Status
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Gene information
General information
TC032600
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004406
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19751612..19762303
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032600
    iBeetle screen iB_06718

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032600 RA
      MYCDKAILD PRFDVPNKI LRNLSEDPT FKASEYFDK 
      FASLETNLD RINFVYQNF EKFEAYPPL ISDNKDDLL 
      AGTARETGN GHFGRGEFR KALDCYTRS IQTANTKEV 
      MALGFGNRS AALLKLGFY AECLTDINR ALKNEYPDK 
      LKFKLLARQ NFCKGQTIV ENNAYDPVP TFPAFEKNA 
      SIQCARNCI EIMQNERYG RHVVATRNI EMGEILAIE 
      KPYASIVTD SVSVYCHEC LKLCYNMIP CDKCTKALY 
      CSDNCKDKA YESYHKYEC PIHLSLDPL LIDSSKRLA 
      LRIALISRN EWAGSLLNE SPDEMYCSD RFKEVFNLD 
      QNVRQRFTH DLFGRTTIA CGVFYLIKK YTTFLQEYD 
      EDRFKEILL SLLLICSTN TVRVNEVSS TLGEYDVCG 
      FACSHYPFF SMFNHSCWP NVCRSYHGS QMVLRAIRT 
      IKKGEQCFV TYGPSYLSD NIVGRQAFL FFHYFFNCG 
      CKACVESWP KKSIVDMFI KSCREQGEN FFCALLRRK 
      LVREADADV ARKHIPSLL VKAKFEELY LPPAEDFFV 
      NDTLDFCYS VLACGVFYL IKKYTTFLQ EYDDGDRFK 
      EILLSLLLI CSTNTVRVN EVSSTLGEY DVCGFASSH 
      YPFFSMFNH SCWPNVCRS YHGSQMVLR AIRTIKKGE 
      QCFVTYGPS YLSENIVGR QAFLFFHYF FNCGCKACV 
      ESWPKKSIV DMFIKSCRE QGENFFCAL LRRKLNLKN 
      CTFRLLRIS S
    CDS sequence
    • >TC032600 RA
      atgtattgt gataaggca atattggac ccaaggttc 
      gatgtacca aataaaata cttcgaaac ttgtcggag 
      gaccccact ttcaaagca tccgagtat ttcgacaag 
      tttgcttca cttgaaacg aatttagat cgaataaat 
      tttgtctac caaaatttc gaaaaattt gaagcatat 
      cctccttta ataagtgac aataaggat gacctgctg 
      gctggaaca gcaagggaa actggaaat ggacatttt 
      ggacgtggc gaattcaga aaagctctg gattgttac 
      acgaggagt atccaaaca gctaatact aaggaagta 
      atggcactg ggttttggt aatagatca gcagcttta 
      ttgaaattg ggattctac gcggagtgt ttgacggac 
      ataaatcgc gcccttaaa aatgagtac cccgacaag 
      ctaaaattt aaattactg gcgagacaa aatttctgc 
      aaaggccaa acaatcgtg gaaaataac gcttacgac 
      ccagttcca acatttccc gcttttgaa aaaaatgct 
      tccattcag tgtgcaaga aactgcatt gaaatcatg 
      caaaatgag cggtacgga cgccacgta gttgcaacc 
      agaaacatc gaaatgggg gaaattcta gcaattgaa 
      aaaccgtac gcttctatc gtaacagat tcagtttct 
      gtttactgc cacgagtgt ttgaaactt tgttataac 
      atgatacct tgcgataaa tgcacgaag gcattgtat 
      tgcagtgat aattgcaag gacaaagct tacgaaagc 
      taccacaag tacgaatgt ccgattcac ttgtccctt 
      gatccttta ctaatcgac tcttcgaaa cgattggct 
      ttaagaatt gcccttatt tcgagaaac gaatgggct 
      gggagtctc ctaaacgaa agtccagat gaaatgtat 
      tgttctgac agatttaag gaagtgttc aatttggac 
      caaaacgta cgacaacgc ttcactcac gacttattc 
      ggtcgaact accatagct tgcggagtt ttctatttg 
      attaaaaaa tacaccacg tttttgcaa gaatatgac 
      gaggaccgt ttcaaagaa atcctcctg tcgttactt 
      ttgatctgt agtactaac acagtgcgt gttaacgaa 
      gttagttcc actttggga gaatacgac gtttgtggt 
      tttgcctgt tcacattat ccgtttttt tccatgttc 
      aatcactcc tgctggccc aatgtttgt cgctcttac 
      catggctcc caaatggtc cttagggcc attcgtacc 
      attaaaaag ggcgagcag tgttttgtg acctatggt 
      cctagttat ttgtcggat aatatagtg gggaggcag 
      gcttttctg ttctttcat tactttttc aattgcggg 
      tgtaaagcg tgtgtggaa agctggcct aagaagagt 
      atcgtagat atgtttatt aaaagttgt cgagagcaa 
      ggtgaaaac tttttctgt gctcttctg agacggaag 
      ttagtaaga gaagctgat gcggatgtt gccagaaag 
      cacattcct tctttgcta gttaaggct aaatttgaa 
      gaattgtat cttccgcct gctgaggat ttcttcgtg 
      aatgataca ttggatttt tgttacagt gtgcttgct 
      tgcggagtt ttctatttg attaaaaaa tacaccacg 
      tttttgcaa gaatatgac gatggggac cgtttcaaa 
      gaaatcctc ctgtcgtta cttttgatc tgtagtact 
      aacacagtg cgtgttaac gaagttagt tccacattg 
      ggagaatac gacgtttgt ggttttgcc tcttcacat 
      tacccattt ttttccatg ttcaatcac tcctgctgg 
      cccaatgtt tgccgctct taccatggc tcccaaatg 
      gtccttagg gccattcgt accattaaa aagggcgag 
      cagtgtttt gtgacctat ggtcctagt tatttgtcg 
      gagaatata gtggggagg caggctttt ctcttcttt 
      cattacttt ttcaattgc gggtgtaaa gcgtgtgtg 
      gaaagctgg cctaagaag agtatcgta gatatgttt 
      attaaaagt tgtcgagag caaggtgaa aactttttc 
      tgtgctctt ctgagacgg aagctaaat ttgaagaat 
      tgtaccttc cgcctgctg aggatttct tcgtga
    mRNA sequence
    • >TC032600 RA
      cgtccagtc gggtgtttc ccccagcta cagtttgag 
      acgcagttg gctttgtgc ttgtggtgt cttgatcaa 
      caatagttc agcatgtat tgtgataag gcaatattg 
      gacccaagg ttcgatgta ccaaataaa atacttcga 
      aacttgtcg gaggacccc actttcaaa gcatccgag 
      tatttcgac aagtttgct tcacttgaa acgaattta 
      gatcgaata aattttgtc taccaaaat ttcgaaaaa 
      tttgaagca tatcctcct ttaataagt gacaataag 
      gatgacctg ctggctgga acagcaagg gaaactgga 
      aatggacat tttggacgt ggcgaattc agaaaagct 
      ctggattgt tacacgagg agtatccaa acagctaat 
      actaaggaa gtaatggca ctgggtttt ggtaataga 
      tcagcagct ttattgaaa ttgggattc tacgcggag 
      tgtttgacg gacataaat cgcgccctt aaaaatgag 
      taccccgac aagctaaaa tttaaatta ctggcgaga 
      caaaatttc tgcaaaggc caaacaatc gtggaaaat 
      aacgcttac gacccagtt ccaacattt cccgctttt 
      gaaaaaaat gcttccatt cagtgtgca agaaactgc 
      attgaaatc atgcaaaat gagcggtac ggacgccac 
      gtagttgca accagaaac atcgaaatg ggggaaatt 
      ctagcaatt gaaaaaccg tacgcttct atcgtaaca 
      gattcagtt tctgtttac tgccacgag tgtttgaaa 
      ctttgttat aacatgata ccttgcgat aaatgcacg 
      aaggcattg tattgcagt gataattgc aaggacaaa 
      gcttacgaa agctaccac aagtacgaa tgtccgatt 
      cacttgtcc cttgatcct ttactaatc gactcttcg 
      aaacgattg gctttaaga attgccctt atttcgaga 
      aacgaatgg gctgggagt ctcctaaac gaaagtcca 
      gatgaaatg tattgttct gacagattt aaggaagtg 
      ttcaatttg gaccaaaac gtacgacaa cgcttcact 
      cacgactta ttcggtcga actaccata gcttgcgga 
      gttttctat ttgattaaa aaatacacc acgtttttg 
      caagaatat gacgaggac cgtttcaaa gaaatcctc 
      ctgtcgtta cttttgatc tgtagtact aacacagtg 
      cgtgttaac gaagttagt tccactttg ggagaatac 
      gacgtttgt ggttttgcc tgttcacat tatccgttt 
      ttttccatg ttcaatcac tcctgctgg cccaatgtt 
      tgtcgctct taccatggc tcccaaatg gtccttagg 
      gccattcgt accattaaa aagggcgag cagtgtttt 
      gtgacctat ggtcctagt tatttgtcg gataatata 
      gtggggagg caggctttt ctgttcttt cattacttt 
      ttcaattgc gggtgtaaa gcgtgtgtg gaaagctgg 
      cctaagaag agtatcgta gatatgttt attaaaagt 
      tgtcgagag caaggtgaa aactttttc tgtgctctt 
      ctgagacgg aagttagta agagaagct gatgcggat 
      gttgccaga aagcacatt ccttctttg ctagttaag 
      gctaaattt gaagaattg tatcttccg cctgctgag 
      gatttcttc gtgaatgat acattggat ttttgttac 
      agtgtgctt gcttgcgga gttttctat ttgattaaa 
      aaatacacc acgtttttg caagaatat gacgatggg 
      gaccgtttc aaagaaatc ctcctgtcg ttacttttg 
      atctgtagt actaacaca gtgcgtgtt aacgaagtt 
      agttccaca ttgggagaa tacgacgtt tgtggtttt 
      gcctcttca cattaccca tttttttcc atgttcaat 
      cactcctgc tggcccaat gtttgccgc tcttaccat 
      ggctcccaa atggtcctt agggccatt cgtaccatt 
      aaaaagggc gagcagtgt tttgtgacc tatggtcct 
      agttatttg tcggagaat atagtgggg aggcaggct 
      tttctcttc tttcattac tttttcaat tgcgggtgt 
      aaagcgtgt gtggaaagc tggcctaag aagagtatc 
      gtagatatg tttattaaa agttgtcga gagcaaggt 
      gaaaacttt ttctgtgct cttctgaga cggaagcta 
      aatttgaag aattgtacc ttccgcctg ctgaggatt 
      tcttcgtga atgatacat tggattttt gttacagtg 
      tgcttggta ataaaagat gagtttaat t
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