Status
Please wait ...
Gene information
General information
TC031831
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001967
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 70572..82753
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031831
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06735

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03280

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031831 RA
      MQTFVTVVS ELWGNQCIR LKVTVKSDN CGESEDCSG 
      KGICYSNDS MDGYECQCC LGFVGQHCE EQDACYPSR 
      CKNNGICVD ISQGHEGST FQCLCPYGY TGKTCEDQT 
      NACLSMPCQ NGATCIGNV TQFKCECLT GFSGPLCQH 
      SHNDCESSP CVHGICVNQ EEGYKCFCQ PGFTGTHCE 
      YEYNECQSS PCTNGGTCT DHVGHYTCT CGRGFTGRR 
      CHIKVDLCQ PNPCPEPRY CVDHGNNYS CECPKGYSG 
      PDCLIPSRA ACSTNPCTN GGTCWSNMD SFYCACKPG 
      YTGKTCEDE FVVETITNV DTINKENEL DLQMPISIH 
      LDHLHNVYI AAGTLACAF VIVALIVTV CHCRVNKTY 
      KRFISRAYP LWGCRKRVS QPSASTNSE WFSGKDKAA 
      TATPSRSLP SLDATDMYY TLDFSESQC SPLIQ
    CDS sequence
    • >TC031831 RA
      atgcaaaca ttcgttaca gttgtctcg gaattgtgg 
      gggaatcag tgtattcga ttgaaagtc actgtgaaa 
      tctgacaac tgtggggag agcgaagac tgctccggg 
      aaaggcatc tgttattcc aacgattcg atggacgga 
      tatgagtgc caatgctgt cttggtttt gtcggccaa 
      cattgcgaa gagcaggac gcttgttat ccgtctcgg 
      tgcaaaaat aatggaatc tgtgtggat atttcccaa 
      ggacatgaa ggatcgact tttcaatgc ctgtgtcct 
      tatggttat accggaaaa acgtgcgaa gatcaaacg 
      aatgcctgc ctatcgatg ccttgccag aacggcgct 
      acgtgcatc gggaatgtc acccagttt aaatgcgag 
      tgtcttacg ggattttca ggaccgctg tgccaacac 
      agccacaac gactgcgaa tcctcacct tgcgtccac 
      gggatatgt gtcaatcaa gaggaaggg tataaatgc 
      ttttgccag ccaggattt actggaact cactgcgag 
      tacgagtat aatgaatgt caatcgtct ccgtgcaca 
      aatgggggc acttgtacc gatcacgtg ggacattat 
      acgtgtact tgtggccgg ggctttacc gggcggagg 
      tgtcacatt aaggtggac ctttgccag cccaatcct 
      tgccctgag ccacgctac tgcgtggac cacggcaat 
      aactactcc tgcgagtgt cctaaaggc tattcgggc 
      cccgattgt ttaattcca tctcgagcg gcttgcagc 
      accaatccc tgcacgaac gggggaact tgctggagc 
      aacatggac tcattttat tgtgcttgt aaacccggc 
      tacactggc aaaacttgc gaagatgag tttgttgtt 
      gaaacaata acgaacgtt gatactatc aacaaagag 
      aatgaactc gatctgcaa atgccgatc tctattcat 
      cttgatcat ctgcataat gtttatatc gctgctgga 
      acgctagcc tgcgccttt gttattgtg gcactcatc 
      gtgacagtt tgccactgc cgggtgaac aaaacgtat 
      aagcgtttt atttcgcgg gcgtatccg ctgtggggt 
      tgcaggaag agggtgtcc cagccgtcg gcctcgacc 
      aatagcgaa tggtttagc gggaaggac aaggcggcc 
      acggcaact cctagtcgc agtttaccg tcgttggat 
      gcgactgat atgtactac actttggat ttcagcgag 
      agtcaatgc tcgccactg atccagtaa
    mRNA sequence
    • >TC031831 RA
      tgcacgaaa cgagcaaaa ttctggtca aaaacgtca 
      gcccgagtt tcattaaaa gaggaggca acagcttga 
      gcacttagg tggagttcg aaatttcta aataaaaat 
      gtggagtgt gcggtttaa atctgatga agtagtgca 
      ataaatgca aacattcgt tacagttgt ctcggaatt 
      gtgggggaa tcagtgtat tcgattgaa agtcactgt 
      gaaatctga caactgtgg ggagagcga agactgctc 
      cgggaaagg catctgtta ttccaacga ttcgatgga 
      cggatatga gtgccaatg ctgtcttgg ttttgtcgg 
      ccaacattg cgaagagca ggacgcttg ttatccgtc 
      tcggtgcaa aaataatgg aatctgtgt ggatatttc 
      ccaaggaca tgaaggatc gacttttca atgcctgtg 
      tccttatgg ttataccgg aaaaacgtg cgaagatca 
      aacgaatgc ctgcctatc gatgccttg ccagaacgg 
      cgctacgtg catcgggaa tgtcaccca gtttaaatg 
      cgagtgtct tacgggatt ttcaggacc gctgtgcca 
      acacagcca caacgactg cgaatcctc accttgcgt 
      ccacgggat atgtgtcaa tcaagagga agggtataa 
      atgcttttg ccagccagg atttactgg aactcactg 
      cgagtacga gtataatga atgtcaatc gtctccgtg 
      cacaaatgg gggcacttg taccgatca cgtgggaca 
      ttatacgtg tacttgtgg ccggggctt taccgggcg 
      gaggtgtca cattaaggt ggacctttg ccagcccaa 
      tccttgccc tgagccacg ctactgcgt ggaccacgg 
      caataacta ctcctgcga gtgtcctaa aggctattc 
      gggccccga ttgtttaat tccatctcg agcggcttg 
      cagcaccaa tccctgcac gaacggggg aacttgctg 
      gagcaacat ggactcatt ttattgtgc ttgtaaacc 
      cggctacac tggcaaaac ttgcgaaga tgagtttgt 
      tgttgaaac aataacgaa cgttgatac tatcaacaa 
      agagaatga actcgatct gcaaatgcc gatctctat 
      tcatcttga tcatctgca taatgttta tatcgctgc 
      tggaacgct agcctgcgc ctttgttat tgtggcact 
      catcgtgac agtttgcca ctgccgggt gaacaaaac 
      gtataagcg ttttatttc gcgggcgta tccgctgtg 
      gggttgcag gaagagggt gtcccagcc gtcggcctc 
      gaccaatag cgaatggtt tagcgggaa ggacaaggc 
      ggccacggc aactcctag tcgcagttt accgtcgtt 
      ggatgcgac tgatatgta ctacacttt ggatttcag 
      cgagagtca atgctcgcc actgatcca gtaattggc 
      ttgtaattg tagattttg taagccgtt ttacaaaag 
      tttatttcc aactcgata tcacattca gtgttattt 
      gtgttactt gtagctttt ttgtgtgga atcatcgct 
      gcatttact cgttaccct gatgcacca acaaaattt 
      attgccgtt tctacacgt ttattgcgt atttatatg 
      tacatacaa aataatact caaatgaag aacgaattt 
      gctgaagga ggtactcat tgttgattg aatcgactt 
      agatttgga gttttattt aggcaaaaa gttgtttcg 
      ttatagttt taaagtata aacattgct caaagtgct 
      ttgcgaaat tttgatagt agtcattga gcaattata 
      tatacaaaa agacagtaa ttgttagat tttttgaca 
      tgtgctcaa cctagtctt aataagtac ttgcatttt 
      gtagcagat attacgaaa tgtttcata gtctcgatt 
      gctttaatt tggtgataa tttgctgat gctgaaccg 
      aagtgttgt aaagtatga acctaaccg aacctaacc 
      taaccatgt gccaaatat agaatgtat gtagtttaa 
      gtatcactt gtatctcga ctttatcat ataaatggg 
      gctttaaaa attgttact gtaaataaa acttattat 
      gtagatgtt tctactttc cacagtgca atgccattc 
      catccatat aatcgatca cttgttcat caccaatct 
      gtttttaaa gcatatgat attggaaat ttcctgttt 
      ctaataaag tatgtctaa aaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY