Status
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Gene information
General information
TC031006
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030742,TC030721
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13713491..13719055
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031006
    iBeetle screen iB_06833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031006 RA
      MHFLKKYPK IVKFTCLAT VSFGAGWLY YQVKTTKKI 
      LSNALDTSI LSIIEPTAV EKSKDDDFF VIDITGDPN 
      RQETYLQKW TALKFSFAK RLLVLASSE NRFLRKRAV 
      RQLARIKKL ESWQYSQLA DMIDARTAV GLARTPDVE 
      PRLFNNPPL RFLGHTHDM IVDVMKDFL VNLHAMSQH 
      PCMGYFISK AFLDLHEVE KFGDDITPY ELKNFIQTS 
      EELLPTCLE SLLHHASLG KYAKDIAEL NGLPLLMEI 
      HNRFKDNLS VTATICRII SYLSMHRNL LDEMYKSGW 
      VGLLAQWVK HDDVRISIP ASKALANLD CDEDPVYLQ 
      RLYPLHPST RIVQTPKVD VVFIHGLLG GVFFTWRQR 
      QRNQDPLGF LGKKGAPES LTQNNSTCI NKQCTEKRN 
      KIQRISDPQ MREFLEEFE THTAYGCLC NDYDFVWED 
      IPVNTNETA TGPYCCSGF KYVCMEKSE DECYTNCWP 
      RDWLAQDCD RLRIIGVNY ETNLSLWTP ICPVEKVKT 
      LEERSDELI EQLERVEVG KRPIVWVTH SMGGLMVKC 
      LLNKASENG NKRIRDLYT NTRGIIFYS TPHLGSSVA 
      SFSQASALV IWPSIEVQE LQKHSPQLV QIHQKFLRL 
      IKNHPMKIV TFVETKPTV VSAMKFKFL VVEPDSGNP 
      GYGEYYEIP LDHLGICKP ASRHSFLYQ KVLHMITSI 
      LEESGPDIS AFSDDNFDT KAWINDVLK NAETQDKKE 
      NYTMSLVMK LQLCVQQVN SALEDTSQQ ILMSLPKII 
      RDTKNLNQE AAILKEKMA AVRSEIVKI EQDTGKSIN 
      TIEKLDTIK NELNMAKQG LHESDNWTI LVNDLEEVF 
      DSKNIENIS AKIIGMQRS LQLLVNVSD YEDRKLQLE 
      GLKNRLEAI ASPAIVQAF TSSNTEQSS MFVRIFKSM 
      GRLPQLLKY YHKCQKDVL LKKWRNQLE IEQDESVIQ 
      WIHNFYGIM LSNWHTQQK WFNQVFTNQ NSCESFVEI 
      YTDVLTSLD PTLNECIDA ALKQVEDKL NFLFEIKQI 
      TQQFADNLT DVINQSPGG KLAHDKCLL LLQAVYNHL 
      IPYTSKYAA YEQAHLMKK LSAINCMKE ELSDTIQAL 
      GLSIPQIID IARDAKKRC QKITENCGY CGLLIALRA 
      FLLSYADQY RVALRQIDR NKRQEEDWG TFQLCLSLL 
      QNTGEVLVN LQQLEKDLT GTILETNQI KAQFEYKFL 
      LLSASDRKE YESLVRCVT EGTQLSLLD HVNNEFSKL 
      CSDIHHTTY LVIFAPISL HLDVVQVPK TWAQFANSM 
      HNDLPDYSF TPQEYITQI GQYLMTLPQ HLEPFLFRD 
      NPSLSCALK AVDQEYSTA GETEGALAH VFLTFIARR 
      TCQGFCERI LSICELSQA ASRQLAHDI GYLENVLQD 
      LGISIS
    CDS sequence
    • >TC031006 RA
      atgcatttt ttaaaaaag tatccaaaa attgttaaa 
      tttacatgt ttggctaca gtttcattc ggcgcagga 
      tggttatat tatcaggta aaaacgacc aaaaaaatc 
      ctcagcaat gccttagac acatcaatt ttatcaata 
      atcgagcca acagccgtg gaaaaatcc aaagatgat 
      gactttttc gtcattgat ataactggg gacccgaac 
      cggcaagaa acctattta caaaaatgg accgctttg 
      aagttttca ttcgctaaa agattgctt gttcttgca 
      agtagtgaa aatcggttt cttcggaaa agggctgtg 
      agacagttg gctaggatt aaaaaattg gaaagctgg 
      caatactcg caactcgct gatatgatt gatgccagg 
      actgcggta gggctggct aggacaccc gatgttgag 
      cctagatta ttcaacaac ccgccctta agatttctc 
      ggtcatacg cacgacatg attgtcgat gtgatgaaa 
      gatttttta gtcaattta cacgcgatg tctcaacac 
      ccttgtatg ggttatttc atctctaag gcatttctc 
      gacttgcat gaagttgag aaattcggt gatgacata 
      actccctac gagttgaaa aacttcatt caaaccagt 
      gaagaactc ctaccgacg tgtctagag tcactttta 
      caccacgcc agtttaggg aaatacgcc aaagatatt 
      gccgaactc aacggtctt ccactttta atggaaatt 
      cacaaccgg ttcaaagac aacctttct gtcaccgca 
      actatttgt cggattatt tcgtatctt tcaatgcac 
      agaaatctc cttgacgaa atgtataaa tcgggttgg 
      gtcgggctt ttggcccag tgggtcaaa cacgacgac 
      gtccgaata tcaatccct gcatctaaa gcacttgca 
      aatttagac tgtgatgaa gaccctgtt tacctacaa 
      agactctac cctttgcat ccttcaacc cgtattgta 
      caaaccccg aaagttgac gtcgttttt atccatggt 
      ttactgggg ggtgtattt tttacgtgg aggcagaga 
      caacgcaac caggatccg ttggggttc cttggtaag 
      aagggcgcc ccagaaagt ctgacacag aacaactca 
      acttgtatc aataaacaa tgcaccgaa aagagaaac 
      aaaatccaa agaataagc gatcctcaa atgagggaa 
      tttctggaa gagtttgaa acgcacaca gcttatggt 
      tgcctttgc aacgattat gactttgtt tgggaggac 
      ataccggtc aatacaaat gagactgcc acagggccg 
      tactgttgt tctggcttt aaatacgtc tgtatggaa 
      aaaagtgag gatgaatgt tacaccaat tgctggcct 
      agagactgg ttagcgcaa gattgtgat cgtttgaga 
      atcattggg gtaaattat gagacaaat ctatcgttg 
      tggacaccc atatgtcca gtagagaaa gtcaagact 
      ttggaagag cgaagcgac gagttgatt gaacagttg 
      gagagggtt gaagttggg aaaagaccc atcgtgtgg 
      gtgacacac tcaatgggt ggtttaatg gtcaagtgt 
      ctcctaaat aaagcttcc gaaaatggc aataaacga 
      atcagagat ttgtacaca aacactcga gggataatt 
      ttctatagc acgcctcat ttaggatca agtgttgct 
      agtttcagc caggcttca gcccttgtg atttggcca 
      agtattgaa gtccaggag ttacaaaaa cattcgcca 
      caattggtg caaatacac caaaaattc ctgagactt 
      atcaaaaat catccaatg aaaattgtg acttttgtg 
      gagaccaaa cctacggta gtttcagca atgaaattt 
      aaattttta gtggttgaa cccgactct ggcaatccg 
      gggtatggg gaatactat gagatacct ttagaccat 
      ttgggaatt tgcaaaccg gcaagcaga cattcgttt 
      ctataccaa aaagtgctt cacatgatc acgtccatt 
      ttggaggaa agtgggcca gatattagc gcgttctct 
      gatgataat ttcgatacc aaagcgtgg ataaacgat 
      gttttaaaa aatgcagag acccaggac aagaaagag 
      aattataca atgtccctc gttatgaag ctccaacta 
      tgcgtccaa caagtgaac agtgccctt gaagacacc 
      agtcaacaa atcctaatg tcactgcca aaaatcatc 
      agagacacc aaaaatctc aaccaggaa gcagcaatt 
      ttaaaggaa aaaatggca gcagttcgt agcgaaatc 
      gtaaaaatt gaacaagac actggaaag tctataaac 
      acaattgag aaactggac actattaag aatgaactg 
      aatatggcc aagcagggg ctgcatgag agtgataat 
      tggactatt ttagtcaac gacttggag gaagtcttt 
      gattccaaa aacattgaa aacatttcc gcaaaaata 
      ataggaatg cagcggtct ttgcaactt ttagtcaat 
      gttagtgat tatgaagat cgtaaatta cagttggaa 
      gggctgaaa aatcggctg gaagcaatc gcaagtcca 
      gccatagtc caggctttc acttcttcc aacacagaa 
      caatcgtca atgtttgtg cgaatattt aaatcaatg 
      ggacgctta ccacaactt ctaaagtac taccacaag 
      tgccagaag gacgtttta ttgaaaaag tggcgcaat 
      caattggaa atcgaacaa gacgagtct gtgatccaa 
      tggatccac aatttttac ggcattatg ctgtccaac 
      tggcacacg caacaaaaa tggttcaac caagtcttc 
      accaaccaa aacagttgc gaatcattc gttgagatt 
      tacaccgac gttctcacg tctttagac ccgactctc 
      aacgaatgc attgacgcc gctttaaaa caagtcgaa 
      gataaatta aactttctc ttcgaaatc aaacaaatc 
      acccaacag ttcgccgac aacttaacg gacgtcatt 
      aaccaatca ccgggcggc aaactagcc catgacaag 
      tgcttgctg ctactacaa gcagtctac aaccactta 
      atcccatac actagcaaa tacgcggct tatgaacaa 
      gcccaccta atgaaaaaa ctctcggcc ataaattgc 
      atgaaagag gagctttct gatacaatc caagccctt 
      ggcttaagc atccctcaa ataatcgac attgcacgg 
      gacgccaaa aaacgctgc cagaaaata accgaaaat 
      tgcggttac tgtggcttg ttaattgca cttcgggcg 
      tttctatta agctatgcg gaccaatat cgcgtcgct 
      ttgcgacaa atcgatcga aataaaaga caagaggag 
      gattggggc acgtttcag ttgtgtcta tcgttactg 
      cagaacacg ggcgaagtc ctggtcaat ttgcaacag 
      ctggagaag gacctgacc gggactata ctcgaaact 
      aatcaaatt aaagcccag tttgagtac aagttttta 
      ctattaagt gcctctgat cgaaaggaa tacgaatct 
      ttggtcagg tgtgtcact gaagggacg caattgtcg 
      ctacttgac cacgttaat aatgagttt agtaaattg 
      tgctcggac atacaccat acgacttat cttgtcatt 
      ttcgcaccg atttcgcta catttggac gtggtacaa 
      gtgccgaaa acgtgggca caattcgca aattcaatg 
      cacaatgac ttgcccgat tatagtttc acgccacag 
      gagtacatc acacagatc ggtcagtac ttgatgacg 
      ttaccgcaa cacctggaa ccattcctc ttccgagac 
      aacccgtca cttagctgc gccctgaag gcggtggat 
      caggagtac agcactgcc ggagagacg gaaggagcc 
      ctagctcat gtcttcctc acgtttatc gccaggagg 
      acgtgtcag ggattttgc gaacgcatt ttgtcaatt 
      tgtgaattg agccaagcg gccagccga caattggcc 
      cacgatatt ggttacttg gaaaatgtc ttgcaggat 
      ttggggatt tctatcagt tag
    mRNA sequence
    • >TC031006 RA
      cttacaagc tgcaaaacg tcaaatttg acacttctt 
      cgtgacatg tgcgtttga cattcccat gcatttttt 
      aaaaaagta tccaaaaat tgttaaatt tacatgttt 
      ggctacagt ttcattcgg cgcaggatg gttatatta 
      tcaggtaaa aacgaccaa aaaaatcct cagcaatgc 
      cttagacac atcaatttt atcaataat cgagccaac 
      agccgtgga aaaatccaa agatgatga ctttttcgt 
      cattgatat aactgggga cccgaaccg gcaagaaac 
      ctatttaca aaaatggac cgctttgaa gttttcatt 
      cgctaaaag attgcttgt tcttgcaag tagtgaaaa 
      tcggtttct tcggaaaag ggctgtgag acagttggc 
      taggattaa aaaattgga aagctggca atactcgca 
      actcgctga tatgattga tgccaggac tgcggtagg 
      gctggctag gacacccga tgttgagcc tagattatt 
      caacaaccc gcccttaag atttctcgg tcatacgca 
      cgacatgat tgtcgatgt gatgaaaga ttttttagt 
      caatttaca cgcgatgtc tcaacaccc ttgtatggg 
      ttatttcat ctctaaggc atttctcga cttgcatga 
      agttgagaa attcggtga tgacataac tccctacga 
      gttgaaaaa cttcattca aaccagtga agaactcct 
      accgacgtg tctagagtc acttttaca ccacgccag 
      tttagggaa atacgccaa agatattgc cgaactcaa 
      cggtcttcc acttttaat ggaaattca caaccggtt 
      caaagacaa cctttctgt caccgcaac tatttgtcg 
      gattatttc gtatctttc aatgcacag aaatctcct 
      tgacgaaat gtataaatc gggttgggt cgggctttt 
      ggcccagtg ggtcaaaca cgacgacgt ccgaatatc 
      aatccctgc atctaaagc acttgcaaa tttagactg 
      tgatgaaga ccctgttta cctacaaag actctaccc 
      tttgcatcc ttcaacccg tattgtaca aaccccgaa 
      agttgacgt cgtttttat ccatggttt actgggggg 
      tgtattttt tacgtggag gcagagaca acgcaacca 
      ggatccgtt ggggttcct tggtaagaa gggcgcccc 
      agaaagtct gacacagaa caactcaac ttgtatcaa 
      taaacaatg caccgaaaa gagaaacaa aatccaaag 
      aataagcga tcctcaaat gagggaatt tctggaaga 
      gtttgaaac gcacacagc ttatggttg cctttgcaa 
      cgattatga ctttgtttg ggaggacat accggtcaa 
      tacaaatga gactgccac agggccgta ctgttgttc 
      tggctttaa atacgtctg tatggaaaa aagtgagga 
      tgaatgtta caccaattg ctggcctag agactggtt 
      agcgcaaga ttgtgatcg tttgagaat cattggggt 
      aaattatga gacaaatct atcgttgtg gacacccat 
      atgtccagt agagaaagt caagacttt ggaagagcg 
      aagcgacga gttgattga acagttgga gagggttga 
      agttgggaa aagacccat cgtgtgggt gacacactc 
      aatgggtgg tttaatggt caagtgtct cctaaataa 
      agcttccga aaatggcaa taaacgaat cagagattt 
      gtacacaaa cactcgagg gataatttt ctatagcac 
      gcctcattt aggatcaag tgttgctag tttcagcca 
      ggcttcagc ccttgtgat ttggccaag tattgaagt 
      ccaggagtt acaaaaaca ttcgccaca attggtgca 
      aatacacca aaaattcct gagacttat caaaaatca 
      tccaatgaa aattgtgac ttttgtgga gaccaaacc 
      tacggtagt ttcagcaat gaaatttaa atttttagt 
      ggttgaacc cgactctgg caatccggg gtatgggga 
      atactatga gataccttt agaccattt gggaatttg 
      caaaccggc aagcagaca ttcgtttct ataccaaaa 
      agtgcttca catgatcac gtccatttt ggaggaaag 
      tgggccaga tattagcgc gttctctga tgataattt 
      cgataccaa agcgtggat aaacgatgt tttaaaaaa 
      tgcagagac ccaggacaa gaaagagaa ttatacaat 
      gtccctcgt tatgaagct ccaactatg cgtccaaca 
      agtgaacag tgcccttga agacaccag tcaacaaat 
      cctaatgtc actgccaaa aatcatcag agacaccaa 
      aaatctcaa ccaggaagc agcaatttt aaaggaaaa 
      aatggcagc agttcgtag cgaaatcgt aaaaattga 
      acaagacac tggaaagtc tataaacac aattgagaa 
      actggacac tattaagaa tgaactgaa tatggccaa 
      gcaggggct gcatgagag tgataattg gactatttt 
      agtcaacga cttggagga agtctttga ttccaaaaa 
      cattgaaaa catttccgc aaaaataat aggaatgca 
      gcggtcttt gcaactttt agtcaatgt tagtgatta 
      tgaagatcg taaattaca gttggaagg gctgaaaaa 
      tcggctgga agcaatcgc aagtccagc catagtcca 
      ggctttcac ttcttccaa cacagaaca atcgtcaat 
      gtttgtgcg aatatttaa atcaatggg acgcttacc 
      acaacttct aaagtacta ccacaagtg ccagaagga 
      cgttttatt gaaaaagtg gcgcaatca attggaaat 
      cgaacaaga cgagtctgt gatccaatg gatccacaa 
      tttttacgg cattatgct gtccaactg gcacacgca 
      acaaaaatg gttcaacca agtcttcac caaccaaaa 
      cagttgcga atcattcgt tgagattta caccgacgt 
      tctcacgtc tttagaccc gactctcaa cgaatgcat 
      tgacgccgc tttaaaaca agtcgaaga taaattaaa 
      ctttctctt cgaaatcaa acaaatcac ccaacagtt 
      cgccgacaa cttaacgga cgtcattaa ccaatcacc 
      gggcggcaa actagccca tgacaagtg cttgctgct 
      actacaagc agtctacaa ccacttaat cccatacac 
      tagcaaata cgcggctta tgaacaagc ccacctaat 
      gaaaaaact ctcggccat aaattgcat gaaagagga 
      gctttctga tacaatcca agcccttgg cttaagcat 
      ccctcaaat aatcgacat tgcacggga cgccaaaaa 
      acgctgcca gaaaataac cgaaaattg cggttactg 
      tggcttgtt aattgcact tcgggcgtt tctattaag 
      ctatgcgga ccaatatcg cgtcgcttt gcgacaaat 
      cgatcgaaa taaaagaca agaggagga ttggggcac 
      gtttcagtt gtgtctatc gttactgca gaacacggg 
      cgaagtcct ggtcaattt gcaacagct ggagaagga 
      cctgaccgg gactatact cgaaactaa tcaaattaa 
      agcccagtt tgagtacaa gtttttact attaagtgc 
      ctctgatcg aaaggaata cgaatcttt ggtcaggtg 
      tgtcactga agggacgca attgtcgct acttgacca 
      cgttaataa tgagtttag taaattgtg ctcggacat 
      acaccatac gacttatct tgtcatttt cgcaccgat 
      ttcgctaca tttggacgt ggtacaagt gccgaaaac 
      gtgggcaca attcgcaaa ttcaatgca caatgactt 
      gcccgatta tagtttcac gccacagga gtacatcac 
      acagatcgg tcagtactt gatgacgtt accgcaaca 
      cctggaacc attcctctt ccgagacaa cccgtcact 
      tagctgcgc cctgaaggc ggtggatca ggagtacag 
      cactgccgg agagacgga aggagccct agctcatgt 
      cttcctcac gtttatcgc caggaggac gtgtcaggg 
      attttgcga acgcatttt gtcaatttg tgaattgag 
      ccaagcggc cagccgaca attggccca cgatattgg 
      ttacttgga aaatgtctt gcaggattt ggggatttc 
      tatcagtta ggcagtacc ggatgtccg gctaggttt 
      gtagctgca gtccggcaa atgcgaaat ataacatcc 
      a
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