Status
Please wait ...
Gene information
General information
TC015964 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450622.1: 5..3775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015964
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06746

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015964 RA
      MDVFELSND DKFVDLHGR EIPAVRNKQ EDIALSLAE 
      LKPAGVAIY QKSQYEKLS KIDVPFDSD FVDYMLESA 
      KNYMLNNKV SFSQDQIMR SIENCKSIL IDNKNPAPH 
      KQPSFLQNR SVAKGGDDE ILIQVTNKP SQFQPPSAK 
      HKIPNFKNI NPKLHVSPK TSPKSIKVT SQVENGTRK 
      PKVDTDKAQ DGTTKLKNG SDQTQGAKS LKRFTYNIV 
      QEERTVYVT KIDKRNCIW VRDKANDEI FELIHDKVN 
      EEAKHQKVP SKPWKTEKV YMAQDKGCW FRCKIARMF 
      PLTVFFLDY GNVAEVDEN REISEDLAK IPCMATRIT 
      FKEAEYLPI LEDEIRIKN FYYNRDGPY FVNILSSAK 
      KAPIEQTKH SQRANEIKQ THPTKTIND VFFKKPPLL 
      CEISDGQTV VIVQVVEEM FYLKTKECF AKLKVVKLM 
      IEEYSKNAK SVENVKVNQ VILHYNSKT NKLNRAIVL 
      KVNNNNVEI ETFDYPGRF TTDLENLKS ISEQLSKEP 
      VTCLPGGRL TGFENKQLG EDCKEIIAK HIQKRTKFR 
      VVLGFDDEL DLFDGNLLL SEQLQTVLE GPRKIYVQD 
      VTPYRPAIG VNSFVCGYY NSSDDFAIV PMKELQEHI 
      DCILRCDVE DNVPYVVDV KEVCLCYFV DQWYRCVVT 
      RIFDKDCEV RLLDFGNVT VIRKENLRR PDDDIMKVP 
      ILALPCKLT GLPHQNVEA ALKKIILKG EIFNRLM
    CDS sequence
    • >TC015964 RA
      atggacgta ttcgaatta agcaatgat gacaaattc 
      gtcgactta cacggccga gaaatacct gcagtaaga 
      aataagcag gaagacatt gcactgtct ctggcagaa 
      ttaaaacca gcaggtgtg gctatttat caaaagagc 
      cagtatgaa aaactttcc aaaattgat gtaccattt 
      gatagcgat tttgttgac tacatgctt gaatcagca 
      aaaaattac atgctgaac aataaagta tcattctcc 
      caagatcag attatgaga agcattgag aattgcaag 
      agcatttta atagacaac aaaaatcct gctcctcac 
      aagcagccc tctttctta caaaacaga tctgtagct 
      aaaggaggc gatgatgaa attctgatt caagtcact 
      aacaaaccc agccagttc cagcctcca agcgctaaa 
      cataaaatt ccaaacttt aaaaacatc aatcctaaa 
      ttacacgta tctccaaaa acaagccct aaatcaata 
      aaagtaacc agtcaagtt gaaaatggt acaaggaag 
      ccgaaagtt gatactgat aaagcgcaa gatgggacc 
      acgaaacta aaaaatggt tctgaccaa acacaaggt 
      gccaagtct cttaaaagg tttacttat aacatagtt 
      caagaagaa cgtacggtt tatgtgaca aaaattgat 
      aagcggaac tgcatttgg gtgcgagat aaggccaat 
      gacgaaatc tttgagtta attcacgat aaagtgaac 
      gaagaagcg aagcaccaa aaggtgccc tcaaagcct 
      tggaaaact gaaaaagta tacatggct caggataaa 
      ggttgctgg tttaggtgt aaaatcgcc aggatgttt 
      ccgctgact gttttcttc ctggattac ggaaacgtg 
      gcagaagtg gacgaaaac agggagatt tcagaagac 
      ttggcaaaa attccctgc atggcaaca cgcattaca 
      tttaaggaa gcggagtat ttgccaatt ctggaagac 
      gaaatccga ataaagaat ttctactac aacagggat 
      ggaccttac tttgtcaac attttaagt tcggccaaa 
      aaagcacca attgaacaa actaaacac tcccagcgg 
      gcaaacgag ataaaacaa acacacccc acaaagaca 
      ataaatgat gtcttcttc aagaaacct ccacttttg 
      tgcgaaatc agtgacggc caaactgtg gtaattgtg 
      caagtggtt gaagaaatg ttttattta aaaactaaa 
      gaatgtttt gctaagtta aaagttgtt aaattaatg 
      atcgaagag tactctaaa aatgctaaa agcgttgaa 
      aatgttaaa gtgaatcaa gttattttg cattataac 
      agcaaaacc aataaactt aaccgagct atcgtactg 
      aaagttaat aacaataat gttgaaatt gaaactttc 
      gattatcca ggacgattt actacagat ttggaaaat 
      ctaaaatcg atcagtgaa caactgagt aaagaacca 
      gtcacttgt ctcccaggg gggcgcttg accggattt 
      gaaaacaaa caactcggg gaagattgc aaggaaatt 
      attgcaaaa catatccaa aagcggaca aaattccgg 
      gtggtgttg ggtttcgac gacgaactt gatttattt 
      gacggtaat ctgcttctc tcagagcaa ttgcaaaca 
      gtactagaa ggtcccaga aaaatttat gttcaagac 
      gtaacgcct tacagaccc gcaatagga gtcaactca 
      tttgtgtgc ggttattac aatagcagc gacgatttt 
      gcgattgta ccaatgaag gaattgcaa gaacacatt 
      gactgtatt ttaagatgt gatgtcgaa gataatgta 
      ccatacgtt gttgatgtg aaggaagtc tgtttgtgt 
      tattttgtc gatcagtgg tatagatgt gtagttacg 
      aggattttt gataaggat tgtgaagtt aggttactg 
      gattttggt aatgttaca gttatcaga aaagaaaat 
      ttaaggaga cctgacgat gacatcatg aaagtgcca 
      attttggct ttaccgtgt aaactcact ggtctgcca 
      catcaaaat gttgaagca gcgttaaaa aagataatt 
      cttaaggga gagattttc aaccggttg atgtaa
    mRNA sequence
    • >TC015964 RA
      tacgaaaat aaaatatga gaacaaatg aagacaata 
      aacttgcca gttactaat tacaactcc aacacatac 
      taaaaagaa cttgggttt ttgtaaact ggtgtcaaa 
      taatcttaa gtctgcttt atttagatt tacattgga 
      aaggcacgt gggggaaag aaccaagag ttggttatg 
      tgtcaatgg ttacataac ctcaaaaac ttcaacgtc 
      gtctgttta ccatttaaa agagataaa aataaatgc 
      cgtttttta ctcgtttat ttataaaaa atatttaaa 
      tttttcaaa ctggtttta aataatcat ctttttaag 
      taatatgga cgtattcga attaagcaa tgatgacaa 
      attcgtcga cttacacgg ccgagaaat acctgcagt 
      aagaaataa gcaggaaga cattgcact gtctctggc 
      agaattaaa accagcagg tgtggctat ttatcaaaa 
      gagccagta tgaaaaact ttccaaaat tgatgtacc 
      atttgatag cgattttgt tgactacat gcttgaatc 
      agcaaaaaa ttacatgct gaacaataa agtatcatt 
      ctcccaaga tcagattat gagaagcat tgagaattg 
      caagagcat tttaataga caacaaaaa tcctgctcc 
      tcacaagca gccctcttt cttacaaaa cagatctgt 
      agctaaagg aggcgatga tgaaattct gattcaagt 
      cactaacaa acccagcca gttccagcc tccaagcgc 
      taaacataa aattccaaa ctttaaaaa catcaatcc 
      taaattaca cgtatctcc aaaaacaag ccctaaatc 
      aataaaagt aaccagtca agttgaaaa tggtacaag 
      gaagccgaa agttgatac tgataaagc gcaagatgg 
      gaccacgaa actaaaaaa tggttctga ccaaacaca 
      aggtgccaa gtctcttaa aaggtttac ttataacat 
      agttcaaga agaacgtac ggtttatgt gacaaaaat 
      tgataagcg gaactgcat ttgggtgcg agataaggc 
      caatgacga aatctttga gttaattca cgataaagt 
      gaacgaaga agcgaagca ccaaaaggt gccctcaaa 
      gccttggaa aactgaaaa agtatacat ggctcagga 
      taaaggttg ctggtttag gtgtaaaat cgccaggat 
      gtttccgct gactgtttt cttcctgga ttacggaaa 
      cgtggcaga agtggacga aaacaggga gatttcaga 
      agacttggc aaaaattcc ctgcatggc aacacgcat 
      tacatttaa ggaagcgga gtatttgcc aattctgga 
      agacgaaat ccgaataaa gaatttcta ctacaacag 
      ggatggacc ttactttgt caacatttt aagttcggc 
      caaaaaagc accaattga acaaactaa acactccca 
      gcgggcaaa cgagataaa acaaacaca ccccacaaa 
      gacaataaa tgatgtctt cttcaagaa acctccact 
      tttgtgcga aatcagtga cggccaaac tgtggtaat 
      tgtgcaagt ggttgaaga aatgtttta tttaaaaac 
      taaagaatg ttttgctaa gttaaaagt tgttaaatt 
      aatgatcga agagtactc taaaaatgc taaaagcgt 
      tgaaaatgt taaagtgaa tcaagttat tttgcatta 
      taacagcaa aaccaataa acttaaccg agctatcgt 
      actgaaagt taataacaa taatgttga aattgaaac 
      tttcgatta tccaggacg atttactac agatttgga 
      aaatctaaa atcgatcag tgaacaact gagtaaaga 
      accagtcac ttgtctccc aggggggcg cttgaccgg 
      atttgaaaa caaacaact cggggaaga ttgcaagga 
      aattattgc aaaacatat ccaaaagcg gacaaaatt 
      ccgggtggt gttgggttt cgacgacga acttgattt 
      atttgacgg taatctgct tctctcaga gcaattgca 
      aacagtact agaaggtcc cagaaaaat ttatgttca 
      agacgtaac gccttacag acccgcaat aggagtcaa 
      ctcatttgt gtgcggtta ttacaatag cagcgacga 
      ttttgcgat tgtaccaat gaaggaatt gcaagaaca 
      cattgactg tattttaag atgtgatgt cgaagataa 
      tgtaccata cgttgttga tgtgaagga agtctgttt 
      gtgttattt tgtcgatca gtggtatag atgtgtagt 
      tacgaggat ttttgataa ggattgtga agttaggtt 
      actggattt tggtaatgt tacagttat cagaaaaga 
      aaatttaag gagacctga cgatgacat catgaaagt 
      gccaatttt ggctttacc gtgtaaact cactggtct 
      gccacatca aaatgttga agcagcgtt aaaaaagat 
      aattcttaa gggagagat tttcaaccg gttgatgta 
      aaatcaata acggggggt aaatgccac gtggaacta 
      ccnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnaact acctaaaat atacgcagc 
      tttgaaagg ctaggtcta acttagagc gatttttta 
      tcattgtat tcattgtgc actgctaaa caattctta 
      gcagctgta gctcaaaat gagtgattt gtttttatt 
      gtcgctttt atacgttaa gttttttta cgcctttta 
      tttgtaaac gaataaaat tacaagaat aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY