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Gene information
General information
TC001405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451057.1: 2955..7679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001405
    iBeetle screen iB_03240

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06692

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    remarks: eggs lost, adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    remarks: eggs lost,
    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
    8 days later, due to lost eggs in day 9

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001405 RA
      MAIQMTQEQ FQQLLLQIQ GMAAQAAPA PAPGAPRAN 
      PRHFTQCVA RFDGTRSHA AVEEFITAA TIFKEVEGI 
      EDAEALAGL PLLLMKEAN QWWSGVKNN VDTWDAAMQ 
      LIRNAFAPR RPNYRLFLE IFGEPQPEG MPTDRYVTI 
      QRDRLAQMT RHLDEEWQI DVVYGLLRH KIRDKIPRS 
      DIGSFNELL NRARAIEEN EKESTAGPV KSQKTNGTT 
      TVKANGGKN RARCSFCRA FGHDVGECR KLKTNTTSG 
      PPSAPNAIV PRVSTDIRC YGCQRPGVF RRNCPDCNP 
      PSAPVSAPT TDPTGFCAI NTSIQPRRR PTVNIEILG 
      ITGTALLDT AAGNSVASI SLYQHLLRQ GCTPRKEKV 
      EATLADGGT RLMEVPTVT TNVKLAGKI TKTTFIVLS 
      TSEDTKTLL GADFIEDSE VIIDLKHQQ FYFEQNPNE 
      KVAFANDCA TPTDEDIRT VFQATKIEF LSPLDPEYI 
      ANGYGPPPP DNSSMIPSV ITPGGTWNT YQAPGGADY 
      MLQDANEML MHYDQDHDE YQTDLFATI PYKTEICSL 
      ELRPTEGQF LTTTEKHEI NRLVKEYRD LFAEFGPPT 
      PYATHRINT GDSLPVAVS PYRLTCEKR KVLQMEIER 
      LLQQGIIEE CDSAWASPV VMVPKANGT IRLCVDYRK 
      LNAVTKPDV YPLPRLDDL LHATGKIGC ITTLDLQAG 
      YWQIQVEPG DRDKTSFIC PFGLYRFTR MPFGLRNAP 
      ASFQRLMDK FKTGIPDVP ILAYLDDLI IISPDGHTH 
      IRHLRSTFD QLRRFQLRI NRNKCLIGC SEVRYLGHR 
      ISPSGIAPD EKKVEAIKQ IPPPRNLKQ LQSFLQTCS 
      WFRRFIDQF ASVARPLSE LTKKNASWK WGNAQDEAF 
      TTLKEKLTT APILRAADP TQPFILRTD SSAYALGAA 
      LLQGEGPDE RPVEYASRL LTSSERNYS TTEREALAI 
      VWAISKFRG YVGENSTTV ITDHQALRW LMSLKTPTG 
      RLARWALQL QPYNLVIEY TPGKANTIA DFLSRPATE 
      ESSMICTVQ IDLPARSDA DIRNEQLKD PEVKKILDE 
      LMSSNPEIA QPWSSRGYV TNRGVLYRY SQADDYEEA 
      QLVVPHHER LHIMKQHHD SPTGGHYGV DKTYSKIAC 
      RYYWPGMRR DVSDYLRTC IACQRYKAS NLKPPGLLQ 
      TPWKIHALG GWNYSLLLM QLPRCALGL FLMTCAFDM 
      AYLDNGSQF VSAIMQKLT FCLGISQSF TPVYHPEAN 
      PVERKNRDL KTQLAIQIG DNPHSTWPE KLPTIRFAM 
      NTTPCSSTS VTPAFLMFG RELRTIDDV TNDLRHIVE 
      SETFVAEAT PKLLLLSDT WKKARETHE DKQDHRKEA 
      ADRTRRTAP EFQPGDLVL VQLHTLSSS AKEFSSKLA 
      PKRDGPYVI LRRHGPTSY EIAAQDQPS QALGTYHSS 
      QLTPYRGLE KEMPPPVVP IRRRGRPRK HNVQNQ
    CDS sequence
    • >TC001405 RA
      atggcgatc cagatgacg caggagcaa tttcaacaa 
      ttgctgctg caaatccaa ggaatggcg gcacaagca 
      gcacctgca ccagcacct ggggcacct cgcgctaac 
      cccaggcat ttcacacaa tgtgtggca agattcgat 
      ggcaccagg tcgcacgca gcagttgag gaattcatc 
      acagcggcc accatattc aaagaagtg gaaggcata 
      gaagatgcc gaagcactt gcaggtctt cctctgctt 
      ctgatgaaa gaagccaat caatggtgg agtggtgta 
      aagaataac gtcgacact tgggatgcc gccatgcag 
      ctaatccgg aacgccttc gcccctcgg cgcccgaac 
      tatcgacta tttctggaa atcttcggt gaaccccaa 
      ccagaagga atgcccacg gacagatat gtaacgatc 
      caacgggac cgcctcgca cagatgaca cgccatctt 
      gacgaagaa tggcaaatt gacgttgtg tacggccta 
      ttaagacat aaaattcgc gacaaaatt ccgcgcagt 
      gatattgga tcatttaac gaacttttg aatcgcgcg 
      cgagcaatc gaagaaaac gaaaaagaa tcgaccgcg 
      ggcccggta aaatcccaa aaaacgaat gggaccacg 
      accgttaaa gcaaacggc gggaaaaat cgcgcacgg 
      tgtagtttc tgtagagca tttggacat gatgttggt 
      gaatgtaga aaactaaag accaacacc acttccgga 
      ccaccttct gcaccaaat gcaatagtg ccgcgagtg 
      agtacggac attcggtgt tacggatgc caaaggccg 
      ggggtgttc cgacgcaat tgtccggat tgcaatcca 
      cctagtgct ccagtaagt gctccaacc acggatcca 
      actggattc tgtgcaata aacacaagc attcaacct 
      cggcgtcgg ccaacggtt aatatagaa atcttgggc 
      atcacggga accgcattg ttggacacc gccgccggc 
      aatagtgtg gcaagcata agcctgtat caacacctc 
      ctccgacaa ggttgtact ccacgtaaa gaaaaagtg 
      gaagcaact cttgcggat ggtggaacc cgtctgatg 
      gaagtcccc actgtaaca actaatgta aagctggca 
      ggcaaaatc acaaaaacc acttttatc gtgctctca 
      acctcagag gatacaaaa actttgttg ggagccgac 
      tttattgaa gacagtgaa gtgatcatc gacctaaag 
      catcagcag ttttacttc gaacaaaat ccaaatgaa 
      aaggttgcc ttcgctaac gattgcgcc actcccacc 
      gatgaagac attcgtacc gtgttccaa gcaacaaag 
      atagaattt ctgtcaccc ttggacccc gaatatatt 
      gctaatgga tacggccct ccaccaccc gataattca 
      agtatgata ccgtccgta attactccc ggaggtacg 
      tggaacact tatcaagct cccggtggc gccgactac 
      atgctacaa gatgccaac gagatgctg atgcattac 
      gaccaggat cacgatgaa taccagacc gatctattt 
      gcgacgatc ccttacaaa acggaaatt tgttcattg 
      gagcttcgc cctacagaa ggtcagttt ttaaccacc 
      acggagaag catgaaatc aaccggtta gtgaaggaa 
      tacagagac ttatttgcg gaatttggt ccaccaacc 
      ccatacgcc acccatcga attaatacc ggtgacagc 
      ctccctgta gcagtatct ccatatcgc ctgacatgt 
      gagaaacga aaggtcctc caaatggaa atagagcga 
      ctccttcaa cagggtatc atcgaagaa tgtgatagt 
      gcgtgggcg tctcccgtg gtaatggta cccaaagcc 
      aatgggacc attaggcta tgtgtggat taccgtaaa 
      ctaaatgct gtaacaaaa ccagatgtc tatccactt 
      cctagactg gacgatctg ttacacgca actggaaag 
      attggttgc attacaacg ttggaccta caggccggc 
      tattggcag atccaggtg gaaccggga gatagggat 
      aaaacatct tttatctgc ccttttgga ctatatcgc 
      ttcacacgg atgccattc ggtctacgc aatgcaccg 
      gcttcgttc caacgttta atggacaaa tttaaaacc 
      ggcattccc gacgttccc atactggca tacctggat 
      gatctcatt ataatctcc cccgatggt catacacat 
      attcgccac ttgagaagt acttttgac cagttacga 
      cgctttcaa ctacgcata aacaggaac aagtgcttg 
      atcggatgt tcggaggta cgatatctt ggacatcgt 
      atctctccc tctggcatc gccccagat gaaaagaag 
      gtggaagct attaaacaa ataccacct ccacgaaac 
      ctgaagcag ctgcagtcc ttcttacaa acctgctct 
      tggtttcgc cgatttatt gatcagttc gcatccgtg 
      gcacgtcca ctaagtgaa ctaaccaaa aagaacgct 
      tcttggaaa tggggaaac gcacaagac gaggcattc 
      accacttta aaggaaaag ttgacaaca gctcctata 
      ttacgagca gccgatcca acccaaccg tttatctta 
      cgaaccgat agcagtgcg tatgcactc ggtgcggct 
      cttctccag ggggagggc cccgacgag agacccgtt 
      gaatacgct agccgcttg ttaacttca tcggaacgc 
      aactattca accacggaa cgggaagcc ctcgccatc 
      gtatgggcc attagcaaa ttcagagga tatgtggga 
      gaaaactca acaaccgta atcaccgat catcaagct 
      ctgcgatgg ctcatgtcg ctaaaaact cctacggga 
      cggcttgct cgatgggct cttcagctc cagccatac 
      aatctggta atcgaatat acaccagga aaagcaaat 
      actattgcc gactttttg tcacgacca gcaactgaa 
      gaatctagt atgatctgc acagtccaa attgattta 
      ccggctcgc tcggacgcc gacattcgc aacgaacaa 
      ttaaaggac ccagaagta aagaagatt ctcgacgaa 
      ttaatgtcc tcaaatcct gaaatcgct cagccctgg 
      tcttctcgt ggatacgtc acgaatcga ggtgtgttg 
      taccgctac tcccaagcc gatgattat gaagaggcc 
      caactcgta gtaccacat catgaacgt ctccatatt 
      atgaaacaa caccatgac tcccctacc ggcggccat 
      tacggcgtt gacaaaacc tacagtaag atcgcctgc 
      cgctattac tggcctggt atgagaaga gatgtttcc 
      gactacctg cgcacttgt attgcttgc cagcgatac 
      aaggcctct aacctgaaa cccccagga ctcttgcaa 
      accccgtgg aagatacat gcactaggt gggtggaac 
      tattccctc ttgttgatg caactgccg agatgtgcg 
      cttggactc ttcttaatg acgtgtgct ttcgatatg 
      gcctacctc gacaacgga tcacaattt gtgagcgcc 
      ataatgcag aaactcacc ttctgctta ggaataagc 
      caatccttc actccagtt tatcatccg gaggctaac 
      ccagtagaa cggaaaaat cgggacctt aaaacgcaa 
      ctcgctatc caaataggc gacaatcca cactccact 
      tggccagaa aagcttccg accatacgc tttgctatg 
      aatacaacg ccctgttcc agcactagt gtcactcct 
      gcattcctg atgtttgga cgagaatta cgaaccatc 
      gacgacgtc actaatgat ttacgacac atagttgaa 
      agtgagacc tttgtggcc gaagccaca ccgaaactt 
      ttactgttg agcgatacg tggaaaaag gctagagaa 
      acacatgaa gacaaacaa gatcatcgg aaggaagcc 
      gctgatcgc acccgaaga acagccccc gagtttcaa 
      cccggagat ttggttttg gtccaactc catactctc 
      agcagtagc gccaaagaa tttagttcg aaactggcc 
      ccaaaacgg gatggtccc tacgtaatc ctacgacgg 
      catggcccc actagttat gaaattgca gcccaggat 
      caaccaagt caagcacta gggacatac cacagctct 
      caactcaca ccataccgc ggcctggaa aaggaaatg 
      ccaccccct gttgtgcct attcgccgg cggggacga 
      ccccgaaaa cataacgtc cagaaccag tga
    mRNA sequence
    • >TC001405 RA
      cacgtttga acgagatgg cgatccaga tgacgcagg 
      agcaatttc aacaattgc tgctgcaaa tccaaggaa 
      tggcggcac aagcagcac ctgcaccag cacctgggg 
      cacctcgcg ctaacccca ggcatttca cacaatgtg 
      tggcaagat tcgatggca ccaggtcgc acgcagcag 
      ttgaggaat tcatcacag cggccacca tattcaaag 
      aagtggaag gcatagaag atgccgaag cacttgcag 
      gtcttcctc tgcttctga tgaaagaag ccaatcaat 
      ggtggagtg gtgtaaaga ataacgtcg acacttggg 
      atgccgcca tgcagctaa tccggaacg ccttcgccc 
      ctcggcgcc cgaactatc gactatttc tggaaatct 
      tcggtgaac cccaaccag aaggaatgc ccacggaca 
      gatatgtaa cgatccaac gggaccgcc tcgcacaga 
      tgacacgcc atcttgacg aagaatggc aaattgacg 
      ttgtgtacg gcctattaa gacataaaa ttcgcgaca 
      aaattccgc gcagtgata ttggatcat ttaacgaac 
      ttttgaatc gcgcgcgag caatcgaag aaaacgaaa 
      aagaatcga ccgcgggcc cggtaaaat cccaaaaaa 
      cgaatggga ccacgaccg ttaaagcaa acggcggga 
      aaaatcgcg cacggtgta gtttctgta gagcatttg 
      gacatgatg ttggtgaat gtagaaaac taaagacca 
      acaccactt ccggaccac cttctgcac caaatgcaa 
      tagtgccgc gagtgagta cggacattc ggtgttacg 
      gatgccaaa ggccggggg tgttccgac gcaattgtc 
      cggattgca atccaccta gtgctccag taagtgctc 
      caaccacgg atccaactg gattctgtg caataaaca 
      caagcattc aacctcggc gtcggccaa cggttaata 
      tagaaatct tgggcatca cgggaaccg cattgttgg 
      acaccgccg ccggcaata gtgtggcaa gcataagcc 
      tgtatcaac acctcctcc gacaaggtt gtactccac 
      gtaaagaaa aagtggaag caactcttg cggatggtg 
      gaacccgtc tgatggaag tccccactg taacaacta 
      atgtaaagc tggcaggca aaatcacaa aaaccactt 
      ttatcgtgc tctcaacct cagaggata caaaaactt 
      tgttgggag ccgacttta ttgaagaca gtgaagtga 
      tcatcgacc taaagcatc agcagtttt acttcgaac 
      aaaatccaa atgaaaagg ttgccttcg ctaacgatt 
      gcgccactc ccaccgatg aagacattc gtaccgtgt 
      tccaagcaa caaagatag aatttctgt cacccttgg 
      accccgaat atattgcta atggatacg gccctccac 
      cacccgata attcaagta tgataccgt ccgtaatta 
      ctcccggag gtacgtgga acacttatc aagctcccg 
      gtggcgccg actacatgc tacaagatg ccaacgaga 
      tgctgatgc attacgacc aggatcacg atgaatacc 
      agaccgatc tatttgcga cgatccctt acaaaacgg 
      aaatttgtt cattggagc ttcgcccta cagaaggtc 
      agtttttaa ccaccacgg agaagcatg aaatcaacc 
      ggttagtga aggaataca gagacttat ttgcggaat 
      ttggtccac caaccccat acgccaccc atcgaatta 
      ataccggtg acagcctcc ctgtagcag tatctccat 
      atcgcctga catgtgaga aacgaaagg tcctccaaa 
      tggaaatag agcgactcc ttcaacagg gtatcatcg 
      aagaatgtg atagtgcgt gggcgtctc ccgtggtaa 
      tggtaccca aagccaatg ggaccatta ggctatgtg 
      tggattacc gtaaactaa atgctgtaa caaaaccag 
      atgtctatc cacttccta gactggacg atctgttac 
      acgcaactg gaaagattg gttgcatta caacgttgg 
      acctacagg ccggctatt ggcagatcc aggtggaac 
      cgggagata gggataaaa catctttta tctgccctt 
      ttggactat atcgcttca cacggatgc cattcggtc 
      tacgcaatg caccggctt cgttccaac gtttaatgg 
      acaaattta aaaccggca ttcccgacg ttcccatac 
      tggcatacc tggatgatc tcattataa tctcccccg 
      atggtcata cacatattc gccacttga gaagtactt 
      ttgaccagt tacgacgct ttcaactac gcataaaca 
      ggaacaagt gcttgatcg gatgttcgg aggtacgat 
      atcttggac atcgtatct ctccctctg gcatcgccc 
      cagatgaaa agaaggtgg aagctatta aacaaatac 
      cacctccac gaaacctga agcagctgc agtccttct 
      tacaaacct gctcttggt ttcgccgat ttattgatc 
      agttcgcat ccgtggcac gtccactaa gtgaactaa 
      ccaaaaaga acgcttctt ggaaatggg gaaacgcac 
      aagacgagg cattcacca ctttaaagg aaaagttga 
      caacagctc ctatattac gagcagccg atccaaccc 
      aaccgttta tcttacgaa ccgatagca gtgcgtatg 
      cactcggtg cggctcttc tccaggggg agggccccg 
      acgagagac ccgttgaat acgctagcc gcttgttaa 
      cttcatcgg aacgcaact attcaacca cggaacggg 
      aagccctcg ccatcgtat gggccatta gcaaattca 
      gaggatatg tgggagaaa actcaacaa ccgtaatca 
      ccgatcatc aagctctgc gatggctca tgtcgctaa 
      aaactccta cgggacggc ttgctcgat gggctcttc 
      agctccagc catacaatc tggtaatcg aatatacac 
      caggaaaag caaatacta ttgccgact ttttgtcac 
      gaccagcaa ctgaagaat ctagtatga tctgcacag 
      tccaaattg atttaccgg ctcgctcgg acgccgaca 
      ttcgcaacg aacaattaa aggacccag aagtaaaga 
      agattctcg acgaattaa tgtcctcaa atcctgaaa 
      tcgctcagc cctggtctt ctcgtggat acgtcacga 
      atcgaggtg tgttgtacc gctactccc aagccgatg 
      attatgaag aggcccaac tcgtagtac cacatcatg 
      aacgtctcc atattatga aacaacacc atgactccc 
      ctaccggcg gccattacg gcgttgaca aaacctaca 
      gtaagatcg cctgccgct attactggc ctggtatga 
      gaagagatg tttccgact acctgcgca cttgtattg 
      cttgccagc gatacaagg cctctaacc tgaaacccc 
      caggactct tgcaaaccc cgtggaaga tacatgcac 
      taggtgggt ggaactatt ccctcttgt tgatgcaac 
      tgccgagat gtgcgcttg gactcttct taatgacgt 
      gtgctttcg atatggcct acctcgaca acggatcac 
      aatttgtga gcgccataa tgcagaaac tcaccttct 
      gcttaggaa taagccaat ccttcactc cagtttatc 
      atccggagg ctaacccag tagaacgga aaaatcggg 
      accttaaaa cgcaactcg ctatccaaa taggcgaca 
      atccacact ccacttggc cagaaaagc ttccgacca 
      tacgctttg ctatgaata caacgccct gttccagca 
      ctagtgtca ctcctgcat tcctgatgt ttggacgag 
      aattacgaa ccatcgacg acgtcacta atgatttac 
      gacacatag ttgaaagtg agacctttg tggccgaag 
      ccacaccga aacttttac tgttgagcg atacgtgga 
      aaaaggcta gagaaacac atgaagaca aacaagatc 
      atcggaagg aagccgctg atcgcaccc gaagaacag 
      cccccgagt ttcaacccg gagatttgg ttttggtcc 
      aactccata ctctcagca gtagcgcca aagaattta 
      gttcgaaac tggccccaa aacgggatg gtccctacg 
      taatcctac gacggcatg gccccacta gttatgaaa 
      ttgcagccc aggatcaac caagtcaag cactaggga 
      cataccaca gctctcaac tcacaccat accgcggcc 
      tggaaaagg aaatgccac cccctgttg tgcctattc 
      gccggcggg gacgacccc gaaaacata acgtccaga 
      accagtgat cgggacgct tcacatggt tctgagggg 
      gagaactgt agcaataat tagtacggg tagttcata 
      agcgctcca ccaataccc ttttggtag cgccacttg 
      caccagcgg tcacagccc cactacccc aactagccc 
      tttttgcgc aggcaccgc cttccgcaa acccaataa 
      aaggacgat ctctcccct ggactcaac agattcagt 
      cgagcatcc ttcggggtc caactgaaa acaacttgc 
      gcctatcta ggattttct ttggccttg ttcgcttaa 
      tgttataca aatacacgt tttaacaaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY