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Gene information
General information
TC001864 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 531953..541688
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001864
      iBeetle screen iB_06709

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      larva segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax potentially partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs podomer sometimes fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001864 RA
        MLKPEFSCI VAFFWAFWP RSSERKRFS VEEAPELSR 
        FRMRKWFER VVKPKIRCS RFGLLVSGL CVVSSAILG 
        ILPLVRPGV TLRCDLRNQ GGRTALCTV EDTFDGTCY 
        IICRDSCGI MGNIGSQTG QITQRPLPE EQQWAHSFP 
        RTQPRLHQH KVLPERDAR QKLRHTNNG EILQSGGTL 
        SGRHHQNRL SLRENSNEF STLDKPRTY LSNQRDPLH 
        KSGARYTHA MEYKHFSHS AYRENMKKF GSEPDLRHS 
        KGMETQDRR GKKKYKAPP PPVEVNKSL DWDDSSGDN 
        GPTRRARLF KTRAETRKN YSSPVHNHK SLEKHEELV 
        EIRDRDRSA DLRTNRLSL PEMITPNFH QELKEVTKR 
        LRHTRASTD NTIAEINNR NKEKLKTLD HNIHKKDNI 
        RQRLKQMET VEEKLDPVR GDGSGKESS SPDQSSPII 
        RKKEQPKTF YFGMDETMQ NEIVDHFAS NLHSMTHIP 
        KIANSSESD ISSEIEADE NTGVRNGID LQLRPILPK 
        KQLEIPRFS PAAAWRLLS VDTNPTAST IASDEVPVF 
        IEDRIEKYS RPPPPTMQI GQRSNNDKS GDSGISGDA 
        GPPGYDDSP DAIMNSRNS LLIPKGISW TPQQDLCDD 
        SSMEEGLNN ENSYKPRSI YSNKPHVFS LSLRGDNHL 
        SAYMSDKTY TGLQKLKRD NWFLSKSAP NSLNNGFNS 
        LEMKNSNKS EEKEQLLVP NSKSSGRLM YLPEFDNNQ 
        DHRHARAKT KDDLRERSK SADRSKTNH KLSVFSKSC 
        ENINGELKS SSDAEPEDL QDPPKVFED ARNWNRKPR 
        RFTFQSTVR QIERRRLAE KLSREAEKK EKQRLRELE 
        AMQKVEEEF QKKRAREKA NIRQQLRLF SMDDVAWTS 
        LPPNLEVKD EVRQEPDGA VSSSTTSSP SLPVQNKKV 
        YQKNKKATS GKHRPSSES SEETRIKAQ VTQVLSEYR 
        HPQREYKEY TGSTRYLSD LNEVNHAKT TVHPQVTCN 
        MPKAKQGSN KTSGTNYRK DFAHGVKST RSAGSSYSE 
        DSQSRNNSP RMYHNKYAP MTRF
      CDS sequence
      • >TC001864 RA
        atgctaaag ccggaattc agctgtatt gttgcgttt 
        ttttgggcg ttttggccg cgatcttcg gagaggaag 
        aggttttcg gttgaggaa gctccggag ttgtcgcgg 
        tttcgaatg cggaaatgg tttgaacgc gtggtcaaa 
        ccgaagatc cgttgcagt cgatttgga ctcctcgtg 
        agtggcttg tgtgtggtg agctcggca attttggga 
        attctgccc ttggtgcga ccaggagtt acgcttcgc 
        tgcgacttg aggaaccaa ggcggaaga actgcgctt 
        tgtactgtg gaagacact tttgatggc acttgctac 
        ataatatgt cgggattct tgtggaatt atgggaaac 
        atcggtagc caaactggc caaatcacc caacgaccg 
        ttacctgaa gaacaacaa tgggcacat tcgttccca 
        aggacgcaa ccgcgcctc caccagcat aaagtcctc 
        ccggaacga gacgcccgc caaaaactg cgccatacc 
        aacaatggg gaaatactc cagtccgga gggacgctc 
        agcgggagg caccaccaa aaccgcttg tccttgcga 
        gagaattcg aacgaattt tcaaccttg gataagccc 
        aggacgtat ttgtccaac caacgggac cccttgcac 
        aaatcgggg gcgaggtac acccacgcc atggaatac 
        aaacatttc agtcatagt gcctatagg gaaaatatg 
        aaaaagttc ggctctgag cctgatttg cgccattcg 
        aaggggatg gagactcag gaccggcgg gggaagaag 
        aagtataag gcgcctccc cctcccgtg gaggtgaac 
        aagtcgctc gattgggat gattcaagc ggtgacaat 
        ggccccact cgtcgcgct cgtctcttc aaaacgcgg 
        gccgaaacg cgcaaaaat tactctagt cctgtacat 
        aatcataaa tcattagaa aaacacgag gaattagtc 
        gaaattaga gaccgcgac cgaagtgcg gatttacgt 
        acaaatcga ttgtcgctc cctgaaatg attactcca 
        aacttccat caggaacta aaagaggtg actaaacgc 
        ctaagacac acccgcgcc tcaaccgac aacacgatc 
        gcagaaatc aataaccgg aataaagaa aaattgaaa 
        acactcgat cacaacata cataagaaa gacaacatt 
        aggcaaaga ctgaagcag atggagacc gtggaggag 
        aagctcgac ccggtccga ggggacggc tcaggcaaa 
        gagtcctcc tcccctgac cagtcatcc ccaatcatc 
        cgcaaaaaa gaacaaccc aaaactttc tacttcggc 
        atggacgag accatgcaa aacgaaatc gtcgaccac 
        ttcgcctcc aacctccac tccatgaca cacatcccg 
        aaaattgca aactcatcc gaatcggat atttcgagt 
        gaaatcgaa gccgatgaa aacacaggt gttagaaac 
        ggcattgat ttgcaattg agaccgatc ttgccgaag 
        aaacagtta gaaatcccg aggttctcc ccagctgct 
        gcctggaga ctcctctcc gttgacacc aacccgacc 
        gccagtacc atagcaagt gacgaagtc ccggttttc 
        atcgaagac aggattgag aaatactcg cgaccgcca 
        ccacccacc atgcagatc gggcaaagg tctaacaac 
        gacaaatcc ggcgactcc ggcatttcc ggggacgcc 
        gggcccccg ggatacgac gacagcccc gacgctatt 
        atgaactcc cggaacagt cttctgata ccgaagggg 
        atttcgtgg acgccgcag caagacctc tgcgacgac 
        tccagcatg gaggagggt ctgaataac gaaaactcg 
        tacaagccg cgttccata tactcgaac aaaccgcac 
        gttttcagc ttatcgtta cggggagac aaccacttg 
        tccgcctac atgtcggac aagacatac accggcttg 
        cagaaattg aagcgcgac aattggttc ttgagcaaa 
        tcggccccc aactcgctc aacaacggc tttaactct 
        ctcgaaatg aaaaattcg aacaaatcg gaggaaaaa 
        gaacaatta ttagtgcca aattcgaaa agctcaggt 
        cggttaatg tatctccct gaattcgac aataaccaa 
        gaccaccgt catgcccgc gccaagaca aaagacgat 
        ctccgcgaa cgctccaaa agcgccgat cgctcaaaa 
        accaatcac aaactatcc gtgttttcg aaatcgtgc 
        gaaaacatc aacggcgaa ctcaaatcg tcctcggac 
        gcggaaccc gaagacttg caagatccg ccaaaagtg 
        ttcgaagac gcgaggaac tggaaccgc aaaccgcga 
        agattcacg ttccagagc acagttcgc cagattgag 
        cggcgaaga cttgcggag aaactctca cgggaggct 
        gagaagaag gagaagcaa cgactacgc gagttggag 
        gcgatgcaa aaggtcgag gaagagttc cagaagaag 
        agggcacgg gagaaggcg aacattcgc caacagctg 
        cgccttttc tccatggac gatgtcgcg tggactagt 
        cttcccccc aacttggag gtgaaggac gaagtgcgg 
        caggaaccg gacggtgcg gtgtcctcc tccacgacc 
        tcctccccc tcactccca gtccaaaac aagaaagtt 
        taccaaaaa aataagaag gccacgagc ggcaaacac 
        cgcccgagc tccgaaagt agcgaagag acgcggatt 
        aaagctcaa gtcacgcag gtactgtcg gagtacaga 
        cacccccaa cgggagtac aaggagtat accgggagt 
        acgcggtac ttaagtgac ctgaacgaa gtaaatcat 
        gccaaaacc acggttcat ccacaagtt acgtgcaac 
        atgccaaaa gcgaagcaa ggttcgaat aagactagt 
        gggacgaat tataggaaa gattttgct catggggtg 
        aagagtaca aggtcggca ggaagcagc tattcggag 
        gattcgcag tcgaggaac aattcgccg aggatgtat 
        cataacaaa tatgctcca atgacaagg ttttaa
      mRNA sequence
      • >TC001864 RA
        caccgactt ccacaaaat attctcttc tttcaaaac 
        aaccggccg gcggaaaaa cacttgact catcttcga 
        tgctgaagg aaaattttg aaagcgagc tcgctatca 
        ctttttatt attcattag cagccgcat gaaggaatt 
        tgaggatcg gaagtgaat tagtccacc gcagacatg 
        ctaaagccg gaattcagc tgtattgtt gcgtttttt 
        tgggcgttt tggccgcga tcttcggag aggaagagg 
        ttttcggtt gaggaagct ccggagttg tcgcggttt 
        cgaatgcgg aaatggttt gaacgcgtg gtcaaaccg 
        aagatccgt tgcagtcga tttggactc ctcgtgagt 
        ggcttgtgt gtggtgagc tcggcaatt ttgggaatt 
        ctgcccttg gtgcgacca ggagttacg cttcgctgc 
        gacttgagg aaccaaggc ggaagaact gcgctttgt 
        actgtggaa gacactttt gatggcact tgctacata 
        atatgtcgg gattcttgt ggaattatg ggaaacatc 
        ggtagccaa actggccaa atcacccaa cgaccgtta 
        cctgaagaa caacaatgg gcacattcg ttcccaagg 
        acgcaaccg cgcctccac cagcataaa gtcctcccg 
        gaacgagac gcccgccaa aaactgcgc cataccaac 
        aatggggaa atactccag tccggaggg acgctcagc 
        gggaggcac caccaaaac cgcttgtcc ttgcgagag 
        aattcgaac gaattttca accttggat aagcccagg 
        acgtatttg tccaaccaa cgggacccc ttgcacaaa 
        tcgggggcg aggtacacc cacgccatg gaatacaaa 
        catttcagt catagtgcc tatagggaa aatatgaaa 
        aagttcggc tctgagcct gatttgcgc cattcgaag 
        gggatggag actcaggac cggcggggg aagaagaag 
        tataaggcg cctccccct cccgtggag gtgaacaag 
        tcgctcgat tgggatgat tcaagcggt gacaatggc 
        cccactcgt cgcgctcgt ctcttcaaa acgcgggcc 
        gaaacgcgc aaaaattac tctagtcct gtacataat 
        cataaatca ttagaaaaa cacgaggaa ttagtcgaa 
        attagagac cgcgaccga agtgcggat ttacgtaca 
        aatcgattg tcgctccct gaaatgatt actccaaac 
        ttccatcag gaactaaaa gaggtgact aaacgccta 
        agacacacc cgcgcctca accgacaac acgatcgca 
        gaaatcaat aaccggaat aaagaaaaa ttgaaaaca 
        ctcgatcac aacatacat aagaaagac aacattagg 
        caaagactg aagcagatg gagaccgtg gaggagaag 
        ctcgacccg gtccgaggg gacggctca ggcaaagag 
        tcctcctcc cctgaccag tcatcccca atcatccgc 
        aaaaaagaa caacccaaa actttctac ttcggcatg 
        gacgagacc atgcaaaac gaaatcgtc gaccacttc 
        gcctccaac ctccactcc atgacacac atcccgaaa 
        attgcaaac tcatccgaa tcggatatt tcgagtgaa 
        atcgaagcc gatgaaaac acaggtgtt agaaacggc 
        attgatttg caattgaga ccgatcttg ccgaagaaa 
        cagttagaa atcccgagg ttctcccca gctgctgcc 
        tggagactc ctctccgtt gacaccaac ccgaccgcc 
        agtaccata gcaagtgac gaagtcccg gttttcatc 
        gaagacagg attgagaaa tactcgcga ccgccacca 
        cccaccatg cagatcggg caaaggtct aacaacgac 
        aaatccggc gactccggc atttccggg gacgccggg 
        cccccggga tacgacgac agccccgac gctattatg 
        aactcccgg aacagtctt ctgataccg aaggggatt 
        tcgtggacg ccgcagcaa gacctctgc gacgactcc 
        agcatggag gagggtctg aataacgaa aactcgtac 
        aagccgcgt tccatatac tcgaacaaa ccgcacgtt 
        ttcagctta tcgttacgg ggagacaac cacttgtcc 
        gcctacatg tcggacaag acatacacc ggcttgcag 
        aaattgaag cgcgacaat tggttcttg agcaaatcg 
        gcccccaac tcgctcaac aacggcttt aactctctc 
        gaaatgaaa aattcgaac aaatcggag gaaaaagaa 
        caattatta gtgccaaat tcgaaaagc tcaggtcgg 
        ttaatgtat ctccctgaa ttcgacaat aaccaagac 
        caccgtcat gcccgcgcc aagacaaaa gacgatctc 
        cgcgaacgc tccaaaagc gccgatcgc tcaaaaacc 
        aatcacaaa ctatccgtg ttttcgaaa tcgtgcgaa 
        aacatcaac ggcgaactc aaatcgtcc tcggacgcg 
        gaacccgaa gacttgcaa gatccgcca aaagtgttc 
        gaagacgcg aggaactgg aaccgcaaa ccgcgaaga 
        ttcacgttc cagagcaca gttcgccag attgagcgg 
        cgaagactt gcggagaaa ctctcacgg gaggctgag 
        aagaaggag aagcaacga ctacgcgag ttggaggcg 
        atgcaaaag gtcgaggaa gagttccag aagaagagg 
        gcacgggag aaggcgaac attcgccaa cagctgcgc 
        cttttctcc atggacgat gtcgcgtgg actagtctt 
        ccccccaac ttggaggtg aaggacgaa gtgcggcag 
        gaaccggac ggtgcggtg tcctcctcc acgacctcc 
        tccccctca ctcccagtc caaaacaag aaagtttac 
        caaaaaaat aagaaggcc acgagcggc aaacaccgc 
        ccgagctcc gaaagtagc gaagagacg cggattaaa 
        gctcaagtc acgcaggta ctgtcggag tacagacac 
        ccccaacgg gagtacaag gagtatacc gggagtacg 
        cggtactta agtgacctg aacgaagta aatcatgcc 
        aaaaccacg gttcatcca caagttacg tgcaacatg 
        ccaaaagcg aagcaaggt tcgaataag actagtggg 
        acgaattat aggaaagat tttgctcat ggggtgaag 
        agtacaagg tcggcagga agcagctat tcggaggat 
        tcgcagtcg aggaacaat tcgccgagg atgtatcat 
        aacaaatat gctccaatg acaaggttt taatgtatt 
        attttacag tcaattaat ccatgtacc tatgtattt 
        taacattct catttttac tacaaaatg tatatatac 
        aaatgcaaa ataaatttt aatgattgc t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY