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Gene information
General information
TC001098 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10913529..10914477
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001098
    iBeetle screen iB_06788

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001098 RA
      MEQSESENN TELGDKKSY AGIPEAEFV 
      EDVDAYMAR PENNKNVEE VLKKLDNQH 
      AKYKFMEYN LLSKKKRLQ NQVPELKRS 
      LTMIEKLGE QKNEFETQF LLSEQVFAK 
      AKVPPTETV FLWLGANVM LEYSLKDAQ 
      ELLTQNIAA ATKNLKYVE HDLDFLRDQ 
      FTTTEVNMA RVFNWDVKR RQAAKAAAK
    CDS sequence
    • >TC001098 RA
      atggaacag tcagaatcg gaaaataac acagaactg 
      ggagacaaa aagtcctac gctggaatc cccgaagcc 
      gaatttgtc gaagatgtg gatgcgtac atggcgcgg 
      cccgaaaac aacaaaaac gtggaagaa gtgttgaaa 
      aaattagat aaccaacat gccaagtat aagtttatg 
      gaatataat ttattgtcg aagaaaaaa cggctccag 
      aaccaagtc cccgagctg aagcgttcg ttaacgatg 
      attgagaaa ctaggcgaa cagaaaaac gaattcgag 
      actcagttt ttgctcagc gaacaggtt ttcgccaaa 
      gctaaagtg ccaccaact gagacggtg ttcctgtgg 
      ctgggggcc aatgtcatg cttgaatat agtttaaaa 
      gatgctcag gagctgctc acgcagaat attgctgca 
      gctactaag aatttaaaa tatgtggaa cacgatctc 
      gactttttg agggatcag tttacgaca accgaggtc 
      aatatggcg agagtgttc aactgggac gtgaagaga 
      cgccaagct gcgaaagca gcggcaaaa tga
    mRNA sequence
    • >TC001098 RA
      ccaccaaac tttcaattg tttagtcgg tggagtggg 
      taaattttg caaaaacta gaatggaac agtcagaat 
      cggaaaata acacagaac tgggagaca aaaagtcct 
      acgctggaa tccccgaag ccgaatttg tcgaagatg 
      tggatgcgt acatggcgc ggcccgaaa acaacaaaa 
      acgtggaag aagtgttga aaaaattag ataaccaac 
      atgccaagt ataagttta tggaatata atttattgt 
      cgaagaaaa aacggctcc agaaccaag tccccgagc 
      tgaagcgtt cgttaacga tgattgaga aactaggcg 
      aacagaaaa acgaattcg agactcagt ttttgctca 
      gcgaacagg ttttcgcca aagctaaag tgccaccaa 
      ctgagacgg tgttcctgt ggctggggg ccaatgtca 
      tgcttgaat atagtttaa aagatgctc aggagctgc 
      tcacgcaga atattgctg cagctacta agaatttaa 
      aatatgtgg aacacgatc tcgactttt tgagggatc 
      agtttacga caaccgagg tcaatatgg cgagagtgt 
      tcaactggg acgtgaaga gacgccaag ctgcgaaag 
      cagcggcaa aatgaggct ccaagtgac catttagca 
      ctgttttaa ttttgttac aagatctgt cactgctgc 
      tttatatcg tccagtttt gcaaaatat attttcgcg 
      ctcgcactt ttacagtgc atgtaaatc acatgttac 
      ttccagctt gagcattta ataacttag cttttattt 
      aacccgttt ctgtgaata aaactattt ctaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY