Status
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Gene information
General information
TC031805
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010726
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 497848..504572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031805
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06716

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031805 RA
      MYLKRRIYK QITSIDFLE PFLKRLKFN KDFCNILQS 
      PQQIFYNFV NIVLLTHGK AFVLHQTAY NIINLYEKF 
      DVANKIHQS RAEFIRDIN NIIRMLIKN NVENISREL 
      WRCNPDKHI RGKTYLEIT NFLHGYIIN KKNLDPKKQ 
      CVNECDSFK MTAIQNCDD DIDEPWCFK ESPCSRIIS 
      CTYIDSSMD VCLANVPRD NRRYHHIEL GNGTFFGTN 
      QQCTRPKIN LATYSESLI FKCSYCFCL CDEGQGSYS 
      DRYINLRIS MSNISDNRV VTGLRFIKH NRIVHLQVQ 
      EGKLLEKGN INPTTVQWV PPEDYKITD RDIYKGQDY 
      HTISWEERT IELTKVIAK AGSVVTGVR FKRTFGSLN 
      LQVLTTAFD FNTGKLEKN SDSDWGIFE SAPYFNIRH 
      KFRLKVNLN PSDVPVRTN LDFTDFSEN MYIEFANTD 
      YRKDAGQTT IPFFDAQAV NSTTPTPLS GIGLFHKGR 
      KGYGGFIAP KIITYDFSR HILNQIDP
    CDS sequence
    • >TC031805 RA
      atgtatttg aagcgaaga atatacaaa caaataaca 
      agtatagat tttttggaa ccgtttctg aaaagattg 
      aagttcaat aaggatttc tgtaatata ctacaatct 
      ccacaacag atattttac aattttgtt aatattgtt 
      ctactaaca catggcaaa gcatttgtt ctacaccag 
      acagcttac aatatcata aacctctat gaaaaattt 
      gatgttgct aataagatt catcaaagt cgagctgaa 
      tttatcaga gacatcaac aatataata agaatgcta 
      ataaagaat aatgttgaa aatatatcc agagaatta 
      tggagatgt aaccccgac aagcatatt agaggcaaa 
      acttacttg gaaatcaca aattttctt catggctac 
      ataataaac aaaaaaaat ttggaccca aaaaaacaa 
      tgcgtgaac gagtgtgac tcttttaaa atgacagca 
      attcaaaat tgtgatgat gatattgat gaaccatgg 
      tgttttaaa gaaagtcct tgttcaagg atcattagt 
      tgtacatat attgattca tcaatggac gtgtgtctt 
      gctaacgtc cctagagat aacagaaga tatcaccac 
      attgaacta ggaaacgga acatttttt ggaacaaac 
      caacaatgc actcgtcca aagataaat cttgcaact 
      tacagtgaa tcgttaatt ttcaaatgt tcttattgt 
      ttctgtttg tgcgacgaa ggacaggga tcctattct 
      gacagatat attaattta cgtattagt atgtccaac 
      ataagtgac aacagagtt gtcaccggc ttaaggttc 
      attaagcac aatcggatt gttcatttg caagttcaa 
      gaaggtaaa cttcttgaa aaagggaat attaatcca 
      acaactgtt cagtgggtt ccccccgaa gattataaa 
      atcactgat agagacatt tataaagga caagattac 
      catacaatt tcttgggaa gaacgaact atagagtta 
      actaaagtt atagccaaa gctggatca gttgtaaca 
      ggggttaga ttcaaaaga acttttggt agtttaaac 
      ttacaagtt ctgacaact gcattcgat ttcaacact 
      ggaaaatta gaaaaaaat tcagattca gattggggt 
      atttttgaa agcgcacca tatttcaat attaggcac 
      aaattcagg ctaaaagtg aatttaaac ccatcggac 
      gttcctgtt cgcacaaat ttggatttt acggatttt 
      tcagaaaat atgtatatt gaatttgca aatacagac 
      tatcgcaaa gacgccggt caaacgaca ataccattc 
      ttcgacgct caggctgtt aacagtact actccaact 
      ccattgtca ggtatcggt ctctttcac aaaggacga 
      aaaggatat ggaggtttt atagcacca aaaattata 
      acttacgat tttagtaga catatactt aaccaaata 
      gatccctag
    mRNA sequence
    • >TC031805 RA
      tttgaagaa acaaatatg attgccaac gcttttctg 
      tgaatggcc cttttccgc ttaaaaagt gaaaaacta 
      caccctgtt tacgacctc atcgctcag tcagtttaa 
      actactata ctttttttc tgtctgttt gctagtgat 
      agagataaa catatttat cagttaggt atccaaaag 
      atacccgta aattcgtaa ttctgcagt gtgaaaatt 
      gcatttcat aatgcaatt tttatatgt gttctcaat 
      ttatgtatt catcttacc acccataaa atgtactgt 
      acgaaacaa tccttctct actaattta cgaattcgt 
      tgaaataac aaagaacct aactaatct atttagaat 
      tatcaacga tagtaatta tataacaca tgatgacaa 
      agaagaaca tgttttcac aacactaga cgttattaa 
      caataagat tagagggct gaaccgatc actttatgg 
      agatgaact gactgcgaa tgtcattgc aagggattt 
      acctatcgt cgacataaa aaccataga cactcattt 
      tagtgaaat ggaaaattt tgtcagaca ttataaaca 
      atttccaaa atcaatttt tttggataa tgcctacag 
      aataatttc accaaatgc aaactcatc agttatgta 
      tttgaagcg aagaatata caaacaaat aacaagtat 
      agatttttt ggaaccgtt tctgaaaag attgaagtt 
      caataagga tttctgtaa tatactaca atctccaca 
      acagatatt ttacaattt tgttaatat tgttctact 
      aacacatgg caaagcatt tgttctaca ccagacagc 
      ttacaatat cataaacct ctatgaaaa atttgatgt 
      tgctaataa gattcatca aagtcgagc tgaatttat 
      cagagacat caacaatat aataagaat gctaataaa 
      gaataatgt tgaaaatat atccagaga attatggag 
      atgtaaccc cgacaagca tattagagg caaaactta 
      cttggaaat cacaaattt tcttcatgg ctacataat 
      aaacaaaaa aaatttgga cccaaaaaa acaatgcgt 
      gaacgagtg tgactcttt taaaatgac agcaattca 
      aaattgtga tgatgatat tgatgaacc atggtgttt 
      taaagaaag tccttgttc aaggatcat tagttgtac 
      atatattga ttcatcaat ggacgtgtg tcttgctaa 
      cgtccctag agataacag aagatatca ccacattga 
      actaggaaa cggaacatt ttttggaac aaaccaaca 
      atgcactcg tccaaagat aaatcttgc aacttacag 
      tgaatcgtt aattttcaa atgttctta ttgtttctg 
      tttgtgcga cgaaggaca gggatccta ttctgacag 
      atatattaa tttacgtat tagtatgtc caacataag 
      tgacaacag agttgtcac cggcttaag gttcattaa 
      gcacaatcg gattgttca tttgcaagt tcaagaagg 
      taaacttct tgaaaaagg gaatattaa tccaacaac 
      tgttcagtg ggttccccc cgaagatta taaaatcac 
      tgatagaga catttataa aggacaaga ttaccatac 
      aatttcttg ggaagaacg aactataga gttaactaa 
      agttatagc caaagctgg atcagttgt aacaggggt 
      tagattcaa aagaacttt tggtagttt aaacttaca 
      agttctgac aactgcatt cgatttcaa cactggaaa 
      attagaaaa aaattcaga ttcagattg gggtatttt 
      tgaaagcgc accatattt caatattag gcacaaatt 
      caggctaaa agtgaattt aaacccatc ggacgttcc 
      tgttcgcac aaatttgga ttttacgga tttttcaga 
      aaatatgta tattgaatt tgcaaatac agactatcg 
      caaagacgc cggtcaaac gacaatacc attcttcga 
      cgctcaggc tgttaacag tactactcc aactccatt 
      gtcaggtat cggtctctt tcacaaagg acgaaaagg 
      atatggagg ttttatagc accaaaaat tataactta 
      cgattttag tagacatat acttaacca aatagatcc 
      ctagaacag tgttgttaa ttaatgtaa ttatacaga 
      gtaagtttt aactttaca ttttaaata atcccaaga 
      ttggcacta ttttgacta aacttggta tactggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY