General information | |||
TC031844
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451510.1: 20716..60613 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031844 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02908 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06734 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031844 RA
MPAPELNGP SSTNGSPLS TDRFTVFQS SQPISVRAT YGPFSTKQT VPARYVVPD PLPLNTTGP AIDLQDLAT HHLDMSAHI VRNEIPRDS PVLRVLFHT GTDPGGRRQ ILLTRHHQR VCIVLHATL GTKPPLHAA CSPDGEDGV CLAQITIPS SWWPPLPSP DKDGRPGKI TKTPPRLVQ VAYSVMEPR PEDAEGCHP KMQIQPSTV LGSVPLVPP KGAYKELKL TDAITMLLP HPPLFPKSR MHVPVFIDK EKAKMLTAI VISSVYVDG SEARGSVNG SVLVKYGTY IGLARFTVW MPEFPLDLQ VTDTRLSQL KSWRVPDYH PSGVKSRRK KRSYASNWG GSNPPDDLG NVVEKPICR LRYQQSPVD VYAHFMAVD HDSGRISYL INRRTWLRV TDLVLPLLR VSDPRIASL HGRILQGRS MGRTEVQVL SPITGRVIG AKEIRVGND KVGLSRLLV QVVSGLQLN ISPDSSIEN GYIAETSVT RKLTAQYQE GLLDIELEF SDGTRTPLR DIADSDYHL VVESLDPEV VAFAPMVAS HHPRVIAVG EGNGDLLQV TLLLAEECR TSNRPKMKG AGPLATASA SVTVDFTSS DLQHRPDIL QNDGGSYGG SKREFQDLQ DILKGTPLK DDDSQEPNV QARQYQGGK GVLRQHSPQ HMTPLEISM YILLGTFCC AIVVFVVSC VVYASKFKA EEGAIAPGE RAAPVAPGS LVVHRKPRE TTTNAHDWV WLGRATLDS SNRNSSVAN TNGNEMRIT ANPLNLNYC DPDDCLATS FSNPSHIEL PSSSQADIN SNRPIDSNT YCKSKSGGS NNEDLQVWN KPTPPPPLP PHGVPLQPK PPQPVEDYR PPVPPHRNI GVTSEATPP RKHHHHHHH RNSKHQDKH HHDYGWAVN GWSKDQEEE DEDLVMTAS QEPMRSVFE FDDEPPKEE EKPEEVKLR SKPPKPDEE EVQFVQYPK SPNANGNGE LVPV
- >TC031844 RA
atgccggca ccggaacta aacggtcct tcctccacc aacggctct cctctctcg accgatcgc ttcaccgtg ttccaaagc tcgcagcct atctcagtc cgcgccacg tacggccct ttcagcacg aaacagacc gtaccggcg cgctacgtc gttccggac cctctgccg ctcaacaca acaggccct gccatcgac ttgcaagac ctcgccacg caccacttg gacatgtcg gcccacatc gtccggaat gagatcccg cgggactcc cccgtgctc cgggtgctc ttccacacc ggaacagat ccgggcggc cgcagacaa attctgtta acacgccac caccagcgc gtgtgtatc gtgctccac gccactctg ggcactaag ccgcccctc cacgctgcc tgcagcccc gacggagag gacggcgtg tgtttggcg caaattacg ataccgtct tcgtggtgg cccccgttg ccgagtccg gataaggac ggaaggccg ggcaaaatc accaaaacg ccgccaagg cttgtccaa gtggcgtat tcagtgatg gaaccgagg cctgaagat gcggaaggt tgccatccc aaaatgcaa atacagccg tcgacggtg ttaggcagt gtgccgctt gttccgcca aaaggtgcc tacaaggag ctcaaactg acagatgct ataacgatg ttactgccg catccgccg ttgtttccc aagtccagg atgcacgta ccggttttt atcgacaag gagaaggcg aaaatgcta acagcgatt gtcataagc tctgtttac gtggacgga agtgaggcc cgcggttcc gttaacggt tcagttctc gtaaaatac ggcacttat attggcctc gcccgcttc actgtctgg atgcccgaa ttccccttg gacctccaa gtgaccgac acgaggctg tctcagctc aagtcgtgg cgcgtcccg gattaccat cccagcggt gttaaatcc cgccggaag aagcgatcc tatgccagt aattgggga ggctctaat ccccccgat gacttgggg aatgtggta gaaaagccg atttgccgg ctgaggtat cagcagagc ccagtggac gtttatgcc cacttcatg gccgtggac cacgactcc ggaaggatc agttatctg atcaatcgg aggacgtgg ttaagggtc acggacttg gtcttgccc ttgttaagg gtgagcgac ccccgcatt gcaagcctg cacgggagg atactccaa gggcgcagc atgggccgg accgaggtg caggttctg agccccatc acgggacgt gtcattgga gccaaggaa atcagagtc gggaatgac aaagtggga ttatcgcgg cttctggtc caagttgtc tcaggatta caactaaat atctcgccc gatagttcg atcgaaaac ggctacata gccgagaca agtgttaca aggaaatta acagctcaa taccaggag ggcctgctt gacatcgag ctggaattt tccgatggg actagaacc ccactccgg gatattgca gactcggat tatcacctc gtggtcgag agtctggac ccagaggtg gtggcgttt gcccccatg gtggcgtcc caccaccca cgcgtgata gcagtgggt gaaggcaac ggagacttg ctacaagtt actctattg ttagctgaa gaatgtaga actagcaat aggccaaaa atgaagggc gctgggcct ctggccact gcctcagct tcggtcact gttgacttt acttcaagt gacttacaa cacaggccc gatatttta cagaatgac ggggggtcg tatgggggc tccaagagg gaatttcag gatcttcag gacatttta aaaggcact cccttgaaa gacgacgac tcgcaggag cccaacgtg caggcccgc caataccaa gggggcaaa ggggtgctg cggcagcac tccccccag cacatgacg ccgctcgaa atcagcatg tacatcctc ctgggcacg ttctgctgc gccatcgtt gttttcgtg gtatcgtgc gtggtctac gcgtccaag ttcaaggcg gaagaaggc gcgattgct ccgggtgag agggccgct ccggtggcc cccggaagc ctggtagtg catcgcaaa ccccgagaa accacgaca aacgcccac gattgggtc tggctaggt cgtgccact ttggacagt tcgaatcga aattcctca gttgcgaac acaaacgga aacgaaatg agaattaca gcgaatccg ctcaatttg aattattgc gatccggat gattgtcta gcgacgagt tttagtaat cctagtcat atcgagctg ccgagctcg tcgcaggcc gatattaat tccaatagg ccgatcgat agcaacacc tattgcaag agcaagagt ggaggcagc aacaatgag gatctacaa gtgtggaac aaaccaacg ccgcctcca ccgctcccc ccgcacggc gtgcccctc cagccgaag cccccgcag ccggtggag gactaccgg ccccctgtt ccgccccat cgcaacatt ggagtgact tccgaagcc acccctccc cgcaagcat caccaccac caccatcac aggaacagc aaacaccaa gacaagcac caccacgac tacggatgg gccgtcaac ggctggtcc aaggaccag gaggaggaa gacgaagac ttagtaatg acagcgagc caggaaccc atgcgcagc gtgttcgag ttcgacgac gagcctccg aaggaggaa gagaagccg gaagaggtg aagctgcgg agcaagccc ccgaagccc gacgaggag gaggtccag ttcgtgcag tacccgaag tcgccgaac gcgaacgga aacggtgag ctggtccct gtttga
- >TC031844 RA
ttccagctt actcagaat gcgtgaacg tacatttcg aaaacaaag acggaggct tcttcctga aacaatctc ctcgtcagc atttcatgc cggcaccgg aactaaacg gtccttcct ccaccaacg gctctcctc tctcgaccg atcgcttca ccgtgttcc aaagctcgc agcctatct cagtccgcg ccacgtacg gccctttca gcacgaaac agaccgtac cggcgcgct acgtcgttc cggaccctc tgccgctca acacaacag gccctgcca tcgacttgc aagacctcg ccacgcacc acttggaca tgtcggccc acatcgtcc ggaatgaga tcccgcggg actcccccg tgctccggg tgctcttcc acaccggaa cagatccgg gcggccgca gacaaattc tgttaacac gccaccacc agcgcgtgt gtatcgtgc tccacgcca ctctgggca ctaagccgc ccctccacg ctgcctgca gccccgacg gagaggacg gcgtgtgtt tggcgcaaa ttacgatac cgtcttcgt ggtggcccc cgttgccga gtccggata aggacggaa ggccgggca aaatcacca aaacgccgc caaggcttg tccaagtgg cgtattcag tgatggaac cgaggcctg aagatgcgg aaggttgcc atcccaaaa tgcaaatac agccgtcga cggtgttag gcagtgtgc cgcttgttc cgccaaaag gtgcctaca aggagctca aactgacag atgctataa cgatgttac tgccgcatc cgccgttgt ttcccaagt ccaggatgc acgtaccgg tttttatcg acaaggaga aggcgaaaa tgctaacag cgattgtca taagctctg tttacgtgg acggaagtg aggcccgcg gttccgtta acggttcag ttctcgtaa aatacggca cttatattg gcctcgccc gcttcactg tctggatgc ccgaattcc ccttggacc tccaagtga ccgacacga ggctgtctc agctcaagt cgtggcgcg tcccggatt accatccca gcggtgtta aatcccgcc ggaagaagc gatcctatg ccagtaatt ggggaggct ctaatcccc ccgatgact tggggaatg tggtagaaa agccgattt gccggctga ggtatcagc agagcccag tggacgttt atgcccact tcatggccg tggaccacg actccggaa ggatcagtt atctgatca atcggagga cgtggttaa gggtcacgg acttggtct tgcccttgt taagggtga gcgaccccc gcattgcaa gcctgcacg ggaggatac tccaagggc gcagcatgg gccggaccg aggtgcagg ttctgagcc ccatcacgg gacgtgtca ttggagcca aggaaatca gagtcggga atgacaaag tgggattat cgcggcttc tggtccaag ttgtctcag gattacaac taaatatct cgcccgata gttcgatcg aaaacggct acatagccg agacaagtg ttacaagga aattaacag ctcaatacc aggagggcc tgcttgaca tcgagctgg aattttccg atgggacta gaaccccac tccgggata ttgcagact cggattatc acctcgtgg tcgagagtc tggacccag aggtggtgg cgtttgccc ccatggtgg cgtcccacc acccacgcg tgatagcag tgggtgaag gcaacggag acttgctac aagttactc tattgttag ctgaagaat gtagaacta gcaataggc caaaaatga agggcgctg ggcctctgg ccactgcct cagcttcgg tcactgttg actttactt caagtgact tacaacaca ggcccgata ttttacaga atgacgggg ggtcgtatg ggggctcca agagggaat ttcaggatc ttcaggaca ttttaaaag gcactccct tgaaagacg acgactcgc aggagccca acgtgcagg cccgccaat accaagggg gcaaagggg tgctgcggc agcactccc cccagcaca tgacgccgc tcgaaatca gcatgtaca tcctcctgg gcacgttct gctgcgcca tcgttgttt tcgtggtat cgtgcgtgg tctacgcgt ccaagttca aggcggaag aaggcgcga ttgctccgg gtgagaggg ccgctccgg tggcccccg gaagcctgg tagtgcatc gcaaacccc gagaaacca cgacaaacg cccacgatt gggtctggc taggtcgtg ccactttgg acagttcga atcgaaatt cctcagttg cgaacacaa acggaaacg aaatgagaa ttacagcga atccgctca atttgaatt attgcgatc cggatgatt gtctagcga cgagtttta gtaatccta gtcatatcg agctgccga gctcgtcgc aggccgata ttaattcca ataggccga tcgatagca acacctatt gcaagagca agagtggag gcagcaaca atgaggatc tacaagtgt ggaacaaac caacgccgc ctccaccgc tccccccgc acggcgtgc ccctccagc cgaagcccc cgcagccgg tggaggact accggcccc ctgttccgc cccatcgca acattggag tgacttccg aagccaccc ctccccgca agcatcacc accaccacc atcacagga acagcaaac accaagaca agcaccacc acgactacg gatgggccg tcaacggct ggtccaagg accaggagg aggaagacg aagacttag taatgacag cgagccagg aacccatgc gcagcgtgt tcgagttcg acgacgagc ctccgaagg aggaagaga agccggaag aggtgaagc tgcggagca agcccccga agcccgacg aggaggagg tccagttcg tgcagtacc cgaagtcgc cgaacgcga acggaaacg gtgagctgg tccctgttt gaaggcaaa ccaactgag cgcggtttc aggcgcagc agcgcggag gtgaagcgc gccaccatt gtggggaac cccatgttc agcagcgcg gacgcgccc cttccggcg acctgccgg gnnnnnnnn nnnnnn
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
