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Gene information
General information
TC034616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19798657..19804716
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034616
    iBeetle screen iB_06722

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034616 RA
      MSDGEDDVP QLSASTFQA LQEFYKEQE 
      ERETRFLST PDENTTLDE NWQLSQFWY 
      DDKTTENLV NVALREVGP DGKIALVSC 
      PTLYKKMKE RVSDKGEVT LYEYDQRFS 
      VYGNDFVPY DYKSPLGVP REKASYYDL 
      VIADPPFLS EECLTKVAV TLKFLTKDK 
      IILCTGAVM EQFVERLLD LKKTPLKPQ 
      HRNNLGNEF YCYSNFKIE C
    CDS sequence
    • >TC034616 RA
      atgtcagat ggggaagat gatgtgccc cagttgtcg 
      gcaagtacg tttcaggcg cttcaggag ttttacaaa 
      gaacaggag gaaagggag acgcggttt ttgtcaaca 
      cctgatgaa aataccact ttagacgaa aactggcaa 
      ttgagtcag ttttggtac gatgataag accacagaa 
      aatttagtt aatgtagct ttaagagag gtgggtcca 
      gatggtaaa atagcactg gtttcgtgc ccaacactt 
      tacaaaaag atgaaggaa cgtgtaagt gacaagggg 
      gaagtaaca ctttatgaa tatgatcag cgttttagt 
      gtgtatggc aatgatttt gttccctat gattacaag 
      tcgcctcta ggggttccg agggaaaaa gctagttat 
      tatgatctt gtcatagct gatccaccg tttttgtca 
      gaggaatgt ttaaccaaa gttgcagtt acacttaaa 
      tttcttacg aaagacaaa atcattttg tgtacaggc 
      gcagtgatg gaacaattc gttgaacgc ctgctagac 
      ctgaagaaa actccctta aagccgcaa catcgaaat 
      aacttggga aatgaattt tactgttat tccaatttt 
      aagattgaa tgttaa
    mRNA sequence
    • >TC034616 RA
      ctaaaattt tcaaaaatt ttattaaaa aaaatgtca 
      gatggggaa gatgatgtg ccccagttg tcggcaagt 
      acgtttcag gcgcttcag gagttttac aaagaacag 
      gaggaaagg gagacgcgg tttttgtca acacctgat 
      gaaaatacc actttagac gaaaactgg caattgagt 
      cagttttgg tacgatgat aagaccaca gaaaattta 
      gttaatgta gctttaaga gaggtgggt ccagatggt 
      aaaatagca ctggtttcg tgcccaaca ctttacaaa 
      aagatgaag gaacgtgta agtgacaag ggggaagta 
      acactttat gaatatgat cagcgtttt agtgtgtat 
      ggcaatgat tttgttccc tatgattac aagtcgcct 
      ctaggggtt ccgagggaa aaagctagt tattatgat 
      cttgtcata gctgatcca ccgtttttg tcagaggaa 
      tgtttaacc aaagttgca gttacactt aaatttctt 
      acgaaagac aaaatcatt ttgtgtaca ggcgcagtg 
      atggaacaa ttcgttgaa cgcctgcta gacctgaag 
      aaaactccc ttaaagccg caacatcga aataacttg 
      ggaaatgaa ttttactgt tattccaat tttaagatt 
      gaatgttaa tgttaataa ttaaaacac gtttttaca 
      taggtacta ttttatttt ttggggggt gatatgtat 
      acatgaaat gtgtattta caacaattt aaagctatg 
      gggtgaaaa taactaatc acaatatca aaggcaagt 
      aactaaatt cactgtgac aaccaatga atggtggct 
      gtgctaata aataaaatt cggaatgtt taaaagaag 
      cataacttc tacaatatt nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnttca 
      tcttaaatt aaacagcaa aagaagttg tcaaacaac 
      attcactac aacgtttgt aattaattt atttagaat 
      ttatttgtt gcagtcgct gtgtttata ttgctgaaa 
      tcatttcac aagtaataa actttcatt cgattcaaa 
      gaaaaagca caacctgtg tgggtggga tttgttcaa 
      aacaaccgt tcttatagg cctctaaac cacattttc 
      actaaacta accaacaaa ttaatcacc aaacattca 
      tttttatta cggggtgaa ccttttata cagtgtgac 
      cggcacaaa tttggaaca atacagtac gacctcgat 
      aatccgaac taaatgttc ctacaaatg tactaaaca 
      cacaaaata cgttgcttc agttagtag aaattaaat 
      tagaaaaaa agactgaaa ccaagttat tttacaaaa 
      taaaaacat tcaaggtga ttttatttt acgacaaat 
      tatggcagt atgtcatcg taaaaacaa gtgaaaaca 
      gcttggtga acataaaat aaaataata acatacaca 
      gttttttgt cccaatgtt tagtagtca agttttcct 
      gttttgatt gtttggttt tgtttatta gttattata 
      atgcaataa tgttcagaa tttactata ttatttgtc 
      gccatagta tcttcgaaa tacttttag gaaaaaagt 
      tatttgctt aaattttta ctttcttca gtttacttt 
      ctgctttct tcaataaca aaatattgt ttgttcgaa 
      catttcgga cttaaaatt ttgttgtgt acttaataa 
      aagtttgta aaaaaaatc atattaaaa catatatac 
      gtagtattt ttggcgttc ggttgatga aaaattaaa 
      aagcatttt ttctactat tttataaac gggtaactg 
      aacctgtaa cactgtaat ttttatttt ttttaatgt 
      ttagagatt ttataacaa atgtactct tcaaaattc 
      acctgaatt cagattcaa aaaaaaaaa tataattag 
      gtatttagt taacttatt accaactga aattttact 
      cttaaggtg atattatct tacaagata aaacaagta 
      caaaatagt ttagtaaac ataacataa aataaagta 
      ataacttaa acagttttt tgtcccatt gtttaggaa 
      ccaagtttt cctgtttta attgtttgg ttttgttta 
      ttagttatt ttaatgcaa tgatgttca gaatttact 
      atattattt ttcacatta gtatcttcg atatacttc 
      taggaaaaa aagttaatt gctttaatt tttactttc 
      tgcgttctt cgataataa aatattgtt tgttcgaac 
      atttagttg ttaaggctt aaaactttt ttgtatatg 
      tttttctac tatttgaca aacgcctaa cttaacctg 
      caacactgc ttgtaattt taatttttt tagaaagtt 
      tttggggat tttataaca aattatctt tacttcaaa 
      atttacttt ataacttaa ttaattaag taaaaacac 
      tctctagat tttgatctc tggaaaagc tatatcatt 
      tgttttttg tttttgtgt atctgaatt cagagaaat 
      aaaaaatca taatgagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY