Status
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Gene information
General information
TC000313 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13141688..13144070
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000313
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000313 RA
      MGKFKRYYD KAGDESTKE AVVYNVDPK PGPWGRRQA 
      DKRIKKNKK IVVKIPNPD PKFKGKVPI PKKLLRKHS 
      RGKGISPKG VRTNTHQKR LEKKEKVIH FATKQAARA 
      EVLLTEDAG FLEADAGET TTQFTQKQI AESVDITAA 
      TKSFELKLA DFGPYRMKY TRNGRHLLI GGKKGHVAA 
      FDWVTKKLH CEMNVMESV HDISWLHIE TMFAVAQKE 
      WVYIYDNQG IELHCVKRL NRVTRMEFL PYHFLLASC 
      SDSGYLSWL DISIGQLVS QFNTNLGRL TMLSQNPWN 
      AVLCVGHAK GVVSMWSPN SKTPLAKML CHKAPLTAL 
      HVDPKGQFL ATAASNREL KIWDVRKLS GPLQEYKLI 
      TAANNLNFS QKNMLALGM GNVVEVYRD CCMKPAKRP 
      YLRHRFVTS VGNLNFCPY EDVLGVATA TSVASLIVP 
      GAGEANFDA LEANPFQSK SQRREAEVK SLLEKIQPE 
      LITLDPTSI VEVDLPTLK DKVEAKQKL LHVKPPRIN 
      FTPRNKAKG KGGSVKVAK TKKIVKEQA KKEFIKNTK 
      DLAPKFGSK AEKEQGVLD RFLPKKKQI IANK
    CDS sequence
    • >TC000313 RA
      atgggaaag ttcaaaaga tattacgat aaagcgggt 
      gacgaatct acgaaagaa gcagtcgtt tataatgtg 
      gatcctaag cctgggcct tgggggcgc agacaggcc 
      gataaacgc ataaaaaag aacaaaaaa atagttgtg 
      aaaatccca aatccggac cctaagttt aagggaaaa 
      gtgccaatt ccgaagaaa ctcttacga aagcacagc 
      agaggtaag ggcattagt ccgaaggga gttaggaca 
      aacacgcac cagaaacgg cttgaaaag aaggaaaaa 
      gttatacat ttcgcaacg aagcaagca gcccgagct 
      gaggtcctt ttaaccgaa gatgccggt tttctcgaa 
      gcggacgct ggcgagacc acaacgcaa tttacgcaa 
      aaacaaatc gctgaaagt gtcgatata acagcggcc 
      accaaaagt tttgaatta aaattggcc gacttcggg 
      ccttatcgc atgaaatat actagaaat gggcgccat 
      ttgttaatt gggggtaaa aagggccat gtggccgca 
      tttgattgg gttacaaaa aagttgcat tgcgaaatg 
      aacgttatg gagtcggtt catgacatc agctggttg 
      cacattgaa acgatgttt gctgtggcc cagaaagag 
      tgggtctac atctacgat aaccaaggg attgaactg 
      cactgtgtg aaaagacta aaccgagtc acaagaatg 
      gagtttttg ccttatcat ttcttgcta gcttcttgt 
      agtgatagt ggttatttg agttggttg gatatttca 
      atagggcag ttggtgtcc cagtttaac acaaatcta 
      gggagactg acaatgttg agtcagaac ccttggaac 
      gctgtttta tgtgtgggg catgccaaa ggtgtggta 
      tccatgtgg tccccaaac tccaaaacc cctttagct 
      aaaatgctg tgtcataaa gccccactt accgccctc 
      catgtggac cctaaagga cagtttctt gcaactgcc 
      gcaagtaac agggaactt aaaatctgg gacgtgcga 
      aaactttcg ggacctttg caagaatac aaactaatc 
      accgccgca aacaattta aacttctcg cagaaaaac 
      atgttggct ttagggatg ggaaacgtg gttgaggtt 
      tacagagac tgttgcatg aagccggca aaacggccg 
      tacctccgc caccggttt gtaaccagt gtcggcaac 
      ttaaatttt tgcccctat gaagacgtc ttaggggtg 
      gccacagcc accagtgtg gcaagtctc atagtccct 
      ggggcaggc gaagccaac tttgatgcc ctggaagct 
      aacccgttc cagagcaaa tcgcaaaga cgcgaggct 
      gaagtcaaa tcgctcctt gaaaaaatt caacctgaa 
      cttataact ctagacccg acttcaata gttgaagtg 
      gatttgccg acactcaag gataaagtc gaagcgaaa 
      caaaaattg ctccacgtc aagccgcct aggattaat 
      ttcacgcca cggaataaa gcgaaaggc aaaggcggc 
      tctgtgaaa gtcgccaag actaagaaa attgttaaa 
      gaacaggcc aagaaggag tttatcaaa aatacgaag 
      gacttggcc ccaaagttt ggctccaag gcggaaaag 
      gagcaagga gtgttagat aggtttttg ccaaaaaaa 
      aaacaaata attgcaaat aaatga
    mRNA sequence
    • >TC000313 RA
      ttggtggca ctggtattt atgtgcaca catgttttc 
      ctaactcca atcgtgcaa tttaatttt tgataaaaa 
      aatgggaaa gttcaaaag atattacga taaagcggg 
      tgacgaatc tacgaaaga agcagtcgt ttataatgt 
      ggatcctaa gcctgggcc ttgggggcg cagacaggc 
      cgataaacg cataaaaaa gaacaaaaa aatagttgt 
      gaaaatccc aaatccgga ccctaagtt taagggaaa 
      agtgccaat tccgaagaa actcttacg aaagcacag 
      cagaggtaa gggcattag tccgaaggg agttaggac 
      aaacacgca ccagaaacg gcttgaaaa gaaggaaaa 
      agttataca tttcgcaac gaagcaagc agcccgagc 
      tgaggtcct tttaaccga agatgccgg ttttctcga 
      agcggacgc tggcgagac cacaacgca atttacgca 
      aaaacaaat cgctgaaag tgtcgatat aacagcggc 
      caccaaaag ttttgaatt aaaattggc cgacttcgg 
      gccttatcg catgaaata tactagaaa tgggcgcca 
      tttgttaat tgggggtaa aaagggcca tgtggccgc 
      atttgattg ggttacaaa aaagttgca ttgcgaaat 
      gaacgttat ggagtcggt tcatgacat cagctggtt 
      gcacattga aacgatgtt tgctgtggc ccagaaaga 
      gtgggtcta catctacga taaccaagg gattgaact 
      gcactgtgt gaaaagact aaaccgagt cacaagaat 
      ggagttttt gccttatca tttcttgct agcttcttg 
      tagtgatag tggttattt gagttggtt ggatatttc 
      aatagggca gttggtgtc ccagtttaa cacaaatct 
      agggagact gacaatgtt gagtcagaa cccttggaa 
      cgctgtttt atgtgtggg gcatgccaa aggtgtggt 
      atccatgtg gtccccaaa ctccaaaac ccctttagc 
      taaaatgct gtgtcataa agccccact taccgccct 
      ccatgtgga ccctaaagg acagtttct tgcaactgc 
      cgcaagtaa cagggaact taaaatctg ggacgtgcg 
      aaaactttc gggaccttt gcaagaata caaactaat 
      caccgccgc aaacaattt aaacttctc gcagaaaaa 
      catgttggc tttagggat gggaaacgt ggttgaggt 
      ttacagaga ctgttgcat gaagccggc aaaacggcc 
      gtacctccg ccaccggtt tgtaaccag tgtcggcaa 
      cttaaattt ttgccccta tgaagacgt cttaggggt 
      ggccacagc caccagtgt ggcaagtct catagtccc 
      tggggcagg cgaagccaa ctttgatgc cctggaagc 
      taacccgtt ccagagcaa atcgcaaag acgcgaggc 
      tgaagtcaa atcgctcct tgaaaaaat tcaacctga 
      acttataac tctagaccc gacttcaat agttgaagt 
      ggatttgcc gacactcaa ggataaagt cgaagcgaa 
      acaaaaatt gctccacgt caagccgcc taggattaa 
      tttcacgcc acggaataa agcgaaagg caaaggcgg 
      ctctgtgaa agtcgccaa gactaagaa aattgttaa 
      agaacaggc caagaagga gtttatcaa aaatacgaa 
      ggacttggc cccaaagtt tggctccaa ggcggaaaa 
      ggagcaagg agtgttaga taggttttt gccaaaaaa 
      aaaacaaat aattgcaaa taaatgaat ttattttgt 
      aagaagtgg tattatttc atcaagtaa cctgtccct 
      gtttcccgc gctcaccta ataacaatt gattttaca 
      aaaaaatac acgggaaaa tattcttac ggtaattaa 
      acgactggt gttgctgtt ttttatttt ttggaccaa 
      cggcagtga tgccttcag aggcactgt tgcaacaac 
      tcgaatgct cttgtcagt aattactct ctcaacgtt 
      tgcttgaaa cgttttact aaacatttc cattttccc 
      gatctcatc tatgataat aatcgattt cacctaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY