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Gene information
General information
TC011309 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3144274..3147816
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011309
    iBeetle screen iB_06758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011309 RA
      MEFSKAQIA ALVLGYLKS EKLYSVIEE FLKNCSYFN 
ENTQNIKMG KYFVTRVLG MRLVDFLEE YAMIYSIVQ 
GRLEETNYY ETHTRTSLV DQIMYLLDK ETATVATST 
PVISSSVGS EVDTTPAHT LPGNNSEEN EPAITKTPK 
KKSTRSKSR EKEEILDVS EVLTQTLLE NHELHEKIA 
ETINIVRNT PETGRAATE EHVSAGASK ELDALIKTV 
VQRTEEDPV YNKLIDEII GTAPYDNQS KKSTGSTHN 
NDDSSKPKS GDPMDAHNN DAVRSIIEV AQNTDPPPP 
EAPKPQNGG EVYVFDGTL DTYLKTTGA TPAVAPNQL 
YFNTGGLLI MPPEAPPKQ YYIVNNQPV INMGVPTVK 
NITENDIMA MPTIIVNDE SCTNLTNQT VAPKPPKRI 
VPKPEEPPP ATSPASKPA SHVRNLDFS TPGMRNNSS 
KTWDADLRS LVSGCANKT PTPKRKRVI VTKKKAKAK 
KKTEEEEPK AMQTPVKDT APKTPGPAP NTNDCTPFS 
ALLEEQLQG VDLISMPTP KIPITPGFA LTPGDLAPY 
NQRSTDYST SSSYYQPSD TEQNKSLDQ LIEECKRLE 
NNKSPQPPD KFTNLKILS DVVIEQAKV VTEPEKSQT 
TLKKFNQSV IGKKNLNLL NRHPTTSSS SDSDSDSDS 
ESPEHTVIK KTDVKQTDD TSSINNILN EVQVKSTFD 
EEKRKKDIL AQLEVVRKR TIDKIKKTT RKAPVRNNR 
KNAKTPKVP RKKPAKKQP VEEKVEDEE AQKLIHGLA 
ERGIHLVQN KTPQKVKEQ TLESDVSCP TFDRKIYQD 
VESVVHSDK TRKKALNKY NFEILKKTM SVQVFDELD 
REVTMMIKW TGFESIFEL NPRAKSTTP KKKLDDEED 
ELMDYVFLA DARDSSPEK VTKGGRKRK HKSSESEKQ 
TEKKAKAYE NNIKLLKKI DVDDFLDKV HGE
    CDS sequence
    • >TC011309 RA
      atggagttc agtaaagca caaatagcg gccttagtt 
ttaggttac ctcaaaagt gagaaatta tactcagtg 
attgaggag ttcctcaaa aactgttca tattttaat 
gaaaacacc caaaacatc aaaatgggg aagtatttt 
gttacccgt gtattggga atgcgtctt gttgatttc 
cttgaagag tatgctatg atttactcc atagttcaa 
ggccgtctc gaagagacc aattactac gaaacacat 
acaagaacc tcattggtg gaccaaatt atgtacttg 
ttggacaaa gaaacagcg actgtggcc acttcaacc 
cctgtgata agttcaagt gttggtagt gaagtagac 
accacaccg gcacacact ttaccagga aacaattcg 
gaagaaaat gaacccgca atcaccaaa acaccaaag 
aagaaatcg acaaggagc aaaagcaga gagaaagaa 
gagattttg gacgtttct gaagttttg acccaaact 
ttgttagaa aatcacgaa ttgcatgaa aaaatcgcg 
gaaactatc aacatagtc aggaatacg cctgaaacc 
ggtcgcgct gccacagag gaacacgtc agtgctggt 
gcgtctaag gaactagac gctttaatt aaaacagta 
gtacaaagg actgaagaa gacccagtg tacaataaa 
ctaattgat gagattatc ggaacggcg ccttacgac 
aaccaatca aaaaaatcc accggctcc acccacaat 
aacgacgat tcgtccaaa cccaagagt ggggaccca 
atggacgcc cacaacaac gacgctgtg cgttccata 
atcgaggtg gcacagaac acagacccg ccccctccc 
gaggcccca aagccccaa aatgggggc gaagtctac 
gttttcgac ggcacactt gacacatat ctgaaaacg 
accggcgcc acgcccgcg gtggcccca aaccagctc 
tacttcaac accgggggg ctcctaata atgcccccc 
gaagcccca ccaaagcaa tactacatc gtcaacaac 
caaccagtg ataaacatg ggcgtgcca acggtaaaa 
aacataaca gagaatgac atcatggca atgcctact 
ataatcgta aacgacgaa agttgtacc aaccttacg 
aaccaaaca gttgcgcca aaacccccc aaacgtatt 
gttcccaaa cccgaggag ccgccccct gccacaagc 
cccgcctcc aagcccgcc tcccacgta cgcaacctt 
gatttttca acgccggga atgcggaat aattcgagt 
aagacctgg gatgcggat ttgaggagt ttggtgtca 
ggttgtgcc aataaaacg cccactccg aagaggaaa 
cgggtgatt gtgacgaaa aagaaggcc aaagcgaag 
aaaaaaact gaagaggag gagcctaaa gctatgcaa 
actccggtc aaggacacc gccccgaaa acaccaggc 
cccgccccc aataccaac gattgtacc ccgttttcg 
gcgcttttg gaggagcaa ctccagggg gttgattta 
atttcaatg cccaccccc aaaattccg attacgccc 
ggatttgcc ctaactccg ggtgatttg gcgccttat 
aatcaacgc tcaaccgac tattcaacc tccagttcg 
tactaccaa ccttccgac acggaacaa aacaagtcc 
ctagaccag ttgatagaa gagtgcaag cgattggag 
aataataag tcgccacag ccgcctgat aaattcaca 
aatttgaaa atcctaagc gacgttgta atcgagcag 
gccaaggtt gtaaccgaa ccggaaaaa tcgcaaacc 
acgttaaag aaattcaac caaagtgtg atcggtaag 
aagaatttg aatcttttg aatcgccat cccacgact 
tcgagttcg tcggattcg gattcggat tcagattcg 
gaatcgccc gaacacacc gtaatcaaa aagactgat 
gttaagcaa accgacgat acgtctagt attaataat 
attttgaac gaggttcaa gtgaaaagt acgtttgat 
gaggagaag cgtaagaag gacattttg gcccaattg 
gaggtcgtc cggaaacga acaattgac aaaatcaaa 
aaaacaacc cggaaagct ccggttcgg aacaaccgg 
aaaaatgcg aaaaccccc aaagtgccg cggaaaaaa 
ccggcgaaa aaacagcca gttgaggag aaagtcgag 
gacgaagag gcgcaaaag ctgattcat ggcttggcc 
gagcggggg attcactta gttcagaat aaaacaccg 
caaaaagtt aaagaacag acgcttgaa tcggacgtt 
tcttgtccg acctttgat aggaaaatt taccaggat 
gtcgagtcc gtggttcat tcggacaag acccgaaag 
aaggctttg aacaagtac aattttgag attttgaaa 
aaaaccatg tccgtgcaa gttttcgac gagttggac 
agagaagtg acgatgatg attaagtgg acgggtttt 
gaatcgatt tttgagtta aatccaaga gctaagtcg 
acaacgccg aagaagaaa ctggacgat gaggaggat 
gagctcatg gattatgtt ttcttggcc gatgcaagg 
gacagttcg ccggaaaaa gtgacgaaa ggggggcgg 
aagaggaag cataaaagt agcgagagt gagaaacag 
acggagaag aaggcgaaa gcctacgaa aacaatatt 
aaacttttg aagaaaatt gacgttgat gattttctt 
gataaagtg catggagag tga
    mRNA sequence
    • >TC011309 RA
      gtttttgaa attatttgt gccaaaact acagtaaaa 
ataatgaaa tgtttattt ttgaagtgt ttttaatac 
tctgtgtcc agaaattat taaaaaacc gttcgttta 
agttcaggc gtctctaaa tttagtggc atcagcaaa 
gtttgaggt taaactttt gcgtttttc ttttacata 
aacaattta ctcaaatta gtatttatt tttaatttt 
aatcaaata agtgtccaa acgccgata ttttgcaat 
aatggagtt cagtaaagc acaaatagc ggccttagt 
tttaggtta cctcaaaag tgagaaatt atactcagt 
gattgagga gttcctcaa aaactgttc atattttaa 
tgaaaacac ccaaaacat caaaatggg gaagtattt 
tgttacccg tgtattggg aatgcgtct tgttgattt 
ccttgaaga gtatgctat gatttactc catagttca 
aggccgtct cgaagagac caattacta cgaaacaca 
tacaagaac ctcattggt ggaccaaat tatgtactt 
gttggacaa agaaacagc gactgtggc cacttcaac 
ccctgtgat aagttcaag tgttggtag tgaagtaga 
caccacacc ggcacacac tttaccagg aaacaattc 
ggaagaaaa tgaacccgc aatcaccaa aacaccaaa 
gaagaaatc gacaaggag caaaagcag agagaaaga 
agagatttt ggacgtttc tgaagtttt gacccaaac 
tttgttaga aaatcacga attgcatga aaaaatcgc 
ggaaactat caacatagt caggaatac gcctgaaac 
cggtcgcgc tgccacaga ggaacacgt cagtgctgg 
tgcgtctaa ggaactaga cgctttaat taaaacagt 
agtacaaag gactgaaga agacccagt gtacaataa 
actaattga tgagattat cggaacggc gccttacga 
caaccaatc aaaaaaatc caccggctc cacccacaa 
taacgacga ttcgtccaa acccaagag tggggaccc 
aatggacgc ccacaacaa cgacgctgt gcgttccat 
aatcgaggt ggcacagaa cacagaccc gccccctcc 
cgaggcccc aaagcccca aaatggggg cgaagtcta 
cgttttcga cggcacact tgacacata tctgaaaac 
gaccggcgc cacgcccgc ggtggcccc aaaccagct 
ctacttcaa caccggggg gctcctaat aatgccccc 
cgaagcccc accaaagca atactacat cgtcaacaa 
ccaaccagt gataaacat gggcgtgcc aacggtaaa 
aaacataac agagaatga catcatggc aatgcctac 
tataatcgt aaacgacga aagttgtac caaccttac 
gaaccaaac agttgcgcc aaaaccccc caaacgtat 
tgttcccaa acccgagga gccgccccc tgccacaag 
ccccgcctc caagcccgc ctcccacgt acgcaacct 
tgatttttc aacgccggg aatgcggaa taattcgag 
taagacctg ggatgcgga tttgaggag tttggtgtc 
aggttgtgc caataaaac gcccactcc gaagaggaa 
acgggtgat tgtgacgaa aaagaaggc caaagcgaa 
gaaaaaaac tgaagagga ggagcctaa agctatgca 
aactccggt caaggacac cgccccgaa aacaccagg 
ccccgcccc caataccaa cgattgtac cccgttttc 
ggcgctttt ggaggagca actccaggg ggttgattt 
aatttcaat gcccacccc caaaattcc gattacgcc 
cggatttgc cctaactcc gggtgattt ggcgcctta 
taatcaacg ctcaaccga ctattcaac ctccagttc 
gtactacca accttccga cacggaaca aaacaagtc 
cctagacca gttgataga agagtgcaa gcgattgga 
gaataataa gtcgccaca gccgcctga taaattcac 
aaatttgaa aatcctaag cgacgttgt aatcgagca 
ggccaaggt tgtaaccga accggaaaa atcgcaaac 
cacgttaaa gaaattcaa ccaaagtgt gatcggtaa 
gaagaattt gaatctttt gaatcgcca tcccacgac 
ttcgagttc gtcggattc ggattcgga ttcagattc 
ggaatcgcc cgaacacac cgtaatcaa aaagactga 
tgttaagca aaccgacga tacgtctag tattaataa 
tattttgaa cgaggttca agtgaaaag tacgtttga 
tgaggagaa gcgtaagaa ggacatttt ggcccaatt 
ggaggtcgt ccggaaacg aacaattga caaaatcaa 
aaaaacaac ccggaaagc tccggttcg gaacaaccg 
gaaaaatgc gaaaacccc caaagtgcc gcggaaaaa 
accggcgaa aaaacagcc agttgagga gaaagtcga 
ggacgaaga ggcgcaaaa gctgattca tggcttggc 
cgagcgggg gattcactt agttcagaa taaaacacc 
gcaaaaagt taaagaaca gacgcttga atcggacgt 
ttcttgtcc gacctttga taggaaaat ttaccagga 
tgtcgagtc cgtggttca ttcggacaa gacccgaaa 
gaaggcttt gaacaagta caattttga gattttgaa 
aaaaaccat gtccgtgca agttttcga cgagttgga 
cagagaagt gacgatgat gattaagtg gacgggttt 
tgaatcgat ttttgagtt aaatccaag agctaagtc 
gacaacgcc gaagaagaa actggacga tgaggagga 
tgagctcat ggattatgt tttcttggc cgatgcaag 
ggacagttc gccggaaaa agtgacgaa aggggggcg 
gaagaggaa gcataaaag tagcgagag tgagaaaca 
gacggagaa gaaggcgaa agcctacga aaacaatat 
taaactttt gaagaaaat tgacgttga tgattttct 
tgataaagt gcatggaga gtgagtgag ttttgagcc 
tgagagttt cagttagtt tctagtttg gagtttaca 
gataagtac gatttttta ttgtacagt gtgtaaata 
agacgtttt tgaattttt cgatttttt atttggttt 
acaagtgat taaaaaata tttcatccc aaaaggaaa 
aacatacta gtctcaccc tgcatagta tgtaattaa 
tttcattaa tttattatt aagtattga gtagatcca 
gggctaggg ggacagccc ccaacttca aatttaatt 
tttttataa ataaaagcc taaagaaca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY