Status
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Gene information
General information
TC001197 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12489647..12492431
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001197
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06817

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001197 RA
      MGNETGRDM ETESIKSEN SSRSRRSRG HRDRNNYRQ 
      HSHRSTRSS KSRKNDEMA PFQTAVTLT PDNRDGQEV 
      IEVQILPQD DNWGENTTA ITGNTSEQS QSTEDVSNW 
      PGEPDTGLS FVCQRYMGT VLTYILSIV AFLSPLAMV 
      VLPKLGFFP ALSSRLGLA DSAKLLACG AECKGQLVS 
      LAFKMAILA VGAWAVFLR KPNATLPRF FLFRAAVLA 
      FVLVCIFAY WLFYIVQVT ETARAATTP EETVDYEAL 
      VAYALSLTD TLLFIHYLA VVLMEIRHQ QPMYYIKVV 
      RSPDGASRA YAIGQLSIQ RAAVWVLEK YYTEFPIYN 
      PYLERIPVS KSRKNSSSF KFYEVDGVN NSSLQQSQS 
      RAMLAAHAR RRDSSHNER FYEEHEYER RVKKRRARL 
      ITAAEEAFT HIKRLHNDQ VPAIPMDPH EAAQAIFPS 
      MARALQKYL RVTRQQPRH SVESILTHL ANCLSNDLT 
      PKAFLEQYL VSEPVLQND KEVKDVQTW ALICEELLT 
      RPIGDNTSF QLRQNDVSL LCTIKKIPH FNITEEVVD 
      PKSNKFVLR LNSETSV
    CDS sequence
    • >TC001197 RA
      atgggcaac gaaactgga cgagatatg gagactgaa 
      tccataaaa tcggaaaac agttcacga tcgagacgt 
      tcacggggg caccgcgac cgcaacaac taccgacag 
      cactcccac agaagcacc agaagctcc aagtctcgc 
      aaaaacgac gagatggcg cccttccag acggccgtg 
      accctcacc cccgacaac cgcgacggc caggaggtc 
      atcgaggtg caaatcctg ccccaggac gacaactgg 
      ggcgagaac accactgcc atcaccggg aacacgtcc 
      gagcagtcc cagtcgacc gaagacgtc agtaactgg 
      cccggtgaa cctgacacc gggctctcc ttcgtctgc 
      caaagatac atggggacg gtgctaacg tacatcctt 
      tcaatagtt gccttcttg agcccctta gccatggtc 
      gtattgcca aaactgggg ttcttccct gcgctgtcc 
      tcgcggctg ggactcgca gacagcgcg aaattgtta 
      gcttgcggg gccgaatgt aaaggccag ttggtgtcg 
      ttggcgttc aaaatggcc attttggcg gtcggggcc 
      tgggctgtg ttcttgcgc aagccgaat gcgaccttg 
      ccaaggttt ttcctgttt cgggcggct gtcctagcc 
      tttgtttta gtctgtatt ttcgcctat tggctgttt 
      tatatagtt caagtgacc gagactgcg cgtgccgcc 
      accacaccg gaggaaacg gtggattat gaggccttg 
      gtggcttac gccttaagt ctgactgat acgctactt 
      tttatccat tacttggct gtagttttg atggagatc 
      aggcaccaa caaccgatg tattacatc aaggttgtg 
      cggagcccc gatggcgct agccgcgcc tatgccata 
      ggccagtta agcatccag cgagcggct gtttgggtc 
      cttgaaaag tactacact gagttcccc atttataac 
      ccgtacttg gagcgcatc ccggtgtca aaatcgcgc 
      aaaaactcc tcaagtttc aaattttac gaagtggat 
      ggtgttaat aattcctcc ttgcaacag tcgcagtca 
      cgggcgatg ttggcggcg catgcacgc cgacgtgac 
      tcgagtcac aacgagcgc ttctacgaa gagcacgag 
      tacgagaga cgtgtgaaa aaacgcagg gctcgactg 
      atcacagcc gctgaagaa gccttcact catatcaaa 
      cgcttacat aacgaccaa gtgcctgcc atcccgatg 
      gacccgcac gaggccgcc caggccatt ttcccgtcg 
      atggcacga gccctccag aagtattta agagtgacg 
      aggcaacag ccgaggcac tccgtcgag tcgattttg 
      actcattta gccaattgt ctcagtaat gatctgacc 
      ccgaaagcg ttcctcgag cagtatcta gtgagcgag 
      cccgttttg caaaatgat aaggaagtg aaggatgtg 
      cagacgtgg gcgctgatt tgcgaggag ttgctaacg 
      cgcccgatt ggagacaat acgagcttt cagttgagg 
      caaaacgat gtctcgctg ctttgcacg attaaaaag 
      atcccgcat tttaatatt acggaagag gttgttgat 
      ccgaagagt aataagttt gttttgagg ctgaattcg 
      gagacctcg gtatga
    mRNA sequence
    • >TC001197 RA
      gccatgttg cagccataa tttatgggg caattaaat 
      tgctcctaa acgtaggcg tttacattt tataaactc 
      cgatttagt tttcagtaa attttgaat aaatttgaa 
      taaaaaagc atgtgtgga gtgtcggag ggaattggt 
      tacgtgtgt ggggaaaaa acggtgcgt gttccaagt 
      gccgtttga gaaaaatga tccagtttt attagaaaa 
      acgcacaca agccgccgc actaattta aatgcaatt 
      tgtaaacga tttacgcaa ctcttacga aacaaatcc 
      caagaaaat gcattcctc ctgagcgaa acgaacccc 
      ggaagtggc ctaaggctc caaataacg gaaattgtt 
      tggattttt tatgcacaa atacatgtt tacgaaatg 
      tcatcaacg tgaggagtc attgaactt attagtgaa 
      ataatcagg atttttgac gagaattcc cgcaaaagt 
      gttgataaa gaaccactc atgacagtt tcttgcaaa 
      aaaaatccg gcttttggg aaaatacat gggcaacga 
      aactggacg agatatgga gactgaatc cataaaatc 
      ggaaaacag ttcacgatc gagacgttc acgggggca 
      ccgcgaccg caacaacta ccgacagca ctcccacag 
      aagcaccag aagctccaa gtctcgcaa aaacgacga 
      gatggcgcc cttccagac ggccgtgac cctcacccc 
      cgacaaccg cgacggcca ggaggtcat cgaggtgca 
      aatcctgcc ccaggacga caactgggg cgagaacac 
      cactgccat caccgggaa cacgtccga gcagtccca 
      gtcgaccga agacgtcag taactggcc cggtgaacc 
      tgacaccgg gctctcctt cgtctgcca aagatacat 
      ggggacggt gctaacgta catcctttc aatagttgc 
      cttcttgag ccccttagc catggtcgt attgccaaa 
      actggggtt cttccctgc gctgtcctc gcggctggg 
      actcgcaga cagcgcgaa attgttagc ttgcggggc 
      cgaatgtaa aggccagtt ggtgtcgtt ggcgttcaa 
      aatggccat tttggcggt cggggcctg ggctgtgtt 
      cttgcgcaa gccgaatgc gaccttgcc aaggttttt 
      cctgtttcg ggcggctgt cctagcctt tgttttagt 
      ctgtatttt cgcctattg gctgtttta tatagttca 
      agtgaccga gactgcgcg tgccgccac cacaccgga 
      ggaaacggt ggattatga ggccttggt ggcttacgc 
      cttaagtct gactgatac gctactttt tatccatta 
      cttggctgt agttttgat ggagatcag gcaccaaca 
      accgatgta ttacatcaa ggttgtgcg gagccccga 
      tggcgctag ccgcgccta tgccatagg ccagttaag 
      catccagcg agcggctgt ttgggtcct tgaaaagta 
      ctacactga gttccccat ttataaccc gtacttgga 
      gcgcatccc ggtgtcaaa atcgcgcaa aaactcctc 
      aagtttcaa attttacga agtggatgg tgttaataa 
      ttcctcctt gcaacagtc gcagtcacg ggcgatgtt 
      ggcggcgca tgcacgccg acgtgactc gagtcacaa 
      cgagcgctt ctacgaaga gcacgagta cgagagacg 
      tgtgaaaaa acgcagggc tcgactgat cacagccgc 
      tgaagaagc cttcactca tatcaaacg cttacataa 
      cgaccaagt gcctgccat cccgatgga cccgcacga 
      ggccgccca ggccatttt cccgtcgat ggcacgagc 
      cctccagaa gtatttaag agtgacgag gcaacagcc 
      gaggcactc cgtcgagtc gattttgac tcatttagc 
      caattgtct cagtaatga tctgacccc gaaagcgtt 
      cctcgagca gtatctagt gagcgagcc cgttttgca 
      aaatgataa ggaagtgaa ggatgtgca gacgtgggc 
      gctgatttg cgaggagtt gctaacgcg cccgattgg 
      agacaatac gagctttca gttgaggca aaacgatgt 
      ctcgctgct ttgcacgat taaaaagat cccgcattt 
      taatattac ggaagaggt tgttgatcc gaagagtaa 
      taagtttgt tttgaggct gaattcgga gacctcggt 
      atgacctat agtttacga tcgacttat ttaagcaca 
      tatcggttt gtagtattg tataattcg tggtcaagc 
      ggttattct attcctcct taaataatt aacgttatt 
      acaagactt cgtgtaact aatagggtg taaataatt 
      tccgtggtt gtgtaagac caaaacgaa atttttatc 
      agttttatg tgatatttt ttgcaataa tgtttattt 
      cagctcaaa atactcgta atttatttt ttactcgtt 
      ttatgaatg cacaaagtg taaattata gcacaagta 
      attgtaagg caggtactt aagtatagc gtcaactga 
      tgtagatac atttaagag gtttgagtc caaagattc 
      ggcatgttt ggactgttt gtgcctaaa atttgtaag 
      atatgtcag tgcaatttt ttccgtagc tctagctta 
      tctcaaagg atcagtatt ctaaaaatg acattaaat 
      gtagccttt tatatctct ttgtgtatg tacaccgat 
      ctgtgaccg tgttttgaa taaatgtac taatttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY