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Gene information
General information
TC000393 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12164000..12168837
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000393
    iBeetle screen iB_06806

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    prothoracic odoriferous glands colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000393 RA
      MGLDFIEGQ GEDFERALE STGFGKFHF ALLTICGLI 
      YLNTAVGIT IISFVLPSA TCDFEMSSS DKGWLTAAP 
      MLGMVIGSY FWGCLADTK GRKTVLIGA LLMDGCCGL 
      LSSISQVYW LFMLFRFLN GFAITGAMG ICFPYLGEF 
      QPGKYRETI LCWMELFWT LGVIVLPGI AWIIIPMEF 
      VYQTDNFKF ASWNLFVAT CSIPSLFIG LWLFFFPES 
      PKFLLECGE AEEALEVLK DMYASNTGD SAANFPVIS 
      LREKVRTMS VVSQQSTRS IRSLRIRKP KELKLLLRE 
      IWEQTKALC KPPHLRYTI ITCLIQFGL TTSYYTLMI 
      WFPELFYRF EEFENLHPN EKATVCEVS SVVVPVNMT 
      TPSHEEFCG EPIADSVFL HTLIIGLAC IPTSFWLPL 
      CVHRLGAKF FLVFSLLVA GAVTFGLYF VNSATSNLV 
      LSCIFEALT SLGISTVYC VMVDLFPTN LRVMAAALS 
      LTFGRGGAL IGNLLFGFL IDLNCVVPI VLFAAMLLG 
      SGLLCLMLP NTGQGALD
    CDS sequence
    • >TC000393 RA
      atgggatta gacttcatc gagggccaa ggcgaagac 
      ttcgaaagg gcgctcgag agcacaggt ttcggaaaa 
      tttcatttc gccttgttg acaatatgt ggtttaatt 
      tatttgaac accgccgtt ggaataaca ataatctcg 
      ttcgttctg ccatcggcg acatgcgac ttcgagatg 
      tcctcttcg gacaaagga tggctgaca gccgccccc 
      atgcttggt atggtgatt ggctcgtat ttctgggga 
      tgcctggct gacaccaaa ggacgcaaa acagtatta 
      atcggtgcc ttgctcatg gacggctgc tgcggcctc 
      ttgtcgagc atttcccaa gtctattgg ctgttcatg 
      ctgttcaga ttcctaaac ggatttgcc ataacaggt 
      gcaatgggc atctgtttt ccctacttg ggcgaattc 
      cagcccggc aagtaccgc gagacgatc ttatgttgg 
      atggaactc ttctggact ctcggcgtc attgtcctg 
      ccgggaatc gcctggatc ataattcct atggaattt 
      gtataccaa actgataat tttaagttt gctagttgg 
      aacttgttc gttgccacg tgctcgatc ccgagcctt 
      ttcatcgga ctttggctg tttttcttc ccggaaagt 
      cccaagttt ctgctggag tgtggcgaa gccgaggag 
      gctctcgaa gtcctcaag gatatgtac gcaagtaac 
      acgggagac agcgctgcc aattttccg gtgattagt 
      ttaagagaa aaagttcgc acaatgagt gtggtgtct 
      caacaatcc acgcgcagt attcgtagc ttaaggatc 
      cgaaagccg aaagaactt aaactgctt ttgagagaa 
      atctgggag cagaccaag gccttgtgc aagccgccg 
      catctcaga tataccata attacgtgt ctgatccaa 
      ttcggccta actacaagt tactacact ttgatgatt 
      tggttcccg gagcttttc taccggttt gaagagttc 
      gaaaatctt catccgaac gaaaaagcc acagtgtgt 
      gaagtctcc tcggtcgtg gttcctgtc aatatgacc 
      acaccatcg cacgaggaa ttctgtggg gaaccaatc 
      gccgattcc gtttttctt cacactcta attatcgga 
      cttgcttgt attccgaca agcttttgg ttgccgttg 
      tgcgtgcac aggctgggg gctaaattt ttcttagtt 
      ttcagtcta cttgttgct ggagctgtg acttttggt 
      ttgtacttc gtcaactca gcaacgtca aacctggtc 
      ctttcgtgt atttttgaa gctctgacc agtttggga 
      atcagcacc gtttattgt gtcatggtg gacttgttc 
      ccaaccaac ttgagggta atggctgct gctttgtcg 
      ctaacgttt ggtcgagga ggtgctctt attggtaat 
      ctcttgttc ggctttctc attgatttg aactgcgtc 
      gtccctatt gtattattc gcagctatg ttattagga 
      agcggcttg ttgtgtcta atgttgcca aacacgggc 
      cagggagcg ttggactag
    mRNA sequence
    • >TC000393 RA
      gctgaattt aagacccag atgtgcgct ttaacgtct 
      agtaaatta tccacgaga cgatttaac aattcgaat 
      aaaagtagc gagaaagaa attagaacg caataattt 
      tctccgtga gcataaagt attttaatg gaacattaa 
      tgtggtcca aattactac tccataaca caggtgaca 
      tgtgtaaac ttaattaat gggattaat ttaaccatc 
      gattatttc tatttttag ttgaacttg tcactagac 
      ccatcattt ttattatac gactgccac aagcacgga 
      tgtcacgag aaagaaaac ttaatccgt cttaagccg 
      ccttaaagt ctctaaatt aataagcgc gagtgcgag 
      tcgagtgat gggtaaaaa ccccaaccg atgcaaaaa 
      tcgaaaata accaatctg ggagtagat gaacttgag 
      ataatgact gataatgga catttgacc aatgaaaat 
      cggtattat gcgaaggta acgtactgc gggatatga 
      tatttacaa tcgcggcta aataatatt attgtactg 
      tgagtttct tcgagtgac acctgtaat tattcggat 
      tttgtattg gagcgtttg ctgcgacac caacatggg 
      attagactt catcgaggg ccaaggcga agacttcga 
      aagggcgct cgagagcac aggtttcgg aaaatttca 
      tttcgcctt gttgacaat atgtggttt aatttattt 
      gaacaccgc cgttggaat aacaataat ctcgttcgt 
      tctgccatc ggcgacatg cgacttcga gatgtcctc 
      ttcggacaa aggatggct gacagccgc ccccatgct 
      tggtatggt gattggctc gtatttctg gggatgcct 
      ggctgacac caaaggacg caaaacagt attaatcgg 
      tgccttgct catggacgg ctgctgcgg cctcttgtc 
      gagcatttc ccaagtcta ttggctgtt catgctgtt 
      cagattcct aaacggatt tgccataac aggtgcaat 
      gggcatctg ttttcccta cttgggcga attccagcc 
      cggcaagta ccgcgagac gatcttatg ttggatgga 
      actcttctg gactctcgg cgtcattgt cctgccggg 
      aatcgcctg gatcataat tcctatgga atttgtata 
      ccaaactga taattttaa gtttgctag ttggaactt 
      gttcgttgc cacgtgctc gatcccgag ccttttcat 
      cggactttg gctgttttt cttcccgga aagtcccaa 
      gtttctgct ggagtgtgg cgaagccga ggaggctct 
      cgaagtcct caaggatat gtacgcaag taacacggg 
      agacagcgc tgccaattt tccggtgat tagtttaag 
      agaaaaagt tcgcacaat gagtgtggt gtctcaaca 
      atccacgcg cagtattcg tagcttaag gatccgaaa 
      gccgaaaga acttaaact gcttttgag agaaatctg 
      ggagcagac caaggcctt gtgcaagcc gccgcatct 
      cagatatac cataattac gtgtctgat ccaattcgg 
      cctaactac aagttacta cactttgat gatttggtt 
      cccggagct tttctaccg gtttgaaga gttcgaaaa 
      tcttcatcc gaacgaaaa agccacagt gtgtgaagt 
      ctcctcggt cgtggttcc tgtcaatat gaccacacc 
      atcgcacga ggaattctg tggggaacc aatcgccga 
      ttccgtttt tcttcacac tctaattat cggacttgc 
      ttgtattcc gacaagctt ttggttgcc gttgtgcgt 
      gcacaggct gggggctaa atttttctt agttttcag 
      tctacttgt tgctggagc tgtgacttt tggtttgta 
      cttcgtcaa ctcagcaac gtcaaacct ggtcctttc 
      gtgtatttt tgaagctct gaccagttt gggaatcag 
      caccgttta ttgtgtcat ggtggactt gttcccaac 
      caacttgag ggtaatggc tgctgcttt gtcgctaac 
      gtttggtcg aggaggtgc tcttattgg taatctctt 
      gttcggctt tctcattga tttgaactg cgtcgtccc 
      tattgtatt attcgcagc tatgttatt aggaagcgg 
      cttgttgtg tctaatgtt gccaaacac gggccaggg 
      agcgttgga ctaggatca ctttagttt ttaatattt 
      tttcaatta tataacttt gtgataatt gtactttat 
      tgacaacaa aatacctga atcgcattc gatttttta 
      ggtttaaat gtacgtaca ataatgtaa aatgataat 
      tatttttgt taggagaat gttttattt atagttaac 
      ttatttact tgtaaactt gtaagatat ttatttata 
      tttatttta tatagaaat acagcactt ttctgagcg 
      agattgttg tttctgagg gaggttgta gggacaagt 
      gccttagat aggactcta tttttttaa taattattg 
      ttttaccag catttaggc ccaaacctt cgaaagatt 
      tcacaaagt atttcacgc ctaagtttg tgttatcaa 
      gtgtacatt cttatttta gtttatctt tatcaactg 
      tattacccc aaaacacga ataaaaatt ttacccata 
      a
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