Status
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Gene information
General information
TC031879
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006938
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 309373..315463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031879
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06712

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04240

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031879 RA
      MQAKPSTEP SSTPTSTTA SEQHRRFAS SVPTHEATG 
      TKPPTSYLT TIMHLAKCY VGTGIFAMG EGFKNSGLI 
      LGPVLLAFL ALLNLNCQH ILVKTVIKI ADEEVEDVK 
      PTFAETVEY TFEGSSINC FKRNSKALA WMTNIFLCC 
      TELGFCCVY FVFIAEHLV KIAEHNNLM TENHPGNKH 
      IMLLIILPP MWASTFLGN LKLLLPLSI IANILMWAG 
      VIIIVYFTV QNLDASAWT KNAVNSVHR WPLFFGTAL 
      YAFEGITFV IPLRNEMKQ PEKFLSAFG VLNVGMTFV 
      AFLYILVGL LAYWKYGDN VASSVFLNI TADSKLLPD 
      IINAMMAVA VLFTFTLHM YVPFEITFP LFYRKYGPF 
      KHTRLVAII YRSIPVLLT FTMANVIPF LGLFISLVE 
      LGHKFGRPD IERIGLFSE MVYLSAKTF PKKKTQREP 
      RNMYCEGRK SKNGTQHGY FDPEFKRVF SKEAIKGRG 
      RKPAPPKFK NVSERPFLP PIGFKHHSC PGDYHGTFS 
      GIIEAFGNK LKPRPKRKS EGRNLLSNP GKLGGPGYA 
      DICLSRYPE HKPDRYGLK PKYKEYGKN LGRAMVPVH 
      FPQPFFDKN PFADPPNIK PGPTYVRPK DKELKSIHP 
      GYIVPTGPG KWQGGCHDG CFDKFPEYK PDKYGVRVK 
      KPHEKIFYP PSTALKSYY NTSILFQNV DVKLNKVNY 
      KNFQPAYNK YIIIND
    CDS sequence
    • >TC031879 RA
      atgcaagct aaaccatcc actgaacct tcttcaact 
      ccaacttca acaacagct tccgaacaa catcgcaga 
      tttgccagt tcggttcct acacatgaa gctacagga 
      accaaacca ccaactagt tacttaacc acaataatg 
      catcttgct aaatgttac gttggcacg ggaattttt 
      gcaatggga gaaggcttc aaaaacagt ggacttata 
      ctaggccct gtcctactt gctttcctg gctctttta 
      aatctaaat tgccaacac attttagtg aaaactgtt 
      ataaaaatc gccgatgaa gaagtggaa gatgtgaaa 
      ccaactttt gctgaaacc gttgaatac acttttgag 
      ggcagttct ataaactgt ttcaaaaga aactcgaaa 
      gctttggct tggatgaca aacattttt ttgtgttgt 
      actgaactg ggattttgc tgtgtttac ttcgtattc 
      attgcagaa cacttagtt aaaatagcc gaacataat 
      aacttgatg acggaaaac caccctgga aacaagcac 
      ataatgctc ttaattatt ttaccccca atgtgggct 
      tccacattt ttgggcaat ttgaaatta ttgctccct 
      ttatcaatc attgcaaat attttaatg tgggctggg 
      gttataata atagtttac tttactgtg caaaatctt 
      gatgctagt gcttggaca aaaaatgcc gtaaattct 
      gtacatcga tggccgttg ttttttgga actgcactt 
      tacgctttt gaaggaata acatttgtc attccgtta 
      cgaaatgaa atgaaacag ccggaaaaa ttcctgagt 
      gctttcggt gtattaaat gtgggaatg acgtttgtg 
      gcatttttg tacatcttg gttggctta ctggcttac 
      tggaagtat ggagataat gttgcaagt agcgttttt 
      ttaaatatc actgccgat agcaaatta ttgccggat 
      attatcaat gcaatgatg gcagtggca gtgttattt 
      acttttact cttcatatg tatgttccg tttgaaatt 
      acttttccg ttgttttat cgaaaatat ggacctttt 
      aaacacaca cgacttgtg gcgatcatt tacagatca 
      attccagtg ttgctgaca tttacaatg gcaaacgta 
      attccattt ttgggcctc ttcatatca cttgtggaa 
      cttggccac aaattcgga agacctgat attgaaaga 
      atcggattg ttcagtgaa atggtgtat ttaagtgca 
      aaaacgttc ccaaaaaag aaaactcaa cgggaacca 
      agaaatatg tactgtgag ggccgaaaa tctaaaaat 
      gggacacaa catggatac tttgaccct gaatttaaa 
      agagttttt tcaaaagaa gcgataaaa ggcagaggc 
      agaaaaccg gcgcctcca aaatttaaa aacgtttca 
      gagcgtcct tttttaccc ccaataggt ttcaaacac 
      cattcatgt cctggtgat taccacgga acattcagt 
      ggaattatt gaagctttt ggtaataaa ttaaaaccg 
      agacctaag cgaaaatca gaaggaaga aacttgttg 
      tccaaccct ggaaaatta ggaggtcct ggatatgcc 
      gacatatgt ctgagtcgt tatccagaa cacaagcct 
      gataggtat ggtttaaaa ccaaagtac aaagaatat 
      gggaaaaat ttaggaagg gctatggtc ccagttcac 
      ttccctcaa ccgtttttt gacaaaaat ccatttgct 
      gacccgcca aacatcaaa cctggacct acttacgtc 
      agaccaaaa gataaagaa ttgaaaagt attcatcct 
      ggttatatt gtaccaact gggccgggc aaatggcaa 
      ggaggatgt catgatgga tgtttcgat aaatttcca 
      gaatacaaa ccggataaa tatggggta cgagttaag 
      aaacctcat gaaaaaatt ttttatcct ccatcaact 
      gcactcaag agctattac aatacatcc attttgttc 
      caaaacgtt gatgttaaa cttaacaaa gtaaactat 
      aaaaatttc cagccggct tataataaa tacataata 
      attaatgat tag
    mRNA sequence
    • >TC031879 RA
      aagccacaa atttgtcat ttttgacat gtaaattac 
      ttttacaaa tctttacct aaatttact tttacattt 
      acaataatg caagctaaa ccatccact gaaccttct 
      tcaactcca acttcaaca acagcttcc gaacaacat 
      cgcagattt gccagttcg gttcctaca catgaagct 
      acaggaacc aaaccacca actagttac ttaaccaca 
      ataatgcat cttgctaaa tgttacgtt ggcacggga 
      atttttgca atgggagaa ggcttcaaa aacagtgga 
      cttatacta ggccctgtc ctacttgct ttcctggct 
      cttttaaat ctaaattgc caacacatt ttagtgaaa 
      actgttata aaaatcgcc gatgaagaa gtggaagat 
      gtgaaacca acttttgct gaaaccgtt gaatacact 
      tttgagggc agttctata aactgtttc aaaagaaac 
      tcgaaagct ttggcttgg atgacaaac atttttttg 
      tgttgtact gaactggga ttttgctgt gtttacttc 
      gtattcatt gcagaacac ttagttaaa atagccgaa 
      cataataac ttgatgacg gaaaaccac cctggaaac 
      aagcacata atgctctta attatttta cccccaatg 
      tgggcttcc acatttttg ggcaatttg aaattattg 
      ctcccttta tcaatcatt gcaaatatt ttaatgtgg 
      gctggggtt ataataata gtttacttt actgtgcaa 
      aatcttgat gctagtgct tggacaaaa aatgccgta 
      aattctgta catcgatgg ccgttgttt tttggaact 
      gcactttac gcttttgaa ggaataaca tttgtcatt 
      ccgttacga aatgaaatg aaacagccg gaaaaattc 
      ctgagtgct ttcggtgta ttaaatgtg ggaatgacg 
      tttgtggca tttttgtac atcttggtt ggcttactg 
      gcttactgg aagtatgga gataatgtt gcaagtagc 
      gttttttta aatatcact gccgatagc aaattattg 
      ccggatatt atcaatgca atgatggca gtggcagtg 
      ttatttact tttactctt catatgtat gttccgttt 
      gaaattact tttccgttg ttttatcga aaatatgga 
      ccttttaaa cacacacga cttgtggcg atcatttac 
      agatcaatt ccagtgttg ctgacattt acaatggca 
      aacgtaatt ccatttttg ggcctcttc atatcactt 
      gtggaactt ggccacaaa ttcggaaga cctgatatt 
      gaaagaatc ggattgttc agtgaaatg gtgtattta 
      agtgcaaaa acgttccca aaaaagaaa actcaacgg 
      gaaccaaga aatatgtac tgtgagggc cgaaaatct 
      aaaaatggg acacaacat ggatacttt gaccctgaa 
      tttaaaaga gttttttca aaagaagcg ataaaaggc 
      agaggcaga aaaccggcg cctccaaaa tttaaaaac 
      gtttcagag cgtcctttt ttaccccca ataggtttc 
      aaacaccat tcatgtcct ggtgattac cacggaaca 
      ttcagtgga attattgaa gcttttggt aataaatta 
      aaaccgaga cctaagcga aaatcagaa ggaagaaac 
      ttgttgtcc aaccctgga aaattagga ggtcctgga 
      tatgccgac atatgtctg agtcgttat ccagaacac 
      aagcctgat aggtatggt ttaaaacca aagtacaaa 
      gaatatggg aaaaattta ggaagggct atggtccca 
      gttcacttc cctcaaccg ttttttgac aaaaatcca 
      tttgctgac ccgccaaac atcaaacct ggacctact 
      tacgtcaga ccaaaagat aaagaattg aaaagtatt 
      catcctggt tatattgta ccaactggg ccgggcaaa 
      tggcaagga ggatgtcat gatggatgt ttcgataaa 
      tttccagaa tacaaaccg gataaatat ggggtacga 
      gttaagaaa cctcatgaa aaaattttt tatcctcca 
      tcaactgca ctcaagagc tattacaat acatccatt 
      ttgttccaa aacgttgat gttaaactt aacaaagta 
      aactataaa aatttccag ccggcttat aataaatac 
      ataataatt aatgattag aaaaagtgt ttgttttaa 
      tttttatat aaaataaat cgcattacg taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY