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Gene information
General information
TC001268 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13475573..13481979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001268
    iBeetle screen iB_06829

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001268 RA
      MKLFLVGCV FIAYFVHVL VANQKGCSF SVHGQSEKN 
      SPILLQNDT TLIVPNNGK VSLKRRDTV TLLCPDSKN 
      YLVGTQSNI TQAQCVQGG ILRVANQDL TFRDLQCKK 
      IIRGTVAKT TKKCGENKG RIYKIGYQI SSRNFLTLI 
      EVCYDPNSG TTLYTEHAL HGQDIKYAS KSNYRPAFS 
      PEASAVAAS VAYKQTFQK STFNKLMKS ALKAQEYIN 
      ENSFLSRGH LSPDADFLY AATQYTSYY YINAAPQWQ 
      TINAGNWKK IELLVRKLA DNLQETLTV ITGTYGVLT 
      LPDVNDNEV DVYLVSGSK LPVPKFFWK IIYAKHSRQ 
      AVVLVSLNN PFVKEIGKG DFLCSNVCS KVGWGSGSW 
      SNYERGFVY CCDYKQFVD KVETAPKLS VVGVLQGPK
    CDS sequence
    • >TC001268 RA
      atgaagctg ttcctagtc gggtgtgtt tttatcgcc 
      tatttcgtc cacgtactc gtagcgaac caaaaagga 
      tgctctttt tcggtgcat ggccaaagt gagaaaaat 
      tctcccatc ttgctgcag aacgacacg acattgatt 
      gtgcccaat aacggtaaa gtcagcttg aagcggaga 
      gacaccgtc acgctgctg tgtccagat tccaagaat 
      tatttagtt ggaactcaa tcaaacata acgcaggca 
      cagtgcgtg caaggaggc attttacgc gtggccaat 
      caggacctg actttccgc gatcttcag tgcaagaag 
      atcatcaga gggacggtg gccaagacc acgaagaaa 
      tgtggggag aataaggga cggatttac aaaattggc 
      taccaaatt tcctcgcga aacttctta acactaatc 
      gaagtttgc tatgatcct aactcagga accacccta 
      tacactgag cacgcactc cacggacaa gacatcaaa 
      tacgcttcc aaatcgaat taccgccca gcttttagt 
      cctgaagct tccgcggtt gcggcttcc gtcgcgtac 
      aaacagacg ttccagaaa tcaactttc aataaatta 
      atgaaatcg gcgctaaaa gcccaggaa tacatcaac 
      gaaaactcg tttctatcc cgtggacat ttatcgccc 
      gacgctgat tttctctac gccgccact caatacacc 
      tcctactat tacataaac gcggccccg caatggcaa 
      acaatcaat gccggaaac tggaaaaaa atcgagctt 
      ctagtgcgg aagctcgcc gataacctt caagaaacc 
      ctaaccgta atcacagga acctacggt gttttgact 
      cttccggac gttaacgac aacgaggtg gatgtatat 
      ctcgtttcc gggagcaaa ctaccggtg cctaaattt 
      ttctggaaa attatttat gccaaacac tcgagacag 
      gctgtagtt ttggtcagt ttgaacaat ccttttgtt 
      aaggaaatt gggaaagga gatttcttg tgttcgaat 
      gtttgcagt aaagttggg tggggttca ggatcatgg 
      tcaaactac gaacgcggt tttgtgtat tgttgtgat 
      tacaaacag tttgttgat aaagttgaa accgcaccg 
      aaactcagc gttgtgggc gtgctacaa gggccgaag 
      taa
    mRNA sequence
    • >TC001268 RA
      tgtgactct caaaattaa aaactactc cagttaaaa 
      acaactacg taatgatta cgtatgaag tgcaattgt 
      caatccaat taactagat aactggtgc tctcagcat 
      gattcatag ctgggcgat taagttatc gcgattgac 
      cacatcttt gcctaccga tggctaatt tgtacagtt 
      acaaaatga agctgttcc tagtcgggt gtgttttta 
      tcgcctatt tcgtccacg tactcgtag cgaaccaaa 
      aaggatgct ctttttcgg tgcatggcc aaagtgaga 
      aaaattctc ccatcttgc tgcagaacg acacgacat 
      tgattgtgc ccaataacg gtaaagtca gcttgaagc 
      ggagagaca ccgtcacgc tgctgtgtc cagattcca 
      agaattatt tagttggaa ctcaatcaa acataacgc 
      aggcacagt gcgtgcaag gaggcattt tacgcgtgg 
      ccaatcagg acctgactt tccgcgatc ttcagtgca 
      agaagatca tcagaggga cggtggcca agaccacga 
      agaaatgtg gggagaata agggacgga tttacaaaa 
      ttggctacc aaatttcct cgcgaaact tcttaacac 
      taatcgaag tttgctatg atcctaact caggaacca 
      ccctataca ctgagcacg cactccacg gacaagaca 
      tcaaatacg cttccaaat cgaattacc gcccagctt 
      ttagtcctg aagcttccg cggttgcgg cttccgtcg 
      cgtacaaac agacgttcc agaaatcaa ctttcaata 
      aattaatga aatcggcgc taaaagccc aggaataca 
      tcaacgaaa actcgtttc tatcccgtg gacatttat 
      cgcccgacg ctgattttc tctacgccg ccactcaat 
      acacctcct actattaca taaacgcgg ccccgcaat 
      ggcaaacaa tcaatgccg gaaactgga aaaaaatcg 
      agcttctag tgcggaagc tcgccgata accttcaag 
      aaaccctaa ccgtaatca caggaacct acggtgttt 
      tgactcttc cggacgtta acgacaacg aggtggatg 
      tatatctcg tttccggga gcaaactac cggtgccta 
      aatttttct ggaaaatta tttatgcca aacactcga 
      gacaggctg tagttttgg tcagtttga acaatcctt 
      ttgttaagg aaattggga aaggagatt tcttgtgtt 
      cgaatgttt gcagtaaag ttgggtggg gttcaggat 
      catggtcaa actacgaac gcggttttg tgtattgtt 
      gtgattaca aacagtttg ttgataaag ttgaaaccg 
      caccgaaac tcagcgttg tgggcgtgc tacaagggc 
      cgaagtaat tgcctcgtt tcttccgag ttttaatta 
      aacgaatgc cgatttcta caatgtatc attaagtaa 
      tttgtatta cgatacaaa gaaagaaaa ttcattgct 
      tgatacgag caagtagtt aaaggaatg tgtaaaggg 
      atgtttgaa ataaagcaa atataaaaa g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY