Status
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Gene information
General information
TC004408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19780189..19785949
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004408
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06720

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    4
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004408 RA
      MEDEQDLKK MNDFIKNQK SFQQSFQVE FQDFVDFKQ 
      DDTSDLEFE EELREEKVN YQLEEPTSG QLPSLTLTE 
      DEKQVIDSC TDPELKRLL VLNRSRNIQ LLKLQKKVQ 
      DLLQQCEVE LCDKNNILK NSEPKQGLH QTYIWKLAA 
      PYFKDRKFF PSPLNEEAV KKRARNELS VYDLVPSPQ 
      WTPIEFDRL YNAVRCNYN ISRQMEIMS KINALKAKV 
      SEGEDESKI EEIVHLKEE LERVKNDEK IPPLNSNEN 
      INWYKVSET FLKDKHTPL ECQSVWHTL IHPQINKSD 
      WSDEENKKL ETIAKRHSY QNWDLIASE LDNSRTGFT 
      TCLHYFSKL CDKFKKSKF TPGEDAFLL EVVDSYKIG 
      NFIPWNKVV CHFDNRSRH QLYHRYTYF LSQNHVKKG 
      KFSEAEDIL LIILVNKFG RNFKKCAEF MPHRSQIQL 
      KSRYNSNLQ RSIKKGTFT TEDDEIIYE YAQEHGEKN 
      WGGLTDRLK RCSAQIRQR YKLIKTFLH ENPDSEIRD 
      IPKRKHRFN EVSEDQYSF LNFIAEQYK GSDEIPTLS 
      MIEESLQAE HKHSEAKND RTESKFKNV DTLLTDLFR 
      NTGTTSHKF VSESSLADA TENAAEILT TLGVKLDIP 
      KDLQLKSRL DNIDLEILE RLSKKNDSV PTVSGLVPP 
      NLCTLVGLR RLIIQNKEY KQQIKGRKT KLAWPMEQN 
      LLKLSESAR AEVLHHREL FAKRFDTLF DWSAVLSLE 
      KPNQFDIPK KRASLQPND GTVKRRKIH VTDLAKISK 
      DSSQLRLVD KSVIERLMK TRNNVNIIR IETMTGPDG 
      RVKPVLAQA NGAVATSSG EEVGHEDVQ DVEKLNVVL 
      KSMKSEAVS ESGSEFQAI KGDVQLLKQ ILQYEKVKV 
      KAEPDG
    CDS sequence
    • >TC004408 RA
      atggaagac gaacaagac cttaagaag atgaacgat 
      tttataaaa aatcaaaag tcgtttcaa caaagtttt 
      caagtggaa tttcaagat ttcgttgac ttcaaacag 
      gacgacaca agtgatttg gaatttgaa gaagaatta 
      agagaagag aaagtgaat tatcaactt gaggaacct 
      actagcggt caattacca agtcttacg ttaacagaa 
      gacgaaaaa caagtgata gactcatgc actgaccct 
      gagctgaaa cgactgctt gtcctaaac cggtcgaga 
      aacatccaa cttttaaag ctccagaaa aaagtgcaa 
      gatctcttg cagcagtgt gaagtagag ctttgtgac 
      aaaaacaac attttaaaa aactcagag ccgaaacaa 
      ggattgcac cagacgtac atttggaaa cttgcagca 
      ccgtatttt aaggacagg aaatttttt ccgtcgccc 
      cttaatgag gaagctgtt aaaaagcgt gcgaggaat 
      gagttgagt gtttatgat ttggtgcca tcaccacag 
      tggacgccg atagagttt gataggttg tacaatgca 
      gttagatgc aattataac ataagtagg cagatggaa 
      attatgtcc aagataaac gcactgaag gcgaaagta 
      tcggaggga gaagacgaa agtaagata gaagaaatt 
      gttcatttg aaagaagaa ctggaaaga gttaaaaat 
      gatgagaaa attccgcca ctgaactca aacgagaat 
      attaattgg tacaaagtg tctgagact tttttgaaa 
      gataaacac actccactg gaatgccag tccgtttgg 
      cacacgcta atccacccc caaataaac aaatcagac 
      tggtctgac gaggaaaac aaaaaactc gaaacaatc 
      gcaaaacgc cactcttac caaaactgg gacctgata 
      gcctcagaa ctcgacaac agccgcaca ggcttcacc 
      acctgtctc cactacttc agcaaatta tgcgacaag 
      ttcaaaaaa agcaaattc acgcctggc gaagacgca 
      tttctgtta gaagttgta gacagttac aaaatcggc 
      aacttcatc ccttggaac aaagtcgtc tgccacttc 
      gacaaccgc tcccgacac caactctac catcgctac 
      acctacttc ctgtcgcaa aaccacgtc aaaaaaggg 
      aaattttca gaggccgag gacatcctg ctgattatc 
      cttgtgaac aaattcggg cggaatttc aaaaagtgc 
      gccgaattc atgccacat aggtcacaa atccagctg 
      aaaagtcgc tacaattct aatctgcaa cgcagcata 
      aaaaaaggg actttcaca acggaagat gatgaaatt 
      atctacgaa tacgcgcaa gaacatggc gagaaaaac 
      tggggcggt ttaactgat cggttgaag cgctgcagt 
      gcacaaatc cgacagaga tacaagtta attaaaacg 
      tttttacac gaaaatccc gattccgaa atcagggat 
      attccaaaa cggaagcat cgcttcaat gaagtgagt 
      gaagaccag tacagtttt ttgaatttt attgccgaa 
      cagtacaag ggatcggac gaaattccc actctctcc 
      atgatcgaa gagagcctt caagcggaa cataaacac 
      agtgaggca aaaaacgac cgaactgaa tcaaaattc 
      aagaacgta gacacctta cttactgac ctgtttcga 
      aacactgga acaacatca cataaattc gtttctgag 
      tcgagtctt gctgatgcg accgaaaat gcggcagaa 
      atactcaca actcttgga gttaagtta gatatacca 
      aaggacttg cagttgaaa tcgcgtctg gataatata 
      gacttggaa attttggaa cgtttgtcg aagaaaaac 
      gattcagtt ccgacagtt agtggttta gttccgcct 
      aatttgtgc actttggtc ggtttaagg cgcttaata 
      atccaaaac aaggaatac aagcagcag attaaaggc 
      cgaaaaaca aaactagcc tggcccatg gaacaaaac 
      ttgctgaag ttgagtgag tcagcacgg gctgaagtc 
      ttgcaccat cgagagttg tttgcgaaa cggtttgac 
      acgcttttc gactggagt gcggttttg tcgttggaa 
      aaaccgaac cagttcgat attcctaaa aaacgagca 
      agtctccag ccgaacgat ggcaccgtc aaacgaaga 
      aaaatccac gtgactgat cttgcgaaa ataagcaag 
      gacagttcg cagctgcga ttggttgat aaaagtgtg 
      attgaacgc ttgatgaaa accagaaat aatgtcaat 
      attataagg attgagacg atgacgggg cccgatggg 
      agggttaag ccagtgcta gcacaagct aatggtgca 
      gttgcgacg tcaagtggt gaggaggtt ggtcatgaa 
      gatgtgcaa gatgttgag aaattgaac gttgtgctg 
      aaatcgatg aagtcggaa gctgtgtcc gagtcgggg 
      agcgagttt caagcgatt aaaggcgat gtgcagctc 
      cttaagcag attctacag tatgagaaa gttaaggtc 
      aaggcggag ccggatggg tag
    mRNA sequence
    • >TC004408 RA
      taataaaac aaaaactac aattaaaat cattatatg 
      gagcggtgg aagaattcg ggtgaatga gccacactg 
      tgagccgag cattcgtcc gaaagtaaa aaattgttt 
      attgttgtt ttttacaaa aataccaac aaaaaagta 
      aacaaaatg ctgtgtgtg tttaaagtg attgttatc 
      aaaagttaa taatattat ttgataccc gggcgagtc 
      taattgact acatttaaa ccctaaaat ttttgaggt 
      tagctaatt ttgtcggtg ttgtctttg tgtggtaaa 
      aaaattaaa ttcgtaaca atggaagac gaacaagac 
      cttaagaag atgaacgat tttataaaa aatcaaaag 
      tcgtttcaa caaagtttt caagtggaa tttcaagat 
      ttcgttgac ttcaaacag gacgacaca agtgatttg 
      gaatttgaa gaagaatta agagaagag aaagtgaat 
      tatcaactt gaggaacct actagcggt caattacca 
      agtcttacg ttaacagaa gacgaaaaa caagtgata 
      gactcatgc actgaccct gagctgaaa cgactgctt 
      gtcctaaac cggtcgaga aacatccaa cttttaaag 
      ctccagaaa aaagtgcaa gatctcttg cagcagtgt 
      gaagtagag ctttgtgac aaaaacaac attttaaaa 
      aactcagag ccgaaacaa ggattgcac cagacgtac 
      atttggaaa cttgcagca ccgtatttt aaggacagg 
      aaatttttt ccgtcgccc cttaatgag gaagctgtt 
      aaaaagcgt gcgaggaat gagttgagt gtttatgat 
      ttggtgcca tcaccacag tggacgccg atagagttt 
      gataggttg tacaatgca gttagatgc aattataac 
      ataagtagg cagatggaa attatgtcc aagataaac 
      gcactgaag gcgaaagta tcggaggga gaagacgaa 
      agtaagata gaagaaatt gttcatttg aaagaagaa 
      ctggaaaga gttaaaaat gatgagaaa attccgcca 
      ctgaactca aacgagaat attaattgg tacaaagtg 
      tctgagact tttttgaaa gataaacac actccactg 
      gaatgccag tccgtttgg cacacgcta atccacccc 
      caaataaac aaatcagac tggtctgac gaggaaaac 
      aaaaaactc gaaacaatc gcaaaacgc cactcttac 
      caaaactgg gacctgata gcctcagaa ctcgacaac 
      agccgcaca ggcttcacc acctgtctc cactacttc 
      agcaaatta tgcgacaag ttcaaaaaa agcaaattc 
      acgcctggc gaagacgca tttctgtta gaagttgta 
      gacagttac aaaatcggc aacttcatc ccttggaac 
      aaagtcgtc tgccacttc gacaaccgc tcccgacac 
      caactctac catcgctac acctacttc ctgtcgcaa 
      aaccacgtc aaaaaaggg aaattttca gaggccgag 
      gacatcctg ctgattatc cttgtgaac aaattcggg 
      cggaatttc aaaaagtgc gccgaattc atgccacat 
      aggtcacaa atccagctg aaaagtcgc tacaattct 
      aatctgcaa cgcagcata aaaaaaggg actttcaca 
      acggaagat gatgaaatt atctacgaa tacgcgcaa 
      gaacatggc gagaaaaac tggggcggt ttaactgat 
      cggttgaag cgctgcagt gcacaaatc cgacagaga 
      tacaagtta attaaaacg tttttacac gaaaatccc 
      gattccgaa atcagggat attccaaaa cggaagcat 
      cgcttcaat gaagtgagt gaagaccag tacagtttt 
      ttgaatttt attgccgaa cagtacaag ggatcggac 
      gaaattccc actctctcc atgatcgaa gagagcctt 
      caagcggaa cataaacac agtgaggca aaaaacgac 
      cgaactgaa tcaaaattc aagaacgta gacacctta 
      cttactgac ctgtttcga aacactgga acaacatca 
      cataaattc gtttctgag tcgagtctt gctgatgcg 
      accgaaaat gcggcagaa atactcaca actcttgga 
      gttaagtta gatatacca aaggacttg cagttgaaa 
      tcgcgtctg gataatata gacttggaa attttggaa 
      cgtttgtcg aagaaaaac gattcagtt ccgacagtt 
      agtggttta gttccgcct aatttgtgc actttggtc 
      ggtttaagg cgcttaata atccaaaac aaggaatac 
      aagcagcag attaaaggc cgaaaaaca aaactagcc 
      tggcccatg gaacaaaac ttgctgaag ttgagtgag 
      tcagcacgg gctgaagtc ttgcaccat cgagagttg 
      tttgcgaaa cggtttgac acgcttttc gactggagt 
      gcggttttg tcgttggaa aaaccgaac cagttcgat 
      attcctaaa aaacgagca agtctccag ccgaacgat 
      ggcaccgtc aaacgaaga aaaatccac gtgactgat 
      cttgcgaaa ataagcaag gacagttcg cagctgcga 
      ttggttgat aaaagtgtg attgaacgc ttgatgaaa 
      accagaaat aatgtcaat attataagg attgagacg 
      atgacgggg cccgatggg agggttaag ccagtgcta 
      gcacaagct aatggtgca gttgcgacg tcaagtggt 
      gaggaggtt ggtcatgaa gatgtgcaa gatgttgag 
      aaattgaac gttgtgctg aaatcgatg aagtcggaa 
      gctgtgtcc gagtcgggg agcgagttt caagcgatt 
      aaaggcgat gtgcagctc cttaagcag attctacag 
      tatgagaaa gttaaggtc aaggcggag ccggatggg 
      taggtgcgt tgctttagt ttatgttga accaaagta 
      ttaataaat agtcgattt tcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY