| General information | |||
| TC011309 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 3144274..3147816 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC011309 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06758 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC011309 RA
MEFSKAQIA ALVLGYLKS EKLYSVIEE FLKNCSYFN ENTQNIKMG KYFVTRVLG MRLVDFLEE YAMIYSIVQ GRLEETNYY ETHTRTSLV DQIMYLLDK ETATVATST PVISSSVGS EVDTTPAHT LPGNNSEEN EPAITKTPK KKSTRSKSR EKEEILDVS EVLTQTLLE NHELHEKIA ETINIVRNT PETGRAATE EHVSAGASK ELDALIKTV VQRTEEDPV YNKLIDEII GTAPYDNQS KKSTGSTHN NDDSSKPKS GDPMDAHNN DAVRSIIEV AQNTDPPPP EAPKPQNGG EVYVFDGTL DTYLKTTGA TPAVAPNQL YFNTGGLLI MPPEAPPKQ YYIVNNQPV INMGVPTVK NITENDIMA MPTIIVNDE SCTNLTNQT VAPKPPKRI VPKPEEPPP ATSPASKPA SHVRNLDFS TPGMRNNSS KTWDADLRS LVSGCANKT PTPKRKRVI VTKKKAKAK KKTEEEEPK AMQTPVKDT APKTPGPAP NTNDCTPFS ALLEEQLQG VDLISMPTP KIPITPGFA LTPGDLAPY NQRSTDYST SSSYYQPSD TEQNKSLDQ LIEECKRLE NNKSPQPPD KFTNLKILS DVVIEQAKV VTEPEKSQT TLKKFNQSV IGKKNLNLL NRHPTTSSS SDSDSDSDS ESPEHTVIK KTDVKQTDD TSSINNILN EVQVKSTFD EEKRKKDIL AQLEVVRKR TIDKIKKTT RKAPVRNNR KNAKTPKVP RKKPAKKQP VEEKVEDEE AQKLIHGLA ERGIHLVQN KTPQKVKEQ TLESDVSCP TFDRKIYQD VESVVHSDK TRKKALNKY NFEILKKTM SVQVFDELD REVTMMIKW TGFESIFEL NPRAKSTTP KKKLDDEED ELMDYVFLA DARDSSPEK VTKGGRKRK HKSSESEKQ TEKKAKAYE NNIKLLKKI DVDDFLDKV HGE
- >TC011309 RA
atggagttc agtaaagca caaatagcg gccttagtt ttaggttac ctcaaaagt gagaaatta tactcagtg attgaggag ttcctcaaa aactgttca tattttaat gaaaacacc caaaacatc aaaatgggg aagtatttt gttacccgt gtattggga atgcgtctt gttgatttc cttgaagag tatgctatg atttactcc atagttcaa ggccgtctc gaagagacc aattactac gaaacacat acaagaacc tcattggtg gaccaaatt atgtacttg ttggacaaa gaaacagcg actgtggcc acttcaacc cctgtgata agttcaagt gttggtagt gaagtagac accacaccg gcacacact ttaccagga aacaattcg gaagaaaat gaacccgca atcaccaaa acaccaaag aagaaatcg acaaggagc aaaagcaga gagaaagaa gagattttg gacgtttct gaagttttg acccaaact ttgttagaa aatcacgaa ttgcatgaa aaaatcgcg gaaactatc aacatagtc aggaatacg cctgaaacc ggtcgcgct gccacagag gaacacgtc agtgctggt gcgtctaag gaactagac gctttaatt aaaacagta gtacaaagg actgaagaa gacccagtg tacaataaa ctaattgat gagattatc ggaacggcg ccttacgac aaccaatca aaaaaatcc accggctcc acccacaat aacgacgat tcgtccaaa cccaagagt ggggaccca atggacgcc cacaacaac gacgctgtg cgttccata atcgaggtg gcacagaac acagacccg ccccctccc gaggcccca aagccccaa aatgggggc gaagtctac gttttcgac ggcacactt gacacatat ctgaaaacg accggcgcc acgcccgcg gtggcccca aaccagctc tacttcaac accgggggg ctcctaata atgcccccc gaagcccca ccaaagcaa tactacatc gtcaacaac caaccagtg ataaacatg ggcgtgcca acggtaaaa aacataaca gagaatgac atcatggca atgcctact ataatcgta aacgacgaa agttgtacc aaccttacg aaccaaaca gttgcgcca aaacccccc aaacgtatt gttcccaaa cccgaggag ccgccccct gccacaagc cccgcctcc aagcccgcc tcccacgta cgcaacctt gatttttca acgccggga atgcggaat aattcgagt aagacctgg gatgcggat ttgaggagt ttggtgtca ggttgtgcc aataaaacg cccactccg aagaggaaa cgggtgatt gtgacgaaa aagaaggcc aaagcgaag aaaaaaact gaagaggag gagcctaaa gctatgcaa actccggtc aaggacacc gccccgaaa acaccaggc cccgccccc aataccaac gattgtacc ccgttttcg gcgcttttg gaggagcaa ctccagggg gttgattta atttcaatg cccaccccc aaaattccg attacgccc ggatttgcc ctaactccg ggtgatttg gcgccttat aatcaacgc tcaaccgac tattcaacc tccagttcg tactaccaa ccttccgac acggaacaa aacaagtcc ctagaccag ttgatagaa gagtgcaag cgattggag aataataag tcgccacag ccgcctgat aaattcaca aatttgaaa atcctaagc gacgttgta atcgagcag gccaaggtt gtaaccgaa ccggaaaaa tcgcaaacc acgttaaag aaattcaac caaagtgtg atcggtaag aagaatttg aatcttttg aatcgccat cccacgact tcgagttcg tcggattcg gattcggat tcagattcg gaatcgccc gaacacacc gtaatcaaa aagactgat gttaagcaa accgacgat acgtctagt attaataat attttgaac gaggttcaa gtgaaaagt acgtttgat gaggagaag cgtaagaag gacattttg gcccaattg gaggtcgtc cggaaacga acaattgac aaaatcaaa aaaacaacc cggaaagct ccggttcgg aacaaccgg aaaaatgcg aaaaccccc aaagtgccg cggaaaaaa ccggcgaaa aaacagcca gttgaggag aaagtcgag gacgaagag gcgcaaaag ctgattcat ggcttggcc gagcggggg attcactta gttcagaat aaaacaccg caaaaagtt aaagaacag acgcttgaa tcggacgtt tcttgtccg acctttgat aggaaaatt taccaggat gtcgagtcc gtggttcat tcggacaag acccgaaag aaggctttg aacaagtac aattttgag attttgaaa aaaaccatg tccgtgcaa gttttcgac gagttggac agagaagtg acgatgatg attaagtgg acgggtttt gaatcgatt tttgagtta aatccaaga gctaagtcg acaacgccg aagaagaaa ctggacgat gaggaggat gagctcatg gattatgtt ttcttggcc gatgcaagg gacagttcg ccggaaaaa gtgacgaaa ggggggcgg aagaggaag cataaaagt agcgagagt gagaaacag acggagaag aaggcgaaa gcctacgaa aacaatatt aaacttttg aagaaaatt gacgttgat gattttctt gataaagtg catggagag tga
- >TC011309 RA
gtttttgaa attatttgt gccaaaact acagtaaaa ataatgaaa tgtttattt ttgaagtgt ttttaatac tctgtgtcc agaaattat taaaaaacc gttcgttta agttcaggc gtctctaaa tttagtggc atcagcaaa gtttgaggt taaactttt gcgtttttc ttttacata aacaattta ctcaaatta gtatttatt tttaatttt aatcaaata agtgtccaa acgccgata ttttgcaat aatggagtt cagtaaagc acaaatagc ggccttagt tttaggtta cctcaaaag tgagaaatt atactcagt gattgagga gttcctcaa aaactgttc atattttaa tgaaaacac ccaaaacat caaaatggg gaagtattt tgttacccg tgtattggg aatgcgtct tgttgattt ccttgaaga gtatgctat gatttactc catagttca aggccgtct cgaagagac caattacta cgaaacaca tacaagaac ctcattggt ggaccaaat tatgtactt gttggacaa agaaacagc gactgtggc cacttcaac ccctgtgat aagttcaag tgttggtag tgaagtaga caccacacc ggcacacac tttaccagg aaacaattc ggaagaaaa tgaacccgc aatcaccaa aacaccaaa gaagaaatc gacaaggag caaaagcag agagaaaga agagatttt ggacgtttc tgaagtttt gacccaaac tttgttaga aaatcacga attgcatga aaaaatcgc ggaaactat caacatagt caggaatac gcctgaaac cggtcgcgc tgccacaga ggaacacgt cagtgctgg tgcgtctaa ggaactaga cgctttaat taaaacagt agtacaaag gactgaaga agacccagt gtacaataa actaattga tgagattat cggaacggc gccttacga caaccaatc aaaaaaatc caccggctc cacccacaa taacgacga ttcgtccaa acccaagag tggggaccc aatggacgc ccacaacaa cgacgctgt gcgttccat aatcgaggt ggcacagaa cacagaccc gccccctcc cgaggcccc aaagcccca aaatggggg cgaagtcta cgttttcga cggcacact tgacacata tctgaaaac gaccggcgc cacgcccgc ggtggcccc aaaccagct ctacttcaa caccggggg gctcctaat aatgccccc cgaagcccc accaaagca atactacat cgtcaacaa ccaaccagt gataaacat gggcgtgcc aacggtaaa aaacataac agagaatga catcatggc aatgcctac tataatcgt aaacgacga aagttgtac caaccttac gaaccaaac agttgcgcc aaaaccccc caaacgtat tgttcccaa acccgagga gccgccccc tgccacaag ccccgcctc caagcccgc ctcccacgt acgcaacct tgatttttc aacgccggg aatgcggaa taattcgag taagacctg ggatgcgga tttgaggag tttggtgtc aggttgtgc caataaaac gcccactcc gaagaggaa acgggtgat tgtgacgaa aaagaaggc caaagcgaa gaaaaaaac tgaagagga ggagcctaa agctatgca aactccggt caaggacac cgccccgaa aacaccagg ccccgcccc caataccaa cgattgtac cccgttttc ggcgctttt ggaggagca actccaggg ggttgattt aatttcaat gcccacccc caaaattcc gattacgcc cggatttgc cctaactcc gggtgattt ggcgcctta taatcaacg ctcaaccga ctattcaac ctccagttc gtactacca accttccga cacggaaca aaacaagtc cctagacca gttgataga agagtgcaa gcgattgga gaataataa gtcgccaca gccgcctga taaattcac aaatttgaa aatcctaag cgacgttgt aatcgagca ggccaaggt tgtaaccga accggaaaa atcgcaaac cacgttaaa gaaattcaa ccaaagtgt gatcggtaa gaagaattt gaatctttt gaatcgcca tcccacgac ttcgagttc gtcggattc ggattcgga ttcagattc ggaatcgcc cgaacacac cgtaatcaa aaagactga tgttaagca aaccgacga tacgtctag tattaataa tattttgaa cgaggttca agtgaaaag tacgtttga tgaggagaa gcgtaagaa ggacatttt ggcccaatt ggaggtcgt ccggaaacg aacaattga caaaatcaa aaaaacaac ccggaaagc tccggttcg gaacaaccg gaaaaatgc gaaaacccc caaagtgcc gcggaaaaa accggcgaa aaaacagcc agttgagga gaaagtcga ggacgaaga ggcgcaaaa gctgattca tggcttggc cgagcgggg gattcactt agttcagaa taaaacacc gcaaaaagt taaagaaca gacgcttga atcggacgt ttcttgtcc gacctttga taggaaaat ttaccagga tgtcgagtc cgtggttca ttcggacaa gacccgaaa gaaggcttt gaacaagta caattttga gattttgaa aaaaaccat gtccgtgca agttttcga cgagttgga cagagaagt gacgatgat gattaagtg gacgggttt tgaatcgat ttttgagtt aaatccaag agctaagtc gacaacgcc gaagaagaa actggacga tgaggagga tgagctcat ggattatgt tttcttggc cgatgcaag ggacagttc gccggaaaa agtgacgaa aggggggcg gaagaggaa gcataaaag tagcgagag tgagaaaca gacggagaa gaaggcgaa agcctacga aaacaatat taaactttt gaagaaaat tgacgttga tgattttct tgataaagt gcatggaga gtgagtgag ttttgagcc tgagagttt cagttagtt tctagtttg gagtttaca gataagtac gatttttta ttgtacagt gtgtaaata agacgtttt tgaattttt cgatttttt atttggttt acaagtgat taaaaaata tttcatccc aaaaggaaa aacatacta gtctcaccc tgcatagta tgtaattaa tttcattaa tttattatt aagtattga gtagatcca gggctaggg ggacagccc ccaacttca aatttaatt tttttataa ataaaagcc taaagaaca a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
