Status
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Gene information
General information
TC002358 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 33265..39449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002358
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06702

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002358 RA
      MNKGKISFG KISTNFGEN AEKIEASGS IGTFGPPKA 
      MELLEDDSK EREKMQEVM GISSFGKKA KNFDVQEMM 
      EQVKATARE ITKNPELSE QASEESEDD DDLIGPPIP 
      PDLNSGGPQ SETATKSQS SDSEEEEEP KKLFIPCTS 
      EVVMTHGTK AVTAVSVDP SGARLASGS VDYDVSFWD 
      FAGMDASMR SFRTLQPAE NHPIRYLNY SGTGDLLLV 
      ISGASQAKV LDRDGFEQL ETVKGDMYI TDQAQTKGH 
      TAGLLAGCW NPLIKEEFL TTSADGTVR TWDFYEGGK 
      NHKSIIKCR AQNGLKVSP TAVTYGRDG KVIACGCAD 
      GSLQLWDFR KSSVAPASQ LRKAHLPGE VTSIRFSNV 
      GNNLLTRSC DETMKLWDV RNLKKSLFE IGDLFTRYD 
      TTDAIFSPN DAILATGVS LRKGESSGH IFFYDATDF 
      SLIHKILIT NSHTIKLLW HPKLNQIFV GTGNGIIKC 
      FYDQKKSLR GATLCAVKV HRKAQHSEV VSTQQVITP 
      HALPLFRQE RRKTSRKQM EKDRLDPVK SRRPDLPIT 
      SGQGGRVAS SGGTLSSYV IRNLGLSKR VDDDQDPRE 
      AILKYAKEA AENPYWITP AYAKTQPEV VAKGKAPLE 
      EPPEKKTKK
    CDS sequence
    • >TC002358 RA
      atgaacaag gggaagatc agtttcggc aaaatcagc 
      acgaacttc ggcgaaaat gcggaaaaa atcgaagcc 
      agcggcagc atcggcacg ttcgggccc cccaaagcg 
      atggagctc ctcgaagac gacagcaaa gaacgcgag 
      aaaatgcag gaggttatg ggcatcagt tcgtttggc 
      aaaaaggcc aaaaacttc gacgttcag gaaatgatg 
      gagcaggtc aaagccacg gctcgggaa atcaccaaa 
      aaccctgag ctttctgag caagcttcc gaagagtcg 
      gaggacgac gacgacttg atcgggccc ccaatcccg 
      cccgatttg aactcaggg gggccccag tccgaaacc 
      gcgaccaaa tcccagtca agcgatagt gaagaggag 
      gaggaaccg aagaaattg ttcattccg tgcaccagc 
      gaggtggtg atgacacac ggcactaaa gcggtgact 
      gccgttagc gtggacccg agtggggcc cgtctggca 
      tcgggctcc gttgactac gatgtcagt ttttgggac 
      tttgccgga atggatgcc agtatgcga agtttccgg 
      acgctacaa ccggccgaa aatcaccca atcaggtat 
      ttgaactat tcaggaacc ggtgattta ctcctggtt 
      atttcgggg gcaagccag gccaaagtc ctggacagg 
      gacggcttc gagcaattg gagaccgtc aagggcgac 
      atgtacata acagatcag gcccagacc aagggtcac 
      acggcgggg ctcctcgcc ggttgctgg aaccccctc 
      attaaggag gagttcctc acaacgagc gcagatggc 
      acagtccgg acctgggat ttttacgaa gggggcaaa 
      aaccacaaa agcataatt aaatgtcgc gcccagaac 
      ggcctaaag gtctcaccc acggctgtg acatacgga 
      cgcgatggt aaagtcata gcctgtggg tgcgcagat 
      gggtctctc cagttatgg gactttagg aaaagttcc 
      gtcgcaccg gcaagtcaa ttgcgcaaa gcgcatctt 
      ccgggcgaa gtgacaagt atccggttt tcgaacgtt 
      ggtaataat ttattgaca cggagttgc gacgaaaca 
      atgaagttg tgggacgtc cggaatttg aaaaaatca 
      ctcttcgag attggcgat ttgttcacg cgttacgac 
      acaaccgac gcgattttc agccccaat gacgcaatc 
      ttagccacc ggagtgtcg ctccggaag ggcgaaagc 
      tccgggcat atttttttc tatgacgcg actgatttc 
      agcctaatt cacaaaatt ctgattacg aattcgcac 
      acgatcaag cttttgtgg cacccgaag ctgaatcag 
      attttcgtg gggacgggc aatggcata attaagtgc 
      ttctacgac cagaagaag agtctccgg ggggccacg 
      ctgtgcgcg gttaaggtg caccgcaag gcgcagcat 
      tcggaggtt gtgagcacg cagcaggtt ataaccccg 
      cacgcgttg ccgctgttc aggcaagag aggcggaag 
      acgagtcgg aagcagatg gagaaggat cggttggat 
      ccggttaag tcgcgacga cccgacttg ccgatcacg 
      tcggggcaa ggggggcgc gtggcgtcc agtgggggc 
      acgttgagt agttacgtt attcggaat ttgggcttg 
      agtaagaga gtggatgat gatcaggat ccgagagag 
      gcgattttg aagtatgca aaggaagcg gcggaaaat 
      ccgtattgg attacaccg gcctatgcc aagacgcaa 
      ccggaggtt gtggccaag gggaaggcg ccgttggag 
      gagccgccg gagaagaaa accaaaaag tga
    mRNA sequence
    • >TC002358 RA
      caagtgcaa tctattttg tgttaaagg gtttgttgt 
      ttgttaatt ttttggtag aaaatattt ggttgcgta 
      aaggcccgg tcattagtt tttgttttg acagctgtg 
      ccaaaacat aacctcaaa caaaatcga atttttatg 
      ccgaaaata tgtgttaaa ctgcgacaa tgaacaagg 
      ggaagatca gtttcggca aaatcagca cgaacttcg 
      gcgaaaatg cggaaaaaa tcgaagcca gcggcagca 
      tcggcacgt tcgggcccc ccaaagcga tggagctcc 
      tcgaagacg acagcaaag aacgcgaga aaatgcagg 
      aggttatgg gcatcagtt cgtttggca aaaaggcca 
      aaaacttcg acgttcagg aaatgatgg agcaggtca 
      aagccacgg ctcgggaaa tcaccaaaa accctgagc 
      tttctgagc aagcttccg aagagtcgg aggacgacg 
      acgacttga tcgggcccc caatcccgc ccgatttga 
      actcagggg ggccccagt ccgaaaccg cgaccaaat 
      cccagtcaa gcgatagtg aagaggagg aggaaccga 
      agaaattgt tcattccgt gcaccagcg aggtggtga 
      tgacacacg gcactaaag cggtgactg ccgttagcg 
      tggacccga gtggggccc gtctggcat cgggctccg 
      ttgactacg atgtcagtt tttgggact ttgccggaa 
      tggatgcca gtatgcgaa gtttccgga cgctacaac 
      cggccgaaa atcacccaa tcaggtatt tgaactatt 
      caggaaccg gtgatttac tcctggtta tttcggggg 
      caagccagg ccaaagtcc tggacaggg acggcttcg 
      agcaattgg agaccgtca agggcgaca tgtacataa 
      cagatcagg cccagacca agggtcaca cggcggggc 
      tcctcgccg gttgctgga accccctca ttaaggagg 
      agttcctca caacgagcg cagatggca cagtccgga 
      cctgggatt tttacgaag ggggcaaaa accacaaaa 
      gcataatta aatgtcgcg cccagaacg gcctaaagg 
      tctcaccca cggctgtga catacggac gcgatggta 
      aagtcatag cctgtgggt gcgcagatg ggtctctcc 
      agttatggg actttagga aaagttccg tcgcaccgg 
      caagtcaat tgcgcaaag cgcatcttc cgggcgaag 
      tgacaagta tccggtttt cgaacgttg gtaataatt 
      tattgacac ggagttgcg acgaaacaa tgaagttgt 
      gggacgtcc ggaatttga aaaaatcac tcttcgaga 
      ttggcgatt tgttcacgc gttacgaca caaccgacg 
      cgattttca gccccaatg acgcaatct tagccaccg 
      gagtgtcgc tccggaagg gcgaaagct ccgggcata 
      tttttttct atgacgcga ctgatttca gcctaattc 
      acaaaattc tgattacga attcgcaca cgatcaagc 
      ttttgtggc acccgaagc tgaatcaga ttttcgtgg 
      ggacgggca atggcataa ttaagtgct tctacgacc 
      agaagaaga gtctccggg gggccacgc tgtgcgcgg 
      ttaaggtgc accgcaagg cgcagcatt cggaggttg 
      tgagcacgc agcaggtta taaccccgc acgcgttgc 
      cgctgttca ggcaagaga ggcggaaga cgagtcgga 
      agcagatgg agaaggatc ggttggatc cggttaagt 
      cgcgacgac ccgacttgc cgatcacgt cggggcaag 
      gggggcgcg tggcgtcca gtgggggca cgttgagta 
      gttacgtta ttcggaatt tgggcttga gtaagagag 
      tggatgatg atcaggatc cgagagagg cgattttga 
      agtatgcaa aggaagcgg cggaaaatc cgtattgga 
      ttacaccgg cctatgcca agacgcaac cggaggttg 
      tggccaagg ggaaggcgc cgttggagg agccgccgg 
      agaagaaaa ccaaaaagt gaaaagttt tattgtata 
      cattagtat agaaataaa ttttaaaac agtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY