Status
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Gene information
General information
TC001255 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13258141..13261762
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001255
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06826

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001255 RA
      MVKDKTSES MDFDQAIHL TGYGKFHFE AFTACAICI 
      ISVGFQNGL SAYILPAAQ CELDLTSFQ LSLLNVCFL 
      VGGALSSFL WGVLADARG RRKILIITH LLNAAVTII 
      CVTNPSVFS IIICRFLNG FLIGAPGSI IFSYLAEFQ 
      PPKYRSAII CYSGIFFTS SWLLLPLLA WLILPIDIS 
      VTVGSVIKI TSWRVFLII LVIPEVVAG FWFMRLPES 
      PKFFLAKGD QRKALAVLR KMFVVNTGR PAKDFPVKS 
      LVREVRVAT FEDNLRCRG KTVKNLQEM FGQFKSLFR 
      PPLVFLTLL TTSIMFTNM FGTFGLGWW LPELFVRFE 
      RFQALNPNS TVTVKQLAL FNTIKPASC KPSFEAAVI 
      RSTIIMGVS SILTNAFSG WLAARVSLR TIPFGTMLV 
      GGTSASLIY WLTASWQNL IIASIFQSA MVTANMTIG 
      SVVVELFPT NVGAMAICL TMCAGRIGA MASNVIFGL 
      LMDQHCEIP IFVVAASVL VGAGLCYFI PSQKGHKGG 
      CADLEVAVI SSFDTKM
    CDS sequence
    • >TC001255 RA
      atggtcaaa gacaaaact tccgaatcg atggatttc 
      gaccaagcc atccacctg acaggttac ggcaaattc 
      cattttgag gccttcaca gcctgtgca atttgcata 
      atcagtgtc ggcttccag aacggcctc tcggcttac 
      attcttcca gcggctcag tgcgagctg gacctcacg 
      tccttccaa ctgagtctt ttgaacgtg tgtttcctg 
      gtggggggc gccctgagc agcttcctg tggggcgtt 
      ttggccgat gcccgaggc cgcaggaaa atcctgatc 
      attacccat ctcctgaac gccgcagtc acgataatt 
      tgtgtcaca aatccctca gtgttcagt ataataatt 
      tgcagattt ttgaacggg tttctaatc ggggcccct 
      gggagcata attttttcg tatttagct gaattccaa 
      ccccccaag taccgctcg gctattatt tgctactct 
      gggattttt ttcacctcg tcttggctt cttttaccc 
      ctcctggcc tggcttatc ctgccaatt gatatctcg 
      gtaactgtc ggatcggtc attaaaatc acttcgtgg 
      agagtattt ttgataatt ttggtgata ccggaggta 
      gtggcgggt ttttggttc atgcgtttg cccgaaagt 
      cccaaattt tttctcgct aaaggggac caaagaaaa 
      gctttggct gttttgagg aaaatgttc gtggtcaat 
      accggaagg ccggcgaag gattttcca gtcaagagt 
      ttagttcgg gaagtgaga gttgcaacg tttgaggat 
      aatttgagg tgtaggggg aagacggtg aaaaatttg 
      caagagatg tttgggcag tttaagagt ctttttagg 
      cctccgctc gttttttta actctgctc acgactagc 
      atcatgttt acgaatatg tttggcact tttggcctc 
      ggctggtgg cttccggag ctcttcgtc cggttcgag 
      cgctttcag gcgctcaac cccaacagt accgtcact 
      gtcaagcaa ctggccctt ttcaacacc atcaagccg 
      gcgtcctgc aagccatcg ttcgaagct gcggttatt 
      aggagtacc atcatcatg ggcgtttct tccattttg 
      accaacgcc ttttcggga tggttggcg gcgagggtg 
      tccctcagg accatcccg ttcggtacc atgctggtg 
      ggtggtacc agcgctagt cttatctac tggttgacc 
      gcctcgtgg cagaatcta atcatcgct tcgattttt 
      caatcggcg atggtcaca gctaatatg acaattggg 
      agtgtggtg gtggagctt tttccgaca aatgtgggt 
      gctatggct atttgtctg acaatgtgt gctgggaga 
      attggggcg atggctagt aatgtaatt tttggactg 
      ttaatggac caacattgc gaaatcccg atttttgtg 
      gtggctgct agtgtttta gtcggcgcg ggactttgt 
      tactttatc ccgtcgcaa aaggggcat aaggggggc 
      tgtgccgat cttgaagtt gccgtcatt tcaagtttt 
