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Gene information
General information
TC006978 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451036.1: 203143..221546
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006978
    iBeetle screen iB_06728

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006978 RA
      MRPQIIAIF ALVLATQCD SGNNFRNGY QLDYREIAR 
      QNKITEQIL QNYLEKRLF PNNHRPLKA PTVWDILPR 
      ARRKPNNNI QDVLNTTDE TLENGESER VTFYNDDTK 
      DEDDFEDCP NCIDDSLPS ANRWTMPLL KLGEKRYYL 
      GIFFKANYF RATQYCRFH GMHLASITS QEENDKLEK 
      YIKDFGLGH EHFWTSGTD LAEEGHFFW MSTGRPITF 
      TNWNAGEPN NFQYENGEE ENCLELWNR DGKGLKWND 
      SPCSFETYF VCEVQH
    • >TC006978 RB
      MRPQIIAIF ALVLATQCD SGNNFRNDC PNCIDDSLP 
      SANRWTMPL LKLGEKRYY LGIFFKANY FRATQYCRF 
      HGMHLASIT SQEENDKLE KYIKDFGLG HEHFWTSGT 
      DLAEEGHFF WMSTGRPIT FTNWNAGEP NNFQYENGE 
      EENCLELWN RDGKGLKWN DSPCSFETY FVCEVQH
    • >TC006978 RC
      MRPQIIAIF ALVLATQCD SGNNFRNGY QLDYREIAR 
      QNKITEQIL QNYLEKRLF PNNHRPLKA PTVWDILPR 
      ARRKPNNNI QDVLNTTDE TLENGESER VTFYNDDTK 
      DEDDFEGMS CLSIEKHIC NLARSGQAP AAAAADLSF 
      SFTYYLVGR MFRCLLLSA FVLGGTFAA AGPSVRSGA 
      QTTDCPNCI DDSLPSANR WTMPLLKLG EKRYYLGIF 
      FKANYFRAT QYCRFHGMH LASITSQEE NDKLEKYIK 
      DFGLGHEHF WTSGTDLAE EGHFFWMST GRPITFTNW 
      NAGEPNNFQ YENGEEENC LELWNRDGK GLKWNDSPC 
      SFETYFVCE VQH
    CDS sequence
    • >TC006978 RA
      atgcgaccg caaataatc gcgattttc gctttggtg 
      cttgcgaca cagtgcgac tccggaaac aacttcaga 
      aatgggtat caattggat tatcgggag atagcaaga 
      cagaacaaa ataaccgaa caaatcttg caaaactac 
      ttggaaaaa cgcttgttt ccgaataat catcgtcct 
      ttgaaagcg cccaccgtt tgggatatt ttaccaaga 
      gcgaggagg aaacccaat aataatata caggatgtt 
      ctcaacact actgatgaa accctagaa aatggggaa 
      tcggaacgt gttactttt tacaacgac gacaccaaa 
      gatgaggac gattttgaa gattgtccc aattgcatc 
      gatgattcg ttgccttcg gccaatcgg tggacgatg 
      cctttgctt aaactcggg gagaagagg tactatcta 
      ggaattttc tttaaggca aactacttt cgagccact 
      cagtattgc agatttcat ggaatgcat ctcgccagt 
      ataacatca caggaagaa aatgacaag ttagagaaa 
      tatatcaaa gattttggt ctgggtcat gagcatttc 
      tggacttct ggcaccgat ttggccgaa gaaggccac 
      tttttctgg atgtcaacc ggtcgtccc atcaccttc 
      actaactgg aacgctggt gaacccaac aatttccag 
      tacgaaaac ggagaagag gaaaactgt ctggaattg 
      tggaatcga gacggcaaa ggcctgaaa tggaatgac 
      tctccatgt tcgttcgaa acgtatttc gtgtgtgaa 
      gtgcaacat tga
    • >TC006978 RB
      atgcgaccg caaataatc gcgattttc gctttggtg 
      cttgcgaca cagtgcgac tccggaaac aacttcaga 
      aatgattgt cccaattgc atcgatgat tcgttgcct 
      tcggccaat cggtggacg atgcctttg cttaaactc 
      ggggagaag aggtactat ctaggaatt ttctttaag 
      gcaaactac tttcgagcc actcagtat tgcagattt 
      catggaatg catctcgcc agtataaca tcacaggaa 
      gaaaatgac aagttagag aaatatatc aaagatttt 
      ggtctgggt catgagcat ttctggact tctggcacc 
      gatttggcc gaagaaggc cactttttc tggatgtca 
      accggtcgt cccatcacc ttcactaac tggaacgct 
      ggtgaaccc aacaatttc cagtacgaa aacggagaa 
      gaggaaaac tgtctggaa ttgtggaat cgagacggc 
      aaaggcctg aaatggaat gactctcca tgttcgttc 
      gaaacgtat ttcgtgtgt gaagtgcaa cattga
    • >TC006978 RC
      atgcgaccg caaataatc gcgattttc gctttggtg 
      cttgcgaca cagtgcgac tccggaaac aacttcaga 
      aatgggtat caattggat tatcgggag atagcaaga 
      cagaacaaa ataaccgaa caaatcttg caaaactac 
      ttggaaaaa cgcttgttt ccgaataat catcgtcct 
      ttgaaagcg cccaccgtt tgggatatt ttaccaaga 
      gcgaggagg aaacccaat aataatata caggatgtt 
      ctcaacact actgatgaa accctagaa aatggggaa 
      tcggaacgt gttactttt tacaacgac gacaccaaa 
      gatgaggac gattttgaa ggcatgtcg tgtttatca 
      attgaaaaa cacatttgt aatttagct cggtcgggc 
      caagcacca gcagcagca gcagcagac ttatctttt 
      tccttcact tattattta gttgggagg atgtttcgg 
      tgtctgctg ctgtcggct ttcgttttg ggcggcact 
      tttgcagcc gctggacct tcggtccga agcggcgcc 
      caaacgaca gattgtccc aattgcatc gatgattcg 
      ttgccttcg gccaatcgg tggacgatg cctttgctt 
      aaactcggg gagaagagg tactatcta ggaattttc 
      tttaaggca aactacttt cgagccact cagtattgc 
      agatttcat ggaatgcat ctcgccagt ataacatca 
      caggaagaa aatgacaag ttagagaaa tatatcaaa 
      gattttggt ctgggtcat gagcatttc tggacttct 
      ggcaccgat ttggccgaa gaaggccac tttttctgg 
      atgtcaacc ggtcgtccc atcaccttc actaactgg 
      aacgctggt gaacccaac aatttccag tacgaaaac 
      ggagaagag gaaaactgt ctggaattg tggaatcga 
      gacggcaaa ggcctgaaa tggaatgac tctccatgt 
      tcgttcgaa acgtatttc gtgtgtgaa gtgcaacat 
      tga
    mRNA sequence
    • >TC006978 RA
      gccataaac actttccca ctgtctgct ctacacagg 
      tgctctcga gttgtcgga cagtttgtc caacgtttc 
      gttcgtgtc gtgaccatc ttccgtaaa taaatgcga 
      caaaatgcg accgcaaat aatcgcgat tttcgcttt 
      ggtgcttgc gacacagtg cgactccgg aaacaactt 
      cagaaatgg gtatcaatt ggattatcg ggagatagc 
      aagacagaa caaaataac cgaacaaat cttgcaaaa 
      ctacttgga aaaacgctt gtttccgaa taatcatcg 
      tcctttgaa agcgcccac cgtttggga tattttacc 
      aagagcgag gaggaaacc caataataa tatacagga 
      tgttctcaa cactactga tgaaaccct agaaaatgg 
      ggaatcgga acgtgttac tttttacaa cgacgacac 
      caaagatga ggacgattt tgaagattg tcccaattg 
      catcgatga ttcgttgcc ttcggccaa tcggtggac 
      gatgccttt gcttaaact cggggagaa gaggtacta 
      tctaggaat tttctttaa ggcaaacta ctttcgagc 
      cactcagta ttgcagatt tcatggaat gcatctcgc 
      cagtataac atcacagga agaaaatga caagttaga 
      gaaatatat caaagattt tggtctggg tcatgagca 
      tttctggac ttctggcac cgatttggc cgaagaagg 
      ccacttttt ctggatgtc aaccggtcg tcccatcac 
      cttcactaa ctggaacgc tggtgaacc caacaattt 
      ccagtacga aaacggaga agaggaaaa ctgtctgga 
      attgtggaa tcgagacgg caaaggcct gaaatggaa 
      tgactctcc atgttcgtt cgaaacgta tttcgtgtg 
      tgaagtgca acattgaaa gtgattttt taatagtga 
      tttaaataa aatcaaagc ctgggacta tgagagaga 
      cagacgggc ttggactga agttgcaag tgcgactag 
      aagttagat atttaattt tttaccgat tgattttat 
      tagagttcg taacctaag tcataattt tgcaatgat 
      tgattgatt gttgtacaa agtcgaata aaaatatat 
      ttaataaaa acttgtgat tgat
    • >TC006978 RB
      gccataaac actttccca ctgtctgct ctacacagg 
      tgctctcga gttgtcgga cagtttgtc caacgtttc 
      gttcgtgtc gtgaccatc ttccgtaaa taaatgcga 
      caaaatgcg accgcaaat aatcgcgat tttcgcttt 
      ggtgcttgc gacacagtg cgactccgg aaacaactt 
      cagaaatga ttgtcccaa ttgcatcga tgattcgtt 
      gccttcggc caatcggtg gacgatgcc tttgcttaa 
      actcgggga gaagaggta ctatctagg aattttctt 
      taaggcaaa ctactttcg agccactca gtattgcag 
      atttcatgg aatgcatct cgccagtat aacatcaca 
      ggaagaaaa tgacaagtt agagaaata tatcaaaga 
      ttttggtct gggtcatga gcatttctg gacttctgg 
      caccgattt ggccgaaga aggccactt tttctggat 
      gtcaaccgg tcgtcccat caccttcac taactggaa 
      cgctggtga acccaacaa tttccagta cgaaaacgg 
      agaagagga aaactgtct ggaattgtg gaatcgaga 
      cggcaaagg cctgaaatg gaatgactc tccatgttc 
      gttcgaaac gtatttcgt gtgtgaagt gcaacattg 
      aaagtgatt ttttaatag tgatttaaa taaaatcaa 
      agcctggga ctatgagag agacagacg ggcttggac 
      tgaagttgc aagtgcgac tagaagtta gatatttaa 
      ttttttacc gattgattt tattagagt tcgtaacct 
      aagtcataa ttttgcaat gattgattg attgttgta 
      caaagtcga ataaaaata tatttaata aaaacttgt 
      gattgat
    • >TC006978 RC
      gccataaac actttccca ctgtctgct ctacacagg 
      tgctctcga gttgtcgga cagtttgtc caacgtttc 
      gttcgtgtc gtgaccatc ttccgtaaa taaatgcga 
      caaaatgcg accgcaaat aatcgcgat tttcgcttt 
      ggtgcttgc gacacagtg cgactccgg aaacaactt 
      cagaaatgg gtatcaatt ggattatcg ggagatagc 
      aagacagaa caaaataac cgaacaaat cttgcaaaa 
      ctacttgga aaaacgctt gtttccgaa taatcatcg 
      tcctttgaa agcgcccac cgtttggga tattttacc 
      aagagcgag gaggaaacc caataataa tatacagga 
      tgttctcaa cactactga tgaaaccct agaaaatgg 
      ggaatcgga acgtgttac tttttacaa cgacgacac 
      caaagatga ggacgattt tgaaggcat gtcgtgttt 
      atcaattga aaaacacat ttgtaattt agctcggtc 
      gggccaagc accagcagc agcagcagc agacttatc 
      tttttcctt cacttatta tttagttgg gaggatgtt 
      tcggtgtct gctgctgtc ggctttcgt tttgggcgg 
      cacttttgc agccgctgg accttcggt ccgaagcgg 
      cgcccaaac gacagattg tcccaattg catcgatga 
      ttcgttgcc ttcggccaa tcggtggac gatgccttt 
      gcttaaact cggggagaa gaggtacta tctaggaat 
      tttctttaa ggcaaacta ctttcgagc cactcagta 
      ttgcagatt tcatggaat gcatctcgc cagtataac 
      atcacagga agaaaatga caagttaga gaaatatat 
      caaagattt tggtctggg tcatgagca tttctggac 
      ttctggcac cgatttggc cgaagaagg ccacttttt 
      ctggatgtc aaccggtcg tcccatcac cttcactaa 
      ctggaacgc tggtgaacc caacaattt ccagtacga 
      aaacggaga agaggaaaa ctgtctgga attgtggaa 
      tcgagacgg caaaggcct gaaatggaa tgactctcc 
      atgttcgtt cgaaacgta tttcgtgtg tgaagtgca 
      acattgaaa gtgattttt taatagtga tttaaataa 
      aatcaaagc ctgggacta tgagagaga cagacgggc 
      ttggactga agttgcaag tgcgactag aagttagat 
      atttaattt tttaccgat tgattttat tagagttcg 
      taacctaag tcataattt tgcaatgat tgattgatt 
      gttgtacaa agtcgaata aaaatatat ttaataaaa 
      acttgtgat tgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY