Status
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Gene information
General information
TC031836
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005247
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451482.1: 1275..2550
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031836
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06783

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031836 RA
      MIFCDSAKY FFLSYFRAF TSLCFSADG EYILAGGQS 
      KNVCIYNVA ESLIVKKFE ITQNRSLDA VDDFINRRK 
      LNEFGNIAL VEEREELEG GNVTLKLPG VRKGDMSSR 
      VLKPEVRVF SLQFSPTGQ QWAAATTEG LLIYSLTSG 
      LVFDPWDLQ IGITPDAVK NSIRDGDYL NALTMAMKL 
      NESALIQEV IENIPVKDV ELIVSSLSE QYIQRLLVI 
      ISSALDSSR HLEFYLIWA QTLLTVHGP KIAPQKNMA 
      YLLALEKSL VRKYDQISK ICEFNKFTI QYVMKLGDE 
      IAKKAKNQD KMELDDSNS EEEVDNNEN DDLYMSD
    CDS sequence
    • >TC031836 RA
      atgattttt tgtgattca gctaaatat ttttttctc 
      tcctacttc agggcattc acaagcttg tgtttttcg 
      gccgatggt gagtacatc cttgccgga ggccagtca 
      aagaacgtc tgtatttac aacgtggct gagtccttg 
      attgtgaaa aaattcgaa atcacccaa aaccggtca 
      ttggacgcc gttgatgat tttataaat cgacgaaaa 
      ttgaacgaa ttcggaaac attgccctc gtggaagag 
      cgtgaggag ctagaaggg ggtaacgtg actttaaaa 
      ttgccaggg gtgaggaag ggagacatg tcgagtcga 
      gttttgaaa cccgaggtg cgagtgttt tcgctgcaa 
      ttttcgcca actgggcaa caatgggcg gctgctacg 
      actgaggga cttttgatt tattccctc acttcgggg 
      ctagtgttt gatccctgg gatttgcaa atcgggatt 
      acgcccgat gccgtcaaa aactccatc agagatggg 
      gattatctc aacgcactc accatggcc atgaaattg 
      aatgaaagc gcgttaatt caagaagtg atcgagaat 
      attccggtc aaagatgtc gaactgatt gtttcgagt 
      ttgagcgaa cagtacatt cagagactt ttggtgatt 
      atttccagt gcgttagat agttcaaga cacttggaa 
      ttttatttg atttgggct cagacgtta cttaccgtt 
      cacgggccc aaaattgcc ccacaaaaa aatatggcc 
      tacttgtta gctttggag aagagttta gttaggaaa 
      tacgaccaa atctcaaaa atctgcgaa tttaacaaa 
      tttacgata caatacgtt atgaaatta ggtgacgaa 
      atagcgaag aaggcaaaa aatcaagat aaaatggag 
      ctcgatgat tcaaattcg gaagaggaa gttgataac 
      aacgaaaac gatgatttg tacatgtct gattaa
    mRNA sequence
    • >TC031836 RA
      agtgatgtg ttgctgaaa cggaggaaa aatgatttt 
      ttgtgattc agctaaata tttttttct ctcctactt 
      cagggcatt cacaagctt gtgtttttc ggccgatgg 
      tgagtacat ccttgccgg aggccagtc aaagaacgt 
      ctgtattta caacgtggc tgagtcctt gattgtgaa 
      aaaattcga aatcaccca aaaccggtc attggacgc 
      cgttgatga ttttataaa tcgacgaaa attgaacga 
      attcggaaa cattgccct cgtggaaga gcgtgagga 
      gctagaagg gggtaacgt gactttaaa attgccagg 
      ggtgaggaa gggagacat gtcgagtcg agttttgaa 
      acccgaggt gcgagtgtt ttcgctgca attttcgcc 
      aactgggca acaatgggc ggctgctac gactgaggg 
      acttttgat ttattccct cacttcggg gctagtgtt 
      tgatccctg ggatttgca aatcgggat tacgcccga 
      tgccgtcaa aaactccat cagagatgg ggattatct 
      caacgcact caccatggc catgaaatt gaatgaaag 
      cgcgttaat tcaagaagt gatcgagaa tattccggt 
      caaagatgt cgaactgat tgtttcgag tttgagcga 
      acagtacat tcagagact tttggtgat tatttccag 
      tgcgttaga tagttcaag acacttgga attttattt 
      gatttgggc tcagacgtt acttaccgt tcacgggcc 
      caaaattgc cccacaaaa aaatatggc ctacttgtt 
      agctttgga gaagagttt agttaggaa atacgacca 
      aatctcaaa aatctgcga atttaacaa atttacgat 
      acaatacgt tatgaaatt aggtgacga aatagcgaa 
      gaaggcaaa aaatcaaga taaaatgga gctcgatga 
      ttcaaattc ggaagagga agttgataa caacgaaaa 
      cgatgattt gtacatgtc tgattaatt ctctcaata 
      aaagtttga tttaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY