General information | |||
TC015970 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452070.1: 3565..7625 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC015970 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02537 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 55.56% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06766 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015970 RA
MGDNPVRKV RKSNFSTDE VDVLVDSVR ANYGILYGL NAKRATNRW IRQKAWLDI LGRVNGVSV EKRTLQEVK NKWKKCQYA LKEKGQGAL GDDAGDSPW EDYVDSMPL QERLSKMAA QERIAGQVL KSQPLSPTK LKVTRQAPR ALKPPEPPQ SPVNEEMCL RWNSHHSNM QTAFPSILS KEQYVDVTL AAEGKTLKC HRLILSSCS PYFEEILSG ISPLQHPVL FMKDIPFWI LKSLCDFMY AGEVHIFQN KLEELLTVA EALKIKGLA GKSTPPDPQ SENKETKKK NSQGKHHST PIRKHPIYN QNSFESTSQ DYDDDLLDP LDLLEPLYE EAAEEKKPA VTKPKENKY QYQPVKKPF GRRLRKRKF SEPQTREPS PPPVFSFRK GTRSRPNVK IPKYYHPDY TNPPKPVIS SQTDPLLDV DEIKTEPID VEDNMIDYS DEDHYMENS LETANCDSP IIITSAKPD KFKKIKLSK PIITEVHSV KETNPPPGG LEVIKLTMP QPEEGDKEE VVPEPEPEQ EEPLQNVVI ERTEGGVEE TEKIVEHEG QVTENGADE EKQDGESEV PVQNIQIES VVSAENGAA FAEEEEEEV KQDGGGEIP VQNLQLENV TSSENGAEE KQDGDNEVP VQVIQIENV RSEQDQEFQ EEEERRTAS LNHLEKEAM SVADAITME NQTDPPDIE EKV
- >TC015970 RB
MGDNPVRKV RKSNFSTDE VDVLVDSVR ANYGILYGL NAKRATNRW IRQKAWLDI LGRVNGVSV EKRTLQEVK NKWKKCQYA LKEKGQGAL GDDAGDSPW EDYVGRPAR SPAPRSPLL HSPPAAMRR NSRHRRQLY NAYDLDLKK SVVIFAEAN GNKEAQARY GISEANIRR WRRLKDDLF GRPRDYDRG NLRVRRRVN YTKTKTEKE EDCSSVDSM PLQERLSKM AAQERIAGQ VLKSQPLSP TKLKVTRQA PRALKPPEP PQSPVNEEM CLRWNSHHS NMQTAFPSI LSKEQYVDV TLAAEGKTL KCHRLILSS CSPYFEEIL SGISPLQHP VLFMKDIPF WILKSLCDF MYAGEVHIF QNKLEELLT VAEALKIKG LAGKSTPPD PQSENKETK KKNSQGKHH STPIRKHPI YNQNSFEST SQDYDDDLL DPLDLLEPL YEEAAEEKK PAVTKPKEN KYQYQPVKK PFGRRLRKR KFSEPQTRE PSPPPVFSF RKGTRSRPN VKIPKYYHP DYTNPPKPV ISSQTDPLL DVDEIKTEP IDVEDNMID YSDEDHYME NSLETANCD SPIIITSAK PDKFKKIKL SKPIITEVH SVKETNPPP GGLEVIKLT MPQPEEGDK EEVVPEPEP EQEEPLQNV VIERTEGGV EETEKIVEH EGQVTENGA DEEKQDGES EVPVQNIQI ESVVSAENG AAFAEEEEE EVKQDGGGE IPVQNLQLE NVTSSENGA EEKQDGDNE VPVQVIQIE NVRSEQDQE FQEEEERRT ASLNHLEKE AMSVADAIT MENQTDPPD IEEKV
- >TC015970 RA
atgggtgat aatccggtg aggaaagtg cggaaaagc aacttttcc accgacgaa gtggacgtc cttgtggac tcggtgagg gccaattac gggattttg tacggactt aacgccaaa agggccacc aatcgctgg atcagacag aaggcttgg cttgatatc ttggggagg gtgaatggg gttagcgtt gaaaaacgc accctacag gaggttaaa aataagtgg aagaagtgt cagtatgcc ctaaaggag aagggacag ggggctctc ggggacgac gctggggac agcccctgg gaggattac gttgattca atgcctttg caagagaga ttgtcaaag atggcagca caggaaaga atcgccggt caagtactg aaaagtcag ccactttca ccgacgaaa ttaaaagtg acgaggcaa gcgccgagg gctttgaaa ccaccagaa ccgccacaa tcgccggtt aatgaagag atgtgtctg aggtggaac agtcatcac tcgaatatg caaacggcc tttcctagc attttatcg aaggaacaa tacgttgac gtaacacta gctgctgaa gggaaaacg ctcaagtgt cacaggttg atcctgtcg tcttgtagc ccatatttt gaggagatt ttgagtgga attagtccc ttacaacac ccagtactt ttcatgaag gacattcct ttttggatt ttgaaatcc ctgtgcgat tttatgtac gccggggaa gtacacatt tttcagaat aaactcgag gagttacta accgtagcc gaggcactt aaaatcaaa ggtttggcc ggaaaatcc acaccccct gacccccaa tcggaaaac aaagaaacg aaaaaaaag aacagtcaa ggcaaacat cactcgaca cctatccga aaacacccg atttacaat caaaacagt ttcgaatcg actagtcag gactacgac gatgaccta ctagatccc ttagattta ctagaacca ctgtatgag gaggcagcc gaggagaaa aaaccggcg gttactaaa ccgaaagag aacaaatat caataccaa ccggttaaa aaaccattt ggcaggcgt ttgagaaag agaaagttt tcggaacca caaacacga gaaccgtct ccaccaccc gttttctcg ttccgtaaa ggcacccgt tcccgtcct aacgtcaaa atccccaaa tactaccac cctgactat actaatccc ccaaaaccg gttataagt tcccaaacc gatccgcta cttgacgtt gacgaaatt aaaaccgaa ccgattgat gtcgaagac aacatgatt gactacagt gacgaagac cactatatg gaaaattca cttgaaacg gccaattgc gacagcccg attattata acgagtgct aaacctgat aaattcaaa aaaattaaa ctaagtaaa ccgatcata accgaagtg cattcggtc aaagagacg aatccgcca cctggcggt ttggaagtg attaaacta acgatgcct caaccggaa gagggggac aaagaggag gtggttcca gaaccggaa ccggaacag gaagaaccc ctccaaaat gttgtgata gaacgtacg gaaggggga gttgaagag actgaaaaa atagttgaa catgaaggt caagtgacc gaaaatggc gccgacgag gaaaagcaa gatggcgag agtgaggtt cctgtccaa aacatacaa atcgagagt gttgtaagt gccgaaaat ggcgctgct ttcgcagaa gaagaagaa gaagaagta aagcaagat ggtgggggt gaaattcct gtccaaaac ctacaactt gagaatgtt acaagtagc gaaaatggc gccgaagaa aaacaagat ggcgataat gaggttcct gtccaagtt atacaaatt gaaaacgtt cgaagtgaa caagaccaa gagtttcaa gaggaagaa gaaagaaga acagcatcg ttgaatcat ctcgaaaag gaggccatg agtgttgct gatgctatc actatggag aatcaaacc gatccacct gatatcgag gaaaaagtg taa
- >TC015970 RB
atgggtgat aatccggtg aggaaagtg cggaaaagc aacttttcc accgacgaa gtggacgtc cttgtggac tcggtgagg gccaattac gggattttg tacggactt aacgccaaa agggccacc aatcgctgg atcagacag aaggcttgg cttgatatc ttggggagg gtgaatggg gttagcgtt gaaaaacgc accctacag gaggttaaa aataagtgg aagaagtgt cagtatgcc ctaaaggag aagggacag ggggctctc ggggacgac gctggggac agcccctgg gaggattac gttggtaga cccgctcgt tcgcccgcc cctcgctca ccccttttg cacagcccc cccgccgcc atgcgtcgc aacagccgc caccgccgc caactttac aacgcctac gatcttgat ttaaaaaaa tcggttgtg attttcgcc gaagcgaac ggtaacaag gaggcacaa gcccgttac ggtatttcg gaggcaaat attcgtcgt tggcggcgt ttaaaagac gaccttttc ggtagacca cgtgattac gatcgcggt aatttacgt gtacgtaga cgagtcaat tacacaaaa accaaaact gagaaagag gaggattgt agtagtgtc gattcaatg cctttgcaa gagagattg tcaaagatg gcagcacag gaaagaatc gccggtcaa gtactgaaa agtcagcca ctttcaccg acgaaatta aaagtgacg aggcaagcg ccgagggct ttgaaacca ccagaaccg ccacaatcg ccggttaat gaagagatg tgtctgagg tggaacagt catcactcg aatatgcaa acggccttt cctagcatt ttatcgaag gaacaatac gttgacgta acactagct gctgaaggg aaaacgctc aagtgtcac aggttgatc ctgtcgtct tgtagccca tattttgag gagattttg agtggaatt agtccctta caacaccca gtacttttc atgaaggac attcctttt tggattttg aaatccctg tgcgatttt atgtacgcc ggggaagta cacattttt cagaataaa ctcgaggag ttactaacc gtagccgag gcacttaaa atcaaaggt ttggccgga aaatccaca ccccctgac ccccaatcg gaaaacaaa gaaacgaaa aaaaagaac agtcaaggc aaacatcac tcgacacct atccgaaaa cacccgatt tacaatcaa aacagtttc gaatcgact agtcaggac tacgacgat gacctacta gatccctta gatttacta gaaccactg tatgaggag gcagccgag gagaaaaaa ccggcggtt actaaaccg aaagagaac aaatatcaa taccaaccg gttaaaaaa ccatttggc aggcgtttg agaaagaga aagttttcg gaaccacaa acacgagaa ccgtctcca ccacccgtt ttctcgttc cgtaaaggc acccgttcc cgtcctaac gtcaaaatc cccaaatac taccaccct gactatact aatccccca aaaccggtt ataagttcc caaaccgat ccgctactt gacgttgac gaaattaaa accgaaccg attgatgtc gaagacaac atgattgac tacagtgac gaagaccac tatatggaa aattcactt gaaacggcc aattgcgac agcccgatt attataacg agtgctaaa cctgataaa ttcaaaaaa attaaacta agtaaaccg atcataacc gaagtgcat tcggtcaaa gagacgaat ccgccacct ggcggtttg gaagtgatt aaactaacg atgcctcaa ccggaagag ggggacaaa gaggaggtg gttccagaa ccggaaccg gaacaggaa gaacccctc caaaatgtt gtgatagaa cgtacggaa gggggagtt gaagagact gaaaaaata gttgaacat gaaggtcaa gtgaccgaa aatggcgcc gacgaggaa aagcaagat ggcgagagt gaggttcct gtccaaaac atacaaatc gagagtgtt gtaagtgcc gaaaatggc gctgctttc gcagaagaa gaagaagaa gaagtaaag caagatggt gggggtgaa attcctgtc caaaaccta caacttgag aatgttaca agtagcgaa aatggcgcc gaagaaaaa caagatggc gataatgag gttcctgtc caagttata caaattgaa aacgttcga agtgaacaa gaccaagag tttcaagag gaagaagaa agaagaaca gcatcgttg aatcatctc gaaaaggag gccatgagt gttgctgat gctatcact atggagaat caaaccgat ccacctgat atcgaggaa aaagtgtaa
- >TC015970 RA
ttttacgca aaacggtta aaccggtcg gttacgtta cgataaacc gctttttgg ggttgtttg tgagcactg ggtgaaaat tccgggtta atggtggcc ggaagcgga ccgtatgct ctgatccat cagcgtgtt atttcgtgt ggtattttc atggatttt acggccaaa aggccgctt tttgtgctg gttttgatg taaaatggg tgataatcc ggtgaggaa agtgcggaa aagcaactt ttccaccga cgaagtgga cgtccttgt ggactcggt gagggccaa ttacgggat tttgtacgg acttaacgc caaaagggc caccaatcg ctggatcag acagaaggc ttggcttga tatcttggg gagggtgaa tggggttag cgttgaaaa acgcaccct acaggaggt taaaaataa gtggaagaa gtgtcagta tgccctaaa ggagaaggg acagggggc tctcgggga cgacgctgg ggacagccc ctgggagga ttacgttga ttcaatgcc tttgcaaga gagattgtc aaagatggc agcacagga aagaatcgc cggtcaagt actgaaaag tcagccact ttcaccgac gaaattaaa agtgacgag gcaagcgcc gagggcttt gaaaccacc agaaccgcc acaatcgcc ggttaatga agagatgtg tctgaggtg gaacagtca tcactcgaa tatgcaaac ggcctttcc tagcatttt atcgaagga acaatacgt tgacgtaac actagctgc tgaagggaa aacgctcaa gtgtcacag gttgatcct gtcgtcttg tagcccata ttttgagga gattttgag tggaattag tcccttaca acacccagt acttttcat gaaggacat tcctttttg gattttgaa atccctgtg cgattttat gtacgccgg ggaagtaca catttttca gaataaact cgaggagtt actaaccgt agccgaggc acttaaaat caaaggttt ggccggaaa atccacacc ccctgaccc ccaatcgga aaacaaaga aacgaaaaa aaagaacag tcaaggcaa acatcactc gacacctat ccgaaaaca cccgattta caatcaaaa cagtttcga atcgactag tcaggacta cgacgatga cctactaga tcccttaga tttactaga accactgta tgaggaggc agccgagga gaaaaaacc ggcggttac taaaccgaa agagaacaa atatcaata ccaaccggt taaaaaacc atttggcag gcgtttgag aaagagaaa gttttcgga accacaaac acgagaacc gtctccacc acccgtttt ctcgttccg taaaggcac ccgttcccg tcctaacgt caaaatccc caaatacta ccaccctga ctatactaa tcccccaaa accggttat aagttccca aaccgatcc gctacttga cgttgacga aattaaaac cgaaccgat tgatgtcga agacaacat gattgacta cagtgacga agaccacta tatggaaaa ttcacttga aacggccaa ttgcgacag cccgattat tataacgag tgctaaacc tgataaatt caaaaaaat taaactaag taaaccgat cataaccga agtgcattc ggtcaaaga gacgaatcc gccacctgg cggtttgga agtgattaa actaacgat gcctcaacc ggaagaggg ggacaaaga ggaggtggt tccagaacc ggaaccgga acaggaaga acccctcca aaatgttgt gatagaacg tacggaagg gggagttga agagactga aaaaatagt tgaacatga aggtcaagt gaccgaaaa tggcgccga cgaggaaaa gcaagatgg cgagagtga ggttcctgt ccaaaacat acaaatcga gagtgttgt aagtgccga aaatggcgc tgctttcgc agaagaaga agaagaaga agtaaagca agatggtgg gggtgaaat tcctgtcca aaacctaca acttgagaa tgttacaag tagcgaaaa tggcgccga agaaaaaca agatggcga taatgaggt tcctgtcca agttataca aattgaaaa cgttcgaag tgaacaaga ccaagagtt tcaagagga agaagaaag aagaacagc atcgttgaa tcatctcga aaaggaggc catgagtgt tgctgatgc tatcactat ggagaatca aaccgatcc acctgatat cgaggaaaa agtgtaaaa cttgtaaca ctaattatt aagggtttt aagcgtcta caattttgt aaataagaa tttgtttta ttttagtac aggaaagtg tattgtttt attcaaata ttcgttttt tataattgt aactagagt gtaataaca tggataata atgattaat tcgtgcttt gctttcctt ttcctgttg aataatgat tggatatgt gataatgtg gattttggc acaataaaa acgatttga tatt
- >TC015970 RB
ttttacgca aaacggtta aaccggtcg gttacgtta cgataaacc gctttttgg ggttgtttg tgagcactg ggtgaaaat tccgggtta atggtggcc ggaagcgga ccgtatgct ctgatccat cagcgtgtt atttcgtgt ggtattttc atggatttt acggccaaa aggccgctt tttgtgctg gttttgatg taaaatggg tgataatcc ggtgaggaa agtgcggaa aagcaactt ttccaccga cgaagtgga cgtccttgt ggactcggt gagggccaa ttacgggat tttgtacgg acttaacgc caaaagggc caccaatcg ctggatcag acagaaggc ttggcttga tatcttggg gagggtgaa tggggttag cgttgaaaa acgcaccct acaggaggt taaaaataa gtggaagaa gtgtcagta tgccctaaa ggagaaggg acagggggc tctcgggga cgacgctgg ggacagccc ctgggagga ttacgttgg tagacccgc tcgttcgcc cgcccctcg ctcacccct tttgcacag cccccccgc cgccatgcg tcgcaacag ccgccaccg ccgccaact ttacaacgc ctacgatct tgatttaaa aaaatcggt tgtgatttt cgccgaagc gaacggtaa caaggaggc acaagcccg ttacggtat ttcggaggc aaatattcg tcgttggcg gcgtttaaa agacgacct tttcggtag accacgtga ttacgatcg cggtaattt acgtgtacg tagacgagt caattacac aaaaaccaa aactgagaa agaggagga ttgtagtag tgtcgattc aatgccttt gcaagagag attgtcaaa gatggcagc acaggaaag aatcgccgg tcaagtact gaaaagtca gccactttc accgacgaa attaaaagt gacgaggca agcgccgag ggctttgaa accaccaga accgccaca atcgccggt taatgaaga gatgtgtct gaggtggaa cagtcatca ctcgaatat gcaaacggc ctttcctag cattttatc gaaggaaca atacgttga cgtaacact agctgctga agggaaaac gctcaagtg tcacaggtt gatcctgtc gtcttgtag cccatattt tgaggagat tttgagtgg aattagtcc cttacaaca cccagtact tttcatgaa ggacattcc tttttggat tttgaaatc cctgtgcga ttttatgta cgccgggga agtacacat ttttcagaa taaactcga ggagttact aaccgtagc cgaggcact taaaatcaa aggtttggc cggaaaatc cacaccccc tgaccccca atcggaaaa caaagaaac gaaaaaaaa gaacagtca aggcaaaca tcactcgac acctatccg aaaacaccc gatttacaa tcaaaacag tttcgaatc gactagtca ggactacga cgatgacct actagatcc cttagattt actagaacc actgtatga ggaggcagc cgaggagaa aaaaccggc ggttactaa accgaaaga gaacaaata tcaatacca accggttaa aaaaccatt tggcaggcg tttgagaaa gagaaagtt ttcggaacc acaaacacg agaaccgtc tccaccacc cgttttctc gttccgtaa aggcacccg ttcccgtcc taacgtcaa aatccccaa atactacca ccctgacta tactaatcc cccaaaacc ggttataag ttcccaaac cgatccgct acttgacgt tgacgaaat taaaaccga accgattga tgtcgaaga caacatgat tgactacag tgacgaaga ccactatat ggaaaattc acttgaaac ggccaattg cgacagccc gattattat aacgagtgc taaacctga taaattcaa aaaaattaa actaagtaa accgatcat aaccgaagt gcattcggt caaagagac gaatccgcc acctggcgg tttggaagt gattaaact aacgatgcc tcaaccgga agaggggga caaagagga ggtggttcc agaaccgga accggaaca ggaagaacc cctccaaaa tgttgtgat agaacgtac ggaaggggg agttgaaga gactgaaaa aatagttga acatgaagg tcaagtgac cgaaaatgg cgccgacga ggaaaagca agatggcga gagtgaggt tcctgtcca aaacataca aatcgagag tgttgtaag tgccgaaaa tggcgctgc tttcgcaga agaagaaga agaagaagt aaagcaaga tggtggggg tgaaattcc tgtccaaaa cctacaact tgagaatgt tacaagtag cgaaaatgg cgccgaaga aaaacaaga tggcgataa tgaggttcc tgtccaagt tatacaaat tgaaaacgt tcgaagtga acaagacca agagtttca agaggaaga agaaagaag aacagcatc gttgaatca tctcgaaaa ggaggccat gagtgttgc tgatgctat cactatgga gaatcaaac cgatccacc tgatatcga ggaaaaagt gtaaaactt gtaacacta attattaag ggttttaag cgtctacaa ttttgtaaa taagaattt gttttattt tagtacagg aaagtgtat tgttttatt caaatattc gttttttat aattgtaac tagagtgta ataacatgg ataataatg attaattcg tgctttgct ttccttttc ctgttgaat aatgattgg atatgtgat aatgtggat tttggcaca ataaaaacg atttgatat t
