General information | |||
TC031831
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450289.1: 70572..82753 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031831 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06735 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03280 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031831 RA
MQTFVTVVS ELWGNQCIR LKVTVKSDN CGESEDCSG KGICYSNDS MDGYECQCC LGFVGQHCE EQDACYPSR CKNNGICVD ISQGHEGST FQCLCPYGY TGKTCEDQT NACLSMPCQ NGATCIGNV TQFKCECLT GFSGPLCQH SHNDCESSP CVHGICVNQ EEGYKCFCQ PGFTGTHCE YEYNECQSS PCTNGGTCT DHVGHYTCT CGRGFTGRR CHIKVDLCQ PNPCPEPRY CVDHGNNYS CECPKGYSG PDCLIPSRA ACSTNPCTN GGTCWSNMD SFYCACKPG YTGKTCEDE FVVETITNV DTINKENEL DLQMPISIH LDHLHNVYI AAGTLACAF VIVALIVTV CHCRVNKTY KRFISRAYP LWGCRKRVS QPSASTNSE WFSGKDKAA TATPSRSLP SLDATDMYY TLDFSESQC SPLIQ
- >TC031831 RA
atgcaaaca ttcgttaca gttgtctcg gaattgtgg gggaatcag tgtattcga ttgaaagtc actgtgaaa tctgacaac tgtggggag agcgaagac tgctccggg aaaggcatc tgttattcc aacgattcg atggacgga tatgagtgc caatgctgt cttggtttt gtcggccaa cattgcgaa gagcaggac gcttgttat ccgtctcgg tgcaaaaat aatggaatc tgtgtggat atttcccaa ggacatgaa ggatcgact tttcaatgc ctgtgtcct tatggttat accggaaaa acgtgcgaa gatcaaacg aatgcctgc ctatcgatg ccttgccag aacggcgct acgtgcatc gggaatgtc acccagttt aaatgcgag tgtcttacg ggattttca ggaccgctg tgccaacac agccacaac gactgcgaa tcctcacct tgcgtccac gggatatgt gtcaatcaa gaggaaggg tataaatgc ttttgccag ccaggattt actggaact cactgcgag tacgagtat aatgaatgt caatcgtct ccgtgcaca aatgggggc acttgtacc gatcacgtg ggacattat acgtgtact tgtggccgg ggctttacc gggcggagg tgtcacatt aaggtggac ctttgccag cccaatcct tgccctgag ccacgctac tgcgtggac cacggcaat aactactcc tgcgagtgt cctaaaggc tattcgggc cccgattgt ttaattcca tctcgagcg gcttgcagc accaatccc tgcacgaac gggggaact tgctggagc aacatggac tcattttat tgtgcttgt aaacccggc tacactggc aaaacttgc gaagatgag tttgttgtt gaaacaata acgaacgtt gatactatc aacaaagag aatgaactc gatctgcaa atgccgatc tctattcat cttgatcat ctgcataat gtttatatc gctgctgga acgctagcc tgcgccttt gttattgtg gcactcatc gtgacagtt tgccactgc cgggtgaac aaaacgtat aagcgtttt atttcgcgg gcgtatccg ctgtggggt tgcaggaag agggtgtcc cagccgtcg gcctcgacc aatagcgaa tggtttagc gggaaggac aaggcggcc acggcaact cctagtcgc agtttaccg tcgttggat gcgactgat atgtactac actttggat ttcagcgag agtcaatgc tcgccactg atccagtaa
- >TC031831 RA
tgcacgaaa cgagcaaaa ttctggtca aaaacgtca gcccgagtt tcattaaaa gaggaggca acagcttga gcacttagg tggagttcg aaatttcta aataaaaat gtggagtgt gcggtttaa atctgatga agtagtgca ataaatgca aacattcgt tacagttgt ctcggaatt gtgggggaa tcagtgtat tcgattgaa agtcactgt gaaatctga caactgtgg ggagagcga agactgctc cgggaaagg catctgtta ttccaacga ttcgatgga cggatatga gtgccaatg ctgtcttgg ttttgtcgg ccaacattg cgaagagca ggacgcttg ttatccgtc tcggtgcaa aaataatgg aatctgtgt ggatatttc ccaaggaca tgaaggatc gacttttca atgcctgtg tccttatgg ttataccgg aaaaacgtg cgaagatca aacgaatgc ctgcctatc gatgccttg ccagaacgg cgctacgtg catcgggaa tgtcaccca gtttaaatg cgagtgtct tacgggatt ttcaggacc gctgtgcca acacagcca caacgactg cgaatcctc accttgcgt ccacgggat atgtgtcaa tcaagagga agggtataa atgcttttg ccagccagg atttactgg aactcactg cgagtacga gtataatga atgtcaatc gtctccgtg cacaaatgg gggcacttg taccgatca cgtgggaca ttatacgtg tacttgtgg ccggggctt taccgggcg gaggtgtca cattaaggt ggacctttg ccagcccaa tccttgccc tgagccacg ctactgcgt ggaccacgg caataacta ctcctgcga gtgtcctaa aggctattc gggccccga ttgtttaat tccatctcg agcggcttg cagcaccaa tccctgcac gaacggggg aacttgctg gagcaacat ggactcatt ttattgtgc ttgtaaacc cggctacac tggcaaaac ttgcgaaga tgagtttgt tgttgaaac aataacgaa cgttgatac tatcaacaa agagaatga actcgatct gcaaatgcc gatctctat tcatcttga tcatctgca taatgttta tatcgctgc tggaacgct agcctgcgc ctttgttat tgtggcact catcgtgac agtttgcca ctgccgggt gaacaaaac gtataagcg ttttatttc gcgggcgta tccgctgtg gggttgcag gaagagggt gtcccagcc gtcggcctc gaccaatag cgaatggtt tagcgggaa ggacaaggc ggccacggc aactcctag tcgcagttt accgtcgtt ggatgcgac tgatatgta ctacacttt ggatttcag cgagagtca atgctcgcc actgatcca gtaattggc ttgtaattg tagattttg taagccgtt ttacaaaag tttatttcc aactcgata tcacattca gtgttattt gtgttactt gtagctttt ttgtgtgga atcatcgct gcatttact cgttaccct gatgcacca acaaaattt attgccgtt tctacacgt ttattgcgt atttatatg tacatacaa aataatact caaatgaag aacgaattt gctgaagga ggtactcat tgttgattg aatcgactt agatttgga gttttattt aggcaaaaa gttgtttcg ttatagttt taaagtata aacattgct caaagtgct ttgcgaaat tttgatagt agtcattga gcaattata tatacaaaa agacagtaa ttgttagat tttttgaca tgtgctcaa cctagtctt aataagtac ttgcatttt gtagcagat attacgaaa tgtttcata gtctcgatt gctttaatt tggtgataa tttgctgat gctgaaccg aagtgttgt aaagtatga acctaaccg aacctaacc taaccatgt gccaaatat agaatgtat gtagtttaa gtatcactt gtatctcga ctttatcat ataaatggg gctttaaaa attgttact gtaaataaa acttattat gtagatgtt tctactttc cacagtgca atgccattc catccatat aatcgatca cttgttcat caccaatct gtttttaaa gcatatgat attggaaat ttcctgttt ctaataaag tatgtctaa aaag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
