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Gene information
General information
TC032600
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004406 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19751612..19762303
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032600
    iBeetle screen iB_06718

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032600 RA
      MYCDKAILD PRFDVPNKI LRNLSEDPT FKASEYFDK 
FASLETNLD RINFVYQNF EKFEAYPPL ISDNKDDLL 
AGTARETGN GHFGRGEFR KALDCYTRS IQTANTKEV 
MALGFGNRS AALLKLGFY AECLTDINR ALKNEYPDK 
LKFKLLARQ NFCKGQTIV ENNAYDPVP TFPAFEKNA 
SIQCARNCI EIMQNERYG RHVVATRNI EMGEILAIE 
KPYASIVTD SVSVYCHEC LKLCYNMIP CDKCTKALY 
CSDNCKDKA YESYHKYEC PIHLSLDPL LIDSSKRLA 
LRIALISRN EWAGSLLNE SPDEMYCSD RFKEVFNLD 
QNVRQRFTH DLFGRTTIA CGVFYLIKK YTTFLQEYD 
EDRFKEILL SLLLICSTN TVRVNEVSS TLGEYDVCG 
FACSHYPFF SMFNHSCWP NVCRSYHGS QMVLRAIRT 
IKKGEQCFV TYGPSYLSD NIVGRQAFL FFHYFFNCG 
CKACVESWP KKSIVDMFI KSCREQGEN FFCALLRRK 
LVREADADV ARKHIPSLL VKAKFEELY LPPAEDFFV 
NDTLDFCYS VLACGVFYL IKKYTTFLQ EYDDGDRFK 
EILLSLLLI CSTNTVRVN EVSSTLGEY DVCGFASSH 
YPFFSMFNH SCWPNVCRS YHGSQMVLR AIRTIKKGE 
QCFVTYGPS YLSENIVGR QAFLFFHYF FNCGCKACV 
ESWPKKSIV DMFIKSCRE QGENFFCAL LRRKLNLKN 
CTFRLLRIS S
    CDS sequence
    • >TC032600 RA
      atgtattgt gataaggca atattggac ccaaggttc 
gatgtacca aataaaata cttcgaaac ttgtcggag 
gaccccact ttcaaagca tccgagtat ttcgacaag 
tttgcttca cttgaaacg aatttagat cgaataaat 
tttgtctac caaaatttc gaaaaattt gaagcatat 
cctccttta ataagtgac aataaggat gacctgctg 
gctggaaca gcaagggaa actggaaat ggacatttt 
ggacgtggc gaattcaga aaagctctg gattgttac 
acgaggagt atccaaaca gctaatact aaggaagta 
atggcactg ggttttggt aatagatca gcagcttta 
ttgaaattg ggattctac gcggagtgt ttgacggac 
ataaatcgc gcccttaaa aatgagtac cccgacaag 
ctaaaattt aaattactg gcgagacaa aatttctgc 
aaaggccaa acaatcgtg gaaaataac gcttacgac 
ccagttcca acatttccc gcttttgaa aaaaatgct 
tccattcag tgtgcaaga aactgcatt gaaatcatg 
caaaatgag cggtacgga cgccacgta gttgcaacc 
agaaacatc gaaatgggg gaaattcta gcaattgaa 
aaaccgtac gcttctatc gtaacagat tcagtttct 
gtttactgc cacgagtgt ttgaaactt tgttataac 
atgatacct tgcgataaa tgcacgaag gcattgtat 
tgcagtgat aattgcaag gacaaagct tacgaaagc 
taccacaag tacgaatgt ccgattcac ttgtccctt 
gatccttta ctaatcgac tcttcgaaa cgattggct 
ttaagaatt gcccttatt tcgagaaac gaatgggct 
gggagtctc ctaaacgaa agtccagat gaaatgtat 
tgttctgac agatttaag gaagtgttc aatttggac 
caaaacgta cgacaacgc ttcactcac gacttattc 
ggtcgaact accatagct tgcggagtt ttctatttg 
attaaaaaa tacaccacg tttttgcaa gaatatgac 
gaggaccgt ttcaaagaa atcctcctg tcgttactt 
ttgatctgt agtactaac acagtgcgt gttaacgaa 
gttagttcc actttggga gaatacgac gtttgtggt 
tttgcctgt tcacattat ccgtttttt tccatgttc 
aatcactcc tgctggccc aatgtttgt cgctcttac 
catggctcc caaatggtc cttagggcc attcgtacc 
attaaaaag ggcgagcag tgttttgtg acctatggt 
cctagttat ttgtcggat aatatagtg gggaggcag 
gcttttctg ttctttcat tactttttc aattgcggg 
tgtaaagcg tgtgtggaa agctggcct aagaagagt 
atcgtagat atgtttatt aaaagttgt cgagagcaa 
ggtgaaaac tttttctgt gctcttctg agacggaag 
ttagtaaga gaagctgat gcggatgtt gccagaaag 
cacattcct tctttgcta gttaaggct aaatttgaa 
gaattgtat cttccgcct gctgaggat ttcttcgtg 
aatgataca ttggatttt tgttacagt gtgcttgct 
tgcggagtt ttctatttg attaaaaaa tacaccacg 
tttttgcaa gaatatgac gatggggac cgtttcaaa 
gaaatcctc ctgtcgtta cttttgatc tgtagtact 
aacacagtg cgtgttaac gaagttagt tccacattg 
ggagaatac gacgtttgt ggttttgcc tcttcacat 
tacccattt ttttccatg ttcaatcac tcctgctgg 
cccaatgtt tgccgctct taccatggc tcccaaatg 
gtccttagg gccattcgt accattaaa aagggcgag 
cagtgtttt gtgacctat ggtcctagt tatttgtcg 
gagaatata gtggggagg caggctttt ctcttcttt 
cattacttt ttcaattgc gggtgtaaa gcgtgtgtg 
gaaagctgg cctaagaag agtatcgta gatatgttt 
attaaaagt tgtcgagag caaggtgaa aactttttc 
tgtgctctt ctgagacgg aagctaaat ttgaagaat 
tgtaccttc cgcctgctg aggatttct tcgtga
    mRNA sequence
    • >TC032600 RA
      cgtccagtc gggtgtttc ccccagcta cagtttgag 
acgcagttg gctttgtgc ttgtggtgt cttgatcaa 
caatagttc agcatgtat tgtgataag gcaatattg 
gacccaagg ttcgatgta ccaaataaa atacttcga 
aacttgtcg gaggacccc actttcaaa gcatccgag 
tatttcgac aagtttgct tcacttgaa acgaattta 
gatcgaata aattttgtc taccaaaat ttcgaaaaa 
tttgaagca tatcctcct ttaataagt gacaataag 
gatgacctg ctggctgga acagcaagg gaaactgga 
aatggacat tttggacgt ggcgaattc agaaaagct 
ctggattgt tacacgagg agtatccaa acagctaat 
actaaggaa gtaatggca ctgggtttt ggtaataga 
tcagcagct ttattgaaa ttgggattc tacgcggag 
tgtttgacg gacataaat cgcgccctt aaaaatgag 
taccccgac aagctaaaa tttaaatta ctggcgaga 
caaaatttc tgcaaaggc caaacaatc gtggaaaat 
aacgcttac gacccagtt ccaacattt cccgctttt 
gaaaaaaat gcttccatt cagtgtgca agaaactgc 
attgaaatc atgcaaaat gagcggtac ggacgccac 
gtagttgca accagaaac atcgaaatg ggggaaatt 
ctagcaatt gaaaaaccg tacgcttct atcgtaaca 
gattcagtt tctgtttac tgccacgag tgtttgaaa 
ctttgttat aacatgata ccttgcgat aaatgcacg 
aaggcattg tattgcagt gataattgc aaggacaaa 
gcttacgaa agctaccac aagtacgaa tgtccgatt 
cacttgtcc cttgatcct ttactaatc gactcttcg 
aaacgattg gctttaaga attgccctt atttcgaga 
aacgaatgg gctgggagt ctcctaaac gaaagtcca 
gatgaaatg tattgttct gacagattt aaggaagtg 
ttcaatttg gaccaaaac gtacgacaa cgcttcact 
cacgactta ttcggtcga actaccata gcttgcgga 
gttttctat ttgattaaa aaatacacc acgtttttg 
caagaatat gacgaggac cgtttcaaa gaaatcctc 
ctgtcgtta cttttgatc tgtagtact aacacagtg 
cgtgttaac gaagttagt tccactttg ggagaatac 
gacgtttgt ggttttgcc tgttcacat tatccgttt 
ttttccatg ttcaatcac tcctgctgg cccaatgtt 
tgtcgctct taccatggc tcccaaatg gtccttagg 
gccattcgt accattaaa aagggcgag cagtgtttt 
gtgacctat ggtcctagt tatttgtcg gataatata 
gtggggagg caggctttt ctgttcttt cattacttt 
ttcaattgc gggtgtaaa gcgtgtgtg gaaagctgg 
cctaagaag agtatcgta gatatgttt attaaaagt 
tgtcgagag caaggtgaa aactttttc tgtgctctt 
ctgagacgg aagttagta agagaagct gatgcggat 
gttgccaga aagcacatt ccttctttg ctagttaag 
gctaaattt gaagaattg tatcttccg cctgctgag 
gatttcttc gtgaatgat acattggat ttttgttac 
agtgtgctt gcttgcgga gttttctat ttgattaaa 
aaatacacc acgtttttg caagaatat gacgatggg 
gaccgtttc aaagaaatc ctcctgtcg ttacttttg 
atctgtagt actaacaca gtgcgtgtt aacgaagtt 
agttccaca ttgggagaa tacgacgtt tgtggtttt 
gcctcttca cattaccca tttttttcc atgttcaat 
cactcctgc tggcccaat gtttgccgc tcttaccat 
ggctcccaa atggtcctt agggccatt cgtaccatt 
aaaaagggc gagcagtgt tttgtgacc tatggtcct 
agttatttg tcggagaat atagtgggg aggcaggct 
tttctcttc tttcattac tttttcaat tgcgggtgt 
aaagcgtgt gtggaaagc tggcctaag aagagtatc 
gtagatatg tttattaaa agttgtcga gagcaaggt 
gaaaacttt ttctgtgct cttctgaga cggaagcta 
aatttgaag aattgtacc ttccgcctg ctgaggatt 
tcttcgtga atgatacat tggattttt gttacagtg 
tgcttggta ataaaagat gagtttaat t
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