General information | |||
TC004404 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 19788411..19798457 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC004404 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06721 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
labium structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004404 RA
MISLRYTRN LFLRSFCIV YLFAFISFY IQIPGLYGD NGILPAKSL LENSKHKTL SAKVHYQPT LLWLAPYLG LDVNYALDV LALLGSFLA FTGFISQKF CTIPLFAGL WSLYFSLYQ VGQVFVSNN YDEFLLEAG FLVLLVAPL LPGRRKGSK VLPSDSISF WLVRWLLFR FLLTCGLLK FISGCPKWW TLSAFNYYF ETLPLPTPL SWYFHQIPS WYLKLFGVI ANVCEIGIP FLFFVPIRA VRITSFMIQ IFLQISIVL TGNYNFTNL LMVTLLLSL LDDQFFYGR RKPFTKWTF IEKVINILT YGALLLAVV KLYSLKLDG TIIESNIAF TKDQFEYVL KQGLTITIY LGLISLAVT ILRALSISL FDVQGSKVF TIITTILYS GIAVLLFFA STVPLASLH TASNSTVHP SVRTVYNRL HKLHAVNQY GLFPKMMGV DGRPEVILE GSDNVEGPW LEYNFLYKP GNVNHSLPF VAPYSPRLD WQMFWAAYN TYDKQPWIL SLTHRLLTG KAEVLALLD RHHSPFNQK PPKYIRGIL YKYKYSSWS QRSQPSWWV RQKVGEYFP AYTKESPAL VDYLKARNL LPTSSKQTV NPIWKQILD GVRYITGHL EATLLLWSV FTAGCAIIT TSAKK
- >TC004404 RA
atgatatct ctgcgatac accaggaat ctctttctg agatcgttc tgcatagtc tacctcttt gcatttatt tcgttctac atccaaatc cccggattg tacggcgat aatggaata ttaccggca aaatcgttg ttggaaaac agtaaacac aagacttta tcagccaaa gtacattat caacccaca ttactgtgg cttgccccc tacttgggc ttggatgtg aattatgcg ttggatgtg ttggcactg cttgggagc tttttagca tttacaggg tttatctcc caaaagttt tgtacaatt ccactcttt gctgggttg tggtcttta tatttctct ctttatcaa gttgggcaa gtttttgtg agcaataat tacgacgaa tttttacta gaggccggt tttcttgtt ctcctggtg gccccttta ctccctggc aggagaaag ggttcgaaa gttttaccc agcgattct attagtttc tggctggtg cgttggtta ctgtttcga tttttgtta acctgtggc ctattaaaa ttcatcagt ggttgcccc aagtggtgg actttatct gcatttaat tactatttt gaaacttta ccattacca acaccactc tcttggtac ttccatcaa attccgagc tggtacttg aaactgttc ggcgttatt gccaatgtt tgtgagatt ggtattccg tttttattc ttcgtcccg attcgagcg gtgcgcatc accagtttt atgatacaa atattccta caaatatct attgtactt actggcaat tataacttt accaacttg ttgatggta actctactt ttatcttta ctggacgac cagttcttt tacggccgc cgcaaaccc ttcaccaag tggactttt atcgaaaaa gtcatcaat attttgaca tatggcgct cttttattg gccgttgtt aagttatat tctttgaaa ctcgacggg acgataatt gaatccaat attgcattt acgaaggac cagtttgag tatgtcctc aagcaaggt ctcacaatc accatatac ttgggtttg atctcattg gctgttaca attctgcga gccctctcg atctccttg tttgacgtt cagggatca aaagtattc acaattatt acaacaatt ctgtattcg ggaatcgcc gtacttcta tttttcgct agtaccgtt cctctcgcg tctttacac acagcatcc aattcaacc gttcatcca agtgtccgc actgtatat aaccgactt cataaactg catgctgtc aaccagtat ggacttttc ccaaaaatg atgggagtg gatggtcgt cctgaagtt atccttgaa ggttccgac aatgttgag ggcccatgg ctcgaatat aacttcctg tacaagccg ggaaatgtg aaccactcc ttacctttt gttgcacca tactctccc aggttggac tggcagatg ttctgggcg gcttacaac acttacgac aagcagcct tggattttg agtttaact caccgatta ttgacgggg aaagccgaa gttttggct cttttggac agacaccat tcaccgttc aaccaaaaa ccgccaaaa tacatccga ggaattctc tataaatac aagtattcg tcgtggagt caaaggtcg cagccctcg tggtgggtt cgccaaaaa gtgggtgaa tatttcccc gcctacaca aaagagtct ccagctttg gtggattac ttaaaggct aggaacctc ttgcctacg tcttcgaaa cagactgtc aaccccatt tggaagcag attttggat ggggtgagg tatattaca gggcattta gaagctact ttgcttttg tggtcagtt tttacggct ggttgtgcc attattaca acttctgcc aagaaataa
- >TC004404 RA
aataatctg ttgattttt tcactaaac gtcattttt caaaatcca aaattttag tatgtcgag cttagaaag caagaaaaa gccctccaa acagtcaaa aaataagga aaagaaaga attaggtga caccgagct tccccgcaa accaaaaat taaaaagga acaaaaagg cacacaaga gacaaaagt gaagaaagg gaccccgaa gggttcttt ggttggtgg tggcattaa gaaaaagtc caacaagaa tgagtaaac aagacacac attattatt atcattaaa cacaaagtg tgtgcatat aatcaatac atggcatgt aaatatact gttacattt aaaaacgta tcaaatatt tacttttgc taaatttgt aattgtgtt cacatcatt gttgggaca ggaatttcc aaaattcag ctacttgtg ctagttttt aattaactt aatgcctag aagaatcca ccaattttc ataaaaccg aggaaatgt gatgatgtt aaacataaa aaacagtta ttttattcg gattcgatc attcctgtt tatattaca aactagcgc tccttgtcc cacctatgt tgaggtgca tgcttggct ggttgctgt gtccttgtc tcttgctgg gcgtttgtt ggttcgagt tggttgcgt tgcgtcgct ttattcttt cagatcaca tcatcacta tcatcaaat acgagagaa gtactcgtg ttaagttgt tattaatgt gttgtttat tgtttacat gttgagttc aatctccag aagtggatt tttttctat cggcttgtt aattatcgg tgaacaatg atatctctg cgatacacc aggaatctc tttctgaga tcgttctgc atagtctac ctctttgca tttatttcg ttctacatc caaatcccc ggattgtac ggcgataat ggaatatta ccggcaaaa tcgttgttg gaaaacagt aaacacaag actttatca gccaaagta cattatcaa cccacatta ctgtggctt gccccctac ttgggcttg gatgtgaat tatgcgttg gatgtgttg gcactgctt gggagcttt ttagcattt acagggttt atctcccaa aagttttgt acaattcca ctctttgct gggttgtgg tctttatat ttctctctt tatcaagtt gggcaagtt tttgtgagc aataattac gacgaattt ttactagag gccggtttt cttgttctc ctggtggcc cctttactc cctggcagg agaaagggt tcgaaagtt ttacccagc gattctatt agtttctgg ctggtgcgt tggttactg tttcgattt ttgttaacc tgtggccta ttaaaattc atcagtggt tgccccaag tggtggact ttatctgca tttaattac tattttgaa actttacca ttaccaaca ccactctct tggtacttc catcaaatt ccgagctgg tacttgaaa ctgttcggc gttattgcc aatgtttgt gagattggt attccgttt ttattcttc gtcccgatt cgagcggtg cgcatcacc agttttatg atacaaata ttcctacaa atatctatt gtacttact ggcaattat aactttacc aacttgttg atggtaact ctactttta tctttactg gacgaccag ttcttttac ggccgccgc aaacccttc accaagtgg acttttatc gaaaaagtc atcaatatt ttgacatat ggcgctctt ttattggcc gttgttaag ttatattct ttgaaactc gacgggacg ataattgaa tccaatatt gcatttacg aaggaccag tttgagtat gtcctcaag caaggtctc acaatcacc atatacttg ggtttgatc tcattggct gttacaatt ctgcgagcc ctctcgatc tccttgttt gacgttcag ggatcaaaa gtattcaca attattaca acaattctg tattcggga atcgccgta cttctattt ttcgctagt accgttcct ctcgcgtct ttacacaca gcatccaat tcaaccgtt catccaagt gtccgcact gtatataac cgacttcat aaactgcat gctgtcaac cagtatgga cttttccca aaaatgatg ggagtggat ggtcgtcct gaagttatc cttgaaggt tccgacaat gttgagggc ccatggctc gaatataac ttcctgtac aagccggga aatgtgaac cactcctta ccttttgtt gcaccatac tctcccagg ttggactgg cagatgttc tgggcggct tacaacact tacgacaag cagccttgg attttgagt ttaactcac cgattattg acggggaaa gccgaagtt ttggctctt ttggacaga caccattca ccgttcaac caaaaaccg ccaaaatac atccgagga attctctat aaatacaag tattcgtcg tggagtcaa aggtcgcag ccctcgtgg tgggttcgc caaaaagtg ggtgaatat ttccccgcc tacacaaaa gagtctcca gctttggtg gattactta aaggctagg aacctcttg cctacgtct tcgaaacag actgtcaac cccatttgg aagcagatt ttggatggg gtgaggtat attacaggg catttagaa gctactttg cttttgtgg tcagttttt acggctggt tgtgccatt attacaact tctgccaag aaataagaa ctgaatttg agtcactag tttttattt tctcattga aaagtttca gttaaggat ctttatggt gtgattttt tttattttt atatgtgca cacactaat ttatcgagt aatttcacc cctccattt agatcagaa tatgtcata cgatattgt tatcaggtt tgtcccgtg tgaagtttt taaatgtaa tgtttttgt tagtaaagt gcaaatata tcatttatt taaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
