Status
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Gene information
General information
TC006821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450512.1: 173125..207006
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006821
    iBeetle screen iB_06740

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006821 RA
      MSKSSKSAH LDAVKQAVV ILMGSLARH LDKDDERIK 
      PIVLRLIQA LSTPSQTVQ EAVGNCLPP LIPSVKDEA 
      PGYINKLLH QLLKGDKYG ERKGAAYGL AGIVKGMGI 
      LALKQHDIM TKLTEAIQD KKNYKHREG ALFAFEMLF 
      HMLGKLFEP YIIHVLPHL LQCFGDTSQ YVRAAADDT 
      AKVVMSKLS GHGVKLVLP SLLDGLEQD SWRTKTGSV 
      ELLGAMAYC APKQLSSCL PSIVPKLIE VLSDSHMKV 
      QEAGAAALK VIGSVIRNP EIQAIVPVL LKALQDPSN 
      KTSVCLQTL LDTQFVHFI DAPSLALIM PVVQRAFMD 
      RSTETRKMA AQIIGNMYS LTDQKDLLP YLPTIIPGL 
      KTSLLDPVP EVRSVSARA LGAMVRGMG ESSFEDLLP 
      WLMQTLTSE SSSVDRSGA AQGLSEVVG GLGVEKLHK 
      LMPEIIATA ERTDIAPHV KDGYIMMFI YMPVVFTND 
      FTVYIGQII NPILKALAD ENEYVRDTA LKAGQRIVT 
      LYADSAILL LLPELEKGL FDENWRIRY SSVQLLGDL 
      LYRISGVTG KMSTETASE DDNFGTEQS HHAIIKALG 
      AERRNRVLA GLYMGRSDV ALMVRQAAL HVWKVVVTN 
      TPRTLREIL PTLFGLLLG CLASDSYDK RQVAARTLG 
      DLVRKLGER VLPEIIPIL ERGLQSDQA DQRQGVCIG 
      LSEIMASTS KEMVLTFVN SLVPTVRKA LCDPLPEVR 
      QAAAKTFDS LHSTVGARA LDDILPTML NQLNDSDPN 
      VVEWTLDGL RQVMAIKSR VVLPYLVPQ LTAPPANTK 
      ALSILASVA GEALNKYLP RILPALQTA LATSRGTPE 
      EAQQLEYCQ AVVLSVSDE VGIRTVIDT MLENTRNEN 
      ADQRRAAAT LLCGFCANS KAQYTTHVP QLLRGLIHL 
      CTDSDKDVL QMSWEALNA VTKTLDPKL QATYVSDVR 
      QAVRYAMSD LKGAQLLPG FCLPKGIAP ILPIFREAI 
      LNGDGDEKE TAAQGLGEV IKVTSAQAL QPSVVAITG 
      PLIRILGDR FSANVKTAV LETLAILLA KVGVMLKQF 
      LPQLQTTFV KALNDPNRI VRLKSATAL SYLIVIHQR 
      ADPLFMEMH NTIKQSDDS AIRETTLHA LRGIITPAG 
      DKMSDTVKK QIHSTLLTL LGHQEDVTR NCAAGCLGA 
      ICRWLTPEQ LEVTLTEHL LSEDVQIDW TLRHGRAAA 
      LSVALKEFP ACIWQEQYK TRLIQTILG QLAADRVVI 
      TQTAVRSCG YLLQFLMLN GEALPTNLL GPFVRTMNN 
      NNNDVKQLL ARVCIHLAK TVPQEKMSS DLLKLLLPT 
      LVNGTKEKN GYVKANSEL ALIAVLRLK DGDAMQQRC 
      LSILDVGAR ESLADVVSK VLKKIMSQP EGKEEELDD 
      TLLT
    CDS sequence
    • >TC006821 RA
      atgagcaaa tcgagcaaa tcggcccat ttggacgcc 
      gtcaagcaa gctgtggta atcctcatg ggttcctta 
      gcccgtcat ttagacaaa gacgatgaa agaatcaaa 
      cctatagtt ttacgactt atacaagca ctatcaaca 
      ccttcacaa acagtccag gaagcagtt ggcaattgc 
      ttaccacct ctcatacca tcagtaaaa gacgaagct 
      cctggttac ataaataaa ttactccat cagttatta 
      aaaggcgat aaatacggc gaacgtaaa ggtgcagct 
      tacggttta gctggaata gtaaaagga atgggaatt 
      ctcgcttta aaacaacac gatattatg acaaaactc 
      accgaagca atccaagac aagaaaaac tacaagcat 
      cgagagggc gctttgttc gcctttgag atgcttttc 
      cacatgttg ggcaaactt ttcgagccg tatattatc 
      cacgttttg ccgcatttg ttgcaatgt ttcggcgat 
      acgagtcag tatgttcgt gcagccgct gatgatacg 
      gccaaagtt gtgatgagt aaactgtca ggacatgga 
      gtcaagttg gttttgcct tcgttgctc gatggcttg 
      gaacaggat tcctggagg actaagaca ggttcggtt 
      gagttattg ggtgctatg gcgtattgt gcacccaaa 
      caattgtcc tcgtgtttg ccgagtatt gtaccaaag 
      ttgattgaa gtgttgagc gattcgcat atgaaagta 
      caggaagct ggagctgcg gctttgaag gtgattgga 
      tcggttata cgaaatccg gaaattcaa gccatcgtt 
      cccgtacta ctcaaggcc cttcaagac ccttcaaac 
      aaaacctcc gtgtgcctc caaaccctc cttgacacc 
      caatttgtc cactttatt gacgcccca tccttggcc 
      ctaataatg ccagtagtc caacgtgct ttcatggac 
      cgttcgacc gaaacgcga aaaatggcc gcccaaatc 
      atcggcaac atgtactca ctgaccgac caaaaagac 
      ctccttccc tacctcccg accattata cccggcctt 
      aaaacctct ctcctggat ccggtccca gaagtccgc 
      agtgtgagc gctcgagct ttgggcgca atggttcga 
      ggaatgggc gagtcgagc tttgaggac ttgctgcca 
      tggttaatg caaacacta acgtctgaa agtagttca 
      gttgatcgt tctggagct gcgcaaggt ctttccgag 
      gtggttggt ggtttaggt gttgagaag ttgcacaag 
      ttgatgccg gaaattatt gcaacggct gagaggact 
      gatattgca ccgcatgtc aaggatgga tatatcatg 
      atgtttatt tatatgccg gtggttttt accaatgat 
      tttacggtg tatattgga cagattatt aatccgatt 
      ttgaaagca ctagccgat gagaatgaa tatgtgagg 
      gacaccgcc ttaaaggcg ggacaaagg attgtgacg 
      ttgtatgcg gattcggcg attttgttg cttttgccc 
      gagttggag aagggactg tttgatgag aattggagg 
      attagatac agttcggtg caacttctc ggcgactta 
      ctttaccgc atttctggc gttaccggc aaaatgagc 
      actgaaaca gccagcgaa gacgacaat ttcggcacc 
      gaacaatcc caccatgcc ataatcaaa gccctaggt 
      gctgaacga cgtaatcgt gtcttggcc ggtctttac 
      atgggccgt tccgacgtt gcactgatg gtccgacaa 
      gcggctttg cacgtctgg aaggtcgta gtaacaaac 
      acacctcga actttgcga gaaatttta ccaacattg 
      ttcggtttg ttgctgggt tgtcttgcg agcgatagc 
      tacgataag cgacaagtt gccgcaagg acacttggt 
      gatctggtg cgcaaattg ggcgagagg gtactgccg 
      gaaattatt ccgattctg gaaaggggg ctccagtcg 
      gatcaagcc gatcagagg caaggtgtg tgtattggt 
      ttgtcggaa attatggcg tcgacgtcc aaggagatg 
      gttttgact tttgttaat tcgttggta ccgactgtg 
      aggaaagca ttgtgtgat cctttgcca gaagtgagg 
      caagccgca gcgaaaact ttcgatagt ttgcattcg 
      acggttggt gcaagggct ttggatgat atcttaccg 
      acgatgctt aatcaattg aacgactct gatcccaac 
      gtggtcgaa tggacttta gacggcctc cgtcaagta 
      atggcaatc aaatctcgc gttgtcctc ccttactta 
      gtacctcaa ttaaccgcc cctcccgcc aacaccaaa 
      gccctctcg attctggca tcagtagcc ggggaagcc 
      ctcaacaaa tacctccct cgtattctc ccagcatta 
      caaacagct ttggccaca tctcgtgga acaccagag 
      gaagcccaa cagttggaa tactgtcaa gctgtcgtt 
      ctatcggtc agtgacgaa gtcggtatt cgaaccgtc 
      atcgacaca atgctcgaa aacacacga aacgaaaac 
      gccgatcag agacgagcc gctgcgaca ctactttgc 
      ggtttctgt gccaattca aaagcacag tatacaact 
      catgtgcca caattgttg agaggtttg atacatttg 
      tgtacggat agtgataag gatgtgttg caaatgtca 
      tgggaggcg ttgaatgcg gttacgaag actttggat 
      ccgaagttg caagcgact tatgtgtcg gatgttagg 
      caagctgtg agatatgcc atgtcggat ttgaagggg 
      gcgcagttg ttgcccgga ttttgttta cccaaaggt 
      attgcccca attttgccc attttccgt gaggcaatc 
      ctcaatggt gatggcgat gagaaagaa accgcagct 
      caaggttta ggcgaagtg attaaagta acaagtgca 
      caagccctt cagcctagc gttgttgca atcacaggc 
      cctttgatt cgaatactt ggcgatcgt tttagtgcc 
      aacgttaaa actgcagtt cttgaaact ttagcaatt 
      ttattagct aaagttggg gtgatgtta aaacaattt 
      ttaccacaa cttcaaaca acattcgtc aaggcttta 
      aacgacccg aatcgaatc gtgcgcttg aaatcggcc 
      acagcttta agttattta attgtaatc catcaaaga 
      gcagatcca ctttttatg gaaatgcac aatacgatt 
      aaacaaagt gacgattca gcaattagg gaaacaaca 
      ttacatgcc ttaagaggg attattaca ccagctggt 
      gataaaatg tccgataca gttaaaaaa cagattcat 
      tcgactttg ttaacactt ttggggcat caggaagat 
      gtgactagg aattgtgct gccggctgt ttgggggcg 
      atttgtcgc tggttgaca ccagaacag ttggaagtt 
      actctaact gagcatctt ttgagtgaa gatgtgcaa 
      attgattgg actttgcgg catggaaga gcagcagct 
      ttgtcggtg gctttgaag gaatttcca gcgtgtata 
      tggcaagaa cagtacaaa acaagactg attcagacg 
      attttgggg caattggct gctgataga gttgttatt 
      acgcaaact gcagtgcgt tcgtgtggt tatttgctg 
      cagtttttg atgctgaat ggggaagcg cttccgacg 
      aatttgttg ggacctttt gtgaggaca atgaacaac 
      aataacaac gatgtgaaa caactcttg gctcgagtt 
      tgcatccat ttggctaaa accgtacca caggagaag 
      atgtcttca gatttgttg aagctgctt ttaccaact 
      ttagtcaac ggaactaaa gagaaaaat ggttatgtt 
      aaggcaaac tcagaactt gcattgatt gcggttttg 
      aggctaaaa gatggcgac gctatgcaa cagcgatgt 
      ctgagtatt ctggacgtc ggcgctcgc gaatccctc 
      gctgacgtc gtcagtaaa gtcctcaaa aagataatg 
      tcgcagcct gagggaaag gaagaggag ctcgacgac 
      actttactg acttga
    mRNA sequence
    • >TC006821 RA
      tacaggaaa ctgtcagta cattgtggc cagttttcg 
      atactttta tgagcaaat cgagcaaat cggcccatt 
      tggacgccg tcaagcaag ctgtggtaa tcctcatgg 
      gttccttag cccgtcatt tagacaaag acgatgaaa 
      gaatcaaac ctatagttt tacgactta tacaagcac 
      tatcaacac cttcacaaa cagtccagg aagcagttg 
      gcaattgct taccacctc tcataccat cagtaaaag 
      acgaagctc ctggttaca taaataaat tactccatc 
      agttattaa aaggcgata aatacggcg aacgtaaag 
      gtgcagctt acggtttag ctggaatag taaaaggaa 
      tgggaattc tcgctttaa aacaacacg atattatga 
      caaaactca ccgaagcaa tccaagaca agaaaaact 
      acaagcatc gagagggcg ctttgttcg cctttgaga 
      tgcttttcc acatgttgg gcaaacttt tcgagccgt 
      atattatcc acgttttgc cgcatttgt tgcaatgtt 
      tcggcgata cgagtcagt atgttcgtg cagccgctg 
      atgatacgg ccaaagttg tgatgagta aactgtcag 
      gacatggag tcaagttgg ttttgcctt cgttgctcg 
      atggcttgg aacaggatt cctggagga ctaagacag 
      gttcggttg agttattgg gtgctatgg cgtattgtg 
      cacccaaac aattgtcct cgtgtttgc cgagtattg 
      taccaaagt tgattgaag tgttgagcg attcgcata 
      tgaaagtac aggaagctg gagctgcgg ctttgaagg 
      tgattggat cggttatac gaaatccgg aaattcaag 
      ccatcgttc ccgtactac tcaaggccc ttcaagacc 
      cttcaaaca aaacctccg tgtgcctcc aaaccctcc 
      ttgacaccc aatttgtcc actttattg acgccccat 
      ccttggccc taataatgc cagtagtcc aacgtgctt 
      tcatggacc gttcgaccg aaacgcgaa aaatggccg 
      cccaaatca tcggcaaca tgtactcac tgaccgacc 
      aaaaagacc tccttccct acctcccga ccattatac 
      ccggcctta aaacctctc tcctggatc cggtcccag 
      aagtccgca gtgtgagcg ctcgagctt tgggcgcaa 
      tggttcgag gaatgggcg agtcgagct ttgaggact 
      tgctgccat ggttaatgc aaacactaa cgtctgaaa 
      gtagttcag ttgatcgtt ctggagctg cgcaaggtc 
      tttccgagg tggttggtg gtttaggtg ttgagaagt 
      tgcacaagt tgatgccgg aaattattg caacggctg 
      agaggactg atattgcac cgcatgtca aggatggat 
      atatcatga tgtttattt atatgccgg tggttttta 
      ccaatgatt ttacggtgt atattggac agattatta 
      atccgattt tgaaagcac tagccgatg agaatgaat 
      atgtgaggg acaccgcct taaaggcgg gacaaagga 
      ttgtgacgt tgtatgcgg attcggcga ttttgttgc 
      ttttgcccg agttggaga agggactgt ttgatgaga 
      attggagga ttagataca gttcggtgc aacttctcg 
      gcgacttac tttaccgca tttctggcg ttaccggca 
      aaatgagca ctgaaacag ccagcgaag acgacaatt 
      tcggcaccg aacaatccc accatgcca taatcaaag 
      ccctaggtg ctgaacgac gtaatcgtg tcttggccg 
      gtctttaca tgggccgtt ccgacgttg cactgatgg 
      tccgacaag cggctttgc acgtctgga aggtcgtag 
      taacaaaca cacctcgaa ctttgcgag aaattttac 
      caacattgt tcggtttgt tgctgggtt gtcttgcga 
      gcgatagct acgataagc gacaagttg ccgcaagga 
      cacttggtg atctggtgc gcaaattgg gcgagaggg 
      tactgccgg aaattattc cgattctgg aaagggggc 
      tccagtcgg atcaagccg atcagaggc aaggtgtgt 
      gtattggtt tgtcggaaa ttatggcgt cgacgtcca 
      aggagatgg ttttgactt ttgttaatt cgttggtac 
      cgactgtga ggaaagcat tgtgtgatc ctttgccag 
      aagtgaggc aagccgcag cgaaaactt tcgatagtt 
      tgcattcga cggttggtg caagggctt tggatgata 
      tcttaccga cgatgctta atcaattga acgactctg 
      atcccaacg tggtcgaat ggactttag acggcctcc 
      gtcaagtaa tggcaatca aatctcgcg ttgtcctcc 
      cttacttag tacctcaat taaccgccc ctcccgcca 
      acaccaaag ccctctcga ttctggcat cagtagccg 
      gggaagccc tcaacaaat acctccctc gtattctcc 
      cagcattac aaacagctt tggccacat ctcgtggaa 
      caccagagg aagcccaac agttggaat actgtcaag 
      ctgtcgttc tatcggtca gtgacgaag tcggtattc 
      gaaccgtca tcgacacaa tgctcgaaa acacacgaa 
      acgaaaacg ccgatcaga gacgagccg ctgcgacac 
      tactttgcg gtttctgtg ccaattcaa aagcacagt 
      atacaactc atgtgccac aattgttga gaggtttga 
      tacatttgt gtacggata gtgataagg atgtgttgc 
      aaatgtcat gggaggcgt tgaatgcgg ttacgaaga 
      ctttggatc cgaagttgc aagcgactt atgtgtcgg 
      atgttaggc aagctgtga gatatgcca tgtcggatt 
      tgaaggggg cgcagttgt tgcccggat tttgtttac 
      ccaaaggta ttgccccaa ttttgccca ttttccgtg 
      aggcaatcc tcaatggtg atggcgatg agaaagaaa 
      ccgcagctc aaggtttag gcgaagtga ttaaagtaa 
      caagtgcac aagcccttc agcctagcg ttgttgcaa 
      tcacaggcc ctttgattc gaatacttg gcgatcgtt 
      ttagtgcca acgttaaaa ctgcagttc ttgaaactt 
      tagcaattt tattagcta aagttgggg tgatgttaa 
      aacaatttt taccacaac ttcaaacaa cattcgtca 
      aggctttaa acgacccga atcgaatcg tgcgcttga 
      aatcggcca cagctttaa gttatttaa ttgtaatcc 
      atcaaagag cagatccac tttttatgg aaatgcaca 
      atacgatta aacaaagtg acgattcag caattaggg 
      aaacaacat tacatgcct taagaggga ttattacac 
      cagctggtg ataaaatgt ccgatacag ttaaaaaac 
      agattcatt cgactttgt taacacttt tggggcatc 
      aggaagatg tgactagga attgtgctg ccggctgtt 
      tgggggcga tttgtcgct ggttgacac cagaacagt 
      tggaagtta ctctaactg agcatcttt tgagtgaag 
      atgtgcaaa ttgattgga ctttgcggc atggaagag 
      cagcagctt tgtcggtgg ctttgaagg aatttccag 
      cgtgtatat ggcaagaac agtacaaaa caagactga 
      ttcagacga ttttggggc aattggctg ctgatagag 
      ttgttatta cgcaaactg cagtgcgtt cgtgtggtt 
      atttgctgc agtttttga tgctgaatg gggaagcgc 
      ttccgacga atttgttgg gaccttttg tgaggacaa 
      tgaacaaca ataacaacg atgtgaaac aactcttgg 
      ctcgagttt gcatccatt tggctaaaa ccgtaccac 
      aggagaaga tgtcttcag atttgttga agctgcttt 
      taccaactt tagtcaacg gaactaaag agaaaaatg 
      gttatgtta aggcaaact cagaacttg cattgattg 
      cggttttga ggctaaaag atggcgacg ctatgcaac 
      agcgatgtc tgagtattc tggacgtcg gcgctcgcg 
      aatccctcg ctgacgtcg tcagtaaag tcctcaaaa 
      agataatgt cgcagcctg agggaaagg aagaggagc 
      tcgacgaca ctttactga cttgattat tagggtcac 
      gcaacggag caaacaaca cgttgattt gttgtatcc 
      ctgcgtttt tgatcattt ccggtgttc gtttcttca 
      ttttgttcc ctttgagtt ttaatatat acgattttg 
      taaa
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