      gatacgaaa atgtga
    mRNA sequence
    • >TC001255 RA
      gctcaaata tttaccccg ctatgaaaa cacaattct 
      tgaagaaaa atgtgtcta gtagcctcg caacccttg 
      cccccgtta cacacttcc aataccgct tacttgaca 
      aaaaattgc ccaacaatt atgaaacta aaacaatga 
      accgatcaa tggtcacat tcaacccat catggtcaa 
      agacaaaac ttccgaatc gatggattt cgaccaagc 
      catccacct gacaggtta cggcaaatt ccattttga 
      ggccttcac agcctgtgc aatttgcat aatcagtgt 
      cggcttcca gaacggcct ctcggctta cattcttcc 
      agcggctca gtgcgagct ggacctcac gtccttcca 
      actgagtct tttgaacgt gtgtttcct ggtgggggg 
      cgccctgag cagcttcct gtggggcgt tttggccga 
      tgcccgagg ccgcaggaa aatcctgat cattaccca 
      tctcctgaa cgccgcagt cacgataat ttgtgtcac 
      aaatccctc agtgttcag tataataat ttgcagatt 
      tttgaacgg gtttctaat cggggcccc tgggagcat 
      aattttttc gtatttagc tgaattcca accccccaa 
      gtaccgctc ggctattat ttgctactc tgggatttt 
      tttcacctc gtcttggct tcttttacc cctcctggc 
      ctggcttat cctgccaat tgatatctc ggtaactgt 
      cggatcggt cattaaaat cacttcgtg gagagtatt 
      tttgataat tttggtgat accggaggt agtggcggg 
      tttttggtt catgcgttt gcccgaaag tcccaaatt 
      ttttctcgc taaagggga ccaaagaaa agctttggc 
      tgttttgag gaaaatgtt cgtggtcaa taccggaag 
      gccggcgaa ggattttcc agtcaagag tttagttcg 
      ggaagtgag agttgcaac gtttgagga taatttgag 
      gtgtagggg gaagacggt gaaaaattt gcaagagat 
      gtttgggca gtttaagag tctttttag gcctccgct 
      cgttttttt aactctgct cacgactag catcatgtt 
      tacgaatat gtttggcac ttttggcct cggctggtg 
      gcttccgga gctcttcgt ccggttcga gcgctttca 
      ggcgctcaa ccccaacag taccgtcac tgtcaagca 
      actggccct tttcaacac catcaagcc ggcgtcctg 
      caagccatc gttcgaagc tgcggttat taggagtac 
      catcatcat gggcgtttc ttccatttt gaccaacgc 
      cttttcggg atggttggc ggcgagggt gtccctcag 
      gaccatccc gttcggtac catgctggt gggtggtac 
      cagcgctag tcttatcta ctggttgac cgcctcgtg 
      gcagaatct aatcatcgc ttcgatttt tcaatcggc 
      gatggtcac agctaatat gacaattgg gagtgtggt 
      ggtggagct ttttccgac aaatgtggg tgctatggc 
      tatttgtct gacaatgtg tgctgggag aattggggc 
      gatggctag taatgtaat ttttggact gttaatgga 
      ccaacattg cgaaatccc gatttttgt ggtggctgc 
      tagtgtttt agtcggcgc gggactttg ttactttat 
      cccgtcgca aaaggggca taagggggg ctgtgccga 
      tcttgaagt tgccgtcat ttcaagttt tgatacgaa 
      aatgtgatt cttagagtg actttttgt tagataatt 
      catatcttt tatataatt gttgtgtga taaattatt 
      tgttgctac ttacttatt ggttttgtg gacgttagt 
      aacagaatc atttcgttg caaaatgaa ggaaaacta 
      cgagattgt ttcaaactg cctttatta ttcatcgat 
      aagtgacta acagtaata ttttgatgg aatttccca 
      attaataca tttttgcca ttcgtgtta cataattat 
      aaggaacgt caggaaatt tctctttga tgagtgttg 
      gacgaggtg ttggagtgg ctaacacct gctgctgca 
      gccctagtt gggtggcag atggtgaat gtcatgaag 
      gggtaaata aattttatc gatccctca tcgaaaatg 
      tccctgtgt ttctgctat cctgataaa agtaacacc 
      cggcgtcgt gacgctccg gaaccggaa cccggcacg 
      acgccggcg cttaaattt ttttttcat tactgacca 
      aataaagag tgtacttta ta
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