General information | |||
TC001864 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 531953..541688 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC001864 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06709 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax potentially partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
legs podomer sometimes fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001864 RA
MLKPEFSCI VAFFWAFWP RSSERKRFS VEEAPELSR FRMRKWFER VVKPKIRCS RFGLLVSGL CVVSSAILG ILPLVRPGV TLRCDLRNQ GGRTALCTV EDTFDGTCY IICRDSCGI MGNIGSQTG QITQRPLPE EQQWAHSFP RTQPRLHQH KVLPERDAR QKLRHTNNG EILQSGGTL SGRHHQNRL SLRENSNEF STLDKPRTY LSNQRDPLH KSGARYTHA MEYKHFSHS AYRENMKKF GSEPDLRHS KGMETQDRR GKKKYKAPP PPVEVNKSL DWDDSSGDN GPTRRARLF KTRAETRKN YSSPVHNHK SLEKHEELV EIRDRDRSA DLRTNRLSL PEMITPNFH QELKEVTKR LRHTRASTD NTIAEINNR NKEKLKTLD HNIHKKDNI RQRLKQMET VEEKLDPVR GDGSGKESS SPDQSSPII RKKEQPKTF YFGMDETMQ NEIVDHFAS NLHSMTHIP KIANSSESD ISSEIEADE NTGVRNGID LQLRPILPK KQLEIPRFS PAAAWRLLS VDTNPTAST IASDEVPVF IEDRIEKYS RPPPPTMQI GQRSNNDKS GDSGISGDA GPPGYDDSP DAIMNSRNS LLIPKGISW TPQQDLCDD SSMEEGLNN ENSYKPRSI YSNKPHVFS LSLRGDNHL SAYMSDKTY TGLQKLKRD NWFLSKSAP NSLNNGFNS LEMKNSNKS EEKEQLLVP NSKSSGRLM YLPEFDNNQ DHRHARAKT KDDLRERSK SADRSKTNH KLSVFSKSC ENINGELKS SSDAEPEDL QDPPKVFED ARNWNRKPR RFTFQSTVR QIERRRLAE KLSREAEKK EKQRLRELE AMQKVEEEF QKKRAREKA NIRQQLRLF SMDDVAWTS LPPNLEVKD EVRQEPDGA VSSSTTSSP SLPVQNKKV YQKNKKATS GKHRPSSES SEETRIKAQ VTQVLSEYR HPQREYKEY TGSTRYLSD LNEVNHAKT TVHPQVTCN MPKAKQGSN KTSGTNYRK DFAHGVKST RSAGSSYSE DSQSRNNSP RMYHNKYAP MTRF
- >TC001864 RA
atgctaaag ccggaattc agctgtatt gttgcgttt ttttgggcg ttttggccg cgatcttcg gagaggaag aggttttcg gttgaggaa gctccggag ttgtcgcgg tttcgaatg cggaaatgg tttgaacgc gtggtcaaa ccgaagatc cgttgcagt cgatttgga ctcctcgtg agtggcttg tgtgtggtg agctcggca attttggga attctgccc ttggtgcga ccaggagtt acgcttcgc tgcgacttg aggaaccaa ggcggaaga actgcgctt tgtactgtg gaagacact tttgatggc acttgctac ataatatgt cgggattct tgtggaatt atgggaaac atcggtagc caaactggc caaatcacc caacgaccg ttacctgaa gaacaacaa tgggcacat tcgttccca aggacgcaa ccgcgcctc caccagcat aaagtcctc ccggaacga gacgcccgc caaaaactg cgccatacc aacaatggg gaaatactc cagtccgga gggacgctc agcgggagg caccaccaa aaccgcttg tccttgcga gagaattcg aacgaattt tcaaccttg gataagccc aggacgtat ttgtccaac caacgggac cccttgcac aaatcgggg gcgaggtac acccacgcc atggaatac aaacatttc agtcatagt gcctatagg gaaaatatg aaaaagttc ggctctgag cctgatttg cgccattcg aaggggatg gagactcag gaccggcgg gggaagaag aagtataag gcgcctccc cctcccgtg gaggtgaac aagtcgctc gattgggat gattcaagc ggtgacaat ggccccact cgtcgcgct cgtctcttc aaaacgcgg gccgaaacg cgcaaaaat tactctagt cctgtacat aatcataaa tcattagaa aaacacgag gaattagtc gaaattaga gaccgcgac cgaagtgcg gatttacgt acaaatcga ttgtcgctc cctgaaatg attactcca aacttccat caggaacta aaagaggtg actaaacgc ctaagacac acccgcgcc tcaaccgac aacacgatc gcagaaatc aataaccgg aataaagaa aaattgaaa acactcgat cacaacata cataagaaa gacaacatt aggcaaaga ctgaagcag atggagacc gtggaggag aagctcgac ccggtccga ggggacggc tcaggcaaa gagtcctcc tcccctgac cagtcatcc ccaatcatc cgcaaaaaa gaacaaccc aaaactttc tacttcggc atggacgag accatgcaa aacgaaatc gtcgaccac ttcgcctcc aacctccac tccatgaca cacatcccg aaaattgca aactcatcc gaatcggat atttcgagt gaaatcgaa gccgatgaa aacacaggt gttagaaac ggcattgat ttgcaattg agaccgatc ttgccgaag aaacagtta gaaatcccg aggttctcc ccagctgct gcctggaga ctcctctcc gttgacacc aacccgacc gccagtacc atagcaagt gacgaagtc ccggttttc atcgaagac aggattgag aaatactcg cgaccgcca ccacccacc atgcagatc gggcaaagg tctaacaac gacaaatcc ggcgactcc ggcatttcc ggggacgcc gggcccccg ggatacgac gacagcccc gacgctatt atgaactcc cggaacagt cttctgata ccgaagggg atttcgtgg acgccgcag caagacctc tgcgacgac tccagcatg gaggagggt ctgaataac gaaaactcg tacaagccg cgttccata tactcgaac aaaccgcac gttttcagc ttatcgtta cggggagac aaccacttg tccgcctac atgtcggac aagacatac accggcttg cagaaattg aagcgcgac aattggttc ttgagcaaa tcggccccc aactcgctc aacaacggc tttaactct ctcgaaatg aaaaattcg aacaaatcg gaggaaaaa gaacaatta ttagtgcca aattcgaaa agctcaggt cggttaatg tatctccct gaattcgac aataaccaa gaccaccgt catgcccgc gccaagaca aaagacgat ctccgcgaa cgctccaaa agcgccgat cgctcaaaa accaatcac aaactatcc gtgttttcg aaatcgtgc gaaaacatc aacggcgaa ctcaaatcg tcctcggac gcggaaccc gaagacttg caagatccg ccaaaagtg ttcgaagac gcgaggaac tggaaccgc aaaccgcga agattcacg ttccagagc acagttcgc cagattgag cggcgaaga cttgcggag aaactctca cgggaggct gagaagaag gagaagcaa cgactacgc gagttggag gcgatgcaa aaggtcgag gaagagttc cagaagaag agggcacgg gagaaggcg aacattcgc caacagctg cgccttttc tccatggac gatgtcgcg tggactagt cttcccccc aacttggag gtgaaggac gaagtgcgg caggaaccg gacggtgcg gtgtcctcc tccacgacc tcctccccc tcactccca gtccaaaac aagaaagtt taccaaaaa aataagaag gccacgagc ggcaaacac cgcccgagc tccgaaagt agcgaagag acgcggatt aaagctcaa gtcacgcag gtactgtcg gagtacaga cacccccaa cgggagtac aaggagtat accgggagt acgcggtac ttaagtgac ctgaacgaa gtaaatcat gccaaaacc acggttcat ccacaagtt acgtgcaac atgccaaaa gcgaagcaa ggttcgaat aagactagt gggacgaat tataggaaa gattttgct catggggtg aagagtaca aggtcggca ggaagcagc tattcggag gattcgcag tcgaggaac aattcgccg aggatgtat cataacaaa tatgctcca atgacaagg ttttaa
- >TC001864 RA
caccgactt ccacaaaat attctcttc tttcaaaac aaccggccg gcggaaaaa cacttgact catcttcga tgctgaagg aaaattttg aaagcgagc tcgctatca ctttttatt attcattag cagccgcat gaaggaatt tgaggatcg gaagtgaat tagtccacc gcagacatg ctaaagccg gaattcagc tgtattgtt gcgtttttt tgggcgttt tggccgcga tcttcggag aggaagagg ttttcggtt gaggaagct ccggagttg tcgcggttt cgaatgcgg aaatggttt gaacgcgtg gtcaaaccg aagatccgt tgcagtcga tttggactc ctcgtgagt ggcttgtgt gtggtgagc tcggcaatt ttgggaatt ctgcccttg gtgcgacca ggagttacg cttcgctgc gacttgagg aaccaaggc ggaagaact gcgctttgt actgtggaa gacactttt gatggcact tgctacata atatgtcgg gattcttgt ggaattatg ggaaacatc ggtagccaa actggccaa atcacccaa cgaccgtta cctgaagaa caacaatgg gcacattcg ttcccaagg acgcaaccg cgcctccac cagcataaa gtcctcccg gaacgagac gcccgccaa aaactgcgc cataccaac aatggggaa atactccag tccggaggg acgctcagc gggaggcac caccaaaac cgcttgtcc ttgcgagag aattcgaac gaattttca accttggat aagcccagg acgtatttg tccaaccaa cgggacccc ttgcacaaa tcgggggcg aggtacacc cacgccatg gaatacaaa catttcagt catagtgcc tatagggaa aatatgaaa aagttcggc tctgagcct gatttgcgc cattcgaag gggatggag actcaggac cggcggggg aagaagaag tataaggcg cctccccct cccgtggag gtgaacaag tcgctcgat tgggatgat tcaagcggt gacaatggc cccactcgt cgcgctcgt ctcttcaaa acgcgggcc gaaacgcgc aaaaattac tctagtcct gtacataat cataaatca ttagaaaaa cacgaggaa ttagtcgaa attagagac cgcgaccga agtgcggat ttacgtaca aatcgattg tcgctccct gaaatgatt actccaaac ttccatcag gaactaaaa gaggtgact aaacgccta agacacacc cgcgcctca accgacaac acgatcgca gaaatcaat aaccggaat aaagaaaaa ttgaaaaca ctcgatcac aacatacat aagaaagac aacattagg caaagactg aagcagatg gagaccgtg gaggagaag ctcgacccg gtccgaggg gacggctca ggcaaagag tcctcctcc cctgaccag tcatcccca atcatccgc aaaaaagaa caacccaaa actttctac ttcggcatg gacgagacc atgcaaaac gaaatcgtc gaccacttc gcctccaac ctccactcc atgacacac atcccgaaa attgcaaac tcatccgaa tcggatatt tcgagtgaa atcgaagcc gatgaaaac acaggtgtt agaaacggc attgatttg caattgaga ccgatcttg ccgaagaaa cagttagaa atcccgagg ttctcccca gctgctgcc tggagactc ctctccgtt gacaccaac ccgaccgcc agtaccata gcaagtgac gaagtcccg gttttcatc gaagacagg attgagaaa tactcgcga ccgccacca cccaccatg cagatcggg caaaggtct aacaacgac aaatccggc gactccggc atttccggg gacgccggg cccccggga tacgacgac agccccgac gctattatg aactcccgg aacagtctt ctgataccg aaggggatt tcgtggacg ccgcagcaa gacctctgc gacgactcc agcatggag gagggtctg aataacgaa aactcgtac aagccgcgt tccatatac tcgaacaaa ccgcacgtt ttcagctta tcgttacgg ggagacaac cacttgtcc gcctacatg tcggacaag acatacacc ggcttgcag aaattgaag cgcgacaat tggttcttg agcaaatcg gcccccaac tcgctcaac aacggcttt aactctctc gaaatgaaa aattcgaac aaatcggag gaaaaagaa caattatta gtgccaaat tcgaaaagc tcaggtcgg ttaatgtat ctccctgaa ttcgacaat aaccaagac caccgtcat gcccgcgcc aagacaaaa gacgatctc cgcgaacgc tccaaaagc gccgatcgc tcaaaaacc aatcacaaa ctatccgtg ttttcgaaa tcgtgcgaa aacatcaac ggcgaactc aaatcgtcc tcggacgcg gaacccgaa gacttgcaa gatccgcca aaagtgttc gaagacgcg aggaactgg aaccgcaaa ccgcgaaga ttcacgttc cagagcaca gttcgccag attgagcgg cgaagactt gcggagaaa ctctcacgg gaggctgag aagaaggag aagcaacga ctacgcgag ttggaggcg atgcaaaag gtcgaggaa gagttccag aagaagagg gcacgggag aaggcgaac attcgccaa cagctgcgc cttttctcc atggacgat gtcgcgtgg actagtctt ccccccaac ttggaggtg aaggacgaa gtgcggcag gaaccggac ggtgcggtg tcctcctcc acgacctcc tccccctca ctcccagtc caaaacaag aaagtttac caaaaaaat aagaaggcc acgagcggc aaacaccgc ccgagctcc gaaagtagc gaagagacg cggattaaa gctcaagtc acgcaggta ctgtcggag tacagacac ccccaacgg gagtacaag gagtatacc gggagtacg cggtactta agtgacctg aacgaagta aatcatgcc aaaaccacg gttcatcca caagttacg tgcaacatg ccaaaagcg aagcaaggt tcgaataag actagtggg acgaattat aggaaagat tttgctcat ggggtgaag agtacaagg tcggcagga agcagctat tcggaggat tcgcagtcg aggaacaat tcgccgagg atgtatcat aacaaatat gctccaatg acaaggttt taatgtatt attttacag tcaattaat ccatgtacc tatgtattt taacattct catttttac tacaaaatg tatatatac aaatgcaaa ataaatttt aatgattgc t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
