| General information | |||
| TC001197 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12489647..12492431 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001197 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06817 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001197 RA
MGNETGRDM ETESIKSEN SSRSRRSRG HRDRNNYRQ HSHRSTRSS KSRKNDEMA PFQTAVTLT PDNRDGQEV IEVQILPQD DNWGENTTA ITGNTSEQS QSTEDVSNW PGEPDTGLS FVCQRYMGT VLTYILSIV AFLSPLAMV VLPKLGFFP ALSSRLGLA DSAKLLACG AECKGQLVS LAFKMAILA VGAWAVFLR KPNATLPRF FLFRAAVLA FVLVCIFAY WLFYIVQVT ETARAATTP EETVDYEAL VAYALSLTD TLLFIHYLA VVLMEIRHQ QPMYYIKVV RSPDGASRA YAIGQLSIQ RAAVWVLEK YYTEFPIYN PYLERIPVS KSRKNSSSF KFYEVDGVN NSSLQQSQS RAMLAAHAR RRDSSHNER FYEEHEYER RVKKRRARL ITAAEEAFT HIKRLHNDQ VPAIPMDPH EAAQAIFPS MARALQKYL RVTRQQPRH SVESILTHL ANCLSNDLT PKAFLEQYL VSEPVLQND KEVKDVQTW ALICEELLT RPIGDNTSF QLRQNDVSL LCTIKKIPH FNITEEVVD PKSNKFVLR LNSETSV
- >TC001197 RA
atgggcaac gaaactgga cgagatatg gagactgaa tccataaaa tcggaaaac agttcacga tcgagacgt tcacggggg caccgcgac cgcaacaac taccgacag cactcccac agaagcacc agaagctcc aagtctcgc aaaaacgac gagatggcg cccttccag acggccgtg accctcacc cccgacaac cgcgacggc caggaggtc atcgaggtg caaatcctg ccccaggac gacaactgg ggcgagaac accactgcc atcaccggg aacacgtcc gagcagtcc cagtcgacc gaagacgtc agtaactgg cccggtgaa cctgacacc gggctctcc ttcgtctgc caaagatac atggggacg gtgctaacg tacatcctt tcaatagtt gccttcttg agcccctta gccatggtc gtattgcca aaactgggg ttcttccct gcgctgtcc tcgcggctg ggactcgca gacagcgcg aaattgtta gcttgcggg gccgaatgt aaaggccag ttggtgtcg ttggcgttc aaaatggcc attttggcg gtcggggcc tgggctgtg ttcttgcgc aagccgaat gcgaccttg ccaaggttt ttcctgttt cgggcggct gtcctagcc tttgtttta gtctgtatt ttcgcctat tggctgttt tatatagtt caagtgacc gagactgcg cgtgccgcc accacaccg gaggaaacg gtggattat gaggccttg gtggcttac gccttaagt ctgactgat acgctactt tttatccat tacttggct gtagttttg atggagatc aggcaccaa caaccgatg tattacatc aaggttgtg cggagcccc gatggcgct agccgcgcc tatgccata ggccagtta agcatccag cgagcggct gtttgggtc cttgaaaag tactacact gagttcccc atttataac ccgtacttg gagcgcatc ccggtgtca aaatcgcgc aaaaactcc tcaagtttc aaattttac gaagtggat ggtgttaat aattcctcc ttgcaacag tcgcagtca cgggcgatg ttggcggcg catgcacgc cgacgtgac tcgagtcac aacgagcgc ttctacgaa gagcacgag tacgagaga cgtgtgaaa aaacgcagg gctcgactg atcacagcc gctgaagaa gccttcact catatcaaa cgcttacat aacgaccaa gtgcctgcc atcccgatg gacccgcac gaggccgcc caggccatt ttcccgtcg atggcacga gccctccag aagtattta agagtgacg aggcaacag ccgaggcac tccgtcgag tcgattttg actcattta gccaattgt ctcagtaat gatctgacc ccgaaagcg ttcctcgag cagtatcta gtgagcgag cccgttttg caaaatgat aaggaagtg aaggatgtg cagacgtgg gcgctgatt tgcgaggag ttgctaacg cgcccgatt ggagacaat acgagcttt cagttgagg caaaacgat gtctcgctg ctttgcacg attaaaaag atcccgcat tttaatatt acggaagag gttgttgat ccgaagagt aataagttt gttttgagg ctgaattcg gagacctcg gtatga
- >TC001197 RA
gccatgttg cagccataa tttatgggg caattaaat tgctcctaa acgtaggcg tttacattt tataaactc cgatttagt tttcagtaa attttgaat aaatttgaa taaaaaagc atgtgtgga gtgtcggag ggaattggt tacgtgtgt ggggaaaaa acggtgcgt gttccaagt gccgtttga gaaaaatga tccagtttt attagaaaa acgcacaca agccgccgc actaattta aatgcaatt tgtaaacga tttacgcaa ctcttacga aacaaatcc caagaaaat gcattcctc ctgagcgaa acgaacccc ggaagtggc ctaaggctc caaataacg gaaattgtt tggattttt tatgcacaa atacatgtt tacgaaatg tcatcaacg tgaggagtc attgaactt attagtgaa ataatcagg atttttgac gagaattcc cgcaaaagt gttgataaa gaaccactc atgacagtt tcttgcaaa aaaaatccg gcttttggg aaaatacat gggcaacga aactggacg agatatgga gactgaatc cataaaatc ggaaaacag ttcacgatc gagacgttc acgggggca ccgcgaccg caacaacta ccgacagca ctcccacag aagcaccag aagctccaa gtctcgcaa aaacgacga gatggcgcc cttccagac ggccgtgac cctcacccc cgacaaccg cgacggcca ggaggtcat cgaggtgca aatcctgcc ccaggacga caactgggg cgagaacac cactgccat caccgggaa cacgtccga gcagtccca gtcgaccga agacgtcag taactggcc cggtgaacc tgacaccgg gctctcctt cgtctgcca aagatacat ggggacggt gctaacgta catcctttc aatagttgc cttcttgag ccccttagc catggtcgt attgccaaa actggggtt cttccctgc gctgtcctc gcggctggg actcgcaga cagcgcgaa attgttagc ttgcggggc cgaatgtaa aggccagtt ggtgtcgtt ggcgttcaa aatggccat tttggcggt cggggcctg ggctgtgtt cttgcgcaa gccgaatgc gaccttgcc aaggttttt cctgtttcg ggcggctgt cctagcctt tgttttagt ctgtatttt cgcctattg gctgtttta tatagttca agtgaccga gactgcgcg tgccgccac cacaccgga ggaaacggt ggattatga ggccttggt ggcttacgc cttaagtct gactgatac gctactttt tatccatta cttggctgt agttttgat ggagatcag gcaccaaca accgatgta ttacatcaa ggttgtgcg gagccccga tggcgctag ccgcgccta tgccatagg ccagttaag catccagcg agcggctgt ttgggtcct tgaaaagta ctacactga gttccccat ttataaccc gtacttgga gcgcatccc ggtgtcaaa atcgcgcaa aaactcctc aagtttcaa attttacga agtggatgg tgttaataa ttcctcctt gcaacagtc gcagtcacg ggcgatgtt ggcggcgca tgcacgccg acgtgactc gagtcacaa cgagcgctt ctacgaaga gcacgagta cgagagacg tgtgaaaaa acgcagggc tcgactgat cacagccgc tgaagaagc cttcactca tatcaaacg cttacataa cgaccaagt gcctgccat cccgatgga cccgcacga ggccgccca ggccatttt cccgtcgat ggcacgagc cctccagaa gtatttaag agtgacgag gcaacagcc gaggcactc cgtcgagtc gattttgac tcatttagc caattgtct cagtaatga tctgacccc gaaagcgtt cctcgagca gtatctagt gagcgagcc cgttttgca aaatgataa ggaagtgaa ggatgtgca gacgtgggc gctgatttg cgaggagtt gctaacgcg cccgattgg agacaatac gagctttca gttgaggca aaacgatgt ctcgctgct ttgcacgat taaaaagat cccgcattt taatattac ggaagaggt tgttgatcc gaagagtaa taagtttgt tttgaggct gaattcgga gacctcggt atgacctat agtttacga tcgacttat ttaagcaca tatcggttt gtagtattg tataattcg tggtcaagc ggttattct attcctcct taaataatt aacgttatt acaagactt cgtgtaact aatagggtg taaataatt tccgtggtt gtgtaagac caaaacgaa atttttatc agttttatg tgatatttt ttgcaataa tgtttattt cagctcaaa atactcgta atttatttt ttactcgtt ttatgaatg cacaaagtg taaattata gcacaagta attgtaagg caggtactt aagtatagc gtcaactga tgtagatac atttaagag gtttgagtc caaagattc ggcatgttt ggactgttt gtgcctaaa atttgtaag atatgtcag tgcaatttt ttccgtagc tctagctta tctcaaagg atcagtatt ctaaaaatg acattaaat gtagccttt tatatctct ttgtgtatg tacaccgat ctgtgaccg tgttttgaa taaatgtac taatttgta
