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Gene information
General information
TC011315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3200294..3202666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011315
    iBeetle screen iB_06798

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: larvae are not able to shed off old skin
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011315 RA
      MTQPSTGNT CLPESEMPE AESSGVLES VKQKKYSSG 
      SLGGDITRP KVVTVKHPE SNKPKPTAK KNKPIQADL 
      DVSKEFLRC RDEGLKRLD LSKSNITHL PPTVRDLIH 
      LSELYLYGN KLVYLPLEI GCLTNLQTL ALSENSLTS 
      LPDSLMSLK SLKVLDLRH NKLNDIPDV VYKLTSLTT 
      LFLRFNRIR YVDDEIRFL TSLTMLSLR ENKIKELPA 
      GIGKLVNLV TFDVSHNHL EHLPEEIGQ CINLSTLDL 
      QHNELLDIP DTIGELQQL TRLGLRYNR LSFIPSSLS 
      NCRHMDEFN VEGNAISQL PEGLLSSLS ELTSITLSR 
      NNFAAYPSG GPSQFTNVD SINLEHNQI DKIPYGIFS 
      RAKHLTKLN MKENQLTSL PLDVGTWTN MVELNLGTN 
      QLGKLPDDI QALQALEVL VLSNNLLRR IPPSVGNLR 
      KLRVLDLEE NRLEQLPNE IGYLRDLQR LIVQSNQLT 
      SLPRAIGHL ANLVFLSVG ENNLAYLPE EIGTLENLE 
      SLYVNDNPS LHNLPFELA LCSNLQIMS IENCPLSQI 
      PAEIVAGGP SLVIQYLKM QGPYRAM
    CDS sequence
    • >TC011315 RA
      atgactcaa ccttcaacg gggaacacg tgtctgcca 
      gaatccgaa atgccagaa gctgaatcc tcaggtgtc 
      ttagaaagc gtcaagcag aagaagtac tcgtcgggc 
      tcgctgggg ggcgacatc acgcgcccc aaggtggtg 
      acggtgaag cacccggag tcgaacaag ccgaagccc 
      acagcgaag aagaacaag ccgatccag gccgacctg 
      gacgtctcc aaggagttt ctcaggtgc agggacgag 
      ggcctgaag cggcttgac ttgagtaag tccaacatc 
      acgcatctg cccccgact gtcagggat ctgatccac 
      ctctccgag ctgtacctg tacggcaac aagctggtc 
      tacctgccg ctcgagatc ggctgcctg accaacctg 
      cagacgctc gccttgagc gagaactcg ctgacgagc 
      ctcccagac tcgctcatg agcctcaag tcgctgaaa 
      gtgctcgac ttgaggcac aacaaactc aacgatatc 
      cccgatgtt gtgtacaaa ttaacgtca ctgacgacg 
      ctcttcttg cggtttaac cgaattcgt tacgtcgac 
      gacgaaatc aggtttctc acctcgtta acgatgctc 
      agtttacgg gaaaataaa attaaagaa ttgccggca 
      gggatcgga aaactagtt aatttggtc acatttgac 
      gtttcgcac aatcacctc gaacacctg cccgaggaa 
      atcgggcag tgtattaat ttgtccacc cttgatttg 
      caacataac gagcttttg gacatccct gacacgata 
      ggggaatta cagcaattg acgcggtta gggttacgt 
      tacaatcgt ttgtcgttt attccctcc agtttgagt 
      aactgtaga cacatggat gagtttaat gttgagggt 
      aacgcgatt tcgcagttg ccggaaggt ttgttgtcc 
      agtttaagc gaattgacg agtattacg ctctcgcgg 
      aataacttc gcagcgtac ccctcaggg gggccctcg 
      cagttcacg aatgtcgat tcgattaat ctggaacac 
      aaccagatt gataaaatt ccgtatggg attttttcg 
      cgggcgaaa cacttgact aagttgaat atgaaggag 
      aatcagttg acgtcgctg ccgctagat gtcgggacg 
      tggacgaat atggtcgag ttgaatttg gggacgaat 
      cagttgggg aaattaccg gacgatatt caggctctc 
      caagcgttg gaggtttta gtattgtcg aataatttg 
      ctgaggcgg attccgccc agtgtgggg aatttgagg 
      aagttgcgg gtgttggac ttggaggag aaccggctg 
      gagcagttg ccgaacgag atcgggtat ttgagggac 
      ttgcagagg ttgatagtg cagagtaat cagctgacg 
      tcgctgccg agggccatt ggccatctg gcgaattta 
      gtatttttg agcgtgggg gagaataat ttggcgtat 
      ttgccggag gagatcggg acgttggag aatttagaa 
      tcgttgtat gttaatgat aacccgtcg ttgcataat 
      ttaccgttt gaattggcg ctatgttca aatttacaa 
      atcatgagt atagagaat tgtccgctg tcacaaatt 
      cctgccgaa attgtggca ggggggccg tcgcttgtt 
      atccaatat ttaaaaatg caagggcct tatcgtgcc 
      atgtga
    mRNA sequence
    • >TC011315 RA
      ccccgaaca cacgtcgag tgacagttg tcggaaaat 
      ggcaacgcg attgcagaa aaactcgcc tcaaagttg 
      aattttcca ctcaattgt tggaaaaag cggctccag 
      ggtggccaa atccgtgcg aatcgcccc gtttccaag 
      tcccgctct ctcagtgtg tgttctttg ttgcattcc 
      gtgcgcttg gcttgatgt tgtgtttat aatcaagag 
      ggcggcggc accggcagc tgtcacatc agcagcccc 
      cttgcaagt cacctcata caattacaa cgatgttgc 
      aaacctctc tgctcgaaa tcttccaaa atgtttcac 
      gcaatgaac actgccgaa gcaattaac tgtcgcaca 
      aactaactg gtcttcgga atgactcaa ccttcaacg 
      gggaacacg tgtctgcca gaatccgaa atgccagaa 
      gctgaatcc tcaggtgtc ttagaaagc gtcaagcag 
      aagaagtac tcgtcgggc tcgctgggg ggcgacatc 
      acgcgcccc aaggtggtg acggtgaag cacccggag 
      tcgaacaag ccgaagccc acagcgaag aagaacaag 
      ccgatccag gccgacctg gacgtctcc aaggagttt 
      ctcaggtgc agggacgag ggcctgaag cggcttgac 
      ttgagtaag tccaacatc acgcatctg cccccgact 
      gtcagggat ctgatccac ctctccgag ctgtacctg 
      tacggcaac aagctggtc tacctgccg ctcgagatc 
      ggctgcctg accaacctg cagacgctc gccttgagc 
      gagaactcg ctgacgagc ctcccagac tcgctcatg 
      agcctcaag tcgctgaaa gtgctcgac ttgaggcac 
      aacaaactc aacgatatc cccgatgtt gtgtacaaa 
      ttaacgtca ctgacgacg ctcttcttg cggtttaac 
      cgaattcgt tacgtcgac gacgaaatc aggtttctc 
      acctcgtta acgatgctc agtttacgg gaaaataaa 
      attaaagaa ttgccggca gggatcgga aaactagtt 
      aatttggtc acatttgac gtttcgcac aatcacctc 
      gaacacctg cccgaggaa atcgggcag tgtattaat 
      ttgtccacc cttgatttg caacataac gagcttttg 
      gacatccct gacacgata ggggaatta cagcaattg 
      acgcggtta gggttacgt tacaatcgt ttgtcgttt 
      attccctcc agtttgagt aactgtaga cacatggat 
      gagtttaat gttgagggt aacgcgatt tcgcagttg 
      ccggaaggt ttgttgtcc agtttaagc gaattgacg 
      agtattacg ctctcgcgg aataacttc gcagcgtac 
      ccctcaggg gggccctcg cagttcacg aatgtcgat 
      tcgattaat ctggaacac aaccagatt gataaaatt 
      ccgtatggg attttttcg cgggcgaaa cacttgact 
      aagttgaat atgaaggag aatcagttg acgtcgctg 
      ccgctagat gtcgggacg tggacgaat atggtcgag 
      ttgaatttg gggacgaat cagttgggg aaattaccg 
      gacgatatt caggctctc caagcgttg gaggtttta 
      gtattgtcg aataatttg ctgaggcgg attccgccc 
      agtgtgggg aatttgagg aagttgcgg gtgttggac 
      ttggaggag aaccggctg gagcagttg ccgaacgag 
      atcgggtat ttgagggac ttgcagagg ttgatagtg 
      cagagtaat cagctgacg tcgctgccg agggccatt 
      ggccatctg gcgaattta gtatttttg agcgtgggg 
      gagaataat ttggcgtat ttgccggag gagatcggg 
      acgttggag aatttagaa tcgttgtat gttaatgat 
      aacccgtcg ttgcataat ttaccgttt gaattggcg 
      ctatgttca aatttacaa atcatgagt atagagaat 
      tgtccgctg tcacaaatt cctgccgaa attgtggca 
      ggggggccg tcgcttgtt atccaatat ttaaaaatg 
      caagggcct tatcgtgcc atgtgattg tcttaaaat 
      atgtcataa ccagtgtgt ctgttagtt gttattaat 
      caatgttga taaagaaaa gacattatc tttatattt 
      agaataatt gttccgagt tctttgtgt tgtagtcgt 
      cctttcgcc catcggttc atttactgg attatttaa 
      aatagatca ttaatgacc ggtttccac aacattttt 
      atcggttcc gtaacttta ccctagcag tggctttaa 
      aataataaa atgataatt tataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY