Status
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Gene information
General information
TC032172 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11092404..11096700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032172
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06792

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032172 RA
      MWKCHKCGK PVYFAERKQ SLGYNWHPE CLRCEECGK 
      RLNPGQHAE HKGVPYCHV PCYGALFGP QLFGHGTRV 
      ESHKSFGLQ KDVPKVGNG PSLPRAQLE AKLKAYNQF 
      FEGKSGEVR SREVNGRLI LEGSLRIHW GVQGVIHLK 
      ENDDQRTVV TVRKRNSYR MSASTDNDS DDDVQSLSR 
      DTSYNDIST CSDVTTSLD ISELKSDTG IESGLDSTD 
      TSTAENSPT TPPLEVLPK SVTLPSKLD VKNLEWDEL 
      DELLQVERK FDESDKLYQ TMPVPLPSQ SSTDTSSST 
      QSNDSSTKT ESCAEDSSA KTLDSETLK NPVPNGSTD 
      ELSPLKKPL SKDDTWLEN SNHLNRSMS GPDCLGRIR 
      DPHSPEFDR SSIASTDIS TMSEALGDV VIRRPAKTG 
      STAIRRRPG KRLSRSKVK RRCSINGHF YDRETSFFT 
      PPHGSQMSV WVTSLVNTQ EVINLMLDK YKVDSDGSN 
      FALFVVRDN GEQRRLKED EYPLLTRVL LGPHEDVSK 
      LFLMDSHNT PEVSSEVAQ FLNLSLAEC RAILNQYYS 
      QEEKEVARL KEKYQEMRR RMLYRMDEL RSSL
    CDS sequence
    • >TC032172 RA
      atgtggaaa tgccataag tgcggcaaa cccgtatat 
      tttgcggaa agaaagcag tctctgggt tataattgg 
      cacccggag tgcttgcgg tgcgaagaa tgcggcaag 
      aggttaaat ccgggccaa cacgctgaa cacaaaggc 
      gtcccttac tgtcatgtg ccttgttat ggggccttg 
      tttggcccg cagttgttc ggacacggg accagagta 
      gagtcccac aagagcttc ggtctacag aaagatgtt 
      cctaaagtc ggaaacggg ccttcgctg cccagggcc 
      cagctggag gcaaagcta aaggcttat aaccagttt 
      tttgaaggg aagagtgga gaggtccgc agccgggaa 
      gtgaacggg cggttgatt ttagagggg tcgcttagg 
      atacactgg ggggttcag ggggtcatc cacttgaag 
      gaaaacgac gatcagcgg acggtggtc accgtcagg 
      aagcgaaac tcgtaccga atgtccgca agtacggac 
      aatgactct gatgatgat gtacaaagc ctctcgcgt 
      gatacaagt tataatgat atttctaca tgttcagat 
      gttacgact agtttagat attagtgag ttgaagtca 
      gacactggg attgaaagc gggctggat tccactgat 
      acaagcacg gctgagaac agcccaacg acgccccct 
      ttggaggtc ttgcccaaa agtgtgact ttgccgtcg 
      aaattggac gttaaaaat ttggagtgg gacgaattg 
      gacgagttg ttgcaagta gagagaaaa tttgatgag 
      tctgacaag ttatatcaa acaatgccc gtaccactg 
      ccctcacaa tcaagcact gacaccagt tcttccaca 
      caaagcaac gacagcagt acaaaaaca gaaagttgt 
      gctgaagac agcagcgcc aaaaccctc gacagcgaa 
      acgctgaaa aaccccgtt ccaaacggc tcaactgac 
      gagctttcg cctttgaaa aagccgctc agtaaagat 
      gatacttgg cttgagaac agtaaccac ttaaacaga 
      tccatgtct ggtccggat tgtttgggt cgaatccgg 
      gacccacat tccccggag ttcgacaga tcgtcgata 
      gcaagtacc gacatatca acgatgtcc gaagcttta 
      ggtgatgtt gttattcgg aggcctgcg aaaactggc 
      tcaacggcg atcagacgc cgacctggc aaacgatta 
      tctcgcagt aaagtgaag cgaaggtgt tcgataaat 
      ggccatttt tacgaccgt gagactagt tttttcaca 
      ccaccgcat ggcagtcag atgagtgtt tgggtgacg 
      tcgctagtc aatacgcaa gaagttata aatttaatg 
      ctagataaa tataaagtt gatagtgat gggagcaat 
      tttgcgttg tttgttgtg cgagataac ggcgagcaa 
      aggcggctg aaggaggat gaatatccg ttactaaca 
      cgtgttttg ttagggccc catgaggat gtgtccaaa 
      ttattttta atggatagt cacaatact cctgaagtt 
      agtagcgaa gtagcacaa tttttgaat ctctcttta 
      gccgaatgc cgggctatt ttaaaccag tattattcc 
      caagaagag aaggaagtt gccagactt aaagaaaaa 
      tatcaagaa atgagaagg aggatgttg tatcgaatg 
      gatgagttg aggtctagt ttataa
    mRNA sequence
    • >TC032172 RA
      cctaacatg actttgggg tacgtccgt gggatcatc 
      tgatgttgc ctgttgtgc ttcaaaggc catgtagtg 
      tctcaaatg tgatacaaa tgtttaatt tcccttgtt 
      ttgagttgt cttttgtgc ttgttcgtt ttatttcaa 
      tgtaatata atgtggaaa tgccataag tgcggcaaa 
      cccgtatat tttgcggaa agaaagcag tctctgggt 
      tataattgg cacccggag tgcttgcgg tgcgaagaa 
      tgcggcaag aggttaaat ccgggccaa cacgctgaa 
      cacaaaggc gtcccttac tgtcatgtg ccttgttat 
      ggggccttg tttggcccg cagttgttc ggacacggg 
      accagagta gagtcccac aagagcttc ggtctacag 
      aaagatgtt cctaaagtc ggaaacggg ccttcgctg 
      cccagggcc cagctggag gcaaagcta aaggcttat 
      aaccagttt tttgaaggg aagagtgga gaggtccgc 
      agccgggaa gtgaacggg cggttgatt ttagagggg 
      tcgcttagg atacactgg ggggttcag ggggtcatc 
      cacttgaag gaaaacgac gatcagcgg acggtggtc 
      accgtcagg aagcgaaac tcgtaccga atgtccgca 
      agtacggac aatgactct gatgatgat gtacaaagc 
      ctctcgcgt gatacaagt tataatgat atttctaca 
      tgttcagat gttacgact agtttagat attagtgag 
      ttgaagtca gacactggg attgaaagc gggctggat 
      tccactgat acaagcacg gctgagaac agcccaacg 
      acgccccct ttggaggtc ttgcccaaa agtgtgact 
      ttgccgtcg aaattggac gttaaaaat ttggagtgg 
      gacgaattg gacgagttg ttgcaagta gagagaaaa 
      tttgatgag tctgacaag ttatatcaa acaatgccc 
      gtaccactg ccctcacaa tcaagcact gacaccagt 
      tcttccaca caaagcaac gacagcagt acaaaaaca 
      gaaagttgt gctgaagac agcagcgcc aaaaccctc 
      gacagcgaa acgctgaaa aaccccgtt ccaaacggc 
      tcaactgac gagctttcg cctttgaaa aagccgctc 
      agtaaagat gatacttgg cttgagaac agtaaccac 
      ttaaacaga tccatgtct ggtccggat tgtttgggt 
      cgaatccgg gacccacat tccccggag ttcgacaga 
      tcgtcgata gcaagtacc gacatatca acgatgtcc 
      gaagcttta ggtgatgtt gttattcgg aggcctgcg 
      aaaactggc tcaacggcg atcagacgc cgacctggc 
      aaacgatta tctcgcagt aaagtgaag cgaaggtgt 
      tcgataaat ggccatttt tacgaccgt gagactagt 
      tttttcaca ccaccgcat ggcagtcag atgagtgtt 
      tgggtgacg tcgctagtc aatacgcaa gaagttata 
      aatttaatg ctagataaa tataaagtt gatagtgat 
      gggagcaat tttgcgttg tttgttgtg cgagataac 
      ggcgagcaa aggcggctg aaggaggat gaatatccg 
      ttactaaca cgtgttttg ttagggccc catgaggat 
      gtgtccaaa ttattttta atggatagt cacaatact 
      cctgaagtt agtagcgaa gtagcacaa tttttgaat 
      ctctcttta gccgaatgc cgggctatt ttaaaccag 
      tattattcc caagaagag aaggaagtt gccagactt 
      aaagaaaaa tatcaagaa atgagaagg aggatgttg 
      tatcgaatg gatgagttg aggtctagt ttataattt 
      ccactgatt gccagctct cttcagtct acttaattt 
      ctcactatt tattaagag aagatatat gtataattg 
      tgtgtttta tatatttgt tatactttt ccattttta 
      aacgcctat tgttgcttt tagttcttt cattgtgtt 
      aattcacat taatttagt gtctgcaat ttttaataa 
      aaactaatg attcatcag gtctatatt tatgtaatg 
      aaggtaaat ctaaacata attgttata tgaagactg 
      taataaaac aataaatca aaataaata ttatcaaaa 
      caa
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