Status
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Gene information
General information
TC002371 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 209596..215597
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002371
    iBeetle screen iB_06706

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002371 RA
      MVVSKGGNG RWLRKYKTF FLIGLIILL FQVLLAAHF 
      LELKANSSD DWQPQSHKA VTQGLENHR KKLQVANTT 
      ALRLEELNF RPECEITGK EAVSAIHRA KSQNCKQLI 
      ANVTCKSLA GTLYPKRLT GSCPNGGKI AGKALGCFK 
      DENNYRLLS GYFGVNKKS NSPEYCIQL CLQSGFEYA 
      GVQYSHECF CGNDEPLST FKLPDSSCN MKCPGDPHA 
      TCGGYYTIN VFQTGIKKF VPQVANTES PPSHENVKI 
      VFLLTLNGR ALRQVKRLL KILYHTRHF YYIHVDVRE 
      DYLFRELLP LERRFPNIR LTRRRFATI WGGASLLEM 
      LLSCMSELL DTPWTWDFV LNLSESDYP VKQISALER 
      FLGANRDRN FVKSHGRDT QRFLQKQGL DKTFVECDR 
      RMWRVADRR LPEGIQMDG GSDWIALSR EFVSYVAKS 
      GDDLVGGLR QVFRHTLLP AESFFHTVL RNSRFCDSY 
      VDNNLHVTN WKRKLGCKC QYKHVVDWC GCSPNDFRP 
      DDWARIQST QPRQLFFAR KFEPIINQA VLLKLELWL 
      FGLDKPSRS VTNLHSYWQ SVYHHLDLS PAIDDGLRT 
      VTSSVGRVW SKTVTNATC SVTILRATS FHTKDLYKY 
      TLVHFTTSD SADIEVAFR PINMTYLVA PSTLTKRIE 
      ELAVSSDYD QKEQISRNF PRILSPYSE PVLVYKFAA 
      SGASVTHNI TCLWLDPIG QLVEISDIS IEESALIGH 
      SKPNLKHPH RPGSWTVKL IYHQTLLAE VKFLITPLE 
      FYSGVPLGV KQARLIHGG SVSRPLTDN YDRFIENSD 
      EGILKGVSV SNSNKTGKE LQDWIDSLF SKFYVIEKS 
      CAVGRTEVC GVQVEECRR TSWSSFAPD PKSFIGSVN 
      ETSGAFDIW
    CDS sequence
    • >TC002371 RA
      atggttgtg tccaagggc ggtaacgga agatggctc 
      agaaagtac aaaacgttt tttctcatc ggtttaata 
      atcctgctc ttccaagtg ctcctggct gcgcatttt 
      ctcgagtta aaggcaaac tcatcagac gattggcag 
      ccacaaagc cacaaagcg gtgacacaa ggactagaa 
      aatcaccga aaaaaatta caagtcgca aacaccaca 
      gctttgcgc ttggaagag ctaaacttc agacctgaa 
      tgtgaaatc actggaaag gaagcagtt tcggcaata 
      catcgtgcc aagagccag aattgtaaa caactgatc 
      gcaaatgtg acttgtaag tctttggcg gggactttg 
      taccccaaa cgactgacc ggaagttgt cccaatggc 
      gggaaaatc gccggaaag gccttgggt tgcttcaag 
      gatgaaaat aattacaga ctgctcagt ggctacttt 
      ggggtcaat aaaaagagc aattcgccg gagtattgc 
      atacagttg tgtctccaa tccgggttt gagtacgcc 
      ggggtccaa tattcacat gagtgtttt tgtggcaat 
      gatgagccc ctttcaacg ttcaaacta cccgattcg 
      tcgtgtaat atgaagtgt cccggggac ccccatgct 
      acctgtgga ggctattac accattaac gtgtttcag 
      acgggaata aaaaagttc gtccctcaa gttgccaac 
      actgagtcg cccccaagc cacgaaaac gtcaaaatc 
      gtattcctg ctcaccctc aacggccga gccctccgc 
      caagtgaaa agactgctc aaaatcctc tatcacact 
      cgccacttc tactacatc cacgttgac gtgcgagaa 
      gactacctt ttccgcgaa ctcctcccc ctggagcgc 
      cgcttcccc aacatccgc ctcactcgc cggcgcttc 
      gccaccatc tggggcggc gcctccctc ctggagatg 
      ctcctgagc tgcatgtcc gagctcctg gacacgccc 
      tggacctgg gacttcgtc ctcaacctc agcgagtcc 
      gactacccc gtcaagcaa atctccgcg ctggagcgc 
      ttcctgggg gcgaaccgc gaccgcaac ttcgtcaag 
      tcccacggc agggacacc cagcgcttc ttgcagaag 
      caaggcctg gacaagacc ttcgtcgaa tgcgaccgg 
      cgcatgtgg cgcgtggcg gaccgccgt ctcccggaa 
      ggcatccaa atggacggt gggagcgac tggatcgcc 
      ctctcgcgg gagtttgtg agttacgtg gcgaaaagc 
      ggtgatgat ctagtcggc gggcttagg caggttttt 
      agacatacg ttattgccg gccgagtcg ttttttcat 
      acagttttg cgtaattcg cgtttttgc gacagttat 
      gtcgataat aatcttcac gtgaccaat tggaagcgg 
      aagctgggt tgtaaatgt cagtataag cacgttgtt 
      gattggtgc gggtgtagt ccgaacgat ttcaggccc 
      gatgattgg gccaggatc caaagtacg caaccgcgt 
      cagttattt ttcgcgcgc aagtttgaa ccaatcatc 
      aatcaggcc gttctgttg aaactcgaa ctgtggctg 
      ttcggattg gataaaccg tcaaggtca gtcacgaac 
      ttgcattcc tattggcag agcgtctac caccatttg 
      gacctatca ccggccatt gatgacggt ttaagaact 
      gttacttcg tcagttggc cgtgtgtgg tccaaaacc 
      gtaactaac gccacctgt agtgttaca attttacgt 
      gcaacatca ttccacacc aaagacttg tacaaatat 
      actttggtc cactttaca acaagtgat tcggccgat 
      attgaagtt gctttccga ccgatcaac atgacatat 
      ctcgtagcg ccctctaca ctcaccaaa cgtatcgaa 
      gaactagcc gtcagcagt gactacgac cagaaagaa 
      caaatttcg cggaacttc ccccgaata ttatcaccc 
      tactccgaa ccagtccta gtttacaaa ttcgccgcg 
      agtggcgcc agtgtcacc cacaacatc acgtgtctt 
      tggctcgac cccattggc cagctcgtc gaaatcagc 
      gacatatca atcgaggaa agcgctctg attggccac 
      tccaagccc aatctgaaa cacccccac cggcccggc 
      tcatggacc gttaaacta atctaccac caaactctt 
      ctagctgaa gttaaattc ctaataacc ccgcttgaa 
      ttttactcg ggggttcct ctgggtgtg aaacaagcc 
      cgcttgatt catggcggt tctgtttca aggccactt 
      accgataac tacgatagg tttatcgaa aatagtgac 
      gagggtatt ttgaaaggt gtgagtgtg tctaattcc 
      aacaaaaca ggaaaggaa ttgcaggat tggatcgat 
      tcgctgttt agtaagttt tacgtgatt gagaaaagt 
      tgtgcggtt ggtaggact gaggtttgt ggggtgcaa 
      gtcgaggag tgcaggaga acgagttgg agttctttt 
      gcgcccgat ccgaagagt tttattggg agtgttaat 
      gagacgagt ggcgcgttt gatatctgg taa
    mRNA sequence
    • >TC002371 RA
      aagcactta aaagacact caagtatac gtgtaatgt 
      ggtttcctc ataaaaccg ccaactttt tgttgaggt 
      tatgtcgta aaccagtcc ttagctgac gcccccgtt 
      gcacccaca catgtgtga ttaacgact gtttaaggg 
      gttgcaacc ctgttgaaa catcttcgt ctcttttat 
      acaacttta ggaaaaatt agcctgatt ataatgtga 
      taccgtctc cacgtctaa ccaccaaat cttattaaa 
      aaatccaaa atgtgaggt tatatttgt aagtgatgg 
      gggctgcat tgttctgag atggttgtg tccaagggc 
      ggtaacgga agatggctc agaaagtac aaaacgttt 
      tttctcatc ggtttaata atcctgctc ttccaagtg 
      ctcctggct gcgcatttt ctcgagtta aaggcaaac 
      tcatcagac gattggcag ccacaaagc cacaaagcg 
      gtgacacaa ggactagaa aatcaccga aaaaaatta 
      caagtcgca aacaccaca gctttgcgc ttggaagag 
      ctaaacttc agacctgaa tgtgaaatc actggaaag 
      gaagcagtt tcggcaata catcgtgcc aagagccag 
      aattgtaaa caactgatc gcaaatgtg acttgtaag 
      tctttggcg gggactttg taccccaaa cgactgacc 
      ggaagttgt cccaatggc gggaaaatc gccggaaag 
      gccttgggt tgcttcaag gatgaaaat aattacaga 
      ctgctcagt ggctacttt ggggtcaat aaaaagagc 
      aattcgccg gagtattgc atacagttg tgtctccaa 
      tccgggttt gagtacgcc ggggtccaa tattcacat 
      gagtgtttt tgtggcaat gatgagccc ctttcaacg 
      ttcaaacta cccgattcg tcgtgtaat atgaagtgt 
      cccggggac ccccatgct acctgtgga ggctattac 
      accattaac gtgtttcag acgggaata aaaaagttc 
      gtccctcaa gttgccaac actgagtcg cccccaagc 
      cacgaaaac gtcaaaatc gtattcctg ctcaccctc 
      aacggccga gccctccgc caagtgaaa agactgctc 
      aaaatcctc tatcacact cgccacttc tactacatc 
      cacgttgac gtgcgagaa gactacctt ttccgcgaa 
      ctcctcccc ctggagcgc cgcttcccc aacatccgc 
      ctcactcgc cggcgcttc gccaccatc tggggcggc 
      gcctccctc ctggagatg ctcctgagc tgcatgtcc 
      gagctcctg gacacgccc tggacctgg gacttcgtc 
      ctcaacctc agcgagtcc gactacccc gtcaagcaa 
      atctccgcg ctggagcgc ttcctgggg gcgaaccgc 
      gaccgcaac ttcgtcaag tcccacggc agggacacc 
      cagcgcttc ttgcagaag caaggcctg gacaagacc 
      ttcgtcgaa tgcgaccgg cgcatgtgg cgcgtggcg 
      gaccgccgt ctcccggaa ggcatccaa atggacggt 
      gggagcgac tggatcgcc ctctcgcgg gagtttgtg 
      agttacgtg gcgaaaagc ggtgatgat ctagtcggc 
      gggcttagg caggttttt agacatacg ttattgccg 
      gccgagtcg ttttttcat acagttttg cgtaattcg 
      cgtttttgc gacagttat gtcgataat aatcttcac 
      gtgaccaat tggaagcgg aagctgggt tgtaaatgt 
      cagtataag cacgttgtt gattggtgc gggtgtagt 
      ccgaacgat ttcaggccc gatgattgg gccaggatc 
      caaagtacg caaccgcgt cagttattt ttcgcgcgc 
      aagtttgaa ccaatcatc aatcaggcc gttctgttg 
      aaactcgaa ctgtggctg ttcggattg gataaaccg 
      tcaaggtca gtcacgaac ttgcattcc tattggcag 
      agcgtctac caccatttg gacctatca ccggccatt 
      gatgacggt ttaagaact gttacttcg tcagttggc 
      cgtgtgtgg tccaaaacc gtaactaac gccacctgt 
      agtgttaca attttacgt gcaacatca ttccacacc 
      aaagacttg tacaaatat actttggtc cactttaca 
      acaagtgat tcggccgat attgaagtt gctttccga 
      ccgatcaac atgacatat ctcgtagcg ccctctaca 
      ctcaccaaa cgtatcgaa gaactagcc gtcagcagt 
      gactacgac cagaaagaa caaatttcg cggaacttc 
      ccccgaata ttatcaccc tactccgaa ccagtccta 
      gtttacaaa ttcgccgcg agtggcgcc agtgtcacc 
      cacaacatc acgtgtctt tggctcgac cccattggc 
      cagctcgtc gaaatcagc gacatatca atcgaggaa 
      agcgctctg attggccac tccaagccc aatctgaaa 
      cacccccac cggcccggc tcatggacc gttaaacta 
      atctaccac caaactctt ctagctgaa gttaaattc 
      ctaataacc ccgcttgaa ttttactcg ggggttcct 
      ctgggtgtg aaacaagcc cgcttgatt catggcggt 
      tctgtttca aggccactt accgataac tacgatagg 
      tttatcgaa aatagtgac gagggtatt ttgaaaggt 
      gtgagtgtg tctaattcc aacaaaaca ggaaaggaa 
      ttgcaggat tggatcgat tcgctgttt agtaagttt 
      tacgtgatt gagaaaagt tgtgcggtt ggtaggact 
      gaggtttgt ggggtgcaa gtcgaggag tgcaggaga 
      acgagttgg agttctttt gcgcccgat ccgaagagt 
      tttattggg agtgttaat gagacgagt ggcgcgttt 
      gatatctgg taattgtga tacacattc ggtttgatt 
      gttagcgca ataattaac atgaaatgg ggaaaataa 
      aataaaaac agggtgatg caattttag ttcactgtc 
      tggtatctg tttcagaaa ttgagtgtt ggcgagttc 
      agtcaagaa aaatttttg cgtcacatg tcgatatct 
      cgtgttttt tttaattaa ctgatgata atataacgt 
      tcttaggaa aaaattcaa atccgagct gtttgttcg 
      gtctgtttt ggcgaaaac gcataagaa actaagatc 
      ttagttcaa aagtgtgct tatatgccc aaagaatgt 
      aaaaataac atccttctt ttcgttctc atgtttatt 
      tttgacgaa aattttttt ttgaaaccg ttaatatta 
      catgaattt tattttttt tttagattt taagaattt 
      ttttacatg atagatcta atagttttt taaaattaa 
      ctaaagatg ttaatgaga acgatgttg ccagctcac 
      agtagttaa attctccaa agccaactt gaaaaattc 
      cgagatatg cacttattt tcgccagat tacaatatt 
      tgatataaa tttttggga gagtgtaaa aaatttagg 
      gctggttta cgcaacatt taaatcgca tttcaattt 
      aagttcgcc tttaacttg acgttcgtc aatgcaaaa 
      taaactgca atttaatgc gaactagtt taattcaga 
      attaaattt taattgcgc tttctgtaa accgtctct 
      atgtctcat ttgcatttt ttagagcga aatagaggg 
      tttctgtat aaaaataga attctgtgt ttaactaaa 
      tcccatcta ctttagagc caaggaaat ccatcaatt 
      taaggagaa aaaatactt tgaaaaaag accgagttt 
      ttagctcat aactgctca aaatgtgtt taaatggtt 
      aaaaaagac aattttgaa cataaccgt gatttttcg 
      gatttaatt tatcgaaat tgtgtcata tatacacca 
      taaaatatc tactatggt gattttttt caatagata 
      tctgtttaa gattgcaag gtccgccat tttcgttaa 
      caaaattca agaaagtac aataaaaaa agcaaaaaa 
      aa
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