Status
Please wait ...
Gene information
General information
TC001543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2441799..2445335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001543
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06750

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001543 RA
      MMGITGAFS SLGINERAR TPPKNHSTL SLPNLVASD 
      RPISVNFLP NCGCDDETE PSEQPVDFS KKYCETKQV 
      KNCTFTKVV KSYHNSFTM YAETDLDQP TDYSLRYAE 
      DDSDSDQCN KITKTETGE FVQDTIKTY CTEDTPYET 
      PFNFSTSTS MSDLRIDDK APLKAKDVT VINKDLAPP 
      KIKSEFSSG LMSPEKPVN YCEEGTPGY FSRVPSCGS 
      LNSIPKPEL KEEKKESRE PKAVKFEEV VNYAQETPL 
      MFSRTSSLA SLDSIEQHS IHDDRSSVV SDFSRLTSG 
      LISPSELPD SPSQTVPPS PKPHKNPKR KIVPKSSVF 
      EDAVKKFET ERTPSHFST ATSLSSLID EEIDAKTGE 
      VDGGVKEPE PEVLDNLSE SSEDEGILA ACINIGMQN 
      NRYSQAKAT PAPPSDIQA SYTFSKKSD IEKLDITGD 
      SVKTYCTED TPAILSHAG SNSDLSNLS LLGKSEGNK 
      ILEVDPGPS HDNVVTGLK TTEIPTTGS PPSRRSSFS 
      LSDDSDEAG EALLQECIL AGMPSKASI SGAPDVPKN 
      LPNPDLSRN QIIESESSD EDDKILSEC INIGITKNR 
      YTQPKIVND TIKSYCTED TPAILSHAG SCSDLSVLS 
      IQNGDEKEY FSDLSNFSS EEDEKLLEE CVKFGMAVI 
      AKNKSNYDK MGETSGSRQ KSKIENFSS SESDSDESE 
      QALLQQCIE AGMPKRPQS PDLTPAEKA LLEQCILEG 
      MKKCRNVPK KPFEDDSSE LTEAEQALL RQCILSGMP 
      KNKNLTQGG PSAVRESHE LSEAERALL QQCILAGMP 
      KNRRRKERV ERQEHTCCV HCPRKGARR KKYKVKESR 
      DVNIVYVTS QIVVKKEVD RGHEEWV
    CDS sequence
    • >TC001543 RA
      atgatgggc ataacgggc gccttctct agccttgga 
      attaatgag cgggcgcgt acgccccca aaaaatcac 
      agtacattg agcctccct aatttggtg gcttcagac 
      cgccccatt tcggtcaat tttctccca aattgtggc 
      tgtgacgac gaaacggaa ccaagcgaa caaccggtc 
      gattttagc aagaaatac tgtgagact aaacaagtc 
      aaaaactgc acctttact aaagttgtc aaaagttac 
      cacaatagt tttaccatg tacgcagag actgatttg 
      gaccaaccg actgactat tcgttacgt tacgctgag 
      gatgattct gattccgac cagtgcaat aaaatcaca 
      aagactgaa actggggag ttcgtgcaa gacactatt 
      aagacttat tgcacggaa gacacgcca tacgagacc 
      ccttttaat ttctccacg tccacgtca atgtctgac 
      ttacgaatc gacgataaa gcccccttg aaagccaaa 
      gacgtgacg gtgattaac aaagacttg gcccctcct 
      aaaatcaag tccgagttc agttccggg ctgatgtct 
      ccggaaaaa ccggtcaat tactgcgag gaaggcact 
      ccggggtac ttctcacgc gttcccagc tgtggctcc 
      ctaaattcg atcccgaaa ccggaacta aaggaagaa 
      aaaaaggaa tcaagggaa cccaaagct gtaaaattc 
      gaagaagtc gtaaattac gcccaagaa accccctta 
      atgttctca aggacgagc tctttggcg tccttggac 
      agcatcgaa caacactcg attcacgac gatcgcagt 
      tctgttgtt tcggatttc agtcgctta acgagcggt 
      ttgatttcg ccgagcgaa ttgcccgat tcgccctcg 
      cagacggtg cccccgagc cccaaaccg cacaaaaac 
      cccaaacga aagattgtg ccaaaatcg agcgttttc 
      gaagacgct gttaagaaa tttgaaacg gaacgaacg 
      ccttctcat ttttcgact gccactagt ttgagtagt 
      ttaattgac gaggaaatt gatgccaaa actggagag 
      gttgatggt ggtgttaag gaaccagag ccagaggtt 
      ttggataat ttgagtgaa agttcggaa gatgaggga 
      attttggcg gcttgtatc aacataggg atgcaaaat 
      aataggtat agtcaagct aaagcgaca ccagcgccc 
      ccaagtgac atacaagcg agttataca tttagtaaa 
      aagagtgat attgaaaaa ttggatatt acgggtgat 
      tcggttaag acgtactgt actgaagat actccggct 
      attttgagt catgctggg tcgaattct gatcttagc 
      aacttatct ttgctcgga aaatcggaa ggaaataaa 
      attttagaa gttgatcca ggacccagt catgataat 
      gtagtgaca gggctgaaa acaaccgaa attccaact 
      acgggaagt ccgccgtct cgccgttcg tctttttct 
      ctgagtgac gattcggac gaagcaggg gaggctctg 
      ctccaggaa tgtatttta gcaggaatg ccctcgaaa 
      gcgtcaatt tcgggggcc cctgacgtg ccaaaaaac 
      ttacctaat ccggacctg tcacgtaac caaatcatt 
      gaaagcgaa tcgtcggac gaagacgat aaaatttta 
      tcagagtgc ataaacatc ggaattaca aaaaatcgt 
      tatacacaa ccaaaaatt gtaaacgat acaattaag 
      agttactgc actgaagac actcctgcc attttaagt 
      cacgccggt tcttgctct gatctcagc gtcctctct 
      atccaaaac ggggacgaa aaagaatac ttttccgac 
      ctgagtaat ttttcgtca gaagaagac gaaaagtta 
      ttggaggag tgcgtcaag tttggaatg gccgtaatt 
      gccaaaaat aaatccaac tacgataaa atgggcgaa 
      acttccggg tcgagacaa aaaagcaaa attgaaaat 
      ttctcctct agtgaatca gattcggac gagtcggag 
      caagccctc ctccagcag tgcatcgag gccgggatg 
      cccaagcgc ccccagtcc cccgacttg acccccgct 
      gagaaagcc ctcctcgaa cagtgtatc cttgaaggg 
      atgaaaaag tgccgaaac gtcccgaaa aaacccttc 
      gaggacgac tcctcggaa ctgacggag gccgaacaa 
      gccctccta cggcagtgt atcctgtcc ggaatgccc 
      aaaaataag aacctgact caagggggc ccctcggcc 
      gtgagggaa tcccacgaa ttgagcgag gccgaaagg 
      gcactctta caacagtgt attttggcc ggaatgcca 
      aaaaacagg cggcggaaa gaacgggtg gagagacag 
      gagcacacg tgttgcgtg cactgtccc cgaaagggg 
      gccaggcgg aaaaaatac aaagtgaag gagagtcgc 
      gatgttaac attgtgtac gtcacgagt caaattgtg 
      gtgaagaag gaggtggat cgggggcac gaggagtgg 
      gtttga
    mRNA sequence
    • >TC001543 RA
      tcatttaag tgtcaaaag tttgtttat tgttgatgt 
      gtttttcgt gttgttttt cttaattgt tgtaacaag 
      tcgatatga tgggcataa cgggcgcct tctctagcc 
      ttggaatta atgagcggg cgcgtacgc ccccaaaaa 
      atcacagta cattgagcc tccctaatt tggtggctt 
      cagaccgcc ccatttcgg tcaattttc tcccaaatt 
      gtggctgtg acgacgaaa cggaaccaa gcgaacaac 
      cggtcgatt ttagcaaga aatactgtg agactaaac 
      aagtcaaaa actgcacct ttactaaag ttgtcaaaa 
      gttaccaca atagtttta ccatgtacg cagagactg 
      atttggacc aaccgactg actattcgt tacgttacg 
      ctgaggatg attctgatt ccgaccagt gcaataaaa 
      tcacaaaga ctgaaactg gggagttcg tgcaagaca 
      ctattaaga cttattgca cggaagaca cgccatacg 
      agacccctt ttaatttct ccacgtcca cgtcaatgt 
      ctgacttac gaatcgacg ataaagccc ccttgaaag 
      ccaaagacg tgacggtga ttaacaaag acttggccc 
      ctcctaaaa tcaagtccg agttcagtt ccgggctga 
      tgtctccgg aaaaaccgg tcaattact gcgaggaag 
      gcactccgg ggtacttct cacgcgttc ccagctgtg 
      gctccctaa attcgatcc cgaaaccgg aactaaagg 
      aagaaaaaa aggaatcaa gggaaccca aagctgtaa 
      aattcgaag aagtcgtaa attacgccc aagaaaccc 
      ccttaatgt tctcaagga cgagctctt tggcgtcct 
      tggacagca tcgaacaac actcgattc acgacgatc 
      gcagttctg ttgtttcgg atttcagtc gcttaacga 
      gcggtttga tttcgccga gcgaattgc ccgattcgc 
      cctcgcaga cggtgcccc cgagcccca aaccgcaca 
      aaaacccca aacgaaaga ttgtgccaa aatcgagcg 
      ttttcgaag acgctgtta agaaatttg aaacggaac 
      gaacgcctt ctcattttt cgactgcca ctagtttga 
      gtagtttaa ttgacgagg aaattgatg ccaaaactg 
      gagaggttg atggtggtg ttaaggaac cagagccag 
      aggttttgg ataatttga gtgaaagtt cggaagatg 
      agggaattt tggcggctt gtatcaaca tagggatgc 
      aaaataata ggtatagtc aagctaaag cgacaccag 
      cgcccccaa gtgacatac aagcgagtt atacattta 
      gtaaaaaga gtgatattg aaaaattgg atattacgg 
      gtgattcgg ttaagacgt actgtactg aagatactc 
      cggctattt tgagtcatg ctgggtcga attctgatc 
      ttagcaact tatctttgc tcggaaaat cggaaggaa 
      ataaaattt tagaagttg atccaggac ccagtcatg 
      ataatgtag tgacagggc tgaaaacaa ccgaaattc 
      caactacgg gaagtccgc cgtctcgcc gttcgtctt 
      tttctctga gtgacgatt cggacgaag caggggagg 
      ctctgctcc aggaatgta ttttagcag gaatgccct 
      cgaaagcgt caatttcgg gggcccctg acgtgccaa 
      aaaacttac ctaatccgg acctgtcac gtaaccaaa 
      tcattgaaa gcgaatcgt cggacgaag acgataaaa 
      ttttatcag agtgcataa acatcggaa ttacaaaaa 
      atcgttata cacaaccaa aaattgtaa acgatacaa 
      ttaagagtt actgcactg aagacactc ctgccattt 
      taagtcacg ccggttctt gctctgatc tcagcgtcc 
      tctctatcc aaaacgggg acgaaaaag aatactttt 
      ccgacctga gtaattttt cgtcagaag aagacgaaa 
      agttattgg aggagtgcg tcaagtttg gaatggccg 
      taattgcca aaaataaat ccaactacg ataaaatgg 
      gcgaaactt ccgggtcga gacaaaaaa gcaaaattg 
      aaaatttct cctctagtg aatcagatt cggacgagt 
      cggagcaag ccctcctcc agcagtgca tcgaggccg 
      ggatgccca agcgccccc agtcccccg acttgaccc 
      ccgctgaga aagccctcc tcgaacagt gtatccttg 
      aagggatga aaaagtgcc gaaacgtcc cgaaaaaac 
      ccttcgagg acgactcct cggaactga cggaggccg 
      aacaagccc tcctacggc agtgtatcc tgtccggaa 
      tgcccaaaa ataagaacc tgactcaag ggggcccct 
      cggccgtga gggaatccc acgaattga gcgaggccg 
      aaagggcac tcttacaac agtgtattt tggccggaa 
      tgccaaaaa acaggcggc ggaaagaac gggtggaga 
      gacaggagc acacgtgtt gcgtgcact gtccccgaa 
      agggggcca ggcggaaaa aatacaaag tgaaggaga 
      gtcgcgatg ttaacattg tgtacgtca cgagtcaaa 
      ttgtggtga agaaggagg tggatcggg ggcacgagg 
      agtgggttt gagggccaa atgttgtgt aattgtgat 
      aaataaatc aattttttt gca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY