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Gene information
General information
TC011309 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3144274..3147816
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011309
    iBeetle screen iB_06758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011309 RA
      MEFSKAQIA ALVLGYLKS EKLYSVIEE FLKNCSYFN 
      ENTQNIKMG KYFVTRVLG MRLVDFLEE YAMIYSIVQ 
      GRLEETNYY ETHTRTSLV DQIMYLLDK ETATVATST 
      PVISSSVGS EVDTTPAHT LPGNNSEEN EPAITKTPK 
      KKSTRSKSR EKEEILDVS EVLTQTLLE NHELHEKIA 
      ETINIVRNT PETGRAATE EHVSAGASK ELDALIKTV 
      VQRTEEDPV YNKLIDEII GTAPYDNQS KKSTGSTHN 
      NDDSSKPKS GDPMDAHNN DAVRSIIEV AQNTDPPPP 
      EAPKPQNGG EVYVFDGTL DTYLKTTGA TPAVAPNQL 
      YFNTGGLLI MPPEAPPKQ YYIVNNQPV INMGVPTVK 
      NITENDIMA MPTIIVNDE SCTNLTNQT VAPKPPKRI 
      VPKPEEPPP ATSPASKPA SHVRNLDFS TPGMRNNSS 
      KTWDADLRS LVSGCANKT PTPKRKRVI VTKKKAKAK 
      KKTEEEEPK AMQTPVKDT APKTPGPAP NTNDCTPFS 
      ALLEEQLQG VDLISMPTP KIPITPGFA LTPGDLAPY 
      NQRSTDYST SSSYYQPSD TEQNKSLDQ LIEECKRLE 
      NNKSPQPPD KFTNLKILS DVVIEQAKV VTEPEKSQT 
      TLKKFNQSV IGKKNLNLL NRHPTTSSS SDSDSDSDS 
      ESPEHTVIK KTDVKQTDD TSSINNILN EVQVKSTFD 
      EEKRKKDIL AQLEVVRKR TIDKIKKTT RKAPVRNNR 
      KNAKTPKVP RKKPAKKQP VEEKVEDEE AQKLIHGLA 
      ERGIHLVQN KTPQKVKEQ TLESDVSCP TFDRKIYQD 
      VESVVHSDK TRKKALNKY NFEILKKTM SVQVFDELD 
      REVTMMIKW TGFESIFEL NPRAKSTTP KKKLDDEED 
      ELMDYVFLA DARDSSPEK VTKGGRKRK HKSSESEKQ 
      TEKKAKAYE NNIKLLKKI DVDDFLDKV HGE
    CDS sequence
    • >TC011309 RA
      atggagttc agtaaagca caaatagcg gccttagtt 
      ttaggttac ctcaaaagt gagaaatta tactcagtg 
      attgaggag ttcctcaaa aactgttca tattttaat 
      gaaaacacc caaaacatc aaaatgggg aagtatttt 
      gttacccgt gtattggga atgcgtctt gttgatttc 
      cttgaagag tatgctatg atttactcc atagttcaa 
      ggccgtctc gaagagacc aattactac gaaacacat 
      acaagaacc tcattggtg gaccaaatt atgtacttg 
      ttggacaaa gaaacagcg actgtggcc acttcaacc 
      cctgtgata agttcaagt gttggtagt gaagtagac 
      accacaccg gcacacact ttaccagga aacaattcg 
      gaagaaaat gaacccgca atcaccaaa acaccaaag 
      aagaaatcg acaaggagc aaaagcaga gagaaagaa 
      gagattttg gacgtttct gaagttttg acccaaact 
      ttgttagaa aatcacgaa ttgcatgaa aaaatcgcg 
      gaaactatc aacatagtc aggaatacg cctgaaacc 
      ggtcgcgct gccacagag gaacacgtc agtgctggt 
      gcgtctaag gaactagac gctttaatt aaaacagta 
      gtacaaagg actgaagaa gacccagtg tacaataaa 
      ctaattgat gagattatc ggaacggcg ccttacgac 
      aaccaatca aaaaaatcc accggctcc acccacaat 
      aacgacgat tcgtccaaa cccaagagt ggggaccca 
      atggacgcc cacaacaac gacgctgtg cgttccata 
      atcgaggtg gcacagaac acagacccg ccccctccc 
      gaggcccca aagccccaa aatgggggc gaagtctac 
      gttttcgac ggcacactt gacacatat ctgaaaacg 
      accggcgcc acgcccgcg gtggcccca aaccagctc 
      tacttcaac accgggggg ctcctaata atgcccccc 
      gaagcccca ccaaagcaa tactacatc gtcaacaac 
      caaccagtg ataaacatg ggcgtgcca acggtaaaa 
      aacataaca gagaatgac atcatggca atgcctact 
      ataatcgta aacgacgaa agttgtacc aaccttacg 
      aaccaaaca gttgcgcca aaacccccc aaacgtatt 
      gttcccaaa cccgaggag ccgccccct gccacaagc 
      cccgcctcc aagcccgcc tcccacgta cgcaacctt 
      gatttttca acgccggga atgcggaat aattcgagt 
      aagacctgg gatgcggat ttgaggagt ttggtgtca 
      ggttgtgcc aataaaacg cccactccg aagaggaaa 
      cgggtgatt gtgacgaaa aagaaggcc aaagcgaag 
      aaaaaaact gaagaggag gagcctaaa gctatgcaa 
      actccggtc aaggacacc gccccgaaa acaccaggc 
      cccgccccc aataccaac gattgtacc ccgttttcg 
      gcgcttttg gaggagcaa ctccagggg gttgattta 
      atttcaatg cccaccccc aaaattccg attacgccc 
      ggatttgcc ctaactccg ggtgatttg gcgccttat 
      aatcaacgc tcaaccgac tattcaacc tccagttcg 
      tactaccaa ccttccgac acggaacaa aacaagtcc 
      ctagaccag ttgatagaa gagtgcaag cgattggag 
      aataataag tcgccacag ccgcctgat aaattcaca 
      aatttgaaa atcctaagc gacgttgta atcgagcag 
      gccaaggtt gtaaccgaa ccggaaaaa tcgcaaacc 
      acgttaaag aaattcaac caaagtgtg atcggtaag 
      aagaatttg aatcttttg aatcgccat cccacgact 
      tcgagttcg tcggattcg gattcggat tcagattcg 
      gaatcgccc gaacacacc gtaatcaaa aagactgat 
      gttaagcaa accgacgat acgtctagt attaataat 
      attttgaac gaggttcaa gtgaaaagt acgtttgat 
      gaggagaag cgtaagaag gacattttg gcccaattg 
      gaggtcgtc cggaaacga acaattgac aaaatcaaa 
      aaaacaacc cggaaagct ccggttcgg aacaaccgg 
      aaaaatgcg aaaaccccc aaagtgccg cggaaaaaa 
      ccggcgaaa aaacagcca gttgaggag aaagtcgag 
      gacgaagag gcgcaaaag ctgattcat ggcttggcc 
      gagcggggg attcactta gttcagaat aaaacaccg 
      caaaaagtt aaagaacag acgcttgaa tcggacgtt 
      tcttgtccg acctttgat aggaaaatt taccaggat 
      gtcgagtcc gtggttcat tcggacaag acccgaaag 
      aaggctttg aacaagtac aattttgag attttgaaa 
      aaaaccatg tccgtgcaa gttttcgac gagttggac 
      agagaagtg acgatgatg attaagtgg acgggtttt 
      gaatcgatt tttgagtta aatccaaga gctaagtcg 
      acaacgccg aagaagaaa ctggacgat gaggaggat 
      gagctcatg gattatgtt ttcttggcc gatgcaagg 
      gacagttcg ccggaaaaa gtgacgaaa ggggggcgg 
      aagaggaag cataaaagt agcgagagt gagaaacag 
      acggagaag aaggcgaaa gcctacgaa aacaatatt 
      aaacttttg aagaaaatt gacgttgat gattttctt 
      gataaagtg catggagag tga
    mRNA sequence
    • >TC011309 RA
      gtttttgaa attatttgt gccaaaact acagtaaaa 
      ataatgaaa tgtttattt ttgaagtgt ttttaatac 
      tctgtgtcc agaaattat taaaaaacc gttcgttta 
      agttcaggc gtctctaaa tttagtggc atcagcaaa 
      gtttgaggt taaactttt gcgtttttc ttttacata 
      aacaattta ctcaaatta gtatttatt tttaatttt 
      aatcaaata agtgtccaa acgccgata ttttgcaat 
      aatggagtt cagtaaagc acaaatagc ggccttagt 
      tttaggtta cctcaaaag tgagaaatt atactcagt 
      gattgagga gttcctcaa aaactgttc atattttaa 
      tgaaaacac ccaaaacat caaaatggg gaagtattt 
      tgttacccg tgtattggg aatgcgtct tgttgattt 
      ccttgaaga gtatgctat gatttactc catagttca 
      aggccgtct cgaagagac caattacta cgaaacaca 
      tacaagaac ctcattggt ggaccaaat tatgtactt 
      gttggacaa agaaacagc gactgtggc cacttcaac 
      ccctgtgat aagttcaag tgttggtag tgaagtaga 
      caccacacc ggcacacac tttaccagg aaacaattc 
      ggaagaaaa tgaacccgc aatcaccaa aacaccaaa 
      gaagaaatc gacaaggag caaaagcag agagaaaga 
      agagatttt ggacgtttc tgaagtttt gacccaaac 
      tttgttaga aaatcacga attgcatga aaaaatcgc 
      ggaaactat caacatagt caggaatac gcctgaaac 
      cggtcgcgc tgccacaga ggaacacgt cagtgctgg 
      tgcgtctaa ggaactaga cgctttaat taaaacagt 
      agtacaaag gactgaaga agacccagt gtacaataa 
      actaattga tgagattat cggaacggc gccttacga 
      caaccaatc aaaaaaatc caccggctc cacccacaa 
      taacgacga ttcgtccaa acccaagag tggggaccc 
      aatggacgc ccacaacaa cgacgctgt gcgttccat 
      aatcgaggt ggcacagaa cacagaccc gccccctcc 
      cgaggcccc aaagcccca aaatggggg cgaagtcta 
      cgttttcga cggcacact tgacacata tctgaaaac 
      gaccggcgc cacgcccgc ggtggcccc aaaccagct 
      ctacttcaa caccggggg gctcctaat aatgccccc 
      cgaagcccc accaaagca atactacat cgtcaacaa 
      ccaaccagt gataaacat gggcgtgcc aacggtaaa 
      aaacataac agagaatga catcatggc aatgcctac 
      tataatcgt aaacgacga aagttgtac caaccttac 
      gaaccaaac agttgcgcc aaaaccccc caaacgtat 
      tgttcccaa acccgagga gccgccccc tgccacaag 
      ccccgcctc caagcccgc ctcccacgt acgcaacct 
      tgatttttc aacgccggg aatgcggaa taattcgag 
      taagacctg ggatgcgga tttgaggag tttggtgtc 
      aggttgtgc caataaaac gcccactcc gaagaggaa 
      acgggtgat tgtgacgaa aaagaaggc caaagcgaa 
      gaaaaaaac tgaagagga ggagcctaa agctatgca 
      aactccggt caaggacac cgccccgaa aacaccagg 
      ccccgcccc caataccaa cgattgtac cccgttttc 
      ggcgctttt ggaggagca actccaggg ggttgattt 
      aatttcaat gcccacccc caaaattcc gattacgcc 
      cggatttgc cctaactcc gggtgattt ggcgcctta 
      taatcaacg ctcaaccga ctattcaac ctccagttc 
      gtactacca accttccga cacggaaca aaacaagtc 
      cctagacca gttgataga agagtgcaa gcgattgga 
      gaataataa gtcgccaca gccgcctga taaattcac 
      aaatttgaa aatcctaag cgacgttgt aatcgagca 
      ggccaaggt tgtaaccga accggaaaa atcgcaaac 
      cacgttaaa gaaattcaa ccaaagtgt gatcggtaa 
      gaagaattt gaatctttt gaatcgcca tcccacgac 
      ttcgagttc gtcggattc ggattcgga ttcagattc 
      ggaatcgcc cgaacacac cgtaatcaa aaagactga 
      tgttaagca aaccgacga tacgtctag tattaataa 
      tattttgaa cgaggttca agtgaaaag tacgtttga 
      tgaggagaa gcgtaagaa ggacatttt ggcccaatt 
      ggaggtcgt ccggaaacg aacaattga caaaatcaa 
      aaaaacaac ccggaaagc tccggttcg gaacaaccg 
      gaaaaatgc gaaaacccc caaagtgcc gcggaaaaa 
      accggcgaa aaaacagcc agttgagga gaaagtcga 
      ggacgaaga ggcgcaaaa gctgattca tggcttggc 
      cgagcgggg gattcactt agttcagaa taaaacacc 
      gcaaaaagt taaagaaca gacgcttga atcggacgt 
      ttcttgtcc gacctttga taggaaaat ttaccagga 
      tgtcgagtc cgtggttca ttcggacaa gacccgaaa 
      gaaggcttt gaacaagta caattttga gattttgaa 
      aaaaaccat gtccgtgca agttttcga cgagttgga 
      cagagaagt gacgatgat gattaagtg gacgggttt 
      tgaatcgat ttttgagtt aaatccaag agctaagtc 
      gacaacgcc gaagaagaa actggacga tgaggagga 
      tgagctcat ggattatgt tttcttggc cgatgcaag 
      ggacagttc gccggaaaa agtgacgaa aggggggcg 
      gaagaggaa gcataaaag tagcgagag tgagaaaca 
      gacggagaa gaaggcgaa agcctacga aaacaatat 
      taaactttt gaagaaaat tgacgttga tgattttct 
      tgataaagt gcatggaga gtgagtgag ttttgagcc 
      tgagagttt cagttagtt tctagtttg gagtttaca 
      gataagtac gatttttta ttgtacagt gtgtaaata 
      agacgtttt tgaattttt cgatttttt atttggttt 
      acaagtgat taaaaaata tttcatccc aaaaggaaa 
      aacatacta gtctcaccc tgcatagta tgtaattaa 
      tttcattaa tttattatt aagtattga gtagatcca 
      gggctaggg ggacagccc ccaacttca aatttaatt 
      tttttataa ataaaagcc taaagaaca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY