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Gene information
General information
TC000350 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12716193..12717493
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000350
    iBeetle screen iB_06818

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen cuticle structure irregular -

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000350 RA
      MASFSVINL QGYEAIIHE KLFANIKQL TYVYPPTQD 
      FHEVFREDM FKKNNKAAF QQVTNYLFN VLSPELTRE 
      KLKSWPPYD TKGEREFRQ EVVAFVDYL NENYKAANI 
      PKLMPSLFV SPGGIKVAK FMFKLSTFV LHEHLKKNM 
      KEGKLLSPI RPCKNPAVN AIIIENLNR TTSVIKKRT 
      SETATNFHK FSAEAFAEA QRIVEQVER IKRQEMAEV 
      TKALDEEKK KNLESIDLE KLQGKMAQM ENIKSMCER 
      ANELLDVIM NDNQLELDK ENTPDLDLL QLFNDLNEF 
      LEKERFQIP TLLQEDMKD MVQRLSELT ENYKQLIIN 
      MEHDKKELE KLAENSVI
    CDS sequence
    • >TC000350 RA
      atggcgtca ttctctgtg ataaatttg caaggttac 
      gaagccata atccacgag aaacttttc gcaaacatt 
      aagcaatta acttatgtt tatccacca acccaagac 
      ttccatgag gttttcagg gaagatatg tttaaaaaa 
      aataacaag gcagccttc caacaagtc accaactac 
      cttttcaat gtcctaagt cctgagttg accagagaa 
      aagctcaaa tcctggccc ccatacgac accaaagga 
      gagcgagag tttagacaa gaggtggtg gcatttgtt 
      gattacttg aatgaaaat tacaaagcg gcgaacatt 
      cctaagcta atgccatct ctttttgtt tcacctggt 
      ggcataaag gtggccaaa ttcatgttt aaattgtcg 
      acgttcgtc ttgcatgag catttaaaa aagaacatg 
      aaagagggc aaattattg tctcccatc agaccttgt 
      aaaaatcca gccgttaat gcaataatt atagaaaac 
      ttgaataga acaacgtca gttataaaa aaaagaacg 
      tcagaaact gccaccaac ttccacaaa ttctcggca 
      gaggcattt gcagaagcc cagcgaatt gtggagcaa 
      gtggagagg attaaaaga caggaaatg gccgaagtc 
      accaaggcg ttagacgaa gagaaaaag aaaaatctc 
      gaatcaatt gatttggaa aagttgcaa ggaaaaatg 
      gcacaaatg gaaaatatt aaaagcatg tgtgagaga 
      gcaaacgag ctcctggat gtcataatg aatgataat 
      caactggaa cttgataaa gaaaatact cccgacttg 
      gatttgctg cagttgttc aatgattta aatgagttc 
      cttgaaaag gaacgtttc caaatacca actttgtta 
      caggaagat atgaaagat atggttcaa cgcttgtca 
      gagctcaca gaaaactac aaacaactt atcattaat 
      atggagcac gacaagaag gaactcgaa aaattggca 
      gaaaattct gttatctaa
    mRNA sequence
    • >TC000350 RA
      attaaaata aaaaaacag ttaaaaaac gtctgtttt 
      taataaaaa aatcgtccg tctggtgat ttatttttc 
      tgacgttgg tcgtggtca attaaagtt actggtctt 
      tagtttaaa aaagtcgtg tttttgaag cttatttgt 
      tctttcaca gaatggcgt cattctctg tgataaatt 
      tgcaaggtt acgaagcca taatccacg agaaacttt 
      tcgcaaaca ttaagcaat taacttatg tttatccac 
      caacccaag acttccatg aggttttca gggaagata 
      tgtttaaaa aaaataaca aggcagcct tccaacaag 
      tcaccaact accttttca atgtcctaa gtcctgagt 
      tgaccagag aaaagctca aatcctggc ccccatacg 
      acaccaaag gagagcgag agtttagac aagaggtgg 
      tggcatttg ttgattact tgaatgaaa attacaaag 
      cggcgaaca ttcctaagc taatgccat ctctttttg 
      tttcacctg gtggcataa aggtggcca aattcatgt 
      ttaaattgt cgacgttcg tcttgcatg agcatttaa 
      aaaagaaca tgaaagagg gcaaattat tgtctccca 
      tcagacctt gtaaaaatc cagccgtta atgcaataa 
      ttatagaaa acttgaata gaacaacgt cagttataa 
      aaaaaagaa cgtcagaaa ctgccacca acttccaca 
      aattctcgg cagaggcat ttgcagaag cccagcgaa 
      ttgtggagc aagtggaga ggattaaaa gacaggaaa 
      tggccgaag tcaccaagg cgttagacg aagagaaaa 
      agaaaaatc tcgaatcaa ttgatttgg aaaagttgc 
      aaggaaaaa tggcacaaa tggaaaata ttaaaagca 
      tgtgtgaga gagcaaacg agctcctgg atgtcataa 
      tgaatgata atcaactgg aacttgata aagaaaata 
      ctcccgact tggatttgc tgcagttgt tcaatgatt 
      taaatgagt tccttgaaa aggaacgtt tccaaatac 
      caactttgt tacaggaag atatgaaag atatggttc 
      aacgcttgt cagagctca cagaaaact acaaacaac 
      ttatcatta atatggagc acgacaaga aggaactcg 
      aaaaattgg cagaaaatt ctgttatct aataaagat 
      tgaaattac tttacattt tttgtacca taataataa 
      aaataaatt ttttgtatt acc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY