Status
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Gene information
General information
TC004404 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19788411..19798457
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004404
      iBeetle screen iB_06721

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      labium structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      thorax partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      thorax fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004404 RA
        MISLRYTRN LFLRSFCIV YLFAFISFY IQIPGLYGD 
        NGILPAKSL LENSKHKTL SAKVHYQPT LLWLAPYLG 
        LDVNYALDV LALLGSFLA FTGFISQKF CTIPLFAGL 
        WSLYFSLYQ VGQVFVSNN YDEFLLEAG FLVLLVAPL 
        LPGRRKGSK VLPSDSISF WLVRWLLFR FLLTCGLLK 
        FISGCPKWW TLSAFNYYF ETLPLPTPL SWYFHQIPS 
        WYLKLFGVI ANVCEIGIP FLFFVPIRA VRITSFMIQ 
        IFLQISIVL TGNYNFTNL LMVTLLLSL LDDQFFYGR 
        RKPFTKWTF IEKVINILT YGALLLAVV KLYSLKLDG 
        TIIESNIAF TKDQFEYVL KQGLTITIY LGLISLAVT 
        ILRALSISL FDVQGSKVF TIITTILYS GIAVLLFFA 
        STVPLASLH TASNSTVHP SVRTVYNRL HKLHAVNQY 
        GLFPKMMGV DGRPEVILE GSDNVEGPW LEYNFLYKP 
        GNVNHSLPF VAPYSPRLD WQMFWAAYN TYDKQPWIL 
        SLTHRLLTG KAEVLALLD RHHSPFNQK PPKYIRGIL 
        YKYKYSSWS QRSQPSWWV RQKVGEYFP AYTKESPAL 
        VDYLKARNL LPTSSKQTV NPIWKQILD GVRYITGHL 
        EATLLLWSV FTAGCAIIT TSAKK
      CDS sequence
      • >TC004404 RA
        atgatatct ctgcgatac accaggaat ctctttctg 
        agatcgttc tgcatagtc tacctcttt gcatttatt 
        tcgttctac atccaaatc cccggattg tacggcgat 
        aatggaata ttaccggca aaatcgttg ttggaaaac 
        agtaaacac aagacttta tcagccaaa gtacattat 
        caacccaca ttactgtgg cttgccccc tacttgggc 
        ttggatgtg aattatgcg ttggatgtg ttggcactg 
        cttgggagc tttttagca tttacaggg tttatctcc 
        caaaagttt tgtacaatt ccactcttt gctgggttg 
        tggtcttta tatttctct ctttatcaa gttgggcaa 
        gtttttgtg agcaataat tacgacgaa tttttacta 
        gaggccggt tttcttgtt ctcctggtg gccccttta 
        ctccctggc aggagaaag ggttcgaaa gttttaccc 
        agcgattct attagtttc tggctggtg cgttggtta 
        ctgtttcga tttttgtta acctgtggc ctattaaaa 
        ttcatcagt ggttgcccc aagtggtgg actttatct 
        gcatttaat tactatttt gaaacttta ccattacca 
        acaccactc tcttggtac ttccatcaa attccgagc 
        tggtacttg aaactgttc ggcgttatt gccaatgtt 
        tgtgagatt ggtattccg tttttattc ttcgtcccg 
        attcgagcg gtgcgcatc accagtttt atgatacaa 
        atattccta caaatatct attgtactt actggcaat 
        tataacttt accaacttg ttgatggta actctactt 
        ttatcttta ctggacgac cagttcttt tacggccgc 
        cgcaaaccc ttcaccaag tggactttt atcgaaaaa 
        gtcatcaat attttgaca tatggcgct cttttattg 
        gccgttgtt aagttatat tctttgaaa ctcgacggg 
        acgataatt gaatccaat attgcattt acgaaggac 
        cagtttgag tatgtcctc aagcaaggt ctcacaatc 
        accatatac ttgggtttg atctcattg gctgttaca 
        attctgcga gccctctcg atctccttg tttgacgtt 
        cagggatca aaagtattc acaattatt acaacaatt 
        ctgtattcg ggaatcgcc gtacttcta tttttcgct 
        agtaccgtt cctctcgcg tctttacac acagcatcc 
        aattcaacc gttcatcca agtgtccgc actgtatat 
        aaccgactt cataaactg catgctgtc aaccagtat 
        ggacttttc ccaaaaatg atgggagtg gatggtcgt 
        cctgaagtt atccttgaa ggttccgac aatgttgag 
        ggcccatgg ctcgaatat aacttcctg tacaagccg 
        ggaaatgtg aaccactcc ttacctttt gttgcacca 
        tactctccc aggttggac tggcagatg ttctgggcg 
        gcttacaac acttacgac aagcagcct tggattttg 
        agtttaact caccgatta ttgacgggg aaagccgaa 
        gttttggct cttttggac agacaccat tcaccgttc 
        aaccaaaaa ccgccaaaa tacatccga ggaattctc 
        tataaatac aagtattcg tcgtggagt caaaggtcg 
        cagccctcg tggtgggtt cgccaaaaa gtgggtgaa 
        tatttcccc gcctacaca aaagagtct ccagctttg 
        gtggattac ttaaaggct aggaacctc ttgcctacg 
        tcttcgaaa cagactgtc aaccccatt tggaagcag 
        attttggat ggggtgagg tatattaca gggcattta 
        gaagctact ttgcttttg tggtcagtt tttacggct 
        ggttgtgcc attattaca acttctgcc aagaaataa
      mRNA sequence
      • >TC004404 RA
        aataatctg ttgattttt tcactaaac gtcattttt 
        caaaatcca aaattttag tatgtcgag cttagaaag 
        caagaaaaa gccctccaa acagtcaaa aaataagga 
        aaagaaaga attaggtga caccgagct tccccgcaa 
        accaaaaat taaaaagga acaaaaagg cacacaaga 
        gacaaaagt gaagaaagg gaccccgaa gggttcttt 
        ggttggtgg tggcattaa gaaaaagtc caacaagaa 
        tgagtaaac aagacacac attattatt atcattaaa 
        cacaaagtg tgtgcatat aatcaatac atggcatgt 
        aaatatact gttacattt aaaaacgta tcaaatatt 
        tacttttgc taaatttgt aattgtgtt cacatcatt 
        gttgggaca ggaatttcc aaaattcag ctacttgtg 
        ctagttttt aattaactt aatgcctag aagaatcca 
        ccaattttc ataaaaccg aggaaatgt gatgatgtt 
        aaacataaa aaacagtta ttttattcg gattcgatc 
        attcctgtt tatattaca aactagcgc tccttgtcc 
        cacctatgt tgaggtgca tgcttggct ggttgctgt 
        gtccttgtc tcttgctgg gcgtttgtt ggttcgagt 
        tggttgcgt tgcgtcgct ttattcttt cagatcaca 
        tcatcacta tcatcaaat acgagagaa gtactcgtg 
        ttaagttgt tattaatgt gttgtttat tgtttacat 
        gttgagttc aatctccag aagtggatt tttttctat 
        cggcttgtt aattatcgg tgaacaatg atatctctg 
        cgatacacc aggaatctc tttctgaga tcgttctgc 
        atagtctac ctctttgca tttatttcg ttctacatc 
        caaatcccc ggattgtac ggcgataat ggaatatta 
        ccggcaaaa tcgttgttg gaaaacagt aaacacaag 
        actttatca gccaaagta cattatcaa cccacatta 
        ctgtggctt gccccctac ttgggcttg gatgtgaat 
        tatgcgttg gatgtgttg gcactgctt gggagcttt 
        ttagcattt acagggttt atctcccaa aagttttgt 
        acaattcca ctctttgct gggttgtgg tctttatat 
        ttctctctt tatcaagtt gggcaagtt tttgtgagc 
        aataattac gacgaattt ttactagag gccggtttt 
        cttgttctc ctggtggcc cctttactc cctggcagg 
        agaaagggt tcgaaagtt ttacccagc gattctatt 
        agtttctgg ctggtgcgt tggttactg tttcgattt 
        ttgttaacc tgtggccta ttaaaattc atcagtggt 
        tgccccaag tggtggact ttatctgca tttaattac 
        tattttgaa actttacca ttaccaaca ccactctct 
        tggtacttc catcaaatt ccgagctgg tacttgaaa 
        ctgttcggc gttattgcc aatgtttgt gagattggt 
        attccgttt ttattcttc gtcccgatt cgagcggtg 
        cgcatcacc agttttatg atacaaata ttcctacaa 
        atatctatt gtacttact ggcaattat aactttacc 
        aacttgttg atggtaact ctactttta tctttactg 
        gacgaccag ttcttttac ggccgccgc aaacccttc 
        accaagtgg acttttatc gaaaaagtc atcaatatt 
        ttgacatat ggcgctctt ttattggcc gttgttaag 
        ttatattct ttgaaactc gacgggacg ataattgaa 
        tccaatatt gcatttacg aaggaccag tttgagtat 
        gtcctcaag caaggtctc acaatcacc atatacttg 
        ggtttgatc tcattggct gttacaatt ctgcgagcc 
        ctctcgatc tccttgttt gacgttcag ggatcaaaa 
        gtattcaca attattaca acaattctg tattcggga 
        atcgccgta cttctattt ttcgctagt accgttcct 
        ctcgcgtct ttacacaca gcatccaat tcaaccgtt 
        catccaagt gtccgcact gtatataac cgacttcat 
        aaactgcat gctgtcaac cagtatgga cttttccca 
        aaaatgatg ggagtggat ggtcgtcct gaagttatc 
        cttgaaggt tccgacaat gttgagggc ccatggctc 
        gaatataac ttcctgtac aagccggga aatgtgaac 
        cactcctta ccttttgtt gcaccatac tctcccagg 
        ttggactgg cagatgttc tgggcggct tacaacact 
        tacgacaag cagccttgg attttgagt ttaactcac 
        cgattattg acggggaaa gccgaagtt ttggctctt 
        ttggacaga caccattca ccgttcaac caaaaaccg 
        ccaaaatac atccgagga attctctat aaatacaag 
        tattcgtcg tggagtcaa aggtcgcag ccctcgtgg 
        tgggttcgc caaaaagtg ggtgaatat ttccccgcc 
        tacacaaaa gagtctcca gctttggtg gattactta 
        aaggctagg aacctcttg cctacgtct tcgaaacag 
        actgtcaac cccatttgg aagcagatt ttggatggg 
        gtgaggtat attacaggg catttagaa gctactttg 
        cttttgtgg tcagttttt acggctggt tgtgccatt 
        attacaact tctgccaag aaataagaa ctgaatttg 
        agtcactag tttttattt tctcattga aaagtttca 
        gttaaggat ctttatggt gtgattttt tttattttt 
        atatgtgca cacactaat ttatcgagt aatttcacc 
        cctccattt agatcagaa tatgtcata cgatattgt 
        tatcaggtt tgtcccgtg tgaagtttt taaatgtaa 
        tgtttttgt tagtaaagt gcaaatata tcatttatt 
        taaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY