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Gene information
General information
TC001863 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 558079..559220
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC001863
      iBeetle screen iB_06710

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      low validity due to technical reasons - should be repeated: musculature not scorable,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head segment random number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs segment shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001863 RA
        MENVFASHS DLLDSCFEE LDYEYSDEE 
IDDLDDSFT SESGICTQS ASPSSIHLD 
ESENLPFKL RRELIKFDK VPSKLLDKR 
KWKTPKAGY IKLWRIRSS DGCFDMRRR 
CSTDSGGQN ERVVKNICS LKAVIGSNL 
PSRTSDTKN RAYKESPES IDASISITF 
FNDLSCKKS PNVGK
      CDS sequence
      • >TC001863 RA
        atggagaat gtgtttgca agtcactcc gatctcttg 
gactcctgc tttgaggaa ctggactat gagtacagt 
gacgaagaa attgacgat ttagatgat tctttcacg 
tctgaaagt ggaatttgt acgcagtcc gcctccccg 
tcttcaata catttggat gaaagtgaa aatttgccg 
ttcaaactg agaagagaa ttaataaag tttgataaa 
gtgccatca aaactgttg gacaaacgc aaatggaaa 
acccctaag gcgggttat ataaaactg tggcggatt 
aggtcgtca gatggttgt tttgatatg aggagacga 
tgttcgact gattctgga ggacaaaat gaacgtgtt 
gtaaaaaat atttgttca ctcaaagcc gtaattgga 
tcaaattta ccctctagg actagtgat accaaaaac 
agggcgtat aaagaatca cctgagagt attgacgcg 
tccattagc atcactttc tttaatgat ttgagttgc 
aaaaaaagt cccaacgtt ggtaagtaa
      mRNA sequence
      • >TC001863 RA
        ccataaatt aatatgtag ttgtttttt gaaatttaa 
atgtgtttt tatctaatt atcataaat taaattttt 
ttctatttt ttaggttgg cgctatttt ggcacgcat 
gcgtgtttg tttaataaa ttggccact gtagcagtt 
tgcagttag gttagggat gtttttaat gttgcaaat 
gtataaaaa agagcaaaa actgctttt atttatcac 
caatgtgat atgtaagtt tccgatata aaaccaata 
aatggagaa tgtgtttgc aagtcactc cgatctctt 
ggactcctg ctttgagga actggacta tgagtacag 
tgacgaaga aattgacga tttagatga ttctttcac 
gtctgaaag tggaatttg tacgcagtc cgcctcccc 
gtcttcaat acatttgga tgaaagtga aaatttgcc 
gttcaaact gagaagaga attaataaa gtttgataa 
agtgccatc aaaactgtt ggacaaacg caaatggaa 
aacccctaa ggcgggtta tataaaact gtggcggat 
taggtcgtc agatggttg ttttgatat gaggagacg 
atgttcgac tgattctgg aggacaaaa tgaacgtgt 
tgtaaaaaa tatttgttc actcaaagc cgtaattgg 
atcaaattt accctctag gactagtga taccaaaaa 
cagggcgta taaagaatc acctgagag tattgacgc 
gtccattag catcacttt ctttaatga tttgagttg 
caaaaaaag tcccaacgt tggtaagta atccctatg 
actgatgta ttacctaag tgacatcac taatttgta 
tatattttg ttaagattt gtacgttta ttaatactg 
gtaatgatt gttttgttg tgatttatt tatagtata 
tattaaagt cagaagttg gctgcttaa gaggaactt 
ttcaacttt aacgttagt attcttgtt attttttat 
attttttca ataaacttc ctaggtata a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY