Status
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Gene information
General information
TC004254 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450217.1: 0..36546
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004254
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06751

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004254 RA
        LNGRLTLKK LCGVADKVV ERQCNSFWR SGEQARSRL 
        RSLNPDKSS NCDLFGEIN CAPRDQDLA RSQPKSSVV 
        SSTPMPFSR HKNFLPNAS NSVSPITVV KPGNVTKPA 
        KASTSANSR TKLFKAEIS TPITDTSIR SQRLENIVL 
        SLHRSLRAE PVVNNQRLD KNASTMSRK NANIFETTD 
        KMMQTSNNN SLTGAKKLA RSRRSNCDL SFSRNEELH 
        FEKMVAEKR LADLIDKLE DNFLKRPPV ESRMEENFS 
        RIGTGVPWR VTIVPHENV ALNANTLLA FGSSDEESF 
        DDTFLPKMR PKPKVLVNK KKLKRGSGR FLKNATKNA 
        KQSKLKLDE RSMTSSVAS EVPQTADQG RMKSRAKGK 
        EKERVETLI KEPEENPGV KNKKRQSRS GTKSQTKHN 
        VLDANHFQE VTSNSELFT ERFLNEENK KVESTRKRQ 
        KKRDRTESP LKKLRKSDI NDSKSGKQN MTQDDGEMV 
        SYGTNTDNW RNDLFGESS MKEMEHLEK GNKSGQSLK 
        KEKKKQNAS PSKKQKAIV KCFANKNKE HSVTDDSGF 
        DESQRVSVK AVVHARATQ KNSKKPAGT QKQQLRNVD 
        NIHILEDVM TNSDEETSR KVEIFFESN NQDTNFGAP 
        NLDNLQQLR TITKKPSLN NSVNKSAGK STARKTQNK 
        EKKKQTKHS TTKSKAQST TGITETTTD ETTGCMQRR 
        SGRQRKQTK IYSAGYILC TERDRYYKV ATAVRDISQ 
        VTPECSFRL SGLQKRRTR ITQMPPIME PEVEMEGNT 
        QKSKRKTKK PAKKQSSKK LKKDETPKR QETRTVSES 
        ETNLTRSDY IVGENVITS DYVSAENVA QNTQTPFVR 
        LEEVTSRNI GSEELHNTV FSSPPFQAQ ESIKKNSVI 
        SGRISKRSS TKTSAKKTR DSLKKVVET QRDSSGNFM 
        ICPATNELI RYSFKNLVY KPSANLPGV MYAVTEDLK 
        LGWLKISGG MSKPLFKTK NHTMSFVVV EGKAEVGIQ 
        DKCDLYKKH DIFIVPQGA EYYINNKNK TNPLLLVFF 
        KVAYADKPT
      CDS sequence
      • >TC004254 RA
        ctcaatggt cgccttaca ctcaaaaaa ctctgcggg 
        gtcgctgat aaggtcgta gaaagacag tgtaatagt 
        ttctggaga agtggagaa caagccaga tctaggtta 
        agaagctta aatccagat aaaagtagc aactgtgat 
        ttatttggc gaaataaac tgcgcccct cgggaccaa 
        gacctggct cgctcgcaa ccaaaatct tcagttgtg 
        agcagtacc ccaatgcca tttagtcgg cacaaaaac 
        tttcttccg aatgccagt aattcagtg tcacccatt 
        accgtcgtc aaaccgggc aacgtaacc aaaccggcg 
        aaagcgagc acatcagcg aactcacgt accaaactc 
        ttcaaagca gaaatatcg acaccgatc acggacact 
        tcgatcaga agccagcgc ttggaaaac attgtgttg 
        tcgttacac cggtctctt cgcgccgaa cctgtggtc 
        aacaaccag cgcttggat aaaaatgcc tcgacaatg 
        tctcggaaa aacgcaaat attttcgaa accactgat 
        aaaatgatg caaacttcc aataataat tcattaacg 
        ggggcgaaa aagctggca aggagcagg agatccaac 
        tgtgatctt tcattctct aggaatgaa gaactccat 
        tttgagaaa atggtcgcg gagaagagg ctcgcagat 
        ttgatcgac aagttagaa gacaatttt ttgaagaga 
        ccgcctgtg gaatcccga atggaggag aatttttcg 
        agaattggt actggagta ccatggcga gtgacgatt 
        gtacctcat gaaaatgtt gcgttaaat gcaaatact 
        ttactagcg tttggaagt tccgatgag gagtctttt 
        gatgacact ttcctacca aaaatgcgc ccgaaaccc 
        aaagttctc gttaataaa aagaagttg aaaaggggt 
        tcgggaaga tttttgaag aatgcaacg aaaaacgca 
        aagcagagt aaactaaag ctggatgaa agaagtatg 
        actagttca gttgcaagt gaagtacct caaacggcg 
        gaccaagga cggatgaaa agtagggca aagggaaag 
        gaaaaagaa agggttgaa actttaata aaggaacca 
        gaagagaat ccaggagtt aaaaataaa aagagacaa 
        agccgaagt ggaactaaa tcacagacg aaacataac 
        gtactagac gctaatcat tttcaagaa gtgacgtct 
        aatagtgaa ctattcact gaacgtttt ttgaacgag 
        gaaaataaa aaagttgaa agtacacgg aaaagacag 
        aagaaacgg gataggact gaaagtccg ctcaaaaaa 
        ctaagaaaa tcagatata aacgattcc aagagtggc 
        aagcaaaat atgacacaa gacgatgga gaaatggtg 
        tcatatggt acaaatacg gataactgg agaaatgac 
        ttatttggt gaaagttcg atgaaagaa atggaacat 
        ttggagaag ggaaataaa tcagggcaa agtttaaag 
        aaagaaaaa aagaaacag aacgcaagt ccatcaaaa 
        aaacaaaaa gcaatagta aagtgtttt gcaaataaa 
        aataaggaa cacagtgtc actgatgac agtggtttc 
        gatgaatcg caaagagtg tcagttaag gcggtcgta 
        catgcaaga gctacacaa aaaaacagt aaaaagcca 
        gcaggaaca caaaaacaa caattaaga aatgtagac 
        aatatacat attttggaa gacgtaatg actaatagt 
        gatgaagaa acgtcacgc aaagttgaa atatttttt 
        gaatcaaat aatcaggat actaatttc ggtgcaccg 
        aatctggac aatttgcaa cagttacga actattacc 
        aagaaacca tcactaaat aacagcgtt aataaatca 
        gctggaaaa agtacagcg aggaaaaca caaaataaa 
        gagaaaaaa aagcagaca aaacattct acaacgaag 
        tctaaagcc caaagtaca acaggaatt actgaaacg 
        acaactgac gagacaaca ggatgtatg caaaggaga 
        tcgggaagg caacgaaaa caaacaaag atttattca 
        gctggttac atcctatgc actgaaagg gacagatat 
        tataaagtc gcaacggcg gtaagggat atatcacaa 
        gtaacgcct gaatgttca tttcgactt tcgggacta 
        caaaaacgg cgtactcgt ataactcaa atgccacca 
        attatggag ccggaagtt gaaatggaa ggtaatact 
        caaaagtcg aaaaggaaa actaagaaa ccggctaaa 
        aaacaatca agtaagaaa ctaaagaaa gatgagacc 
        ccaaaacgt caagaaacc agaactgtt tctgaatcg 
        gagacaaat ctaaccaga agtgattac attgttgga 
        gaaaatgta attacctcc gattatgtt agtgcagaa 
        aatgttgca caaaacact caaacacca tttgtaaga 
        ctagaagaa gtaacttca aggaacatt ggttctgaa 
        gaacttcat aacaccgtc ttttcttca ccaccgttc 
        caagcacaa gagagtatc aagaaaaat tctgttata 
        tctggaaga atatctaaa cggtctagt accaaaacc 
        agcgcgaaa aaaactagg gattccttg aaaaaggta 
        gtcgagact caacgcgac tcaagcggt aatttcatg 
        atctgtcca gcaactaat gagcttatt agatacagt 
        ttcaaaaat ctggtgtac aaaccgagc gctaatttg 
        cctggtgtc atgtacgct gtgacggaa gatctaaaa 
        ttgggttgg ttgaagata agcggagga atgtcaaag 
        ccattgttt aaaactaag aatcacact atgtcgttc 
        gtagttgta gaaggaaaa gcggaggtg gggatacag 
        gacaagtgt gatttgtat aaaaaacac gacatattt 
        attgttcca caaggagcg gaatattac ataaataac 
        aaaaataaa actaacccc ttgctattg gttttcttc 
        aaagtcgca tatgcggac aaaccgacg taa
      mRNA sequence
      • >TC004254 RA
        ctcaatggt cgccttaca ctcaaaaaa ctctgcggg 
        gtcgctgat aaggtcgta gaaagacag tgtaatagt 
        ttctggaga agtggagaa caagccaga tctaggtta 
        agaagctta aatccagat aaaagtagc aactgtgat 
        ttatttggc gaaataaac tgcgcccct cgggaccaa 
        gacctggct cgctcgcaa ccaaaatct tcagttgtg 
        agcagtacc ccaatgcca tttagtcgg cacaaaaac 
        tttcttccg aatgccagt aattcagtg tcacccatt 
        accgtcgtc aaaccgggc aacgtaacc aaaccggcg 
        aaagcgagc acatcagcg aactcacgt accaaactc 
        ttcaaagca gaaatatcg acaccgatc acggacact 
        tcgatcaga agccagcgc ttggaaaac attgtgttg 
        tcgttacac cggtctctt cgcgccgaa cctgtggtc 
        aacaaccag cgcttggat aaaaatgcc tcgacaatg 
        tctcggaaa aacgcaaat attttcgaa accactgat 
        aaaatgatg caaacttcc aataataat tcattaacg 
        ggggcgaaa aagctggca aggagcagg agatccaac 
        tgtgatctt tcattctct aggaatgaa gaactccat 
        tttgagaaa atggtcgcg gagaagagg ctcgcagat 
        ttgatcgac aagttagaa gacaatttt ttgaagaga 
        ccgcctgtg gaatcccga atggaggag aatttttcg 
        agaattggt actggagta ccatggcga gtgacgatt 
        gtacctcat gaaaatgtt gcgttaaat gcaaatact 
        ttactagcg tttggaagt tccgatgag gagtctttt 
        gatgacact ttcctacca aaaatgcgc ccgaaaccc 
        aaagttctc gttaataaa aagaagttg aaaaggggt 
        tcgggaaga tttttgaag aatgcaacg aaaaacgca 
        aagcagagt aaactaaag ctggatgaa agaagtatg 
        actagttca gttgcaagt gaagtacct caaacggcg 
        gaccaagga cggatgaaa agtagggca aagggaaag 
        gaaaaagaa agggttgaa actttaata aaggaacca 
        gaagagaat ccaggagtt aaaaataaa aagagacaa 
        agccgaagt ggaactaaa tcacagacg aaacataac 
        gtactagac gctaatcat tttcaagaa gtgacgtct 
        aatagtgaa ctattcact gaacgtttt ttgaacgag 
        gaaaataaa aaagttgaa agtacacgg aaaagacag 
        aagaaacgg gataggact gaaagtccg ctcaaaaaa 
        ctaagaaaa tcagatata aacgattcc aagagtggc 
        aagcaaaat atgacacaa gacgatgga gaaatggtg 
        tcatatggt acaaatacg gataactgg agaaatgac 
        ttatttggt gaaagttcg atgaaagaa atggaacat 
        ttggagaag ggaaataaa tcagggcaa agtttaaag 
        aaagaaaaa aagaaacag aacgcaagt ccatcaaaa 
        aaacaaaaa gcaatagta aagtgtttt gcaaataaa 
        aataaggaa cacagtgtc actgatgac agtggtttc 
        gatgaatcg caaagagtg tcagttaag gcggtcgta 
        catgcaaga gctacacaa aaaaacagt aaaaagcca 
        gcaggaaca caaaaacaa caattaaga aatgtagac 
        aatatacat attttggaa gacgtaatg actaatagt 
        gatgaagaa acgtcacgc aaagttgaa atatttttt 
        gaatcaaat aatcaggat actaatttc ggtgcaccg 
        aatctggac aatttgcaa cagttacga actattacc 
        aagaaacca tcactaaat aacagcgtt aataaatca 
        gctggaaaa agtacagcg aggaaaaca caaaataaa 
        gagaaaaaa aagcagaca aaacattct acaacgaag 
        tctaaagcc caaagtaca acaggaatt actgaaacg 
        acaactgac gagacaaca ggatgtatg caaaggaga 
        tcgggaagg caacgaaaa caaacaaag atttattca 
        gctggttac atcctatgc actgaaagg gacagatat 
        tataaagtc gcaacggcg gtaagggat atatcacaa 
        gtaacgcct gaatgttca tttcgactt tcgggacta 
        caaaaacgg cgtactcgt ataactcaa atgccacca 
        attatggag ccggaagtt gaaatggaa ggtaatact 
        caaaagtcg aaaaggaaa actaagaaa ccggctaaa 
        aaacaatca agtaagaaa ctaaagaaa gatgagacc 
        ccaaaacgt caagaaacc agaactgtt tctgaatcg 
        gagacaaat ctaaccaga agtgattac attgttgga 
        gaaaatgta attacctcc gattatgtt agtgcagaa 
        aatgttgca caaaacact caaacacca tttgtaaga 
        ctagaagaa gtaacttca aggaacatt ggttctgaa 
        gaacttcat aacaccgtc ttttcttca ccaccgttc 
        caagcacaa gagagtatc aagaaaaat tctgttata 
        tctggaaga atatctaaa cggtctagt accaaaacc 
        agcgcgaaa aaaactagg gattccttg aaaaaggta 
        gtcgagact caacgcgac tcaagcggt aatttcatg 
        atctgtcca gcaactaat gagcttatt agatacagt 
        ttcaaaaat ctggtgtac aaaccgagc gctaatttg 
        cctggtgtc atgtacgct gtgacggaa gatctaaaa 
        ttgggttgg ttgaagata agcggagga atgtcaaag 
        ccattgttt aaaactaag aatcacact atgtcgttc 
        gtagttgta gaaggaaaa gcggaggtg gggatacag 
        gacaagtgt gatttgtat aaaaaacac gacatattt 
        attgttcca caaggagcg gaatattac ataaataac 
        aaaaataaa actaacccc ttgctattg gttttcttc 
        aaagtcgca tatgcggac aaaccgacg taataatga 
        tactcttgg cgacaaatt tagttagta gtgtttctt 
        acattatcg agttcattt gttatttag ttatacttt 
        tcattaaga atatgaata ttgtatctt taataaaaa 
        gtttacaga act
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY