Status
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Gene information
General information
TC001250 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13221561..13225575
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001250
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06823

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late posteriorized - Number of animals showing the phenotype: 3
    tropharium structure irregular - Number of animals showing the phenotype: 3

    vitellogenic egg chamber early potentiallydegenerated - Number of animals showing the phenotype: 3

    egg chamber number decreased - Number of animals showing the phenotype: 3
    previtellogenic egg chamber potentially present - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001250 RA
      MTSRAKNVT TRIEVDIEK NREEGNWLK VIELAEQLK 
      EKSPELVCL GDFLIGEGK LENYLEEWP PVEANIDRA 
      KIGLIDAKR YLTLVINDA GIKAGVAMD AHLLLGKLH 
      YASGQYSVA LKHFKSAEL HNLSQKKLP LRSLRIVAE 
      SYAVNGLCL QKDTTSTSK FKKAEKEEE MSKCFGLAS 
      DLSLLYMQK LEKEPTGTT GTTGTHSPQ PPPSHKSLG 
      IILEQAMQE GPTLSLQLG KPEEALERY RAFLCAVET 
      HGGHAIRLK FMCQMAELI LQVLLGDKY APPLNSLPK 
      PAIWKPKYY ASLNQFVPR NECEETLLL LLIAESMAV 
      RNAVLSQSP EFKEVRLSA YQDATRVYD LLTVATVRW 
      GQVTLLQES LERSMKFSF EEPHLWKQH GLSLLATGR 
      YVHALAVLK EVIRLEPNN ASNCLLAAK ICYEHLNLA 
      VEGTNFSIE AKKKDMQCN SGLIGRCYL YIGIGYYLQ 
      AESCLLKKD KEELLNRAL ENFKNAVEI EPNDHLCHY 
      YLGLQLAVL GKISEAQNH VRISLDLRS ESSSTLHLL 
      VLLLTAERE HKNALTIVE NALEEYPDC LNLMYVKAH 
      LELHEEGGE KALVTAKQM LELWKNLYE GQLNSDVPE 
      CDRKSDTRS VFQLYTSEM SDRDSSSLQ LHNTAASRV 
      EQALSEVAS SLSSFSPRP GPQRAWMLQ VEVWLLLAE 
      LYLAMDQPA DVQQCIQEA TQIYPLSHH IMHMKGLLH 
      MHKQEWPEA KLCFQNAVA INPQHVKSL QELGLVYHY 
      LGLQGLAET TFREAAKID PKNHITWYN LGKVLEALG 
      EYEKASNAM ATALMEEKN NPILPFNSV PLCFE
    CDS sequence
    • >TC001250 RA
      atgacaagt agagcaaaa aatgtaaca actcgaatt 
      gaagttgat attgagaaa aacagggaa gaaggcaac 
      tggttgaag gtcatagaa ttagccgaa caactcaaa 
      gaaaagtcg ccagaactt gtgtgtctg ggggacttt 
      ttaataggc gaaggcaaa ttagagaat tacttggag 
      gaatggccc ccagttgag gccaacatt gacagggcc 
      aaaattggc ctaattgac gcaaaaaga tacctaact 
      ttggttatc aacgatgcg ggaattaag gcaggcgtt 
      gcaatggac gcccacctc ctcttgggt aagctccac 
      tacgcctct ggtcagtac tctgttgcc cttaaacac 
      ttcaaatcg gccgaactt cacaacctc tcgcagaaa 
      aaactccca ctccgcagt ttacgcatc gtcgccgaa 
      tcgtacgcc gtcaacggc ctgtgcctc cagaaagac 
      acgacctcg actagcaaa ttcaaaaaa gccgaaaaa 
      gaggaagaa atgtccaaa tgtttcggt ctcgcctcc 
      gatttgagt ctcctctac atgcaaaaa ctcgaaaaa 
      gagcccacc gggacaacg ggcaccaca ggcacacac 
      tccccccag ccgccaccc tcccacaaa tccctcggg 
      atcatcctg gagcaagcc atgcaggaa ggacccacc 
      ctttccttg cagctgggc aagcctgag gaggctctg 
      gagcggtac agggccttc ttgtgtgcg gttgaaacg 
      cacgggggg cacgccatt cgcttgaaa ttcatgtgt 
      cagatggca gagttgatt ttgcaggtg ttgttgggg 
      gataaatac gcgccaccg ctcaattcg ctacctaaa 
      cctgccatt tggaaacca aagtattac gcgtctttg 
      aatcagttt gttccgagg aatgagtgc gaggagacg 
      ctcttgttg ctgctgatt gcggaatcg atggctgtt 
      aggaatgcg gttttgtcg caatcgcca gagtttaag 
      gaagttagg ctgagtgcg tatcaagac gccaccagg 
      gtttatgac ttgttgacg gtggccacg gttaggtgg 
      gggcaagtg acgctgctt caggagtct ttggaacgc 
      tccatgaag ttttccttc gaggagccc cacttgtgg 
      aaacaacac ggtcttagc cttttggcc accggccgc 
      tacgtgcac gctttagcc gttctcaaa gaagtcatc 
      cgtcttgaa cctaacaat gctagtaac tgtttactg 
      gctgcaaaa atctgctac gaacactta aacctagcc 
      gtagaaggc accaatttc agtatagag gctaaaaag 
      aaggacatg caatgtaat tcgggtttg atcggccgt 
      tgttacctt tacataggc ataggttat tacttacaa 
      gccgaatcc tgtctcctc aaaaaagac aaggaggaa 
      ttattgaat cgggcttta gaaaatttc aaaaacgcg 
      gtcgagatt gaacccaac gaccacttg tgtcactat 
      tatctcggg cttcaattg gcggtcttg ggtaagatt 
      agtgaagcg caaaaccac gtcaggata tctctagat 
      ttacgatcg gaaagttcg tccacgtta catctacta 
      gtgttgctt ttaactgcg gaaagggag cataaaaac 
      gccctaact attgtggaa aacgcccta gaggagtac 
      cccgactgt cttaattta atgtacgtt aaggcccac 
      ctagaacta cacgaagaa gggggcgag aaggctcta 
      gtaacagcc aaacaaatg ctcgagctg tggaaaaac 
      ctctacgaa ggtcaatta aattccgac gttccggaa 
      tgcgaccga aaaagcgat acacgttca gtcttccag 
      ttatacacc tctgaaatg tccgaccgg gattcgagt 
      tcattacag ctgcacaac accgcggct tcgagagtc 
      gagcaagcc ctaagtgaa gtcgcgagt tcgttgagt 
      agttttagc ccgcggcct ggcccgcag agggcgtgg 
      atgttgcaa gttgaggta tggttactg ttggctgaa 
      ctgtactta gcgatggac caaccggcc gatgtgcaa 
      caatgtata caagaagcc acgcaaata tacccctta 
      agtcaccat ataatgcat atgaaaggg ctgctgcac 
      atgcataag caggagtgg cccgaggcg aaactgtgc 
      ttccaaaat gctgtcgcg atcaatcca cagcatgtc 
      aagtcgctg caagagctg gggttggtt taccactat 
      ctggggttg caagggttg gccgagacc acgttcagg 
      gaggcggcg aaaatcgac cccaaaaat cacatcaca 
      tggtataat ttaggtaag gtgttggag gcgctaggc 
      gagtacgag aaggcgagc aatgcgatg gcgacggcc 
      ctcatggag gaaaaaaat aatccgata cttcccttc 
      aattcagtt cctctttgt tttgaataa
    mRNA sequence
    • >TC001250 RA
      tgttttgac aactagaaa acgtcaatt ccattccca 
      tatgactga tgtaggtta tgttgtgac aagtgtgat 
      ttatagttt attttttgt tatttatgg aaacaggag 
      tttaaaaaa tgacaagta gagcaaaaa atgtaacaa 
      ctcgaattg aagttgata ttgagaaaa acagggaag 
      aaggcaact ggttgaagg tcatagaat tagccgaac 
      aactcaaag aaaagtcgc cagaacttg tgtgtctgg 
      gggactttt taataggcg aaggcaaat tagagaatt 
      acttggagg aatggcccc cagttgagg ccaacattg 
      acagggcca aaattggcc taattgacg caaaaagat 
      acctaactt tggttatca acgatgcgg gaattaagg 
      caggcgttg caatggacg cccacctcc tcttgggta 
      agctccact acgcctctg gtcagtact ctgttgccc 
      ttaaacact tcaaatcgg ccgaacttc acaacctct 
      cgcagaaaa aactcccac tccgcagtt tacgcatcg 
      tcgccgaat cgtacgccg tcaacggcc tgtgcctcc 
      agaaagaca cgacctcga ctagcaaat tcaaaaaag 
      ccgaaaaag aggaagaaa tgtccaaat gtttcggtc 
      tcgcctccg atttgagtc tcctctaca tgcaaaaac 
      tcgaaaaag agcccaccg ggacaacgg gcaccacag 
      gcacacact ccccccagc cgccaccct cccacaaat 
      ccctcggga tcatcctgg agcaagcca tgcaggaag 
      gacccaccc tttccttgc agctgggca agcctgagg 
      aggctctgg agcggtaca gggccttct tgtgtgcgg 
      ttgaaacgc acggggggc acgccattc gcttgaaat 
      tcatgtgtc agatggcag agttgattt tgcaggtgt 
      tgttggggg ataaatacg cgccaccgc tcaattcgc 
      tacctaaac ctgccattt ggaaaccaa agtattacg 
      cgtctttga atcagtttg ttccgagga atgagtgcg 
      aggagacgc tcttgttgc tgctgattg cggaatcga 
      tggctgtta ggaatgcgg ttttgtcgc aatcgccag 
      agtttaagg aagttaggc tgagtgcgt atcaagacg 
      ccaccaggg tttatgact tgttgacgg tggccacgg 
      ttaggtggg ggcaagtga cgctgcttc aggagtctt 
      tggaacgct ccatgaagt tttccttcg aggagcccc 
      acttgtgga aacaacacg gtcttagcc ttttggcca 
      ccggccgct acgtgcacg ctttagccg ttctcaaag 
      aagtcatcc gtcttgaac ctaacaatg ctagtaact 
      gtttactgg ctgcaaaaa tctgctacg aacacttaa 
      acctagccg tagaaggca ccaatttca gtatagagg 
      ctaaaaaga aggacatgc aatgtaatt cgggtttga 
      tcggccgtt gttaccttt acataggca taggttatt 
      acttacaag ccgaatcct gtctcctca aaaaagaca 
      aggaggaat tattgaatc gggctttag aaaatttca 
      aaaacgcgg tcgagattg aacccaacg accacttgt 
      gtcactatt atctcgggc ttcaattgg cggtcttgg 
      gtaagatta gtgaagcgc aaaaccacg tcaggatat 
      ctctagatt tacgatcgg aaagttcgt ccacgttac 
      atctactag tgttgcttt taactgcgg aaagggagc 
      ataaaaacg ccctaacta ttgtggaaa acgccctag 
      aggagtacc ccgactgtc ttaatttaa tgtacgtta 
      aggcccacc tagaactac acgaagaag ggggcgaga 
      aggctctag taacagcca aacaaatgc tcgagctgt 
      ggaaaaacc tctacgaag gtcaattaa attccgacg 
      ttccggaat gcgaccgaa aaagcgata cacgttcag 
      tcttccagt tatacacct ctgaaatgt ccgaccggg 
      attcgagtt cattacagc tgcacaaca ccgcggctt 
      cgagagtcg agcaagccc taagtgaag tcgcgagtt 
      cgttgagta gttttagcc cgcggcctg gcccgcaga 
      gggcgtgga tgttgcaag ttgaggtat ggttactgt 
      tggctgaac tgtacttag cgatggacc aaccggccg 
      atgtgcaac aatgtatac aagaagcca cgcaaatat 
      accccttaa gtcaccata taatgcata tgaaagggc 
      tgctgcaca tgcataagc aggagtggc ccgaggcga 
      aactgtgct tccaaaatg ctgtcgcga tcaatccac 
      agcatgtca agtcgctgc aagagctgg ggttggttt 
      accactatc tggggttgc aagggttgg ccgagacca 
      cgttcaggg aggcggcga aaatcgacc ccaaaaatc 
      acatcacat ggtataatt taggtaagg tgttggagg 
      cgctaggcg agtacgaga aggcgagca atgcgatgg 
      cgacggccc tcatggagg aaaaaaata atccgatac 
      ttcccttca attcagttc ctctttgtt ttgaataat 
      gcgttgtta cttgttact ttattgtta cgtaaataa 
      tggtgattg tacttaact gtgttatag gttaatttg 
      tacaatttt agtcgttga tgtttgtgt atgtcataa 
      ttaatgtga tagaagagc gatacagta atttgagcg 
      agtttcaag tttccgatc tgggtttta aggttgttg 
      ctagtttgc gtaattaaa ggtagaaac gagtggtgg 
      cgctacgac tcacctagt cataatatt tactgaggc 
      aatacttaa aattccgat tggggaaca atataccat 
      aaaaattgt tttaaattg taaaaatta ctcaactga 
      tggaaagag atattatat cagattaaa acaataata 
      ttgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY