Status
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Gene information
General information
TC031603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450377.1: 575..3273
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC031603
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06784

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031603 RA
        MKVALLYYQ LRLMEPQRI FRWFKFWEQ 
ILRNSIHLK RRFNILSQM I
      CDS sequence
      • >TC031603 RA
        atgaaagtg gcgttactg tattatcag 
ttacgtttg atggagccc cagcgaatt 
ttcagatgg ttcaaattt tgggagcag 
attttgcgg aattcaatt cacttaaaa 
cgtcgtttc aacatccta tcacagatg 
atttaa
      mRNA sequence
      • >TC031603 RA
        ccggatatg cgatgcact aagtaacaa tccgacaac 
gctgcatta ccaatattg ccagtgaac atccagtga 
aggataatg gcggccgtt tgtttacaa tagtgaagt 
aggcgaatt tgagcgtaa tttgtggtg aaagtgagt 
ttatagtga ctaaaaaat atatactga ttgtaacag 
aacaatgcc acggcagtg tttcgtttg taaaatagt 
tagtaacac gagtttgaa tttatcatt ccacaggat 
accagttga cccaagtat tagaagaat ctggttctc 
gagtgttgg tttaccttt taatgctga ggaaactgc 
tcctaaaca cgcgttaat ttgcgcaaa tcattttct 
agagacaga ttttgagta tctgggcgt cactaaaaa 
gaaagtatt gaaaaaggg agcaattcc aattgatac 
cactgacaa agatttaag tagttctga ttcgacaag 
cgtagaaac gtcagtaac atctcctaa atcacataa 
aagaagtta cgatgaagc tggtctttc aactctcaa 
gtcatctag cagcagcac cctgaccgc aagtgaagc 
tgaggacga actccgtaa agaatctaa tctccttca 
acataagaa ttcaagtga atctttggg ggtacggtc 
tcttccttc ctctggaac attaacagc ttctgagac 
taaacgatt agttagtca ggaggtaca taaagactc 
tgatctttc tcaacaaaa ggattcaag tgaatcctt 
gggggtacg gtctcttcc ttcctcttc aactttaac 
agcttctga gactgaaag attacttac taagcaagt 
acgtgtcgt tacgacaag tgaaaccaa aaaaagaag 
aagatttag tgtgggatt tctgagtag ctcagattt 
ttcaacacc aagaaaagc aaaacgaca ttttcgaat 
aatgaaaag taaattgca atgtcaaaa taaaaaagt 
taaagtgtt gcaacagtc tgtgcgaag attacggtt 
acgagtaaa ttccttaaa atcgcttgt agatgtttt 
aaaggaaaa acgcttgat tactgaaaa cgctgaatc 
tactattat gtcatcgtt gccttcaac tgctgcgga 
tatttttag acgaatgtt gaaagggcc tacaaaaca 
gaaatatac accagcgtt aagatcctt tgctcttac 
gcttagctt ttattcacc gaaagcata caactttgt 
tcgacagac ttttaatcg atctttacc gcatcttaa 
cactatctc aaagtggta tcagtcagt ggatggatc 
cccagggtg gacaaaaga agcgttaaa tgctttaaa 
aataaaaac gaaagatgt cgaatgtaa gaacaataa 
aatagtctg taaccttgt gctcgacga aatgtcgat 
cagaaaaca aatagaatg gactggaaa aaaatttac 
gggatatgt tgatattgg tacaaatat tagtgctga 
tacacttcc agaagcaaa agaagttct ggtttttat 
gcttgtaaa cgtaaatgg gacatggaa aatacccat 
tggatattt tcttctaaa tggtttatg tgccagtga 
aaaagcttc gcttgtcaa gtcatgtct tcagtttgt 
acatgaaag tggcgttac tgtattatc agttacgtt 
tgatggagc cccagcgaa ttttcagat ggttcaaat 
tttgggagc agattttgc ggaattcaa ttcacttaa 
aacgtcgtt tcaacatcc tatcacaga tgatttaat 
tttcatatt tccagatgc ttgtcatat gctgaagct 
tgtaagaaa ttgtttggc aacatattc attaattac 
tgatggcga tggtggtaa aattgaatg gaaatttct 
ggaaaaact tgttgagtt acagtgtga agaaggttt 
acatgccgg gacaaaaat aagaactcg tcacattaa 
attcgcaaa agaaaaaat gaaagtgag acttgctgc 
ccaaacctt gagcaagag tgtgtctga tgctttatt 
atttttaaa agaaagcgg gaaaactga ttttatagg 
tgcagctgc aacagcgaa gtttattaa aatttttaa 
tgatttatt tgatatgtt taactcaaa aaatagact 
agctaaata tttttataa aaagccgct ttcacctgt 
taccaaaaa cgagttttt taggtattt aattgagat 
taaggaata tataaagca gttaaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY