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Gene information
General information
TC001197 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12489647..12492431
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001197
    iBeetle screen iB_06817

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001197 RA
      MGNETGRDM ETESIKSEN SSRSRRSRG HRDRNNYRQ 
HSHRSTRSS KSRKNDEMA PFQTAVTLT PDNRDGQEV 
IEVQILPQD DNWGENTTA ITGNTSEQS QSTEDVSNW 
PGEPDTGLS FVCQRYMGT VLTYILSIV AFLSPLAMV 
VLPKLGFFP ALSSRLGLA DSAKLLACG AECKGQLVS 
LAFKMAILA VGAWAVFLR KPNATLPRF FLFRAAVLA 
FVLVCIFAY WLFYIVQVT ETARAATTP EETVDYEAL 
VAYALSLTD TLLFIHYLA VVLMEIRHQ QPMYYIKVV 
RSPDGASRA YAIGQLSIQ RAAVWVLEK YYTEFPIYN 
PYLERIPVS KSRKNSSSF KFYEVDGVN NSSLQQSQS 
RAMLAAHAR RRDSSHNER FYEEHEYER RVKKRRARL 
ITAAEEAFT HIKRLHNDQ VPAIPMDPH EAAQAIFPS 
MARALQKYL RVTRQQPRH SVESILTHL ANCLSNDLT 
PKAFLEQYL VSEPVLQND KEVKDVQTW ALICEELLT 
RPIGDNTSF QLRQNDVSL LCTIKKIPH FNITEEVVD 
PKSNKFVLR LNSETSV
    CDS sequence
    • >TC001197 RA
      atgggcaac gaaactgga cgagatatg gagactgaa 
tccataaaa tcggaaaac agttcacga tcgagacgt 
tcacggggg caccgcgac cgcaacaac taccgacag 
cactcccac agaagcacc agaagctcc aagtctcgc 
aaaaacgac gagatggcg cccttccag acggccgtg 
accctcacc cccgacaac cgcgacggc caggaggtc 
atcgaggtg caaatcctg ccccaggac gacaactgg 
ggcgagaac accactgcc atcaccggg aacacgtcc 
gagcagtcc cagtcgacc gaagacgtc agtaactgg 
cccggtgaa cctgacacc gggctctcc ttcgtctgc 
caaagatac atggggacg gtgctaacg tacatcctt 
tcaatagtt gccttcttg agcccctta gccatggtc 
gtattgcca aaactgggg ttcttccct gcgctgtcc 
tcgcggctg ggactcgca gacagcgcg aaattgtta 
gcttgcggg gccgaatgt aaaggccag ttggtgtcg 
ttggcgttc aaaatggcc attttggcg gtcggggcc 
tgggctgtg ttcttgcgc aagccgaat gcgaccttg 
ccaaggttt ttcctgttt cgggcggct gtcctagcc 
tttgtttta gtctgtatt ttcgcctat tggctgttt 
tatatagtt caagtgacc gagactgcg cgtgccgcc 
accacaccg gaggaaacg gtggattat gaggccttg 
gtggcttac gccttaagt ctgactgat acgctactt 
tttatccat tacttggct gtagttttg atggagatc 
aggcaccaa caaccgatg tattacatc aaggttgtg 
cggagcccc gatggcgct agccgcgcc tatgccata 
ggccagtta agcatccag cgagcggct gtttgggtc 
cttgaaaag tactacact gagttcccc atttataac 
ccgtacttg gagcgcatc ccggtgtca aaatcgcgc 
aaaaactcc tcaagtttc aaattttac gaagtggat 
ggtgttaat aattcctcc ttgcaacag tcgcagtca 
cgggcgatg ttggcggcg catgcacgc cgacgtgac 
tcgagtcac aacgagcgc ttctacgaa gagcacgag 
tacgagaga cgtgtgaaa aaacgcagg gctcgactg 
atcacagcc gctgaagaa gccttcact catatcaaa 
cgcttacat aacgaccaa gtgcctgcc atcccgatg 
gacccgcac gaggccgcc caggccatt ttcccgtcg 
atggcacga gccctccag aagtattta agagtgacg 
aggcaacag ccgaggcac tccgtcgag tcgattttg 
actcattta gccaattgt ctcagtaat gatctgacc 
ccgaaagcg ttcctcgag cagtatcta gtgagcgag 
cccgttttg caaaatgat aaggaagtg aaggatgtg 
cagacgtgg gcgctgatt tgcgaggag ttgctaacg 
cgcccgatt ggagacaat acgagcttt cagttgagg 
caaaacgat gtctcgctg ctttgcacg attaaaaag 
atcccgcat tttaatatt acggaagag gttgttgat 
ccgaagagt aataagttt gttttgagg ctgaattcg 
gagacctcg gtatga
    mRNA sequence
    • >TC001197 RA
      gccatgttg cagccataa tttatgggg caattaaat 
tgctcctaa acgtaggcg tttacattt tataaactc 
cgatttagt tttcagtaa attttgaat aaatttgaa 
taaaaaagc atgtgtgga gtgtcggag ggaattggt 
tacgtgtgt ggggaaaaa acggtgcgt gttccaagt 
gccgtttga gaaaaatga tccagtttt attagaaaa 
acgcacaca agccgccgc actaattta aatgcaatt 
tgtaaacga tttacgcaa ctcttacga aacaaatcc 
caagaaaat gcattcctc ctgagcgaa acgaacccc 
ggaagtggc ctaaggctc caaataacg gaaattgtt 
tggattttt tatgcacaa atacatgtt tacgaaatg 
tcatcaacg tgaggagtc attgaactt attagtgaa 
ataatcagg atttttgac gagaattcc cgcaaaagt 
gttgataaa gaaccactc atgacagtt tcttgcaaa 
aaaaatccg gcttttggg aaaatacat gggcaacga 
aactggacg agatatgga gactgaatc cataaaatc 
ggaaaacag ttcacgatc gagacgttc acgggggca 
ccgcgaccg caacaacta ccgacagca ctcccacag 
aagcaccag aagctccaa gtctcgcaa aaacgacga 
gatggcgcc cttccagac ggccgtgac cctcacccc 
cgacaaccg cgacggcca ggaggtcat cgaggtgca 
aatcctgcc ccaggacga caactgggg cgagaacac 
cactgccat caccgggaa cacgtccga gcagtccca 
gtcgaccga agacgtcag taactggcc cggtgaacc 
tgacaccgg gctctcctt cgtctgcca aagatacat 
ggggacggt gctaacgta catcctttc aatagttgc 
cttcttgag ccccttagc catggtcgt attgccaaa 
actggggtt cttccctgc gctgtcctc gcggctggg 
actcgcaga cagcgcgaa attgttagc ttgcggggc 
cgaatgtaa aggccagtt ggtgtcgtt ggcgttcaa 
aatggccat tttggcggt cggggcctg ggctgtgtt 
cttgcgcaa gccgaatgc gaccttgcc aaggttttt 
cctgtttcg ggcggctgt cctagcctt tgttttagt 
ctgtatttt cgcctattg gctgtttta tatagttca 
agtgaccga gactgcgcg tgccgccac cacaccgga 
ggaaacggt ggattatga ggccttggt ggcttacgc 
cttaagtct gactgatac gctactttt tatccatta 
cttggctgt agttttgat ggagatcag gcaccaaca 
accgatgta ttacatcaa ggttgtgcg gagccccga 
tggcgctag ccgcgccta tgccatagg ccagttaag 
catccagcg agcggctgt ttgggtcct tgaaaagta 
ctacactga gttccccat ttataaccc gtacttgga 
gcgcatccc ggtgtcaaa atcgcgcaa aaactcctc 
aagtttcaa attttacga agtggatgg tgttaataa 
ttcctcctt gcaacagtc gcagtcacg ggcgatgtt 
ggcggcgca tgcacgccg acgtgactc gagtcacaa 
cgagcgctt ctacgaaga gcacgagta cgagagacg 
tgtgaaaaa acgcagggc tcgactgat cacagccgc 
tgaagaagc cttcactca tatcaaacg cttacataa 
cgaccaagt gcctgccat cccgatgga cccgcacga 
ggccgccca ggccatttt cccgtcgat ggcacgagc 
cctccagaa gtatttaag agtgacgag gcaacagcc 
gaggcactc cgtcgagtc gattttgac tcatttagc 
caattgtct cagtaatga tctgacccc gaaagcgtt 
cctcgagca gtatctagt gagcgagcc cgttttgca 
aaatgataa ggaagtgaa ggatgtgca gacgtgggc 
gctgatttg cgaggagtt gctaacgcg cccgattgg 
agacaatac gagctttca gttgaggca aaacgatgt 
ctcgctgct ttgcacgat taaaaagat cccgcattt 
taatattac ggaagaggt tgttgatcc gaagagtaa 
taagtttgt tttgaggct gaattcgga gacctcggt 
atgacctat agtttacga tcgacttat ttaagcaca 
tatcggttt gtagtattg tataattcg tggtcaagc 
ggttattct attcctcct taaataatt aacgttatt 
acaagactt cgtgtaact aatagggtg taaataatt 
tccgtggtt gtgtaagac caaaacgaa atttttatc 
agttttatg tgatatttt ttgcaataa tgtttattt 
cagctcaaa atactcgta atttatttt ttactcgtt 
ttatgaatg cacaaagtg taaattata gcacaagta 
attgtaagg caggtactt aagtatagc gtcaactga 
tgtagatac atttaagag gtttgagtc caaagattc 
ggcatgttt ggactgttt gtgcctaaa atttgtaag 
atatgtcag tgcaatttt ttccgtagc tctagctta 
tctcaaagg atcagtatt ctaaaaatg acattaaat 
gtagccttt tatatctct ttgtgtatg tacaccgat 
ctgtgaccg tgttttgaa taaatgtac taatttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY