Status
Please wait ...
Gene information
General information
TC006987 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451036.1: 164617..166446
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006987
    iBeetle screen iB_06724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006987 RA
      MMSTRNVWI ICGIIVTIA IYWPTRHAR RYDYIDLKD 
      VYKELQFTN KLSHLRNCN YKDVILNNS DVEISEPKN 
      YFTPEHGGV HKPNHCNPL IKVAVIVPY RNRSLQLET 
      FLNYMHNFL QKQYLFYRI FIVNQVDSL PFNRAKMLN 
      YGAKVAIDM DFPCLILHD VDLLPLNSG NIYGCVSKP 
      RHMSSSVDT FRFNLPYLT LFGGVVAIS SQHFRKING 
      LSNHFYGWG GEDDDFYKR LTINDLSPC RFTPVLSRY 
      TMLFHKKEK ASPNRYDNM EKSAVLHKS DGLSTLPSN 
      YSIKMEKLY TLILAS
    CDS sequence
    • >TC006987 RA
      atgatgtcg acaagaaac gtttggatt atttgtggt 
      attattgta acgattgca atttactgg cctacaaga 
      cacgcccga cgttacgat tacattgat ctcaaagat 
      gtgtacaaa gaattacaa tttacgaac aagttatca 
      catttgaga aactgcaat tacaaagat gtcattttg 
      aataacagt gatgtagaa attagtgaa cctaagaat 
      tattttaca cccgaacat ggtggtgtg cataaacca 
      aaccattgt aatcctcta ataaaagtc gctgtgata 
      gttccgtac cgaaacaga tcgttacag ttagaaacg 
      tttctgaat tacatgcac aactttcta cagaagcaa 
      tatttattt tacagaatt tttatcgtg aatcaagtc 
      gacagctta ccgtttaat cgagcgaaa atgctcaat 
      tatggtgca aaagttgca attgatatg gattttcct 
      tgccttatt ttacatgac gttgatctt ttacctcta 
      aatagtgga aatatttac ggttgtgtg agcaaacca 
      agacacatg tctagtagt gtagatact tttagattt 
      aatttaccg tatttaacg ttatttggg ggagttgtg 
      gcgatttca tcgcaacat tttcggaaa attaatggt 
      ttgtctaat catttttat ggctggggt ggggaagac 
      gacgatttt tacaagcga ctcactatc aatgattta 
      tcaccttgt cgttttacc cccgtttta agtaggtat 
      acaatgctc tttcataaa aaagagaaa gctagtccg 
      aacagatat gacaatatg gaaaaatcc gcagtttta 
      cacaaatcg gatggattg tcaacgcta ccttcaaat 
      tattctatc aaaatggaa aagttatac acattaatt 
      ttagcgtct taa
    mRNA sequence
    • >TC006987 RA
      taaaatttg aaagttaat tatcgagtt aattcagct 
      acaaaccgt gtaattacc cgacacact taaactgct 
      acagatgtg tcagattta ttaactcaa cttgtggca 
      atagatcag aaaaataat aatttggtt ggtcccatt 
      gttacatta aaattcaaa gtgatgaat taataaaag 
      tagataaaa tatgaataa tccgcataa gtgaaatta 
      tggggaaaa gggggaaac agtatgact aaattcttt 
      aaattgtaa tgaattgat tagtcagta gtcaggaaa 
      cctaacctc acttggcct agaagaagg cagaaacaa 
      gtcttttat ttttaattg gtgtaataa tgtgaatat 
      ttcagtggc agagcgaat aaaatgaaa ttctctcgt 
      tgcgcaaga cacaatact taatcccct tatcaggaa 
      aatgtattt ctcctagca tgatgtcga caagaaacg 
      tttggatta tttgtggta ttattgtaa cgattgcaa 
      tttactggc ctacaagac acgcccgac gttacgatt 
      acattgatc tcaaagatg tgtacaaag aattacaat 
      ttacgaaca agttatcac atttgagaa actgcaatt 
      acaaagatg tcattttga ataacagtg atgtagaaa 
      ttagtgaac ctaagaatt attttacac ccgaacatg 
      gtggtgtgc ataaaccaa accattgta atcctctaa 
      taaaagtcg ctgtgatag ttccgtacc gaaacagat 
      cgttacagt tagaaacgt ttctgaatt acatgcaca 
      actttctac agaagcaat atttatttt acagaattt 
      ttatcgtga atcaagtcg acagcttac cgtttaatc 
      gagcgaaaa tgctcaatt atggtgcaa aagttgcaa 
      ttgatatgg attttcctt gccttattt tacatgacg 
      ttgatcttt tacctctaa atagtggaa atatttacg 
      gttgtgtga gcaaaccaa gacacatgt ctagtagtg 
      tagatactt ttagattta atttaccgt atttaacgt 
      tatttgggg gagttgtgg cgatttcat cgcaacatt 
      ttcggaaaa ttaatggtt tgtctaatc atttttatg 
      gctggggtg gggaagacg acgattttt acaagcgac 
      tcactatca atgatttat caccttgtc gttttaccc 
      ccgttttaa gtaggtata caatgctct ttcataaaa 
      aagagaaag ctagtccga acagatatg acaatatgg 
      aaaaatccg cagttttac acaaatcgg atggattgt 
      caacgctac cttcaaatt attctatca aaatggaaa 
      agttataca cattaattt tagcgtctt aaatttggt 
      tatttttat ttattgtgt ctgtacaat tctattatg 
      aataaaatg gtttagaat gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY