttcagc caggcttca gcccttgtg atttggcca agtattgaa gtccaggag ttacaaaaa cattcgcca caattggtg caaatacac caaaaattc ctgagactt atcaaaaat catccaatg aaaattgtg acttttgtg gagaccaaa cctacggta gtttcagca atgaaattt aaattttta gtggttgaa cccgactct ggcaatccg gggtatggg gaatactat gagatacct ttagaccat ttgggaatt tgcaaaccg gcaagcaga cattcgttt ctataccaa aaagtgctt cacatgatc acgtccatt ttggaggaa agtgggcca gatattagc gcgttctct gatgataat ttcgatacc aaagcgtgg ataaacgat gttttaaaa aatgcagag acccaggac aagaaagag aattataca atgtccctc gttatgaag ctccaacta tgcgtccaa caagtgaac agtgccctt gaagacacc agtcaacaa atcctaatg tcactgcca aaaatcatc agagacacc aaaaatctc aaccaggaa gcagcaatt ttaaaggaa aaaatggca gcagttcgt agcgaaatc gtaaaaatt gaacaagac actggaaag tctataaac acaattgag aaactggac actattaag aatgaactg aatatggcc aagcagggg ctgcatgag agtgataat tggactatt ttagtcaac gacttggag gaagtcttt gattccaaa aacattgaa aacatttcc gcaaaaata ataggaatg cagcggtct ttgcaactt ttagtcaat gttagtgat tatgaagat cgtaaatta cagttggaa gggctgaaa aatcggctg gaagcaatc gcaagtcca gccatagtc caggctttc acttcttcc aacacagaa caatcgtca atgtttgtg cgaatattt aaatcaatg ggacgctta ccacaactt ctaaagtac taccacaag tgccagaag gacgtttta ttgaaaaag tggcgcaat caattggaa atcgaacaa gacgagtct gtgatccaa tggatccac aatttttac ggcattatg ctgtccaac tggcacacg caacaaaaa tggttcaac caagtcttc accaaccaa aacagttgc gaatcattc gttgagatt tacaccgac gttctcacg tctttagac ccgactctc aacgaatgc attgacgcc gctttaaaa caagtcgaa gataaatta aactttctc ttcgaaatc aaacaaatc acccaacag ttcgccgac aacttaacg gacgtcatt aaccaatca ccgggcggc aaactagcc catgacaag tgcttgctg ctactacaa gcagtctac aaccactta atcccatac actagcaaa tacgcggct tatgaacaa gcccaccta atgaaaaaa ctctcggcc ataaattgc atgaaagag gagctttct gatacaatc caagccctt ggcttaagc atccctcaa ataatcgac attgcacgg gacgccaaa aaacgctgc cagaaaata accgaaaat tgcggttac tgtggcttg ttaattgca cttcgggcg tttctatta agctatgcg gaccaatat cgcgtcgct ttgcgacaa atcgatcga aataaaaga caagaggag gattggggc acgtttcag ttgtgtcta tcgttactg cagaacacg ggcgaagtc ctggtcaat ttgcaacag ctggagaag gacctgacc gggactata ctcgaaact aatcaaatt aaagcccag tttgagtac aagttttta ctattaagt gcctctgat cgaaaggaa tacgaatct ttggtcagg tgtgtcact gaagggacg caattgtcg ctacttgac cacgttaat aatgagttt agtaaattg tgctcggac atacaccat acgacttat cttgtcatt ttcgcaccg atttcgcta catttggac gtggtacaa gtgccgaaa acgtgggca caattcgca aattcaatg cacaatgac ttgcccgat tatagtttc acgccacag gagtacatc acacagatc ggtcagtac ttgatgacg ttaccgcaa cacctggaa ccattcctc ttccgagac aacccgtca cttagctgc gccctgaag gcggtggat caggagtac agcactgcc ggagagacg gaaggagcc ctagctcat gtcttcctc acgtttatc gccaggagg acgtgtcag ggattttgc gaacgcatt ttgtcaatt tgtgaattg agccaagcg gccagccga caattggcc cacgatatt ggttacttg gaaaatgtc ttgcaggat ttggggatt tctatcagt tag
- >TC031006 RA
cttacaagc tgcaaaacg tcaaatttg acacttctt cgtgacatg tgcgtttga cattcccat gcatttttt aaaaaagta tccaaaaat tgttaaatt tacatgttt ggctacagt ttcattcgg cgcaggatg gttatatta tcaggtaaa aacgaccaa aaaaatcct cagcaatgc cttagacac atcaatttt atcaataat cgagccaac agccgtgga aaaatccaa agatgatga ctttttcgt cattgatat aactgggga cccgaaccg gcaagaaac ctatttaca aaaatggac cgctttgaa gttttcatt cgctaaaag attgcttgt tcttgcaag tagtgaaaa tcggtttct tcggaaaag ggctgtgag acagttggc taggattaa aaaattgga aagctggca atactcgca actcgctga tatgattga tgccaggac tgcggtagg gctggctag gacacccga tgttgagcc tagattatt caacaaccc gcccttaag atttctcgg tcatacgca cgacatgat tgtcgatgt gatgaaaga ttttttagt caatttaca cgcgatgtc tcaacaccc ttgtatggg ttatttcat ctctaaggc atttctcga cttgcatga agttgagaa attcggtga tgacataac tccctacga gttgaaaaa cttcattca aaccagtga agaactcct accgacgtg tctagagtc acttttaca ccacgccag tttagggaa atacgccaa agatattgc cgaactcaa cggtcttcc acttttaat ggaaattca caaccggtt caaagacaa cctttctgt caccgcaac tatttgtcg gattatttc gtatctttc aatgcacag aaatctcct tgacgaaat gtataaatc gggttgggt cgggctttt ggcccagtg ggtcaaaca cgacgacgt ccgaatatc aatccctgc atctaaagc acttgcaaa tttagactg tgatgaaga ccctgttta cctacaaag actctaccc tttgcatcc ttcaacccg tattgtaca aaccccgaa agttgacgt cgtttttat ccatggttt actgggggg tgtattttt tacgtggag gcagagaca acgcaacca ggatccgtt ggggttcct tggtaagaa gggcgcccc agaaagtct gacacagaa caactcaac ttgtatcaa taaacaatg caccgaaaa gagaaacaa aatccaaag aataagcga tcctcaaat gagggaatt tctggaaga gtttgaaac gcacacagc ttatggttg cctttgcaa cgattatga ctttgtttg ggaggacat accggtcaa tacaaatga gactgccac agggccgta ctgttgttc tggctttaa atacgtctg tatggaaaa aagtgagga tgaatgtta caccaattg ctggcctag agactggtt agcgcaaga ttgtgatcg tttgagaat cattggggt aaattatga gacaaatct atcgttgtg gacacccat atgtccagt agagaaagt caagacttt ggaagagcg aagcgacga gttgattga acagttgga gagggttga agttgggaa aagacccat cgtgtgggt gacacactc aatgggtgg tttaatggt caagtgtct cctaaataa agcttccga aaatggcaa taaacgaat cagagattt gtacacaaa cactcgagg gataatttt ctatagcac gcctcattt aggatcaag tgttgctag tttcagcca ggcttcagc ccttgtgat ttggccaag tattgaagt ccaggagtt acaaaaaca ttcgccaca attggtgca aatacacca aaaattcct gagacttat caaaaatca tccaatgaa aattgtgac ttttgtgga gaccaaacc tacggtagt ttcagcaat gaaatttaa atttttagt ggttgaacc cgactctgg caatccggg gtatgggga atactatga gataccttt agaccattt gggaatttg caaaccggc aagcagaca ttcgtttct ataccaaaa agtgcttca catgatcac gtccatttt ggaggaaag tgggccaga tattagcgc gttctctga tgataattt cgataccaa agcgtggat aaacgatgt tttaaaaaa tgcagagac ccaggacaa gaaagagaa ttatacaat gtccctcgt tatgaagct ccaactatg cgtccaaca agtgaacag tgcccttga agacaccag tcaacaaat cctaatgtc actgccaaa aatcatcag agacaccaa aaatctcaa ccaggaagc agcaatttt aaaggaaaa aatggcagc agttcgtag cgaaatcgt aaaaattga acaagacac tggaaagtc tataaacac aattgagaa actggacac tattaagaa tgaactgaa tatggccaa gcaggggct gcatgagag tgataattg gactatttt agtcaacga cttggagga agtctttga ttccaaaaa cattgaaaa catttccgc aaaaataat aggaatgca gcggtcttt gcaactttt agtcaatgt tagtgatta tgaagatcg taaattaca gttggaagg gctgaaaaa tcggctgga agcaatcgc aagtccagc catagtcca ggctttcac ttcttccaa cacagaaca atcgtcaat gtttgtgcg aatatttaa atcaatggg acgcttacc acaacttct aaagtacta ccacaagtg ccagaagga cgttttatt gaaaaagtg gcgcaatca attggaaat cgaacaaga cgagtctgt gatccaatg gatccacaa tttttacgg cattatgct gtccaactg gcacacgca acaaaaatg gttcaacca agtcttcac caaccaaaa cagttgcga atcattcgt tgagattta caccgacgt tctcacgtc tttagaccc gactctcaa cgaatgcat tgacgccgc tttaaaaca agtcgaaga taaattaaa ctttctctt cgaaatcaa acaaatcac ccaacagtt cgccgacaa cttaacgga cgtcattaa ccaatcacc gggcggcaa actagccca tgacaagtg cttgctgct actacaagc agtctacaa ccacttaat cccatacac tagcaaata cgcggctta tgaacaagc ccacctaat gaaaaaact ctcggccat aaattgcat gaaagagga gctttctga tacaatcca agcccttgg cttaagcat ccctcaaat aatcgacat tgcacggga cgccaaaaa acgctgcca gaaaataac cgaaaattg cggttactg tggcttgtt aattgcact tcgggcgtt tctattaag ctatgcgga ccaatatcg cgtcgcttt gcgacaaat cgatcgaaa taaaagaca agaggagga ttggggcac gtttcagtt gtgtctatc gttactgca gaacacggg cgaagtcct ggtcaattt gcaacagct ggagaagga cctgaccgg gactatact cgaaactaa tcaaattaa agcccagtt tgagtacaa gtttttact attaagtgc ctctgatcg aaaggaata cgaatcttt ggtcaggtg tgtcactga agggacgca attgtcgct acttgacca cgttaataa tgagtttag taaattgtg ctcggacat acaccatac gacttatct tgtcatttt cgcaccgat ttcgctaca tttggacgt ggtacaagt gccgaaaac gtgggcaca attcgcaaa ttcaatgca caatgactt gcccgatta tagtttcac gccacagga gtacatcac acagatcgg tcagtactt gatgacgtt accgcaaca cctggaacc attcctctt ccgagacaa cccgtcact tagctgcgc cctgaaggc ggtggatca ggagtacag cactgccgg agagacgga aggagccct agctcatgt cttcctcac gtttatcgc caggaggac gtgtcaggg attttgcga acgcatttt gtcaatttg tgaattgag ccaagcggc cagccgaca attggccca cgatattgg ttacttgga aaatgtctt gcaggattt ggggatttc tatcagtta ggcagtacc ggatgtccg gctaggttt gtagctgca gtccggcaa atgcgaaat ataacatcc a
