Status
Please wait ...
Gene information
General information
TC031844
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001410,TC001411 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451510.1: 20716..60613
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031844
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    iBeetle screen iB_06734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031844 RA
      MPAPELNGP SSTNGSPLS TDRFTVFQS SQPISVRAT 
YGPFSTKQT VPARYVVPD PLPLNTTGP AIDLQDLAT 
HHLDMSAHI VRNEIPRDS PVLRVLFHT GTDPGGRRQ 
ILLTRHHQR VCIVLHATL GTKPPLHAA CSPDGEDGV 
CLAQITIPS SWWPPLPSP DKDGRPGKI TKTPPRLVQ 
VAYSVMEPR PEDAEGCHP KMQIQPSTV LGSVPLVPP 
KGAYKELKL TDAITMLLP HPPLFPKSR MHVPVFIDK 
EKAKMLTAI VISSVYVDG SEARGSVNG SVLVKYGTY 
IGLARFTVW MPEFPLDLQ VTDTRLSQL KSWRVPDYH 
PSGVKSRRK KRSYASNWG GSNPPDDLG NVVEKPICR 
LRYQQSPVD VYAHFMAVD HDSGRISYL INRRTWLRV 
TDLVLPLLR VSDPRIASL HGRILQGRS MGRTEVQVL 
SPITGRVIG AKEIRVGND KVGLSRLLV QVVSGLQLN 
ISPDSSIEN GYIAETSVT RKLTAQYQE GLLDIELEF 
SDGTRTPLR DIADSDYHL VVESLDPEV VAFAPMVAS 
HHPRVIAVG EGNGDLLQV TLLLAEECR TSNRPKMKG 
AGPLATASA SVTVDFTSS DLQHRPDIL QNDGGSYGG 
SKREFQDLQ DILKGTPLK DDDSQEPNV QARQYQGGK 
GVLRQHSPQ HMTPLEISM YILLGTFCC AIVVFVVSC 
VVYASKFKA EEGAIAPGE RAAPVAPGS LVVHRKPRE 
TTTNAHDWV WLGRATLDS SNRNSSVAN TNGNEMRIT 
ANPLNLNYC DPDDCLATS FSNPSHIEL PSSSQADIN 
SNRPIDSNT YCKSKSGGS NNEDLQVWN KPTPPPPLP 
PHGVPLQPK PPQPVEDYR PPVPPHRNI GVTSEATPP 
RKHHHHHHH RNSKHQDKH HHDYGWAVN GWSKDQEEE 
DEDLVMTAS QEPMRSVFE FDDEPPKEE EKPEEVKLR 
SKPPKPDEE EVQFVQYPK SPNANGNGE LVPV
    CDS sequence
    • >TC031844 RA
      atgccggca ccggaacta aacggtcct tcctccacc 
aacggctct cctctctcg accgatcgc ttcaccgtg 
ttccaaagc tcgcagcct atctcagtc cgcgccacg 
tacggccct ttcagcacg aaacagacc gtaccggcg 
cgctacgtc gttccggac cctctgccg ctcaacaca 
acaggccct gccatcgac ttgcaagac ctcgccacg 
caccacttg gacatgtcg gcccacatc gtccggaat 
gagatcccg cgggactcc cccgtgctc cgggtgctc 
ttccacacc ggaacagat ccgggcggc cgcagacaa 
attctgtta acacgccac caccagcgc gtgtgtatc 
gtgctccac gccactctg ggcactaag ccgcccctc 
cacgctgcc tgcagcccc gacggagag gacggcgtg 
tgtttggcg caaattacg ataccgtct tcgtggtgg 
cccccgttg ccgagtccg gataaggac ggaaggccg 
ggcaaaatc accaaaacg ccgccaagg cttgtccaa 
gtggcgtat tcagtgatg gaaccgagg cctgaagat 
gcggaaggt tgccatccc aaaatgcaa atacagccg 
tcgacggtg ttaggcagt gtgccgctt gttccgcca 
aaaggtgcc tacaaggag ctcaaactg acagatgct 
ataacgatg ttactgccg catccgccg ttgtttccc 
aagtccagg atgcacgta ccggttttt atcgacaag 
gagaaggcg aaaatgcta acagcgatt gtcataagc 
tctgtttac gtggacgga agtgaggcc cgcggttcc 
gttaacggt tcagttctc gtaaaatac ggcacttat 
attggcctc gcccgcttc actgtctgg atgcccgaa 
ttccccttg gacctccaa gtgaccgac acgaggctg 
tctcagctc aagtcgtgg cgcgtcccg gattaccat 
cccagcggt gttaaatcc cgccggaag aagcgatcc 
tatgccagt aattgggga ggctctaat ccccccgat 
gacttgggg aatgtggta gaaaagccg atttgccgg 
ctgaggtat cagcagagc ccagtggac gtttatgcc 
cacttcatg gccgtggac cacgactcc ggaaggatc 
agttatctg atcaatcgg aggacgtgg ttaagggtc 
acggacttg gtcttgccc ttgttaagg gtgagcgac 
ccccgcatt gcaagcctg cacgggagg atactccaa 
gggcgcagc atgggccgg accgaggtg caggttctg 
agccccatc acgggacgt gtcattgga gccaaggaa 
atcagagtc gggaatgac aaagtggga ttatcgcgg 
cttctggtc caagttgtc tcaggatta caactaaat 
atctcgccc gatagttcg atcgaaaac ggctacata 
gccgagaca agtgttaca aggaaatta acagctcaa 
taccaggag ggcctgctt gacatcgag ctggaattt 
tccgatggg actagaacc ccactccgg gatattgca 
gactcggat tatcacctc gtggtcgag agtctggac 
ccagaggtg gtggcgttt gcccccatg gtggcgtcc 
caccaccca cgcgtgata gcagtgggt gaaggcaac 
ggagacttg ctacaagtt actctattg ttagctgaa 
gaatgtaga actagcaat aggccaaaa atgaagggc 
gctgggcct ctggccact gcctcagct tcggtcact 
gttgacttt acttcaagt gacttacaa cacaggccc 
gatatttta cagaatgac ggggggtcg tatgggggc 
tccaagagg gaatttcag gatcttcag gacatttta 
aaaggcact cccttgaaa gacgacgac tcgcaggag 
cccaacgtg caggcccgc caataccaa gggggcaaa 
ggggtgctg cggcagcac tccccccag cacatgacg 
ccgctcgaa atcagcatg tacatcctc ctgggcacg 
ttctgctgc gccatcgtt gttttcgtg gtatcgtgc 
gtggtctac gcgtccaag ttcaaggcg gaagaaggc 
gcgattgct ccgggtgag agggccgct ccggtggcc 
cccggaagc ctggtagtg catcgcaaa ccccgagaa 
accacgaca aacgcccac gattgggtc tggctaggt 
cgtgccact ttggacagt tcgaatcga aattcctca 
gttgcgaac acaaacgga aacgaaatg agaattaca 
gcgaatccg ctcaatttg aattattgc gatccggat 
gattgtcta gcgacgagt tttagtaat cctagtcat 
atcgagctg ccgagctcg tcgcaggcc gatattaat 
tccaatagg ccgatcgat agcaacacc tattgcaag 
agcaagagt ggaggcagc aacaatgag gatctacaa 
gtgtggaac aaaccaacg ccgcctcca ccgctcccc 
ccgcacggc gtgcccctc cagccgaag cccccgcag 
ccggtggag gactaccgg ccccctgtt ccgccccat 
cgcaacatt ggagtgact tccgaagcc acccctccc 
cgcaagcat caccaccac caccatcac aggaacagc 
aaacaccaa gacaagcac caccacgac tacggatgg 
gccgtcaac ggctggtcc aaggaccag gaggaggaa 
gacgaagac ttagtaatg acagcgagc caggaaccc 
atgcgcagc gtgttcgag ttcgacgac gagcctccg 
aaggaggaa gagaagccg gaagaggtg aagctgcgg 
agcaagccc ccgaagccc gacgaggag gaggtccag 
ttcgtgcag tacccgaag tcgccgaac gcgaacgga 
aacggtgag ctggtccct gtttga
    mRNA sequence
    • >TC031844 RA
      ttccagctt actcagaat gcgtgaacg tacatttcg 
aaaacaaag acggaggct tcttcctga aacaatctc 
ctcgtcagc atttcatgc cggcaccgg aactaaacg 
gtccttcct ccaccaacg gctctcctc tctcgaccg 
atcgcttca ccgtgttcc aaagctcgc agcctatct 
cagtccgcg ccacgtacg gccctttca gcacgaaac 
agaccgtac cggcgcgct acgtcgttc cggaccctc 
tgccgctca acacaacag gccctgcca tcgacttgc 
aagacctcg ccacgcacc acttggaca tgtcggccc 
acatcgtcc ggaatgaga tcccgcggg actcccccg 
tgctccggg tgctcttcc acaccggaa cagatccgg 
gcggccgca gacaaattc tgttaacac gccaccacc 
agcgcgtgt gtatcgtgc tccacgcca ctctgggca 
ctaagccgc ccctccacg ctgcctgca gccccgacg 
gagaggacg gcgtgtgtt tggcgcaaa ttacgatac 
cgtcttcgt ggtggcccc cgttgccga gtccggata 
aggacggaa ggccgggca aaatcacca aaacgccgc 
caaggcttg tccaagtgg cgtattcag tgatggaac 
cgaggcctg aagatgcgg aaggttgcc atcccaaaa 
tgcaaatac agccgtcga cggtgttag gcagtgtgc 
cgcttgttc cgccaaaag gtgcctaca aggagctca 
aactgacag atgctataa cgatgttac tgccgcatc 
cgccgttgt ttcccaagt ccaggatgc acgtaccgg 
tttttatcg acaaggaga aggcgaaaa tgctaacag 
cgattgtca taagctctg tttacgtgg acggaagtg 
aggcccgcg gttccgtta acggttcag ttctcgtaa 
aatacggca cttatattg gcctcgccc gcttcactg 
tctggatgc ccgaattcc ccttggacc tccaagtga 
ccgacacga ggctgtctc agctcaagt cgtggcgcg 
tcccggatt accatccca gcggtgtta aatcccgcc 
ggaagaagc gatcctatg ccagtaatt ggggaggct 
ctaatcccc ccgatgact tggggaatg tggtagaaa 
agccgattt gccggctga ggtatcagc agagcccag 
tggacgttt atgcccact tcatggccg tggaccacg 
actccggaa ggatcagtt atctgatca atcggagga 
cgtggttaa gggtcacgg acttggtct tgcccttgt 
taagggtga gcgaccccc gcattgcaa gcctgcacg 
ggaggatac tccaagggc gcagcatgg gccggaccg 
aggtgcagg ttctgagcc ccatcacgg gacgtgtca 
ttggagcca aggaaatca gagtcggga atgacaaag 
tgggattat cgcggcttc tggtccaag ttgtctcag 
gattacaac taaatatct cgcccgata gttcgatcg 
aaaacggct acatagccg agacaagtg ttacaagga 
aattaacag ctcaatacc aggagggcc tgcttgaca 
tcgagctgg aattttccg atgggacta gaaccccac 
tccgggata ttgcagact cggattatc acctcgtgg 
tcgagagtc tggacccag aggtggtgg cgtttgccc 
ccatggtgg cgtcccacc acccacgcg tgatagcag 
tgggtgaag gcaacggag acttgctac aagttactc 
tattgttag ctgaagaat gtagaacta gcaataggc 
caaaaatga agggcgctg ggcctctgg ccactgcct 
cagcttcgg tcactgttg actttactt caagtgact 
tacaacaca ggcccgata ttttacaga atgacgggg 
ggtcgtatg ggggctcca agagggaat ttcaggatc 
ttcaggaca ttttaaaag gcactccct tgaaagacg 
acgactcgc aggagccca acgtgcagg cccgccaat 
accaagggg gcaaagggg tgctgcggc agcactccc 
cccagcaca tgacgccgc tcgaaatca gcatgtaca 
tcctcctgg gcacgttct gctgcgcca tcgttgttt 
tcgtggtat cgtgcgtgg tctacgcgt ccaagttca 
aggcggaag aaggcgcga ttgctccgg gtgagaggg 
ccgctccgg tggcccccg gaagcctgg tagtgcatc 
gcaaacccc gagaaacca cgacaaacg cccacgatt 
gggtctggc taggtcgtg ccactttgg acagttcga 
atcgaaatt cctcagttg cgaacacaa acggaaacg 
aaatgagaa ttacagcga atccgctca atttgaatt 
attgcgatc cggatgatt gtctagcga cgagtttta 
gtaatccta gtcatatcg agctgccga gctcgtcgc 
aggccgata ttaattcca ataggccga tcgatagca 
acacctatt gcaagagca agagtggag gcagcaaca 
atgaggatc tacaagtgt ggaacaaac caacgccgc 
ctccaccgc tccccccgc acggcgtgc ccctccagc 
cgaagcccc cgcagccgg tggaggact accggcccc 
ctgttccgc cccatcgca acattggag tgacttccg 
aagccaccc ctccccgca agcatcacc accaccacc 
atcacagga acagcaaac accaagaca agcaccacc 
acgactacg gatgggccg tcaacggct ggtccaagg 
accaggagg aggaagacg aagacttag taatgacag 
cgagccagg aacccatgc gcagcgtgt tcgagttcg 
acgacgagc ctccgaagg aggaagaga agccggaag 
aggtgaagc tgcggagca agcccccga agcccgacg 
aggaggagg tccagttcg tgcagtacc cgaagtcgc 
cgaacgcga acggaaacg gtgagctgg tccctgttt 
gaaggcaaa ccaactgag cgcggtttc aggcgcagc 
agcgcggag gtgaagcgc gccaccatt gtggggaac 
cccatgttc agcagcgcg gacgcgccc cttccggcg 
acctgccgg gnnnnnnnn nnnnnn
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY