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Gene information
General information
TC001101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10920519..10923126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001101
    iBeetle screen iB_06790

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001101 RA
      MSKKPPKQS NKSKGKAKI KIFNNRKKA TTPESAAIE 
      KLQEKYEAI DVSTINSFD DLPLSPKTL KGLKECGYT 
      KPTDIQRET IKLGLTGKD ILGAAQTGS GKTLAFLIP 
      ILERLYCKQ WTRLDGLGA LVITPTREL AYQIFEELR 
      RVGEHHEFS AGLIIGGKD LKFERNRMD QCNIVIGTP 
      GRILQHMDE NPLFDCVNM EILVLDEAD RCLDMGFEQ 
      TMNAIVANL PAKRQTLLF SATQTKSVR DLARLSLKN 
      PAYVSVHEH SEYSTPKGL QQSYVVCEL KDKVSILWS 
      FIKNHLKQK SIIFLASCK EVKYVYEIF CRLRPGVSL 
      MALYGTLHQ LRRMDIYEN FCKKTSAVL FATDIAARG 
      LDFPEVHWV VQADCPEDA ATYIHRVGR TARFFRGGE 
      SLLLLLPSE LKMLDNLKE KKIPIEKID INPLKLNNP 
      VRKMEAFLA RDPDLKDTA QRAFVSYAK SVFLMKDKK 
      VFNVQALDT DSFAHSLGL AIPPRIRFL QRLNARNAK 
      IQQSKNKIY FNDEDEEKK IETAVSTDS GVSDDEKPS 
      APFHVSDDS DSDDGVLKV KRKDHDIDL PTDFELEEN 
      SKNKNKKPV TKAAVAKKL LKKKIVPNK KIVFDDSGE 
      AVTVGLKDK KSQLAKEYE NEDKGGIDI ETAKLVLKE 
      EDKFDKQLF KDKVKAKHK EEKRKLKEL KKEEEAEKD 
      EFGESESEN EPDLSWLPD PDKIYGKGE QEELTEETI 
      KPKKRKLKS GDTNKELPQ KNWH
    CDS sequence
    • >TC001101 RA
      atgtctaaa aaaccccca aaacagtcg aataaatcg 
      aaaggcaag gccaaaata aaaattttc aacaatagg 
      aagaaagca acgactccg gaatcggct gctattgag 
      aagcttcaa gaaaaatac gaagctatt gatgtttct 
      accattaat agtttcgat gacttacct ttatcccca 
      aagactcta aaagggctt aaagaatgt ggttacaca 
      aaacctacg gacattcaa agagaaacc ataaaattg 
      ggtctcacg gggaaagac attctaggg gcggctcag 
      actggttct ggcaaaacc ctcgcgttt cttatcccg 
      attctggaa cgcttgtat tgcaaacaa tggacgcga 
      ctggacggt cttggagcc ttagtcatc acgccaacg 
      agagagttg gcttatcag atttttgag gaattgcga 
      cgggttggg gaacaccac gaattttcc gcgggttta 
      attatcgga ggcaaagac ttaaaattt gagaggaat 
      cgaatggat cagtgtaat attgtgatt ggtacaccg 
      ggccgaatt ttacaacac atggatgaa aacccgctg 
      tttgattgc gtaaatatg gaaatactg gtcttggat 
      gaagctgat aggtgtcta gatatgggt tttgaacaa 
      accatgaac gcaattgtc gcgaatttg ccagcaaag 
      cgtcaaact ttgctgttc tcagccacg caaactaaa 
      tcagtgaga gacttggcg cgtttgagt ttgaaaaat 
      cctgcgtat gtctccgtg cacgaacac agcgaatat 
      agcactcct aaaggactg caacagagc tatgttgtc 
      tgtgagctc aaagacaaa gtttcgatt ttgtggagt 
      tttatcaaa aatcacttg aagcagaag agtatcatt 
      tttcttgcg agctgtaaa gaagttaaa tatgtctac 
      gaaatattt tgcaggcta aggcctggt gtgagctta 
      atggcattg tatggcact cttcatcaa ttaagaaga 
      atggacatt tacgaaaat ttttgtaag aaaacctct 
      gcggtgctt ttcgcaact gacatagct gccagaggt 
      ttggatttc ccagaggtt cattgggtg gttcaagcc 
      gattgtcct gaagacgca gctacgtac attcatcgc 
      gttggcaga actgctcgg tttttccga ggaggagaa 
      agtctactt cttctttta ccaagtgaa ttaaaaatg 
      ttggacaat ttgaaagaa aagaaaatt ccgattgag 
      aaaatagac ataaacccc ctgaaactc aacaatcct 
      gtgcgaaaa atggaagct tttcttgca cgtgacccc 
      gatttgaag gacacagct cagagagcg tttgtttct 
      tatgccaag tcagtgttt cttatgaag gataagaaa 
      gtattcaat gttcaggcg ttggatacc gattcgttt 
      gcacattct cttggtctg gcaatccct ccaagaatt 
      cgatttttg caaagactc aatgctagg aacgcgaaa 
      atacagcaa agtaaaaat aaaatttat ttcaacgat 
      gaggatgaa gagaaaaaa attgaaacg gccgtttcg 
      accgacagt ggtgtaagt gacgatgaa aaaccttcc 
      gctccgttc cacgtgtcc gacgatagt gatagtgat 
      gacggagtt ttaaaagtg aagagaaaa gatcacgac 
      atagactta ccaaccgat tttgaattg gaagaaaat 
      agtaaaaac aaaaacaag aaacctgtg actaaagca 
      gcagtcgct aagaaatta ctaaagaag aaaattgtt 
      ccaaacaag aaaattgtt tttgatgac agtggcgaa 
      gctgtaact gttggcttg aaagacaag aagtcacaa 
      ctggccaaa gagtacgaa aatgaagat aagggcggc 
      attgatatt gagactgcg aaattggtg ctcaaggaa 
      gaagataaa tttgacaaa cagctgttt aaagacaag 
      gtcaaggca aaacataaa gaggaaaaa cgcaaactt 
      aaggaattg aagaaggag gaagaagcg gaaaaggac 
      gagtttgga gaatcggag tctgagaac gaacccgac 
      ttatcatgg cttccagat cctgataaa atatatggg 
      aaaggggaa caagaggaa ctaacagaa gaaacaatt 
      aaacccaag aagaggaaa ttaaaatca ggagacaca 
      aataaggaa ttaccacag aagaattgg cattaa
    mRNA sequence
    • >TC001101 RA
      ttttacatg tgttctttt tgcattgat tagcataat 
      gtctaaaaa acccccaaa acagtcgaa taaatcgaa 
      aggcaaggc caaaataaa aattttcaa caataggaa 
      gaaagcaac gactccgga atcggctgc tattgagaa 
      gcttcaaga aaaatacga agctattga tgtttctac 
      cattaatag tttcgatga cttaccttt atccccaaa 
      gactctaaa agggcttaa agaatgtgg ttacacaaa 
      acctacgga cattcaaag agaaaccat aaaattggg 
      tctcacggg gaaagacat tctaggggc ggctcagac 
      tggttctgg caaaaccct cgcgtttct tatcccgat 
      tctggaacg cttgtattg caaacaatg gacgcgact 
      ggacggtct tggagcctt agtcatcac gccaacgag 
      agagttggc ttatcagat ttttgagga attgcgacg 
      ggttgggga acaccacga attttccgc gggtttaat 
      tatcggagg caaagactt aaaatttga gaggaatcg 
      aatggatca gtgtaatat tgtgattgg tacaccggg 
      ccgaatttt acaacacat ggatgaaaa cccgctgtt 
      tgattgcgt aaatatgga aatactggt cttggatga 
      agctgatag gtgtctaga tatgggttt tgaacaaac 
      catgaacgc aattgtcgc gaatttgcc agcaaagcg 
      tcaaacttt gctgttctc agccacgca aactaaatc 
      agtgagaga cttggcgcg tttgagttt gaaaaatcc 
      tgcgtatgt ctccgtgca cgaacacag cgaatatag 
      cactcctaa aggactgca acagagcta tgttgtctg 
      tgagctcaa agacaaagt ttcgatttt gtggagttt 
      tatcaaaaa tcacttgaa gcagaagag tatcatttt 
      tcttgcgag ctgtaaaga agttaaata tgtctacga 
      aatattttg caggctaag gcctggtgt gagcttaat 
      ggcattgta tggcactct tcatcaatt aagaagaat 
      ggacattta cgaaaattt ttgtaagaa aacctctgc 
      ggtgctttt cgcaactga catagctgc cagaggttt 
      ggatttccc agaggttca ttgggtggt tcaagccga 
      ttgtcctga agacgcagc tacgtacat tcatcgcgt 
      tggcagaac tgctcggtt tttccgagg aggagaaag 
      tctacttct tcttttacc aagtgaatt aaaaatgtt 
      ggacaattt gaaagaaaa gaaaattcc gattgagaa 
      aatagacat aaaccccct gaaactcaa caatcctgt 
      gcgaaaaat ggaagcttt tcttgcacg tgaccccga 
      tttgaagga cacagctca gagagcgtt tgtttctta 
      tgccaagtc agtgtttct tatgaagga taagaaagt 
      attcaatgt tcaggcgtt ggataccga ttcgtttgc 
      acattctct tggtctggc aatccctcc aagaattcg 
      atttttgca aagactcaa tgctaggaa cgcgaaaat 
      acagcaaag taaaaataa aatttattt caacgatga 
      ggatgaaga gaaaaaaat tgaaacggc cgtttcgac 
      cgacagtgg tgtaagtga cgatgaaaa accttccgc 
      tccgttcca cgtgtccga cgatagtga tagtgatga 
      cggagtttt aaaagtgaa gagaaaaga tcacgacat 
      agacttacc aaccgattt tgaattgga agaaaatag 
      taaaaacaa aaacaagaa acctgtgac taaagcagc 
      agtcgctaa gaaattact aaagaagaa aattgttcc 
      aaacaagaa aattgtttt tgatgacag tggcgaagc 
      tgtaactgt tggcttgaa agacaagaa gtcacaact 
      ggccaaaga gtacgaaaa tgaagataa gggcggcat 
      tgatattga gactgcgaa attggtgct caaggaaga 
      agataaatt tgacaaaca gctgtttaa agacaaggt 
      caaggcaaa acataaaga ggaaaaacg caaacttaa 
      ggaattgaa gaaggagga agaagcgga aaaggacga 
      gtttggaga atcggagtc tgagaacga acccgactt 
      atcatggct tccagatcc tgataaaat atatgggaa 
      aggggaaca agaggaact aacagaaga aacaattaa 
      acccaagaa gaggaaatt aaaatcagg agacacaaa 
      taaggaatt accacagaa gaattggca ttaatgtta 
      ctcagaaat aaataaacg attaatttt tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY