Status
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Gene information
General information
TC002341 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 192858..194124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002341
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06705

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002341 RA
      MRRPIFVLF FLLHLKNAR VILPTVPDV MHPPVPDDA 
      KVDRDMAFM MEDPDPEVT PGLFQGDMA MTNEIYNYW 
      RVGIRWDVF PEKLWVNGT VPYVISPIY EPSDQVTIL 
      KAIHILNFM TCVRFVPWD GKAKDYLLI WPIKYPKGC 
      WSFVGRFGG AQIVSLQPP DATSPNCLG TEGRAVHEL 
      MHALGVFHE QSRWDRDKF VKIHMDNII PQYKTNFDK 
      QSLENTTYS FEYDYDSIM HYGKYFFSK GKNKPTITA 
      KQPGIKRLG QRKALSKGD CLKLNDLYG CFDNPRLRR 
      KYYTLCQFM GI
    CDS sequence
    • >TC002341 RA
      atgcggcgg ccaattttc gtgttattt tttctgcta 
      catctgaaa aatgccaga gttatcctt ccgacggta 
      cccgatgtc atgcatcca cccgtaccc gacgatgct 
      aaagtcgac cgagatatg gcttttatg atggaagac 
      cccgatccc gaagtcact cccggactg ttccaaggg 
      gacatggct atgaccaac gagatctac aactactgg 
      agggtaggg atacggtgg gacgtgttt ccggaaaaa 
      ctatgggtc aatgggact gttccatat gtcataagc 
      cccatatat gaaccatcg gaccaagtg acaatttta 
      aaagccatc catattcta aacttcatg acttgcgtt 
      cggttcgta ccatgggat ggtaaagct aaagattat 
      ctcctaatc tggcccatc aaatacccc aagggttgt 
      tggtcattt gtgggtcgt tttggtggc gcccagatt 
      gtgagtcta caaccacca gacgccaca agcccaaat 
      tgtcttggt actgaaggg agggccgtc catgaacta 
      atgcacgcc ttgggtgtg ttccacgaa cagtcacga 
      tgggacaga gacaagttc gttaaaatc cacatggac 
      aatatcata ccacagtac aaaaccaac tttgataag 
      caaagcctt gaaaatacg acttacagc ttcgagtat 
      gactatgat tccataatg cattacggg aaatacttt 
      ttcagtaaa ggtaaaaac aaacctaca attacagca 
      aagcaacca ggaatcaaa aggcttggt cagaggaag 
      gctctttcc aagggagac tgtttgaaa ctcaacgat 
      ctctatggc tgtttcgac aaccctcga cttcgtcgc 
      aaatactac acgttatgt caatttatg ggtatttaa
    mRNA sequence
    • >TC002341 RA
      ttatcgatt cgattccaa atcccgata aatctccgc 
      gatcatccc accaccaca aaacccacc tggcgtcca 
      aatccagct tcttaaata tgctaatga cgatttaat 
      tgacaccag ccgagcacc gctatttgc accatcaaa 
      cacttcaat catgctgct tcagtgcgc gactttcag 
      cagtcgaag atgcggcgg ccaattttc gtgttattt 
      tttctgcta catctgaaa aatgccaga gttatcctt 
      ccgacggta cccgatgtc atgcatcca cccgtaccc 
      gacgatgct aaagtcgac cgagatatg gcttttatg 
      atggaagac cccgatccc gaagtcact cccggactg 
      ttccaaggg gacatggct atgaccaac gagatctac 
      aactactgg agggtaggg atacggtgg gacgtgttt 
      ccggaaaaa ctatgggtc aatgggact gttccatat 
      gtcataagc cccatatat gaaccatcg gaccaagtg 
      acaatttta aaagccatc catattcta aacttcatg 
      acttgcgtt cggttcgta ccatgggat ggtaaagct 
      aaagattat ctcctaatc tggcccatc aaatacccc 
      aagggttgt tggtcattt gtgggtcgt tttggtggc 
      gcccagatt gtgagtcta caaccacca gacgccaca 
      agcccaaat tgtcttggt actgaaggg agggccgtc 
      catgaacta atgcacgcc ttgggtgtg ttccacgaa 
      cagtcacga tgggacaga gacaagttc gttaaaatc 
      cacatggac aatatcata ccacagtac aaaaccaac 
      tttgataag caaagcctt gaaaatacg acttacagc 
      ttcgagtat gactatgat tccataatg cattacggg 
      aaatacttt ttcagtaaa ggtaaaaac aaacctaca 
      attacagca aagcaacca ggaatcaaa aggcttggt 
      cagaggaag gctctttcc aagggagac tgtttgaaa 
      ctcaacgat ctctatggc tgtttcgac aaccctcga 
      cttcgtcgc aaatactac acgttatgt caatttatg 
      ggtatttaa tttatttaa ataaaatta attaatctt 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY