| General information | |||
| TC032600
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 19751612..19762303 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032600 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06718 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032600 RA
MYCDKAILD PRFDVPNKI LRNLSEDPT FKASEYFDK FASLETNLD RINFVYQNF EKFEAYPPL ISDNKDDLL AGTARETGN GHFGRGEFR KALDCYTRS IQTANTKEV MALGFGNRS AALLKLGFY AECLTDINR ALKNEYPDK LKFKLLARQ NFCKGQTIV ENNAYDPVP TFPAFEKNA SIQCARNCI EIMQNERYG RHVVATRNI EMGEILAIE KPYASIVTD SVSVYCHEC LKLCYNMIP CDKCTKALY CSDNCKDKA YESYHKYEC PIHLSLDPL LIDSSKRLA LRIALISRN EWAGSLLNE SPDEMYCSD RFKEVFNLD QNVRQRFTH DLFGRTTIA CGVFYLIKK YTTFLQEYD EDRFKEILL SLLLICSTN TVRVNEVSS TLGEYDVCG FACSHYPFF SMFNHSCWP NVCRSYHGS QMVLRAIRT IKKGEQCFV TYGPSYLSD NIVGRQAFL FFHYFFNCG CKACVESWP KKSIVDMFI KSCREQGEN FFCALLRRK LVREADADV ARKHIPSLL VKAKFEELY LPPAEDFFV NDTLDFCYS VLACGVFYL IKKYTTFLQ EYDDGDRFK EILLSLLLI CSTNTVRVN EVSSTLGEY DVCGFASSH YPFFSMFNH SCWPNVCRS YHGSQMVLR AIRTIKKGE QCFVTYGPS YLSENIVGR QAFLFFHYF FNCGCKACV ESWPKKSIV DMFIKSCRE QGENFFCAL LRRKLNLKN CTFRLLRIS S
- >TC032600 RA
atgtattgt gataaggca atattggac ccaaggttc gatgtacca aataaaata cttcgaaac ttgtcggag gaccccact ttcaaagca tccgagtat ttcgacaag tttgcttca cttgaaacg aatttagat cgaataaat tttgtctac caaaatttc gaaaaattt gaagcatat cctccttta ataagtgac aataaggat gacctgctg gctggaaca gcaagggaa actggaaat ggacatttt ggacgtggc gaattcaga aaagctctg gattgttac acgaggagt atccaaaca gctaatact aaggaagta atggcactg ggttttggt aatagatca gcagcttta ttgaaattg ggattctac gcggagtgt ttgacggac ataaatcgc gcccttaaa aatgagtac cccgacaag ctaaaattt aaattactg gcgagacaa aatttctgc aaaggccaa acaatcgtg gaaaataac gcttacgac ccagttcca acatttccc gcttttgaa aaaaatgct tccattcag tgtgcaaga aactgcatt gaaatcatg caaaatgag cggtacgga cgccacgta gttgcaacc agaaacatc gaaatgggg gaaattcta gcaattgaa aaaccgtac gcttctatc gtaacagat tcagtttct gtttactgc cacgagtgt ttgaaactt tgttataac atgatacct tgcgataaa tgcacgaag gcattgtat tgcagtgat aattgcaag gacaaagct tacgaaagc taccacaag tacgaatgt ccgattcac ttgtccctt gatccttta ctaatcgac tcttcgaaa cgattggct ttaagaatt gcccttatt tcgagaaac gaatgggct gggagtctc ctaaacgaa agtccagat gaaatgtat tgttctgac agatttaag gaagtgttc aatttggac caaaacgta cgacaacgc ttcactcac gacttattc ggtcgaact accatagct tgcggagtt ttctatttg attaaaaaa tacaccacg tttttgcaa gaatatgac gaggaccgt ttcaaagaa atcctcctg tcgttactt ttgatctgt agtactaac acagtgcgt gttaacgaa gttagttcc actttggga gaatacgac gtttgtggt tttgcctgt tcacattat ccgtttttt tccatgttc aatcactcc tgctggccc aatgtttgt cgctcttac catggctcc caaatggtc cttagggcc attcgtacc attaaaaag ggcgagcag tgttttgtg acctatggt cctagttat ttgtcggat aatatagtg gggaggcag gcttttctg ttctttcat tactttttc aattgcggg tgtaaagcg tgtgtggaa agctggcct aagaagagt atcgtagat atgtttatt aaaagttgt cgagagcaa ggtgaaaac tttttctgt gctcttctg agacggaag ttagtaaga gaagctgat gcggatgtt gccagaaag cacattcct tctttgcta gttaaggct aaatttgaa gaattgtat cttccgcct gctgaggat ttcttcgtg aatgataca ttggatttt tgttacagt gtgcttgct tgcggagtt ttctatttg attaaaaaa tacaccacg tttttgcaa gaatatgac gatggggac cgtttcaaa gaaatcctc ctgtcgtta cttttgatc tgtagtact aacacagtg cgtgttaac gaagttagt tccacattg ggagaatac gacgtttgt ggttttgcc tcttcacat tacccattt ttttccatg ttcaatcac tcctgctgg cccaatgtt tgccgctct taccatggc tcccaaatg gtccttagg gccattcgt accattaaa aagggcgag cagtgtttt gtgacctat ggtcctagt tatttgtcg gagaatata gtggggagg caggctttt ctcttcttt cattacttt ttcaattgc gggtgtaaa gcgtgtgtg gaaagctgg cctaagaag agtatcgta gatatgttt attaaaagt tgtcgagag caaggtgaa aactttttc tgtgctctt ctgagacgg aagctaaat ttgaagaat tgtaccttc cgcctgctg aggatttct tcgtga
- >TC032600 RA
cgtccagtc gggtgtttc ccccagcta cagtttgag acgcagttg gctttgtgc ttgtggtgt cttgatcaa caatagttc agcatgtat tgtgataag gcaatattg gacccaagg ttcgatgta ccaaataaa atacttcga aacttgtcg gaggacccc actttcaaa gcatccgag tatttcgac aagtttgct tcacttgaa acgaattta gatcgaata aattttgtc taccaaaat ttcgaaaaa tttgaagca tatcctcct ttaataagt gacaataag gatgacctg ctggctgga acagcaagg gaaactgga aatggacat tttggacgt ggcgaattc agaaaagct ctggattgt tacacgagg agtatccaa acagctaat actaaggaa gtaatggca ctgggtttt ggtaataga tcagcagct ttattgaaa ttgggattc tacgcggag tgtttgacg gacataaat cgcgccctt aaaaatgag taccccgac aagctaaaa tttaaatta ctggcgaga caaaatttc tgcaaaggc caaacaatc gtggaaaat aacgcttac gacccagtt ccaacattt cccgctttt gaaaaaaat gcttccatt cagtgtgca agaaactgc attgaaatc atgcaaaat gagcggtac ggacgccac gtagttgca accagaaac atcgaaatg ggggaaatt ctagcaatt gaaaaaccg tacgcttct atcgtaaca gattcagtt tctgtttac tgccacgag tgtttgaaa ctttgttat aacatgata ccttgcgat aaatgcacg aaggcattg tattgcagt gataattgc aaggacaaa gcttacgaa agctaccac aagtacgaa tgtccgatt cacttgtcc cttgatcct ttactaatc gactcttcg aaacgattg gctttaaga attgccctt atttcgaga aacgaatgg gctgggagt ctcctaaac gaaagtcca gatgaaatg tattgttct gacagattt aaggaagtg ttcaatttg gaccaaaac gtacgacaa cgcttcact cacgactta ttcggtcga actaccata gcttgcgga gttttctat ttgattaaa aaatacacc acgtttttg caagaatat gacgaggac cgtttcaaa gaaatcctc ctgtcgtta cttttgatc tgtagtact aacacagtg cgtgttaac gaagttagt tccactttg ggagaatac gacgtttgt ggttttgcc tgttcacat tatccgttt ttttccatg ttcaatcac tcctgctgg cccaatgtt tgtcgctct taccatggc tcccaaatg gtccttagg gccattcgt accattaaa aagggcgag cagtgtttt gtgacctat ggtcctagt tatttgtcg gataatata gtggggagg caggctttt ctgttcttt cattacttt ttcaattgc gggtgtaaa gcgtgtgtg gaaagctgg cctaagaag agtatcgta gatatgttt attaaaagt tgtcgagag caaggtgaa aactttttc tgtgctctt ctgagacgg aagttagta agagaagct gatgcggat gttgccaga aagcacatt ccttctttg ctagttaag gctaaattt gaagaattg tatcttccg cctgctgag gatttcttc gtgaatgat acattggat ttttgttac agtgtgctt gcttgcgga gttttctat ttgattaaa aaatacacc acgtttttg caagaatat gacgatggg gaccgtttc aaagaaatc ctcctgtcg ttacttttg atctgtagt actaacaca gtgcgtgtt aacgaagtt agttccaca ttgggagaa tacgacgtt tgtggtttt gcctcttca cattaccca tttttttcc atgttcaat cactcctgc tggcccaat gtttgccgc tcttaccat ggctcccaa atggtcctt agggccatt cgtaccatt aaaaagggc gagcagtgt tttgtgacc tatggtcct agttatttg tcggagaat atagtgggg aggcaggct tttctcttc tttcattac tttttcaat tgcgggtgt aaagcgtgt gtggaaagc tggcctaag aagagtatc gtagatatg tttattaaa agttgtcga gagcaaggt gaaaacttt ttctgtgct cttctgaga cggaagcta aatttgaag aattgtacc ttccgcctg ctgaggatt tcttcgtga atgatacat tggattttt gttacagtg tgcttggta ataaaagat gagtttaat t
