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Gene information
General information
TC004264 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28384307..28390381
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004264
    iBeetle screen iB_06708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004264 RA
      MLRARSEQY ERNGVTDHN QGYERKDIK CCVCQKQFS 
      AVFNLAKHA RKYHSVDII PKKSYKYQC LQCNKHFSL 
      LKNFNYHKK THNQSHNPP SKNTYDAVC PICQLKAPK 
      DNLILHFKV EHDIKCETL AIEFTTFEE FSKWKSDME 
      HDTKSKYVA ERGSQGAKD GTTIIKYCC HRSGKYVAR 
      GKGLRHLKT QGSNKIDGF CPARLKVII DKNNQCKVS 
      FIKQHVGHD EEDLGHLFL TLQERKCLA NKMAEDERN 
      LLISEDPKA SEVSGIVTG LTVNEALHP KEFDKQFIA 
      KERQTCMEQ LEAAKNMCI ALEPTLQAI ATKRELTTP 
      STRASPSNK KLTPQRGVL HSTKKKNKR PSMTLVAPT 
      NEEMQAIAI RTLSK
    CDS sequence
    • >TC004264 RA
      atgttgcga gcgaggtcg gaacagtac gaacggaac 
      ggagtaacg gaccacaac cagggctat gaaaggaaa 
      gatatcaaa tgttgtgtg tgccaaaaa cagtttagt 
      gcagttttt aatttggca aaacacgcg cggaagtat 
      cattcagtt gatatcatc ccaaagaag agttacaaa 
      taccagtgt ttacaatgt aacaaacat ttttccctg 
      ttaaaaaat tttaattac cataagaag acgcataac 
      caatcgcat aacccacct tcaaagaac acatatgat 
      gctgtgtgt ccgatttgc cagttgaag gcaccaaaa 
      gataatttg atattgcat tttaaagta gagcatgat 
      ataaaatgt gaaacacta gcaatagaa tttactacc 
      tttgaggaa ttctcaaaa tggaaatca gatatggaa 
      catgatacc aaaagtaaa tatgttgca gagcgggga 
      tctcaaggt gctaaagat gggaccaca ataatcaag 
      tactgttgt catcgttct gggaaatat gttgctcgc 
      gggaaaggg ttaagacat ttaaaaaca caaggttca 
      aataaaatt gacggtttc tgccctgct cgtttaaaa 
      gtaatcatc gacaagaac aaccagtgt aaagtgtct 
      tttatcaaa caacacgtg ggtcacgat gaagaagat 
      ttggggcat ctattcctc acccttcag gagagaaaa 
      tgtttggca aacaaaatg gcagaagat gaacgtaat 
      ttgctgatc agcgaagat cccaaagcc tctgaagtt 
      tccggaatt gtaaccggc cttaccgtc aatgaagct 
      ctacacccc aaagagttt gacaagcag tttattgcc 
      aaagaaagg cagacttgc atggaacaa cttgaagct 
      gcgaaaaac atgtgtata gctctggaa cccacactg 
      caagcaata gcaacgaaa agggagcta acaacaccg 
      tctacacgt gcaagtcct agcaacaaa aaacttaca 
      cctcaacga ggtgtattg cattccacc aagaaaaaa 
      aacaagaga ccatcaatg actctagtt gcgcctaca 
      aatgaagag atgcaagca atcgcgata cgaacgttg 
      tccaaataa
    mRNA sequence
    • >TC004264 RA
      cagttcaat tgttgaaac taatgtaac tcgcaaacc 
      ttcaatagt actttttgt taaagtccg attttttgt 
      gcacatccg acgcatgtg ccctgtagc cgaccatac 
      aagtgataa ctagcggct aggccccgc cgctctctg 
      tcgaaatgt tgcgagcga ggtcggaac agtacgaac 
      ggaacggag taacggacc acaaccagg gctatgaaa 
      ggaaagata tcaaatgtt gtgtgtgcc aaaaacagt 
      ttagtgcag tttttaatt tggcaaaac acgcgcgga 
      agtatcatt cagttgata tcatcccaa agaagagtt 
      acaaatacc agtgtttac aatgtaaca aacattttt 
      ccctgttaa aaaatttta attaccata agaagacgc 
      ataaccaat cgcataacc caccttcaa agaacacat 
      atgatgctg tgtgtccga tttgccagt tgaaggcac 
      caaaagata atttgatat tgcatttta aagtagagc 
      atgatataa aatgtgaaa cactagcaa tagaattta 
      ctacctttg aggaattct caaaatgga aatcagata 
      tggaacatg ataccaaaa gtaaatatg ttgcagagc 
      ggggatctc aaggtgcta aagatggga ccacaataa 
      tcaagtact gttgtcatc gttctggga aatatgttg 
      ctcgcggga aagggttaa gacatttaa aaacacaag 
      gttcaaata aaattgacg gtttctgcc ctgctcgtt 
      taaaagtaa tcatcgaca agaacaacc agtgtaaag 
      tgtctttta tcaaacaac acgtgggtc acgatgaag 
      aagatttgg ggcatctat tcctcaccc ttcaggaga 
      gaaaatgtt tggcaaaca aaatggcag aagatgaac 
      gtaatttgc tgatcagcg aagatccca aagcctctg 
      aagtttccg gaattgtaa ccggcctta ccgtcaatg 
      aagctctac accccaaag agtttgaca agcagttta 
      ttgccaaag aaaggcaga cttgcatgg aacaacttg 
      aagctgcga aaaacatgt gtatagctc tggaaccca 
      cactgcaag caatagcaa cgaaaaggg agctaacaa 
      caccgtcta cacgtgcaa gtcctagca acaaaaaac 
      ttacacctc aacgaggtg tattgcatt ccaccaaga 
      aaaaaaaca agagaccat caatgactc tagttgcgc 
      ctacaaatg aagagatgc aagcaatcg cgatacgaa 
      cgttgtcca aataatttt actactgaa caataatta 
      actttaata aatacttac gcattag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY