Status
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Gene information
General information
TC001864 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 531953..541688
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC001864
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06709

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      larva segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax potentially partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs podomer sometimes fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001864 RA
        MLKPEFSCI VAFFWAFWP RSSERKRFS VEEAPELSR 
FRMRKWFER VVKPKIRCS RFGLLVSGL CVVSSAILG 
ILPLVRPGV TLRCDLRNQ GGRTALCTV EDTFDGTCY 
IICRDSCGI MGNIGSQTG QITQRPLPE EQQWAHSFP 
RTQPRLHQH KVLPERDAR QKLRHTNNG EILQSGGTL 
SGRHHQNRL SLRENSNEF STLDKPRTY LSNQRDPLH 
KSGARYTHA MEYKHFSHS AYRENMKKF GSEPDLRHS 
KGMETQDRR GKKKYKAPP PPVEVNKSL DWDDSSGDN 
GPTRRARLF KTRAETRKN YSSPVHNHK SLEKHEELV 
EIRDRDRSA DLRTNRLSL PEMITPNFH QELKEVTKR 
LRHTRASTD NTIAEINNR NKEKLKTLD HNIHKKDNI 
RQRLKQMET VEEKLDPVR GDGSGKESS SPDQSSPII 
RKKEQPKTF YFGMDETMQ NEIVDHFAS NLHSMTHIP 
KIANSSESD ISSEIEADE NTGVRNGID LQLRPILPK 
KQLEIPRFS PAAAWRLLS VDTNPTAST IASDEVPVF 
IEDRIEKYS RPPPPTMQI GQRSNNDKS GDSGISGDA 
GPPGYDDSP DAIMNSRNS LLIPKGISW TPQQDLCDD 
SSMEEGLNN ENSYKPRSI YSNKPHVFS LSLRGDNHL 
SAYMSDKTY TGLQKLKRD NWFLSKSAP NSLNNGFNS 
LEMKNSNKS EEKEQLLVP NSKSSGRLM YLPEFDNNQ 
DHRHARAKT KDDLRERSK SADRSKTNH KLSVFSKSC 
ENINGELKS SSDAEPEDL QDPPKVFED ARNWNRKPR 
RFTFQSTVR QIERRRLAE KLSREAEKK EKQRLRELE 
AMQKVEEEF QKKRAREKA NIRQQLRLF SMDDVAWTS 
LPPNLEVKD EVRQEPDGA VSSSTTSSP SLPVQNKKV 
YQKNKKATS GKHRPSSES SEETRIKAQ VTQVLSEYR 
HPQREYKEY TGSTRYLSD LNEVNHAKT TVHPQVTCN 
MPKAKQGSN KTSGTNYRK DFAHGVKST RSAGSSYSE 
DSQSRNNSP RMYHNKYAP MTRF
      CDS sequence
      • >TC001864 RA
        atgctaaag ccggaattc agctgtatt gttgcgttt 
ttttgggcg ttttggccg cgatcttcg gagaggaag 
aggttttcg gttgaggaa gctccggag ttgtcgcgg 
tttcgaatg cggaaatgg tttgaacgc gtggtcaaa 
ccgaagatc cgttgcagt cgatttgga ctcctcgtg 
agtggcttg tgtgtggtg agctcggca attttggga 
attctgccc ttggtgcga ccaggagtt acgcttcgc 
tgcgacttg aggaaccaa ggcggaaga actgcgctt 
tgtactgtg gaagacact tttgatggc acttgctac 
ataatatgt cgggattct tgtggaatt atgggaaac 
atcggtagc caaactggc caaatcacc caacgaccg 
ttacctgaa gaacaacaa tgggcacat tcgttccca 
aggacgcaa ccgcgcctc caccagcat aaagtcctc 
ccggaacga gacgcccgc caaaaactg cgccatacc 
aacaatggg gaaatactc cagtccgga gggacgctc 
agcgggagg caccaccaa aaccgcttg tccttgcga 
gagaattcg aacgaattt tcaaccttg gataagccc 
aggacgtat ttgtccaac caacgggac cccttgcac 
aaatcgggg gcgaggtac acccacgcc atggaatac 
aaacatttc agtcatagt gcctatagg gaaaatatg 
aaaaagttc ggctctgag cctgatttg cgccattcg 
aaggggatg gagactcag gaccggcgg gggaagaag 
aagtataag gcgcctccc cctcccgtg gaggtgaac 
aagtcgctc gattgggat gattcaagc ggtgacaat 
ggccccact cgtcgcgct cgtctcttc aaaacgcgg 
gccgaaacg cgcaaaaat tactctagt cctgtacat 
aatcataaa tcattagaa aaacacgag gaattagtc 
gaaattaga gaccgcgac cgaagtgcg gatttacgt 
acaaatcga ttgtcgctc cctgaaatg attactcca 
aacttccat caggaacta aaagaggtg actaaacgc 
ctaagacac acccgcgcc tcaaccgac aacacgatc 
gcagaaatc aataaccgg aataaagaa aaattgaaa 
acactcgat cacaacata cataagaaa gacaacatt 
aggcaaaga ctgaagcag atggagacc gtggaggag 
aagctcgac ccggtccga ggggacggc tcaggcaaa 
gagtcctcc tcccctgac cagtcatcc ccaatcatc 
cgcaaaaaa gaacaaccc aaaactttc tacttcggc 
atggacgag accatgcaa aacgaaatc gtcgaccac 
ttcgcctcc aacctccac tccatgaca cacatcccg 
aaaattgca aactcatcc gaatcggat atttcgagt 
gaaatcgaa gccgatgaa aacacaggt gttagaaac 
ggcattgat ttgcaattg agaccgatc ttgccgaag 
aaacagtta gaaatcccg aggttctcc ccagctgct 
gcctggaga ctcctctcc gttgacacc aacccgacc 
gccagtacc atagcaagt gacgaagtc ccggttttc 
atcgaagac aggattgag aaatactcg cgaccgcca 
ccacccacc atgcagatc gggcaaagg tctaacaac 
gacaaatcc ggcgactcc ggcatttcc ggggacgcc 
gggcccccg ggatacgac gacagcccc gacgctatt 
atgaactcc cggaacagt cttctgata ccgaagggg 
atttcgtgg acgccgcag caagacctc tgcgacgac 
tccagcatg gaggagggt ctgaataac gaaaactcg 
tacaagccg cgttccata tactcgaac aaaccgcac 
gttttcagc ttatcgtta cggggagac aaccacttg 
tccgcctac atgtcggac aagacatac accggcttg 
cagaaattg aagcgcgac aattggttc ttgagcaaa 
tcggccccc aactcgctc aacaacggc tttaactct 
ctcgaaatg aaaaattcg aacaaatcg gaggaaaaa 
gaacaatta ttagtgcca aattcgaaa agctcaggt 
cggttaatg tatctccct gaattcgac aataaccaa 
gaccaccgt catgcccgc gccaagaca aaagacgat 
ctccgcgaa cgctccaaa agcgccgat cgctcaaaa 
accaatcac aaactatcc gtgttttcg aaatcgtgc 
gaaaacatc aacggcgaa ctcaaatcg tcctcggac 
gcggaaccc gaagacttg caagatccg ccaaaagtg 
ttcgaagac gcgaggaac tggaaccgc aaaccgcga 
agattcacg ttccagagc acagttcgc cagattgag 
cggcgaaga cttgcggag aaactctca cgggaggct 
gagaagaag gagaagcaa cgactacgc gagttggag 
gcgatgcaa aaggtcgag gaagagttc cagaagaag 
agggcacgg gagaaggcg aacattcgc caacagctg 
cgccttttc tccatggac gatgtcgcg tggactagt 
cttcccccc aacttggag gtgaaggac gaagtgcgg 
caggaaccg gacggtgcg gtgtcctcc tccacgacc 
tcctccccc tcactccca gtccaaaac aagaaagtt 
taccaaaaa aataagaag gccacgagc ggcaaacac 
cgcccgagc tccgaaagt agcgaagag acgcggatt 
aaagctcaa gtcacgcag gtactgtcg gagtacaga 
cacccccaa cgggagtac aaggagtat accgggagt 
acgcggtac ttaagtgac ctgaacgaa gtaaatcat 
gccaaaacc acggttcat ccacaagtt acgtgcaac 
atgccaaaa gcgaagcaa ggttcgaat aagactagt 
gggacgaat tataggaaa gattttgct catggggtg 
aagagtaca aggtcggca ggaagcagc tattcggag 
gattcgcag tcgaggaac aattcgccg aggatgtat 
cataacaaa tatgctcca atgacaagg ttttaa
      mRNA sequence
      • >TC001864 RA
        caccgactt ccacaaaat attctcttc tttcaaaac 
aaccggccg gcggaaaaa cacttgact catcttcga 
tgctgaagg aaaattttg aaagcgagc tcgctatca 
ctttttatt attcattag cagccgcat gaaggaatt 
tgaggatcg gaagtgaat tagtccacc gcagacatg 
ctaaagccg gaattcagc tgtattgtt gcgtttttt 
tgggcgttt tggccgcga tcttcggag aggaagagg 
ttttcggtt gaggaagct ccggagttg tcgcggttt 
cgaatgcgg aaatggttt gaacgcgtg gtcaaaccg 
aagatccgt tgcagtcga tttggactc ctcgtgagt 
ggcttgtgt gtggtgagc tcggcaatt ttgggaatt 
ctgcccttg gtgcgacca ggagttacg cttcgctgc 
gacttgagg aaccaaggc ggaagaact gcgctttgt 
actgtggaa gacactttt gatggcact tgctacata 
atatgtcgg gattcttgt ggaattatg ggaaacatc 
ggtagccaa actggccaa atcacccaa cgaccgtta 
cctgaagaa caacaatgg gcacattcg ttcccaagg 
acgcaaccg cgcctccac cagcataaa gtcctcccg 
gaacgagac gcccgccaa aaactgcgc cataccaac 
aatggggaa atactccag tccggaggg acgctcagc 
gggaggcac caccaaaac cgcttgtcc ttgcgagag 
aattcgaac gaattttca accttggat aagcccagg 
acgtatttg tccaaccaa cgggacccc ttgcacaaa 
tcgggggcg aggtacacc cacgccatg gaatacaaa 
catttcagt catagtgcc tatagggaa aatatgaaa 
aagttcggc tctgagcct gatttgcgc cattcgaag 
gggatggag actcaggac cggcggggg aagaagaag 
tataaggcg cctccccct cccgtggag gtgaacaag 
tcgctcgat tgggatgat tcaagcggt gacaatggc 
cccactcgt cgcgctcgt ctcttcaaa acgcgggcc 
gaaacgcgc aaaaattac tctagtcct gtacataat 
cataaatca ttagaaaaa cacgaggaa ttagtcgaa 
attagagac cgcgaccga agtgcggat ttacgtaca 
aatcgattg tcgctccct gaaatgatt actccaaac 
ttccatcag gaactaaaa gaggtgact aaacgccta 
agacacacc cgcgcctca accgacaac acgatcgca 
gaaatcaat aaccggaat aaagaaaaa ttgaaaaca 
ctcgatcac aacatacat aagaaagac aacattagg 
caaagactg aagcagatg gagaccgtg gaggagaag 
ctcgacccg gtccgaggg gacggctca ggcaaagag 
tcctcctcc cctgaccag tcatcccca atcatccgc 
aaaaaagaa caacccaaa actttctac ttcggcatg 
gacgagacc atgcaaaac gaaatcgtc gaccacttc 
gcctccaac ctccactcc atgacacac atcccgaaa 
attgcaaac tcatccgaa tcggatatt tcgagtgaa 
atcgaagcc gatgaaaac acaggtgtt agaaacggc 
attgatttg caattgaga ccgatcttg ccgaagaaa 
cagttagaa atcccgagg ttctcccca gctgctgcc 
tggagactc ctctccgtt gacaccaac ccgaccgcc 
agtaccata gcaagtgac gaagtcccg gttttcatc 
gaagacagg attgagaaa tactcgcga ccgccacca 
cccaccatg cagatcggg caaaggtct aacaacgac 
aaatccggc gactccggc atttccggg gacgccggg 
cccccggga tacgacgac agccccgac gctattatg 
aactcccgg aacagtctt ctgataccg aaggggatt 
tcgtggacg ccgcagcaa gacctctgc gacgactcc 
agcatggag gagggtctg aataacgaa aactcgtac 
aagccgcgt tccatatac tcgaacaaa ccgcacgtt 
ttcagctta tcgttacgg ggagacaac cacttgtcc 
gcctacatg tcggacaag acatacacc ggcttgcag 
aaattgaag cgcgacaat tggttcttg agcaaatcg 
gcccccaac tcgctcaac aacggcttt aactctctc 
gaaatgaaa aattcgaac aaatcggag gaaaaagaa 
caattatta gtgccaaat tcgaaaagc tcaggtcgg 
ttaatgtat ctccctgaa ttcgacaat aaccaagac 
caccgtcat gcccgcgcc aagacaaaa gacgatctc 
cgcgaacgc tccaaaagc gccgatcgc tcaaaaacc 
aatcacaaa ctatccgtg ttttcgaaa tcgtgcgaa 
aacatcaac ggcgaactc aaatcgtcc tcggacgcg 
gaacccgaa gacttgcaa gatccgcca aaagtgttc 
gaagacgcg aggaactgg aaccgcaaa ccgcgaaga 
ttcacgttc cagagcaca gttcgccag attgagcgg 
cgaagactt gcggagaaa ctctcacgg gaggctgag 
aagaaggag aagcaacga ctacgcgag ttggaggcg 
atgcaaaag gtcgaggaa gagttccag aagaagagg 
gcacgggag aaggcgaac attcgccaa cagctgcgc 
cttttctcc atggacgat gtcgcgtgg actagtctt 
ccccccaac ttggaggtg aaggacgaa gtgcggcag 
gaaccggac ggtgcggtg tcctcctcc acgacctcc 
tccccctca ctcccagtc caaaacaag aaagtttac 
caaaaaaat aagaaggcc acgagcggc aaacaccgc 
ccgagctcc gaaagtagc gaagagacg cggattaaa 
gctcaagtc acgcaggta ctgtcggag tacagacac 
ccccaacgg gagtacaag gagtatacc gggagtacg 
cggtactta agtgacctg aacgaagta aatcatgcc 
aaaaccacg gttcatcca caagttacg tgcaacatg 
ccaaaagcg aagcaaggt tcgaataag actagtggg 
acgaattat aggaaagat tttgctcat ggggtgaag 
agtacaagg tcggcagga agcagctat tcggaggat 
tcgcagtcg aggaacaat tcgccgagg atgtatcat 
aacaaatat gctccaatg acaaggttt taatgtatt 
attttacag tcaattaat ccatgtacc tatgtattt 
taacattct catttttac tacaaaatg tatatatac 
aaatgcaaa ataaatttt aatgattgc t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY