Status
Please wait ...
Gene information
General information
TC001073 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10515700..10517969
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001073
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06761

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001073 RA
      MALHVPKAP GFAQMLKEG ARHYSGLEE AVIRNINAC 
      KEFAQSVRS AYGPNGMNK MVINHLEKQ FVTSDAATI 
      IRELDIEHP AAKLMILAS QMQDSEVGD GTNFVIILA 
      GALLEASEE LIRLGVTPT EVAEGYEKA LEKCLEILP 
      KLVCYEIKD YRNLNEVVK GIRTSIQSK QYGHEDFLA 
      NLVAKACVS ILPEDTTFN VDNVRVCKV LGSGLQSSQ 
      VVQGMVFKR HVEGEITKV QNAKVAVFS CAVDIMQTE 
      TKGTVLIKT ADELMNFSR GEENLLEIQ IKSIADTGA 
      KVIVAGAKF GDMALHYIH KYGLMAVRL NSKFDVRRL 
      SKTVGATVL PRLTAPTPQ ELGYCDVVC VEELGDTPI 
      VVFRLEGKE SRISTVVVR GATDNYMDD IERAIDDGV 
      NTFKGLSRD GRFLPGAGA VEAELAHQL TQYADTLPG 
      LEQYAVRKF ATALESFPK TLADNSGHR STVVLEKIL 
      QAHQNEQKN VGVDIDAES TICDALEKN ILDLYSCKY 
      WGLKYAVGA AATILRVDQ IIMAKRAGG PKPRQAAGS 
      DDES
    CDS sequence
    • >TC001073 RA
      atggctctt cacgtccct aaagccccg ggcttcgcc 
      cagatgttg aaagaaggc gcccggcac tactccggc 
      ctcgaagaa gccgtcatt cgaaatatt aacgcttgc 
      aaggaattt gcccagtcc gtgcgttcg gcttacggc 
      cccaatggc atgaacaag atggtgata aaccacctt 
      gagaagcag tttgtgacc agtgatgcc gccactatt 
      attagagaa ctggatatt gaacatccc gcggccaaa 
      ttaatgatt ctcgcgagt caaatgcaa gattccgag 
      gttggtgat ggcacaaac tttgtcatt atcctggct 
      ggggccctt ctggaggcc tcggaagag ctgatcaga 
      ctgggggtc acgccgacc gaagtggcc gagggctat 
      gagaaagcc ctggaaaag tgtctcgaa atcctcccg 
      aaactagtc tgttacgaa atcaaggat tacagaaac 
      ttgaatgag gtcgttaaa gggattaga acctcgatt 
      cagtcaaaa cagtacgga cacgaggac tttttggct 
      aatttagtg gccaaagcg tgtgtttca atcctgccc 
      gaggacacc acattcaac gttgacaat gtgcgtgtt 
      tgcaaggtg ctaggttcg ggtttgcaa agctcgcaa 
      gttgtccaa ggaatggtg tttaagaga cacgtcgag 
      ggcgaaatc actaaagtg cagaacgcc aaagttgct 
      gttttcagc tgtgcagtc gatatcatg caaactgag 
      accaaggga accgttttg atcaaaacc gcagatgag 
      ttgatgaat tttagtagg ggagaggag aatctcttg 
      gaaatacag atcaagtca attgccgac actggggct 
      aaggtgata gtggccggg gccaagttt ggggacatg 
      gccctgcac tacatccac aagtacgga ctgatggca 
      gtgcggctt aactcgaaa ttcgatgtg agaaggctc 
      agcaaaacg gttggagcg actgttttg cctcgtctg 
      accgccccc acccctcaa gaactgggc tactgcgat 
      gtggtctgt gttgaggaa ctcggggat acccccatt 
      gtggttttc aggcttgaa gggaaagag tcccgaatt 
      tccaccgtt gtcgttcgt ggggctact gataattat 
      atggacgat atcgaacga gcgattgac gatggtgtt 
      aacactttc aagggttta tcacgtgat gggcgtttc 
      ctgcctggg gcaggcgcc gttgaagcc gaattagcg 
      catcagttg actcaatat gctgatact cttcccggt 
      ttggaacaa tacgctgtc aggaagttt gcaacagct 
      ttggaaagt tttccgaag actcttgcc gacaacagc 
      gggcatcgg tctactgta gttttagaa aaaattctc 
      caagctcat cagaacgaa cagaaaaat gtcggcgtt 
      gatattgac gccgaaagc acgatttgc gacgccttg 
      gaaaagaac attttggac ttatactct tgcaaatac 
      tgggggctg aagtatgcc gtgggggct gctgctacc 
      attctccga gtggaccag ataatcatg gcgaagagg 
      gccggagga cccaaaccg agacaagca gcaggaagc 
      gatgacgaa tcataa
    mRNA sequence
    • >TC001073 RA
      tgttggtgg cgctgaacc caccaaatt agtcaccaa 
      aagttcaca tgtccattg ttgtgcaaa ctttttcga 
      ttattaata tttcgctta taaaaaaat aaatcggtc 
      tcaaaaatg gctcttcac gtccctaaa gccccgggc 
      ttcgcccag atgttgaaa gaaggcgcc cggcactac 
      tccggcctc gaagaagcc gtcattcga aatattaac 
      gcttgcaag gaatttgcc cagtccgtg cgttcggct 
      tacggcccc aatggcatg aacaagatg gtgataaac 
      caccttgag aagcagttt gtgaccagt gatgccgcc 
      actattatt agagaactg gatattgaa catcccgcg 
      gccaaatta atgattctc gcgagtcaa atgcaagat 
      tccgaggtt ggtgatggc acaaacttt gtcattatc 
      ctggctggg gcccttctg gaggcctcg gaagagctg 
      atcagactg ggggtcacg ccgaccgaa gtggccgag 
      ggctatgag aaagccctg gaaaagtgt ctcgaaatc 
      ctcccgaaa ctagtctgt tacgaaatc aaggattac 
      agaaacttg aatgaggtc gttaaaggg attagaacc 
      tcgattcag tcaaaacag tacggacac gaggacttt 
      ttggctaat ttagtggcc aaagcgtgt gtttcaatc 
      ctgcccgag gacaccaca ttcaacgtt gacaatgtg 
      cgtgtttgc aaggtgcta ggttcgggt ttgcaaagc 
      tcgcaagtt gtccaagga atggtgttt aagagacac 
      gtcgagggc gaaatcact aaagtgcag aacgccaaa 
      gttgctgtt ttcagctgt gcagtcgat atcatgcaa 
      actgagacc aagggaacc gttttgatc aaaaccgca 
      gatgagttg atgaatttt agtagggga gaggagaat 
      ctcttggaa atacagatc aagtcaatt gccgacact 
      ggggctaag gtgatagtg gccggggcc aagtttggg 
      gacatggcc ctgcactac atccacaag tacggactg 
      atggcagtg cggcttaac tcgaaattc gatgtgaga 
      aggctcagc aaaacggtt ggagcgact gttttgcct 
      cgtctgacc gcccccacc cctcaagaa ctgggctac 
      tgcgatgtg gtctgtgtt gaggaactc ggggatacc 
      cccattgtg gttttcagg cttgaaggg aaagagtcc 
      cgaatttcc accgttgtc gttcgtggg gctactgat 
      aattatatg gacgatatc gaacgagcg attgacgat 
      ggtgttaac actttcaag ggtttatca cgtgatggg 
      cgtttcctg cctggggca ggcgccgtt gaagccgaa 
      ttagcgcat cagttgact caatatgct gatactctt 
      cccggtttg gaacaatac gctgtcagg aagtttgca 
      acagctttg gaaagtttt ccgaagact cttgccgac 
      aacagcggg catcggtct actgtagtt ttagaaaaa 
      attctccaa gctcatcag aacgaacag aaaaatgtc 
      ggcgttgat attgacgcc gaaagcacg atttgcgac 
      gccttggaa aagaacatt ttggactta tactcttgc 
      aaatactgg gggctgaag tatgccgtg ggggctgct 
      gctaccatt ctccgagtg gaccagata atcatggcg 
      aagagggcc ggaggaccc aaaccgaga caagcagca 
      ggaagcgat gacgaatca taaatagtt tttttttaa 
      ttcgttaac aatacttta acgttttgt atttgcact 
      ttcacatgt attatttaa cagttatta ataaataac 
      aagtatcga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY