Status
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Gene information
General information
TC031941
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001521,TC001522
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2953839..3021290
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031941
    iBeetle screen iB_06748

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031941 RA
      MAIRSACER RVRLAAFRR YTSHTPPGA ERARSSRGA 
      YEQPQQSSR GVLVCRRNH SSRCVGHMV QVLEVVKSK 
      NFDLPNSMN RPIQVKPAD SENRGDRKL FVGMLSKQQ 
      TEEDVRQLF APFGTIEEC TILRGPDGA SKGCAFVKY 
      SSHQEAQAA INSLHGSQT MPGASSSLV VKFADTEKE 
      RQLRRMQQM AGNMSLLNP FVFNQFGAY GAYAQHSLD 
      FQQQAALMA AATAQGTYI NPMAALATQ IPHATLNGM 
      ANSVVPPTS GAGSGQPVN GAIPSLPSP TMPTFNMAA 
      QTPNGQPGG TEAVYTNGI PQTYPAHAL HLSIPAQGL 
      PNGEAALQH AAYPGMQPY PGVAYPAVY GQFPQAIPQ 
      PMSAVAPAQ REGCSISGP EGCNLFIYH LPQEFGDAE 
      LMQMFLPFG NVISSKVFI DRATNQSKC FGFVSFDNP 
      ASAQAAIQA MNGFQIGMK RLKVQLKRP KDANRPY
    • >TC031941 RB
      MAIRSACER RVRLAAFRR YTSHTPPGA ERARSSRGA 
      YEQPQQSSR GVLVCRRNH SSRCVGHMV QVLEVVKSK 
      NFDLPNSMN RPIQVKPAD SENRGDRKL FVGMLSKQQ 
      TEEDVRQLF APFGTIEEC TILRGPDGA SKGCAFVKY 
      SSHQEAQAA INSLHGSQT MPGASSSLV VKFADTEKE 
      RQLRRMQQM AGNMSLLNP FVFNQFGAY GAYAQHSLD 
      FQQQAALMA AATAQGTYI NPMAALATQ IPHATLNGM 
      ANSVVPPTS DVGVGAGSG QPVNGAIPS LPSPTMPTF 
      NMAAQTPNG QPGGTEAVY TNGIPQTYP APQGLPNGE 
      AALQHAAYP GMQPYPGVA YPAVYGQFP QAIPQPMSA 
      VAPAQREGC SISGPEGCN LFIYHLPQE FGDAELMQM 
      FLPFGNVIS SKVFIDRAT NQSKCFGFV SFDNPASAQ 
      AAIQAMNGF QIGMKRLKV QLKRPKDAN RPY
    • >TC031941 RC
      MAIRSACER RVRLAAFRR YTSHTPPGA ERARSSRGA 
      YEQPQQSSR GVLVCRRNH SSRCVGHMV QVLEVVKSK 
      NFDLPNSMN RPIQVKPAD SENRGDRKL FVGMLSKQQ 
      TEEDVRQLF APFGTIEEC TILRGPDGA SKGCAFVKY 
      SSHQEAQAA INSLHGSQT MPGASSSLV VKFADTEKE 
      RQLRRMQQM AGNMSLLNP FVFNQFGAY GAYAQHSLD 
      FQQQAALMA AATAQGTYI NPMAALATQ IPHATLNGM 
      ANSVVPPTS DVGVGAGSG QPVNGAIPS LPSPTMPTF 
      NMAAQTPNG QPGGTEAVY TNGIPQTYP AHALHLSIP 
      AQGLPNGEA ALQHAAYPG MQPYPGVAY PAVYGQFPQ 
      AIPQPMSAV APAQREGCS ISGPEGCNL FIYHLPQEF 
      GDAELMQMF LPFGNVISS KVFIDRATN QSKCFGFVS 
      FDNPASAQA AIQAMNGFQ IGMKRLKVQ LKRPKDANR 
      PY
    CDS sequence
    • >TC031941 RA
      atggccata aggagtgcg tgcgagagg cgcgtgcga 
      ttggctgcg tttcgtcga tacacctcg cacactcct 
      ccgggcgca gaacgcgcc cggagttca aggggtgcg 
      tacgaacaa ccccaacaa tccagtcgc ggcgtcttg 
      gtgtgtcgt cgaaatcac agttcacgt tgcgtcggc 
      catatggtg caagtactg gaggtggtc aagtcgaaa 
      aacttcgac ttgcccaat tctatgaac cgccccatc 
      caagtgaaa ccagcggac agtgaaaac cgcggagat 
      cgaaagctg ttcgtgggt atgctcagt aagcaacaa 
      accgaagaa gatgtcaga caattattc gcgcctttt 
      ggaactata gaagagtgc acgattctt cggggaccc 
      gacggagct agcaaaggt tgcgctttc gtgaaatac 
      agttcgcat caggaagcg caggcggcc attaatagc 
      ttacacggg agccaaact atgccggga gcttcttcc 
      agcttagta gtgaaattt gccgatact gaaaaggaa 
      cgacaactc agacgcatg cagcagatg gccggaaac 
      atgagtctt ctcaacccc ttcgtgttc aaccagttc 
      ggagcttac ggagcttac gcacagcat tctttggac 
      tttcagcaa caggcggct ttgatggca gccgcgaca 
      gcacaagga acttacata aacccgatg gcagccctt 
      gccacgcaa atcccgcac gcgaccctc aacggaatg 
      gcgaattcg gtcgttccg ccgacttca ggtgctggg 
      tccgggcag ccggtgaac ggcgccatc cccagcctg 
      ccgtcgccc accatgccc accttcaac atggcggcg 
      cagaccccc aacggccag cccgggggc acagaggcc 
      gtctacacg aacggcatc ccccagacg tatccagct 
      catgcgcta catctgtcc attccagcg caaggattg 
      cctaatgga gaagcggcg ctacagcac gcagcgtat 
      ccagggatg caaccatat ccaggagtc gcttacccc 
      gcggtttat ggccaattc ccacaggcc attccccaa 
      cctatgtca gcagttgct cctgcacag agggaaggc 
      tgctctatt tccggacct gaaggctgc aatttgttt 
      atctaccat ttacctcaa gaattcggt gacgctgaa 
      ctgatgcaa atgttcctg cccttcgga aatgtcatc 
      agctcaaaa gtgttcatt gaccgtgcc actaaccag 
      agcaaatgt ttcgggttc gtgtccttc gacaacccg 
      gcaagcgct caagcagcc atccaagct atgaacggc 
      ttccagatc ggcatgaag cgactcaaa gtgcagctg 
      aagaggccc aaggacgcc aacaggccg tactaa
    • >TC031941 RB
      atggccata aggagtgcg tgcgagagg cgcgtgcga 
      ttggctgcg tttcgtcga tacacctcg cacactcct 
      ccgggcgca gaacgcgcc cggagttca aggggtgcg 
      tacgaacaa ccccaacaa tccagtcgc ggcgtcttg 
      gtgtgtcgt cgaaatcac agttcacgt tgcgtcggc 
      catatggtg caagtactg gaggtggtc aagtcgaaa 
      aacttcgac ttgcccaat tctatgaac cgccccatc 
      caagtgaaa ccagcggac agtgaaaac cgcggagat 
      cgaaagctg ttcgtgggt atgctcagt aagcaacaa 
      accgaagaa gatgtcaga caattattc gcgcctttt 
      ggaactata gaagagtgc acgattctt cggggaccc 
      gacggagct agcaaaggt tgcgctttc gtgaaatac 
      agttcgcat caggaagcg caggcggcc attaatagc 
      ttacacggg agccaaact atgccggga gcttcttcc 
      agcttagta gtgaaattt gccgatact gaaaaggaa 
      cgacaactc agacgcatg cagcagatg gccggaaac 
      atgagtctt ctcaacccc ttcgtgttc aaccagttc 
      ggagcttac ggagcttac gcacagcat tctttggac 
      tttcagcaa caggcggct ttgatggca gccgcgaca 
      gcacaagga acttacata aacccgatg gcagccctt 
      gccacgcaa atcccgcac gcgaccctc aacggaatg 
      gcgaattcg gtcgttccg ccgacttca gatgttggc 
      gtaggtgct gggtccggg cagccggtg aacggcgcc 
      atccccagc ctgccgtcg cccaccatg cccaccttc 
      aacatggcg gcgcagacc cccaacggc cagcccggg 
      ggcacagag gccgtctac acgaacggc atcccccag 
      acgtatcca gctccgcaa ggattgcct aatggagaa 
      gcggcgcta cagcacgca gcgtatcca gggatgcaa 
      ccatatcca ggagtcgct taccccgcg gtttatggc 
      caattccca caggccatt ccccaacct atgtcagca 
      gttgctcct gcacagagg gaaggctgc tctatttcc 
      ggacctgaa ggctgcaat ttgtttatc taccattta 
      cctcaagaa ttcggtgac gctgaactg atgcaaatg 
      ttcctgccc ttcggaaat gtcatcagc tcaaaagtg 
      ttcattgac cgtgccact aaccagagc aaatgtttc 
      gggttcgtg tccttcgac aacccggca agcgctcaa 
      gcagccatc caagctatg aacggcttc cagatcggc 
      atgaagcga ctcaaagtg cagctgaag aggcccaag 
      gacgccaac aggccgtac taa
    • >TC031941 RC
      atggccata aggagtgcg tgcgagagg cgcgtgcga 
      ttggctgcg tttcgtcga tacacctcg cacactcct 
      ccgggcgca gaacgcgcc cggagttca aggggtgcg 
      tacgaacaa ccccaacaa tccagtcgc ggcgtcttg 
      gtgtgtcgt cgaaatcac agttcacgt tgcgtcggc 
      catatggtg caagtactg gaggtggtc aagtcgaaa 
      aacttcgac ttgcccaat tctatgaac cgccccatc 
      caagtgaaa ccagcggac agtgaaaac cgcggagat 
      cgaaagctg ttcgtgggt atgctcagt aagcaacaa 
      accgaagaa gatgtcaga caattattc gcgcctttt 
      ggaactata gaagagtgc acgattctt cggggaccc 
      gacggagct agcaaaggt tgcgctttc gtgaaatac 
      agttcgcat caggaagcg caggcggcc attaatagc 
      ttacacggg agccaaact atgccggga gcttcttcc 
      agcttagta gtgaaattt gccgatact gaaaaggaa 
      cgacaactc agacgcatg cagcagatg gccggaaac 
      atgagtctt ctcaacccc ttcgtgttc aaccagttc 
      ggagcttac ggagcttac gcacagcat tctttggac 
      tttcagcaa caggcggct ttgatggca gccgcgaca 
      gcacaagga acttacata aacccgatg gcagccctt 
      gccacgcaa atcccgcac gcgaccctc aacggaatg 
      gcgaattcg gtcgttccg ccgacttca gatgttggc 
      gtaggtgct gggtccggg cagccggtg aacggcgcc 
      atccccagc ctgccgtcg cccaccatg cccaccttc 
      aacatggcg gcgcagacc cccaacggc cagcccggg 
      ggcacagag gccgtctac acgaacggc atcccccag 
      acgtatcca gctcatgcg ctacatctg tccattcca 
      gcgcaagga ttgcctaat ggagaagcg gcgctacag 
      cacgcagcg tatccaggg atgcaacca tatccagga 
      gtcgcttac cccgcggtt tatggccaa ttcccacag 
      gccattccc caacctatg tcagcagtt gctcctgca 
      cagagggaa ggctgctct atttccgga cctgaaggc 
      tgcaatttg tttatctac catttacct caagaattc 
      ggtgacgct gaactgatg caaatgttc ctgcccttc 
      ggaaatgtc atcagctca aaagtgttc attgaccgt 
      gccactaac cagagcaaa tgtttcggg ttcgtgtcc 
      ttcgacaac ccggcaagc gctcaagca gccatccaa 
      gctatgaac ggcttccag atcggcatg aagcgactc 
      aaagtgcag ctgaagagg cccaaggac gccaacagg 
      ccgtactaa
    mRNA sequence
    • >TC031941 RA
      tgtggaagc gtaatggcc ataaggagt gcgtgcgag 
      aggcgcgtg cgattggct gcgtttcgt cgatacacc 
      tcgcacact cctccgggc gcagaacgc gcccggagt 
      tcaaggggt gcgtacgaa caaccccaa caatccagt 
      cgcggcgtc ttggtgtgt cgtcgaaat cacagttca 
      cgttgcgtc ggccatatg gtgcaagta ctggaggtg 
      gtcaagtcg aaaaacttc gacttgccc aattctatg 
      aaccgcccc atccaagtg aaaccagcg gacagtgaa 
      aaccgcgga gatcgaaag ctgttcgtg ggtatgctc 
      agtaagcaa caaaccgaa gaagatgtc agacaatta 
      ttcgcgcct tttggaact atagaagag tgcacgatt 
      cttcgggga cccgacgga gctagcaaa ggttgcgct 
      ttcgtgaaa tacagttcg catcaggaa gcgcaggcg 
      gccattaat agcttacac gggagccaa actatgccg 
      ggagcttct tccagctta gtagtgaaa tttgccgat 
      actgaaaag gaacgacaa ctcagacgc atgcagcag 
      atggccgga aacatgagt cttctcaac cccttcgtg 
      ttcaaccag ttcggagct tacggagct tacgcacag 
      cattctttg gactttcag caacaggcg gctttgatg 
      gcagccgcg acagcacaa ggaacttac ataaacccg 
      atggcagcc cttgccacg caaatcccg cacgcgacc 
      ctcaacgga atggcgaat tcggtcgtt ccgccgact 
      tcaggtgct gggtccggg cagccggtg aacggcgcc 
      atccccagc ctgccgtcg cccaccatg cccaccttc 
      aacatggcg gcgcagacc cccaacggc cagcccggg 
      ggcacagag gccgtctac acgaacggc atcccccag 
      acgtatcca gctcatgcg ctacatctg tccattcca 
      gcgcaagga ttgcctaat ggagaagcg gcgctacag 
      cacgcagcg tatccaggg atgcaacca tatccagga 
      gtcgcttac cccgcggtt tatggccaa ttcccacag 
      gccattccc caacctatg tcagcagtt gctcctgca 
      cagagggaa ggctgctct atttccgga cctgaaggc 
      tgcaatttg tttatctac catttacct caagaattc 
      ggtgacgct gaactgatg caaatgttc ctgcccttc 
      ggaaatgtc atcagctca aaagtgttc attgaccgt 
      gccactaac cagagcaaa tgtttcggg ttcgtgtcc 
      ttcgacaac ccggcaagc gctcaagca gccatccaa 
      gctatgaac ggcttccag atcggcatg aagcgactc 
      aaagtgcag ctgaagagg cccaaggac gccaacagg 
      ccgtactaa ccttggccc tcagatctc ttggcgctc 
      gtagcgccc tcagccact agtatacta gacgacact 
      ttatataag cacatagaa tattgtaac ttatatata 
      taaaaaaaa ataaaacta aaagagcat gcctggcac 
      acactccga gcacacgct gcagtccgg ctgcgcccc 
      cgcccctca tttcgagaa attccaaat gggctgtga 
      ttaaataac aaatcagtg tttgattgt acaggccgt 
      tttgtcttc tcgttcgtg aaatgtatt actattttg 
      agacgtcgt ctactctta tttgttgta cttatgtgt 
      gttaatctg taagaaaaa gggccgggg gcggcagcc 
      cctgcccag ttgccaaat ttcggtcag gcatgcctt 
      ctgtgatgc tactgctag tcacagctc ggctcggag 
      tcgctctgg gggcacacg gtaccatgt cccccttct 
      gactgcaag gagccacga catatttat atatttaac 
      aatactatt actaaaaca aaaggaaaa tagtgtaat 
      atttctact aaagttaag taacataat ccttacaaa 
      gtaccaact atatattaa tctgtaaat tactagggg 
      gtagcgata ggatagcca aaagggtgg tcgtgatac 
      catgaaggt atagtcatt atcgatttt agaacaata 
      cgttcgcaa tttatgaat tttatctaa tgtataagt 
      ttatatatc aaaatgcaa attgtgcgt tttcgccaa 
      atagttgtt gaagagtta gttgacgct ttgcggggc 
      ccgcgggcc ccaaggcag gccgggaga acagagtgg 
      aggggcact cggctgagc cactctagt caaattatc 
      cagccaaaa tcggctgcg gattctagt taatatgat 
      tagaattaa tttagtcct aagacatat atttagtaa 
      aaaaaaaca tatatacta tttattgaa tctaacgta 
      tttagatat acaaggaag attttttta tttgttcat 
      ttcggctga gataatatc gtgcaatat taacagaac 
      gactgatac acattattt tttctaccg attcggttt 
      tcttcttct tctctgtgg ttttgaaac gaaacagct 
      ttaagtttt gtcgatttt ttcggtttg gtttttaag 
      tagttgtag tgcattcca cgcttgcta atgacattc 
      cttcagcgc tggaagtaa atatactta agtactaac 
      cacgggggg gtggcagct cccgattat aacgagaca 
      ccacaccac caaacagac acactcgaa ctcaaacga 
      aaagttcaa acgtgaaac aaacatcaa gaaaaaaat 
      tcactcaaa aaacaatat tttagattt actcatttc 
      ttaatcaaa attttacaa tccaagagt aagggaagg 
      agagagaca taaggttga gttgagttc aaacgaatg 
      tatgttcaa attaagaaa aaattaaag ttaattttt 
      aaataaaat aatttcttt aacacggat cttgcccca 
      aaatcaaac cagtttaag tcagttaag gttcaagta 
      gagaaaatt tattctttg aaacacatt taatttgtg 
      gttttttga tttacatat aaaactccg tgaaaaaat 
      taatttttt taatataga atttttata aat
    • >TC031941 RB
      tgtggaagc gtaatggcc ataaggagt gcgtgcgag 
      aggcgcgtg cgattggct gcgtttcgt cgatacacc 
      tcgcacact cctccgggc gcagaacgc gcccggagt 
      tcaaggggt gcgtacgaa caaccccaa caatccagt 
      cgcggcgtc ttggtgtgt cgtcgaaat cacagttca 
      cgttgcgtc ggccatatg gtgcaagta ctggaggtg 
      gtcaagtcg aaaaacttc gacttgccc aattctatg 
      aaccgcccc atccaagtg aaaccagcg gacagtgaa 
      aaccgcgga gatcgaaag ctgttcgtg ggtatgctc 
      agtaagcaa caaaccgaa gaagatgtc agacaatta 
      ttcgcgcct tttggaact atagaagag tgcacgatt 
      cttcgggga cccgacgga gctagcaaa ggttgcgct 
      ttcgtgaaa tacagttcg catcaggaa gcgcaggcg 
      gccattaat agcttacac gggagccaa actatgccg 
      ggagcttct tccagctta gtagtgaaa tttgccgat 
      actgaaaag gaacgacaa ctcagacgc atgcagcag 
      atggccgga aacatgagt cttctcaac cccttcgtg 
      ttcaaccag ttcggagct tacggagct tacgcacag 
      cattctttg gactttcag caacaggcg gctttgatg 
      gcagccgcg acagcacaa ggaacttac ataaacccg 
      atggcagcc cttgccacg caaatcccg cacgcgacc 
      ctcaacgga atggcgaat tcggtcgtt ccgccgact 
      tcagatgtt ggcgtaggt gctgggtcc gggcagccg 
      gtgaacggc gccatcccc agcctgccg tcgcccacc 
      atgcccacc ttcaacatg gcggcgcag acccccaac 
      ggccagccc gggggcaca gaggccgtc tacacgaac 
      ggcatcccc cagacgtat ccagctccg caaggattg 
      cctaatgga gaagcggcg ctacagcac gcagcgtat 
      ccagggatg caaccatat ccaggagtc gcttacccc 
      gcggtttat ggccaattc ccacaggcc attccccaa 
      cctatgtca gcagttgct cctgcacag agggaaggc 
      tgctctatt tccggacct gaaggctgc aatttgttt 
      atctaccat ttacctcaa gaattcggt gacgctgaa 
      ctgatgcaa atgttcctg cccttcgga aatgtcatc 
      agctcaaaa gtgttcatt gaccgtgcc actaaccag 
      agcaaatgt ttcgggttc gtgtccttc gacaacccg 
      gcaagcgct caagcagcc atccaagct atgaacggc 
      ttccagatc ggcatgaag cgactcaaa gtgcagctg 
      aagaggccc aaggacgcc aacaggccg tactaacct 
      tggccctca gatctcttg gcgctcgta gcgccctca 
      gccactagt atactagac gacacttta tataagcac 
      atagaatat tgtaactta tatatataa aaaaaaata 
      aaactaaaa gagcatgcc tggcacaca ctccgagca 
      cacgctgca gtccggctg cgcccccgc ccctcattt 
      cgagaaatt ccaaatggg ctgtgatta aataacaaa 
      tcagtgttt gattgtaca ggccgtttt gtcttctcg 
      ttcgtgaaa tgtattact attttgaga cgtcgtcta 
      ctcttattt gttgtactt atgtgtgtt aatctgtaa 
      gaaaaaggg ccgggggcg gcagcccct gcccagttg 
      ccaaatttc ggtcaggca tgccttctg tgatgctac 
      tgctagtca cagctcggc tcggagtcg ctctggggg 
      cacacggta ccatgtccc ccttctgac tgcaaggag 
      ccacgacat atttatata tttaacaat actattact 
      aaaacaaaa ggaaaatag tgtaatatt tctactaaa 
      gttaagtaa cataatcct tacaaagta ccaactata 
      tattaatct gtaaattac tagggggta gcgatagga 
      tagccaaaa gggtggtcg tgataccat gaaggtata 
      gtcattatc gattttaga acaatacgt tcgcaattt 
      atgaatttt atctaatgt ataagttta tatatcaaa 
      atgcaaatt gtgcgtttt cgccaaata gttgttgaa 
      gagttagtt gacgctttg cggggcccg cgggcccca 
      aggcaggcc gggagaaca gagtggagg ggcactcgg 
      ctgagccac tctagtcaa attatccag ccaaaatcg 
      gctgcggat tctagttaa tatgattag aattaattt 
      agtcctaag acatatatt tagtaaaaa aaaacatat 
      atactattt attgaatct aacgtattt agatataca 
      aggaagatt tttttattt gttcatttc ggctgagat 
      aatatcgtg caatattaa cagaacgac tgatacaca 
      ttatttttt ctaccgatt cggttttct tcttcttct 
      ctgtggttt tgaaacgaa acagcttta agttttgtc 
      gattttttc ggtttggtt tttaagtag ttgtagtgc 
      attccacgc ttgctaatg acattcctt cagcgctgg 
      aagtaaata tacttaagt actaaccac gggggggtg 
      gcagctccc gattataac gagacacca caccaccaa 
      acagacaca ctcgaactc aaacgaaaa gttcaaacg 
      tgaaacaaa catcaagaa aaaaattca ctcaaaaaa 
      caatatttt agatttact catttctta atcaaaatt 
      ttacaatcc aagagtaag ggaaggaga gagacataa 
      ggttgagtt gagttcaaa cgaatgtat gttcaaatt 
      aagaaaaaa ttaaagtta atttttaaa taaaataat 
      ttctttaac acggatctt gccccaaaa tcaaaccag 
      tttaagtca gttaaggtt caagtagag aaaatttat 
      tctttgaaa cacatttaa tttgtggtt ttttgattt 
      acatataaa actccgtga aaaaattaa tttttttaa 
      tatagaatt tttataaat
    • >TC031941 RC
      tgtggaagc gtaatggcc ataaggagt gcgtgcgag 
      aggcgcgtg cgattggct gcgtttcgt cgatacacc 
      tcgcacact cctccgggc gcagaacgc gcccggagt 
      tcaaggggt gcgtacgaa caaccccaa caatccagt 
      cgcggcgtc ttggtgtgt cgtcgaaat cacagttca 
      cgttgcgtc ggccatatg gtgcaagta ctggaggtg 
      gtcaagtcg aaaaacttc gacttgccc aattctatg 
      aaccgcccc atccaagtg aaaccagcg gacagtgaa 
      aaccgcgga gatcgaaag ctgttcgtg ggtatgctc 
      agtaagcaa caaaccgaa gaagatgtc agacaatta 
      ttcgcgcct tttggaact atagaagag tgcacgatt 
      cttcgggga cccgacgga gctagcaaa ggttgcgct 
      ttcgtgaaa tacagttcg catcaggaa gcgcaggcg 
      gccattaat agcttacac gggagccaa actatgccg 
      ggagcttct tccagctta gtagtgaaa tttgccgat 
      actgaaaag gaacgacaa ctcagacgc atgcagcag 
      atggccgga aacatgagt cttctcaac cccttcgtg 
      ttcaaccag ttcggagct tacggagct tacgcacag 
      cattctttg gactttcag caacaggcg gctttgatg 
      gcagccgcg acagcacaa ggaacttac ataaacccg 
      atggcagcc cttgccacg caaatcccg cacgcgacc 
      ctcaacgga atggcgaat tcggtcgtt ccgccgact 
      tcagatgtt ggcgtaggt gctgggtcc gggcagccg 
      gtgaacggc gccatcccc agcctgccg tcgcccacc 
      atgcccacc ttcaacatg gcggcgcag acccccaac 
      ggccagccc gggggcaca gaggccgtc tacacgaac 
      ggcatcccc cagacgtat ccagctcat gcgctacat 
      ctgtccatt ccagcgcaa ggattgcct aatggagaa 
      gcggcgcta cagcacgca gcgtatcca gggatgcaa 
      ccatatcca ggagtcgct taccccgcg gtttatggc 
      caattccca caggccatt ccccaacct atgtcagca 
      gttgctcct gcacagagg gaaggctgc tctatttcc 
      ggacctgaa ggctgcaat ttgtttatc taccattta 
      cctcaagaa ttcggtgac gctgaactg atgcaaatg 
      ttcctgccc ttcggaaat gtcatcagc tcaaaagtg 
      ttcattgac cgtgccact aaccagagc aaatgtttc 
      gggttcgtg tccttcgac aacccggca agcgctcaa 
      gcagccatc caagctatg aacggcttc cagatcggc 
      atgaagcga ctcaaagtg cagctgaag aggcccaag 
      gacgccaac aggccgtac taaccttgg ccctcagat 
      ctcttggcg ctcgtagcg ccctcagcc actagtata 
      ctagacgac actttatat aagcacata gaatattgt 
      aacttatat atataaaaa aaaataaaa ctaaaagag 
      catgcctgg cacacactc cgagcacac gctgcagtc 
      cggctgcgc ccccgcccc tcatttcga gaaattcca 
      aatgggctg tgattaaat aacaaatca gtgtttgat 
      tgtacaggc cgttttgtc ttctcgttc gtgaaatgt 
      attactatt ttgagacgt cgtctactc ttatttgtt 
      gtacttatg tgtgttaat ctgtaagaa aaagggccg 
      ggggcggca gcccctgcc cagttgcca aatttcggt 
      caggcatgc cttctgtga tgctactgc tagtcacag 
      ctcggctcg gagtcgctc tgggggcac acggtacca 
      tgtccccct tctgactgc aaggagcca cgacatatt 
      tatatattt aacaatact attactaaa acaaaagga 
      aaatagtgt aatatttct actaaagtt aagtaacat 
      aatccttac aaagtacca actatatat taatctgta 
      aattactag ggggtagcg ataggatag ccaaaaggg 
      tggtcgtga taccatgaa ggtatagtc attatcgat 
      tttagaaca atacgttcg caatttatg aattttatc 
      taatgtata agtttatat atcaaaatg caaattgtg 
      cgttttcgc caaatagtt gttgaagag ttagttgac 
      gctttgcgg ggcccgcgg gccccaagg caggccggg 
      agaacagag tggaggggc actcggctg agccactct 
      agtcaaatt atccagcca aaatcggct gcggattct 
      agttaatat gattagaat taatttagt cctaagaca 
      tatatttag taaaaaaaa acatatata ctatttatt 
      gaatctaac gtatttaga tatacaagg aagattttt 
      ttatttgtt catttcggc tgagataat atcgtgcaa 
      tattaacag aacgactga tacacatta ttttttcta 
      ccgattcgg ttttcttct tcttctctg tggttttga 
      aacgaaaca gctttaagt tttgtcgat tttttcggt 
      ttggttttt aagtagttg tagtgcatt ccacgcttg 
      ctaatgaca ttccttcag cgctggaag taaatatac 
      ttaagtact aaccacggg ggggtggca gctcccgat 
      tataacgag acaccacac caccaaaca gacacactc 
      gaactcaaa cgaaaagtt caaacgtga aacaaacat 
      caagaaaaa aattcactc aaaaaacaa tattttaga 
      tttactcat ttcttaatc aaaatttta caatccaag 
      agtaaggga aggagagag acataaggt tgagttgag 
      ttcaaacga atgtatgtt caaattaag aaaaaatta 
      aagttaatt tttaaataa aataatttc tttaacacg 
      gatcttgcc ccaaaatca aaccagttt aagtcagtt 
      aaggttcaa gtagagaaa atttattct ttgaaacac 
      atttaattt gtggttttt tgatttaca tataaaact 
      ccgtgaaaa aattaattt ttttaatat agaattttt 
      ataaat
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