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Gene information
General information
TC006976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451036.1: 244429..248076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006976
      iBeetle screen iB_06729

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006976 RA
        MERLATENL FLLEFLVDD VKMEGKCDC DTPPGEHCV 
        SFQFLDNDA LDVCEADFS PGRKYGKED NTKSGKSCL 
        FSLTPEQVQ KVSEVFDVT VTVFKKMQP GWLPDKIAI 
        GSALISIAN LFVELIQSV EVQADQTPS AKTMKDTFV 
        LMDSTGSKV GKISIYIRM SCFGKLIVT QFQMNLEDK 
        SVLFKDKGG QSLYRYKKA SKKNEPEEI FQNPLNTPW 
        NSQCPTIPC DQPDFNTMG FGGQINQMY PPQPQPNLN 
        CPMTPCFPD GMFPQDMMM GGGGGNIYG MPTSPQSDM 
        FSPMGQSML PCSECNIMM QQQFSAPQQ LQQQQQQQI 
        PCMMCPPEG MTSQMLTPC EECGTLLAP QFKAEVGGS 
        PSGPASDPP GNYQEIGAS MGGNALTIR VHKDKNRIE 
        QVDSVVGSE GSCCCSPGN IGVKKQKQT VSSQSNAGS 
        SPPQCFDVR PGIQQGDKN LPFSFKMSG AGASPNNNV 
        IINPPVCTK PDGTKFTEI TDPNKEMFV LRIGKKSEG 
        VQKKANLEL ELCTPRGPE LKPIPRKET RDSQYDPAD 
        CKGAGDKKK KGKKGKKDK KGKGKGKGK GKKGKKGKK 
        K
      CDS sequence
      • >TC006976 RA
        atggagaga cttgcaaca gaaaattta ttcctcctg 
        gaatttctc gtcgatgat gtgaaaatg gagggaaaa 
        tgtgactgc gacactccg ccaggcgag cattgcgtc 
        tcattccag tttctggat aatgatgct ttggacgtg 
        tgtgaggcg gatttttca ccaggaaga aaatatgga 
        aaagaagat aacacaaaa agtgggaaa tcttgtctg 
        ttttcgctc actccagag caagtgcag aaagtcagt 
        gaggtgttt gatgtcact gtgacggtg tttaagaaa 
        atgcagcct ggatggttg cctgataag attgcgata 
        ggaagtgct ttgatttcg attgcgaat ttgttcgtt 
        gagttgatt cagagtgtg gaggtgcaa gctgatcag 
        acaccttcg gcgaaaacg atgaaagac acctttgtt 
        ttgatggat tcgactggg agtaaagtt gggaagatt 
        agcatttat ataaggatg tcttgtttt ggaaaactg 
        attgtgaca cagtttcag atgaatctt gaagacaaa 
        tctgttctg ttcaaagac aaaggcggt caatcattg 
        taccgttac aaaaaagcg agtaaaaaa aacgaacct 
        gaagaaatc ttccaaaac cctctcaat accccgtgg 
        aactcccaa tgccccacc atcccctgt gaccaaccc 
        gactttaac acaatgggc tttggtggt caaatcaac 
        caaatgtac ccaccacaa ccacaacca aacttaaac 
        tgtcccatg acaccgtgt tttcccgat ggtatgttc 
        ccccaggat atgatgatg ggtggtggt ggtggtaat 
        atttatgga atgcccact tctccccaa tcggatatg 
        ttctcacca atgggtcaa tccatgtta ccatgcagt 
        gaatgtaat ataatgatg caacaacaa ttttccgcc 
        ccacaacaa ttacaacag caacaacaa caacaaatc 
        ccctgtatg atgtgtcct cccgaaggg atgacttca 
        caaatgttg actccttgt gaagagtgt ggtacactt 
        ttagcacca caatttaaa gctgaagtt gggggaagt 
        ccaagtgga ccagccagc gatccaccg gggaattac 
        caagaaatt ggtgctagt atgggtgga aatgcctta 
        actattagg gtacacaaa gataaaaac aggattgaa 
        caggtggat tctgtggtt ggtagcgag ggcagttgt 
        tgttgttct cctggcaat attggtgtg aaaaaacaa 
        aaacaaaca gtgtcaagt caaagtaat gcaggatct 
        tcgcctcct cagtgtttt gatgtgcgt ccaggaatt 
        caacaaggc gataaaaat ttaccattc tcatttaaa 
        atgtccgga gctggtgct tcccccaat aataatgtt 
        ataataaat ccaccggtt tgtacaaaa cctgatggt 
        acaaagttt accgaaatc acggatccg aataaagaa 
        atgtttgtg ttacggatt gggaaaaaa agtgaaggt 
        gtacagaag aaagctaat ttggaattg gagttgtgt 
        acgccaaga gggcctgaa ttaaagcca attccgaga 
        aaggaaacg agagattct caatatgat ccagctgat 
        tgtaaagga gcaggtgat aagaagaag aaggggaag 
        aaagggaag aaggataag aaagggaaa ggaaaaggg 
        aaaggcaaa ggaaaaaaa gggaagaaa gggaagaag 
        aagtga
      mRNA sequence
      • >TC006976 RA
        tccctaaaa aattttcct aaataaaaa attgcctag 
        caccccatt ttgttattt cccctgtga catttccca 
        tccaaaatt tgtgcgtaa aatgtcaaa attctcgaa 
        aaattctac gtaaaaatg gagagactt gcaacagaa 
        aatttattc ctcctggaa tttctcgtc gatgatgtg 
        aaaatggag ggaaaatgt gactgcgac actccgcca 
        ggcgagcat tgcgtctca ttccagttt ctggataat 
        gatgctttg gacgtgtgt gaggcggat ttttcacca 
        ggaagaaaa tatggaaaa gaagataac acaaaaagt 
        gggaaatct tgtctgttt tcgctcact ccagagcaa 
        gtgcagaaa gtcagtgag gtgtttgat gtcactgtg 
        acggtgttt aagaaaatg cagcctgga tggttgcct 
        gataagatt gcgatagga agtgctttg atttcgatt 
        gcgaatttg ttcgttgag ttgattcag agtgtggag 
        gtgcaagct gatcagaca ccttcggcg aaaacgatg 
        aaagacacc tttgttttg atggattcg actgggagt 
        aaagttggg aagattagc atttatata aggatgtct 
        tgttttgga aaactgatt gtgacacag tttcagatg 
        aatcttgaa gacaaatct gttctgttc aaagacaaa 
        ggcggtcaa tcattgtac cgttacaaa aaagcgagt 
        aaaaaaaac gaacctgaa gaaatcttc caaaaccct 
        ctcaatacc ccgtggaac tcccaatgc cccaccatc 
        ccctgtgac caacccgac tttaacaca atgggcttt 
        ggtggtcaa atcaaccaa atgtaccca ccacaacca 
        caaccaaac ttaaactgt cccatgaca ccgtgtttt 
        cccgatggt atgttcccc caggatatg atgatgggt 
        ggtggtggt ggtaatatt tatggaatg cccacttct 
        ccccaatcg gatatgttc tcaccaatg ggtcaatcc 
        atgttacca tgcagtgaa tgtaatata atgatgcaa 
        caacaattt tccgcccca caacaatta caacagcaa 
        caacaacaa caaatcccc tgtatgatg tgtcctccc 
        gaagggatg acttcacaa atgttgact ccttgtgaa 
        gagtgtggt acactttta gcaccacaa tttaaagct 
        gaagttggg ggaagtcca agtggacca gccagcgat 
        ccaccgggg aattaccaa gaaattggt gctagtatg 
        ggtggaaat gccttaact attagggta cacaaagat 
        aaaaacagg attgaacag gtggattct gtggttggt 
        agcgagggc agttgttgt tgttctcct ggcaatatt 
        ggtgtgaaa aaacaaaaa caaacagtg tcaagtcaa 
        agtaatgca ggatcttcg cctcctcag tgttttgat 
        gtgcgtcca ggaattcaa caaggcgat aaaaattta 
        ccattctca tttaaaatg tccggagct ggtgcttcc 
        cccaataat aatgttata ataaatcca ccggtttgt 
        acaaaacct gatggtaca aagtttacc gaaatcacg 
        gatccgaat aaagaaatg tttgtgtta cggattggg 
        aaaaaaagt gaaggtgta cagaagaaa gctaatttg 
        gaattggag ttgtgtacg ccaagaggg cctgaatta 
        aagccaatt ccgagaaag gaaacgaga gattctcaa 
        tatgatcca gctgattgt aaaggagca ggtgataag 
        aagaagaag gggaagaaa gggaagaag gataagaaa 
        gggaaagga aaagggaaa ggcaaagga aaaaaaggg 
        aagaaaggg aagaagaag tgattgtgt gtgttgtgt 
        ttgtcattt tatgtttca tattgtttg tgtcaataa 
        attttggat tgtgtc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY