Status
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Gene information
General information
TC006821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450512.1: 173125..207006
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006821
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06740

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006821 RA
      MSKSSKSAH LDAVKQAVV ILMGSLARH LDKDDERIK 
PIVLRLIQA LSTPSQTVQ EAVGNCLPP LIPSVKDEA 
PGYINKLLH QLLKGDKYG ERKGAAYGL AGIVKGMGI 
LALKQHDIM TKLTEAIQD KKNYKHREG ALFAFEMLF 
HMLGKLFEP YIIHVLPHL LQCFGDTSQ YVRAAADDT 
AKVVMSKLS GHGVKLVLP SLLDGLEQD SWRTKTGSV 
ELLGAMAYC APKQLSSCL PSIVPKLIE VLSDSHMKV 
QEAGAAALK VIGSVIRNP EIQAIVPVL LKALQDPSN 
KTSVCLQTL LDTQFVHFI DAPSLALIM PVVQRAFMD 
RSTETRKMA AQIIGNMYS LTDQKDLLP YLPTIIPGL 
KTSLLDPVP EVRSVSARA LGAMVRGMG ESSFEDLLP 
WLMQTLTSE SSSVDRSGA AQGLSEVVG GLGVEKLHK 
LMPEIIATA ERTDIAPHV KDGYIMMFI YMPVVFTND 
FTVYIGQII NPILKALAD ENEYVRDTA LKAGQRIVT 
LYADSAILL LLPELEKGL FDENWRIRY SSVQLLGDL 
LYRISGVTG KMSTETASE DDNFGTEQS HHAIIKALG 
AERRNRVLA GLYMGRSDV ALMVRQAAL HVWKVVVTN 
TPRTLREIL PTLFGLLLG CLASDSYDK RQVAARTLG 
DLVRKLGER VLPEIIPIL ERGLQSDQA DQRQGVCIG 
LSEIMASTS KEMVLTFVN SLVPTVRKA LCDPLPEVR 
QAAAKTFDS LHSTVGARA LDDILPTML NQLNDSDPN 
VVEWTLDGL RQVMAIKSR VVLPYLVPQ LTAPPANTK 
ALSILASVA GEALNKYLP RILPALQTA LATSRGTPE 
EAQQLEYCQ AVVLSVSDE VGIRTVIDT MLENTRNEN 
ADQRRAAAT LLCGFCANS KAQYTTHVP QLLRGLIHL 
CTDSDKDVL QMSWEALNA VTKTLDPKL QATYVSDVR 
QAVRYAMSD LKGAQLLPG FCLPKGIAP ILPIFREAI 
LNGDGDEKE TAAQGLGEV IKVTSAQAL QPSVVAITG 
PLIRILGDR FSANVKTAV LETLAILLA KVGVMLKQF 
LPQLQTTFV KALNDPNRI VRLKSATAL SYLIVIHQR 
ADPLFMEMH NTIKQSDDS AIRETTLHA LRGIITPAG 
DKMSDTVKK QIHSTLLTL LGHQEDVTR NCAAGCLGA 
ICRWLTPEQ LEVTLTEHL LSEDVQIDW TLRHGRAAA 
LSVALKEFP ACIWQEQYK TRLIQTILG QLAADRVVI 
TQTAVRSCG YLLQFLMLN GEALPTNLL GPFVRTMNN 
NNNDVKQLL ARVCIHLAK TVPQEKMSS DLLKLLLPT 
LVNGTKEKN GYVKANSEL ALIAVLRLK DGDAMQQRC 
LSILDVGAR ESLADVVSK VLKKIMSQP EGKEEELDD 
TLLT
    CDS sequence
    • >TC006821 RA
      atgagcaaa tcgagcaaa tcggcccat ttggacgcc 
gtcaagcaa gctgtggta atcctcatg ggttcctta 
gcccgtcat ttagacaaa gacgatgaa agaatcaaa 
cctatagtt ttacgactt atacaagca ctatcaaca 
ccttcacaa acagtccag gaagcagtt ggcaattgc 
ttaccacct ctcatacca tcagtaaaa gacgaagct 
cctggttac ataaataaa ttactccat cagttatta 
aaaggcgat aaatacggc gaacgtaaa ggtgcagct 
tacggttta gctggaata gtaaaagga atgggaatt 
ctcgcttta aaacaacac gatattatg acaaaactc 
accgaagca atccaagac aagaaaaac tacaagcat 
cgagagggc gctttgttc gcctttgag atgcttttc 
cacatgttg ggcaaactt ttcgagccg tatattatc 
cacgttttg ccgcatttg ttgcaatgt ttcggcgat 
acgagtcag tatgttcgt gcagccgct gatgatacg 
gccaaagtt gtgatgagt aaactgtca ggacatgga 
gtcaagttg gttttgcct tcgttgctc gatggcttg 
gaacaggat tcctggagg actaagaca ggttcggtt 
gagttattg ggtgctatg gcgtattgt gcacccaaa 
caattgtcc tcgtgtttg ccgagtatt gtaccaaag 
ttgattgaa gtgttgagc gattcgcat atgaaagta 
caggaagct ggagctgcg gctttgaag gtgattgga 
tcggttata cgaaatccg gaaattcaa gccatcgtt 
cccgtacta ctcaaggcc cttcaagac ccttcaaac 
aaaacctcc gtgtgcctc caaaccctc cttgacacc 
caatttgtc cactttatt gacgcccca tccttggcc 
ctaataatg ccagtagtc caacgtgct ttcatggac 
cgttcgacc gaaacgcga aaaatggcc gcccaaatc 
atcggcaac atgtactca ctgaccgac caaaaagac 
ctccttccc tacctcccg accattata cccggcctt 
aaaacctct ctcctggat ccggtccca gaagtccgc 
agtgtgagc gctcgagct ttgggcgca atggttcga 
ggaatgggc gagtcgagc tttgaggac ttgctgcca 
tggttaatg caaacacta acgtctgaa agtagttca 
gttgatcgt tctggagct gcgcaaggt ctttccgag 
gtggttggt ggtttaggt gttgagaag ttgcacaag 
ttgatgccg gaaattatt gcaacggct gagaggact 
gatattgca ccgcatgtc aaggatgga tatatcatg 
atgtttatt tatatgccg gtggttttt accaatgat 
tttacggtg tatattgga cagattatt aatccgatt 
ttgaaagca ctagccgat gagaatgaa tatgtgagg 
gacaccgcc ttaaaggcg ggacaaagg attgtgacg 
ttgtatgcg gattcggcg attttgttg cttttgccc 
gagttggag aagggactg tttgatgag aattggagg 
attagatac agttcggtg caacttctc ggcgactta 
ctttaccgc atttctggc gttaccggc aaaatgagc 
actgaaaca gccagcgaa gacgacaat ttcggcacc 
gaacaatcc caccatgcc ataatcaaa gccctaggt 
gctgaacga cgtaatcgt gtcttggcc ggtctttac 
atgggccgt tccgacgtt gcactgatg gtccgacaa 
gcggctttg cacgtctgg aaggtcgta gtaacaaac 
acacctcga actttgcga gaaatttta ccaacattg 
ttcggtttg ttgctgggt tgtcttgcg agcgatagc 
tacgataag cgacaagtt gccgcaagg acacttggt 
gatctggtg cgcaaattg ggcgagagg gtactgccg 
gaaattatt ccgattctg gaaaggggg ctccagtcg 
gatcaagcc gatcagagg caaggtgtg tgtattggt 
ttgtcggaa attatggcg tcgacgtcc aaggagatg 
gttttgact tttgttaat tcgttggta ccgactgtg 
aggaaagca ttgtgtgat cctttgcca gaagtgagg 
caagccgca gcgaaaact ttcgatagt ttgcattcg 
acggttggt gcaagggct ttggatgat atcttaccg 
acgatgctt aatcaattg aacgactct gatcccaac 
gtggtcgaa tggacttta gacggcctc cgtcaagta 
atggcaatc aaatctcgc gttgtcctc ccttactta 
gtacctcaa ttaaccgcc cctcccgcc aacaccaaa 
gccctctcg attctggca tcagtagcc ggggaagcc 
ctcaacaaa tacctccct cgtattctc ccagcatta 
caaacagct ttggccaca tctcgtgga acaccagag 
gaagcccaa cagttggaa tactgtcaa gctgtcgtt 
ctatcggtc agtgacgaa gtcggtatt cgaaccgtc 
atcgacaca atgctcgaa aacacacga aacgaaaac 
gccgatcag agacgagcc gctgcgaca ctactttgc 
ggtttctgt gccaattca aaagcacag tatacaact 
catgtgcca caattgttg agaggtttg atacatttg 
tgtacggat agtgataag gatgtgttg caaatgtca 
tgggaggcg ttgaatgcg gttacgaag actttggat 
ccgaagttg caagcgact tatgtgtcg gatgttagg 
caagctgtg agatatgcc atgtcggat ttgaagggg 
gcgcagttg ttgcccgga ttttgttta cccaaaggt 
attgcccca attttgccc attttccgt gaggcaatc 
ctcaatggt gatggcgat gagaaagaa accgcagct 
caaggttta ggcgaagtg attaaagta acaagtgca 
caagccctt cagcctagc gttgttgca atcacaggc 
cctttgatt cgaatactt ggcgatcgt tttagtgcc 
aacgttaaa actgcagtt cttgaaact ttagcaatt 
ttattagct aaagttggg gtgatgtta aaacaattt 
ttaccacaa cttcaaaca acattcgtc aaggcttta 
aacgacccg aatcgaatc gtgcgcttg aaatcggcc 
acagcttta agttattta attgtaatc catcaaaga 
gcagatcca ctttttatg gaaatgcac aatacgatt 
aaacaaagt gacgattca gcaattagg gaaacaaca 
ttacatgcc ttaagaggg attattaca ccagctggt 
gataaaatg tccgataca gttaaaaaa cagattcat 
tcgactttg ttaacactt ttggggcat caggaagat 
gtgactagg aattgtgct gccggctgt ttgggggcg 
atttgtcgc tggttgaca ccagaacag ttggaagtt 
actctaact gagcatctt ttgagtgaa gatgtgcaa 
attgattgg actttgcgg catggaaga gcagcagct 
ttgtcggtg gctttgaag gaatttcca gcgtgtata 
tggcaagaa cagtacaaa acaagactg attcagacg 
attttgggg caattggct gctgataga gttgttatt 
acgcaaact gcagtgcgt tcgtgtggt tatttgctg 
cagtttttg atgctgaat ggggaagcg cttccgacg 
aatttgttg ggacctttt gtgaggaca atgaacaac 
aataacaac gatgtgaaa caactcttg gctcgagtt 
tgcatccat ttggctaaa accgtacca caggagaag 
atgtcttca gatttgttg aagctgctt ttaccaact 
ttagtcaac ggaactaaa gagaaaaat ggttatgtt 
aaggcaaac tcagaactt gcattgatt gcggttttg 
aggctaaaa gatggcgac gctatgcaa cagcgatgt 
ctgagtatt ctggacgtc ggcgctcgc gaatccctc 
gctgacgtc gtcagtaaa gtcctcaaa aagataatg 
tcgcagcct gagggaaag gaagaggag ctcgacgac 
actttactg acttga
    mRNA sequence
    • >TC006821 RA
      tacaggaaa ctgtcagta cattgtggc cagttttcg 
atactttta tgagcaaat cgagcaaat cggcccatt 
tggacgccg tcaagcaag ctgtggtaa tcctcatgg 
gttccttag cccgtcatt tagacaaag acgatgaaa 
gaatcaaac ctatagttt tacgactta tacaagcac 
tatcaacac cttcacaaa cagtccagg aagcagttg 
gcaattgct taccacctc tcataccat cagtaaaag 
acgaagctc ctggttaca taaataaat tactccatc 
agttattaa aaggcgata aatacggcg aacgtaaag 
gtgcagctt acggtttag ctggaatag taaaaggaa 
tgggaattc tcgctttaa aacaacacg atattatga 
caaaactca ccgaagcaa tccaagaca agaaaaact 
acaagcatc gagagggcg ctttgttcg cctttgaga 
tgcttttcc acatgttgg gcaaacttt tcgagccgt 
atattatcc acgttttgc cgcatttgt tgcaatgtt 
tcggcgata cgagtcagt atgttcgtg cagccgctg 
atgatacgg ccaaagttg tgatgagta aactgtcag 
gacatggag tcaagttgg ttttgcctt cgttgctcg 
atggcttgg aacaggatt cctggagga ctaagacag 
gttcggttg agttattgg gtgctatgg cgtattgtg 
cacccaaac aattgtcct cgtgtttgc cgagtattg 
taccaaagt tgattgaag tgttgagcg attcgcata 
tgaaagtac aggaagctg gagctgcgg ctttgaagg 
tgattggat cggttatac gaaatccgg aaattcaag 
ccatcgttc ccgtactac tcaaggccc ttcaagacc 
cttcaaaca aaacctccg tgtgcctcc aaaccctcc 
ttgacaccc aatttgtcc actttattg acgccccat 
ccttggccc taataatgc cagtagtcc aacgtgctt 
tcatggacc gttcgaccg aaacgcgaa aaatggccg 
cccaaatca tcggcaaca tgtactcac tgaccgacc 
aaaaagacc tccttccct acctcccga ccattatac 
ccggcctta aaacctctc tcctggatc cggtcccag 
aagtccgca gtgtgagcg ctcgagctt tgggcgcaa 
tggttcgag gaatgggcg agtcgagct ttgaggact 
tgctgccat ggttaatgc aaacactaa cgtctgaaa 
gtagttcag ttgatcgtt ctggagctg cgcaaggtc 
tttccgagg tggttggtg gtttaggtg ttgagaagt 
tgcacaagt tgatgccgg aaattattg caacggctg 
agaggactg atattgcac cgcatgtca aggatggat 
atatcatga tgtttattt atatgccgg tggttttta 
ccaatgatt ttacggtgt atattggac agattatta 
atccgattt tgaaagcac tagccgatg agaatgaat 
atgtgaggg acaccgcct taaaggcgg gacaaagga 
ttgtgacgt tgtatgcgg attcggcga ttttgttgc 
ttttgcccg agttggaga agggactgt ttgatgaga 
attggagga ttagataca gttcggtgc aacttctcg 
gcgacttac tttaccgca tttctggcg ttaccggca 
aaatgagca ctgaaacag ccagcgaag acgacaatt 
tcggcaccg aacaatccc accatgcca taatcaaag 
ccctaggtg ctgaacgac gtaatcgtg tcttggccg 
gtctttaca tgggccgtt ccgacgttg cactgatgg 
tccgacaag cggctttgc acgtctgga aggtcgtag 
taacaaaca cacctcgaa ctttgcgag aaattttac 
caacattgt tcggtttgt tgctgggtt gtcttgcga 
gcgatagct acgataagc gacaagttg ccgcaagga 
cacttggtg atctggtgc gcaaattgg gcgagaggg 
tactgccgg aaattattc cgattctgg aaagggggc 
tccagtcgg atcaagccg atcagaggc aaggtgtgt 
gtattggtt tgtcggaaa ttatggcgt cgacgtcca 
aggagatgg ttttgactt ttgttaatt cgttggtac 
cgactgtga ggaaagcat tgtgtgatc ctttgccag 
aagtgaggc aagccgcag cgaaaactt tcgatagtt 
tgcattcga cggttggtg caagggctt tggatgata 
tcttaccga cgatgctta atcaattga acgactctg 
atcccaacg tggtcgaat ggactttag acggcctcc 
gtcaagtaa tggcaatca aatctcgcg ttgtcctcc 
cttacttag tacctcaat taaccgccc ctcccgcca 
acaccaaag ccctctcga ttctggcat cagtagccg 
gggaagccc tcaacaaat acctccctc gtattctcc 
cagcattac aaacagctt tggccacat ctcgtggaa 
caccagagg aagcccaac agttggaat actgtcaag 
ctgtcgttc tatcggtca gtgacgaag tcggtattc 
gaaccgtca tcgacacaa tgctcgaaa acacacgaa 
acgaaaacg ccgatcaga gacgagccg ctgcgacac 
tactttgcg gtttctgtg ccaattcaa aagcacagt 
atacaactc atgtgccac aattgttga gaggtttga 
tacatttgt gtacggata gtgataagg atgtgttgc 
aaatgtcat gggaggcgt tgaatgcgg ttacgaaga 
ctttggatc cgaagttgc aagcgactt atgtgtcgg 
atgttaggc aagctgtga gatatgcca tgtcggatt 
tgaaggggg cgcagttgt tgcccggat tttgtttac 
ccaaaggta ttgccccaa ttttgccca ttttccgtg 
aggcaatcc tcaatggtg atggcgatg agaaagaaa 
ccgcagctc aaggtttag gcgaagtga ttaaagtaa 
caagtgcac aagcccttc agcctagcg ttgttgcaa 
tcacaggcc ctttgattc gaatacttg gcgatcgtt 
ttagtgcca acgttaaaa ctgcagttc ttgaaactt 
tagcaattt tattagcta aagttgggg tgatgttaa 
aacaatttt taccacaac ttcaaacaa cattcgtca 
aggctttaa acgacccga atcgaatcg tgcgcttga 
aatcggcca cagctttaa gttatttaa ttgtaatcc 
atcaaagag cagatccac tttttatgg aaatgcaca 
atacgatta aacaaagtg acgattcag caattaggg 
aaacaacat tacatgcct taagaggga ttattacac 
cagctggtg ataaaatgt ccgatacag ttaaaaaac 
agattcatt cgactttgt taacacttt tggggcatc 
aggaagatg tgactagga attgtgctg ccggctgtt 
tgggggcga tttgtcgct ggttgacac cagaacagt 
tggaagtta ctctaactg agcatcttt tgagtgaag 
atgtgcaaa ttgattgga ctttgcggc atggaagag 
cagcagctt tgtcggtgg ctttgaagg aatttccag 
cgtgtatat ggcaagaac agtacaaaa caagactga 
ttcagacga ttttggggc aattggctg ctgatagag 
ttgttatta cgcaaactg cagtgcgtt cgtgtggtt 
atttgctgc agtttttga tgctgaatg gggaagcgc 
ttccgacga atttgttgg gaccttttg tgaggacaa 
tgaacaaca ataacaacg atgtgaaac aactcttgg 
ctcgagttt gcatccatt tggctaaaa ccgtaccac 
aggagaaga tgtcttcag atttgttga agctgcttt 
taccaactt tagtcaacg gaactaaag agaaaaatg 
gttatgtta aggcaaact cagaacttg cattgattg 
cggttttga ggctaaaag atggcgacg ctatgcaac 
agcgatgtc tgagtattc tggacgtcg gcgctcgcg 
aatccctcg ctgacgtcg tcagtaaag tcctcaaaa 
agataatgt cgcagcctg agggaaagg aagaggagc 
tcgacgaca ctttactga cttgattat tagggtcac 
gcaacggag caaacaaca cgttgattt gttgtatcc 
ctgcgtttt tgatcattt ccggtgttc gtttcttca 
ttttgttcc ctttgagtt ttaatatat acgattttg 
taaa
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