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Gene information
General information
TC012898 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18809081..18833649
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012898
    iBeetle screen iB_06690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012898 RA
      MEVTSEDEL LFHKLKSFC EINEQFCIA FKLTEKRTG 
      LNRHQKNDQ HLHYIAQHV ITIAVYFQS VVTHFSEFL 
      PKIVSIATV ELGQHRANC VLLGRIIRI RNDLHCYAT 
      KYFESNPSP FEDESYPVS KKRKMSQIT ISELDLVES 
      CYALLKSDP QFYGKKWNW SEFITKYLN HESPEIQWI 
      ACHCISVLT HMSENQLRD LILSKLPQE THEKFSCIY 
      NNSLTLGKT GSEIVALDQ HPVIGISKE TQDVVDISG 
      VYIPKLKSS VTPHHNLIM VNSTRDNLR KVAFGLSSK 
      KAICLQGPV GSGKTSLVE HIANKTGRK LGENFVKVQ 
      LGDQTDSKM LLGTYRCTD IPGEFVWQP GVVTQAVIE 
      GNWVLLEDI DSASMDIAS VLSSLLENG SLTVPGYRD 
      SLPITPGFQ LFFTQRLVS TITGHHKKH SNAMTLLEK 
      HLLQINIDP LTSAELEEI LITQHPQFA TIADRMIKV 
      FHLFTTNTD LPKSSRLIS TRDFFKWLS RAAIDFDIK 
      SQSSALKVL QDAIDVFCC SYAKPDEAL GLAKQISTH 
      LGIINQKAE FFFKSYKPV IKLSASHLE VGRAVMARN 
      NDVLSLRQS YYFTRPSTV LLERVMCCV KLQEPVLLV 
      GETGTGKTS TVQYLANAI GQKLVVINM NQQSDSADL 
      LGGYKPVDL KFVITPIRK EFEALFCEF FQVEPNRTF 
      LGHIARCYN EQRWSDLVK LMTKSYDAA ISRLKQPSP 
      KKHLLARWQ SLGEKLHKL TLQLKQKSA LAFAFIEGS 
      LVRAVEEGH WVLLDEINL ANAETLECL SGLLEGAEG 
      SLCLLERGD KAPVERHPN FTLFACMNP STDVGKKDL 
      PAGLRNRFT EFYVDELVE RNDLVLLVG SYLEALSLK 
      EGELERIVE FYLRVRKET EVSLVDGLG HKPHFSLRS 
      LCRALMITG RNPCGMVKK SLYEAFCLS FLTQLDEQS 
      YGIVEKLIA KYLVGDDKA LKAVLKQPI PTPKSNSDD 
      YVQFEGYWV RKGDLECLV PDDYVLTES VRKNLKDLV 
      RIVSIGGLP VLLQGDTSV GKTSLIAYL AKSSGNKCV 
      RINNHEHTD LQEYIGSYV ADCEGKLVF REGLLVEAM 
      RKGHWIILD ELNLAPSDV LEALNRVLD DNRELFIPE 
      TQETVKADP NFMLFATQN PPGSYGGRK MLSRAFRNR 
      FVELHFNEI PPKELEFIL HKRCQMAPS YAKKMINVM 
      TDLQTRRRG SAAFAGKQG FITLRDLFR WGERYRLAQ 
      NTQKLYDWD QHLADEGYL VLAGRVRKS DEKKEIITV 
      LQKHLKREV NPHNLFTLS EKTSVVTRD ILTKIYQNR 
      DKYKNIVWT YNMRQLAVL VAKAFHFKE PVLLVGETG 
      GGKTTVCKL IAELNQQEL ISVNCHMHT ESSDFIGGL 
      RPVRDHVDD DVNKLFEWV DGPLIKAML KGNVFLADE 
      ISLADDSVL ERLNSLLEP ERTLLLAEK GADINNPNN 
      SELIVADPN FYFIGTMNP GGDFGKKEL SPALRNRFT 
      EIWCESCTD RDDLIAIIE QNVKEGISF GNQQDGSSG 
      IGKNIMDFV EWFRGTEIG KRCTVSIRD ILTWVNFIN 
      NCAEKIGVY ESYLHGLHL TFLDALGTG ITGTENLNN 
      LKKFKHCCE LFIKKQLNS STLPHVFSP NLLIEESDN 
      SFGIKPFYI ETGNEPPTP NSFTFSAPT TTYNTLRLL 
      RGMQLNKAI LLEGSPGVG KTSLVTALA KLTRHKIFR 
      VNLSDQTDI SDLFGADLP VEGGSGGQF SWRDGPLLQ 
      ALKQGHWIL LDELNLASQ SVLEGLNAC LDHRGEIFI 
      PELGKTFHV RPGTRFFAC QNPLKQGGS RRGLPQSFL 
      NRFIQVYVN SLTSKDLEL ILTNQFRAI PREIIDKML 
      KFNSRVCDE LDGHSFGNK GAPWELNLR DLSRWCEAM 
      IYHFEANPS VDKKYQPES LVHLIYGDR MRTLRDKRK 
      VSEMFEEVF GRGISGDAP VLYTNSEQV WIGDVGLWR 
      NRDGVDVNV ISDEKSCLV LRTQLPVLR SLAYCVNLN 
      WMSLLVGSS AAGKSTVVR ALAHIVGKT LRTLPVTSA 
      MDTTDILGG FEQVSQVLL
    CDS sequence
    • >TC012898 RA
      atggaagtg acaagtgag gatgagttg ttgtttcac 
      aaactgaag agtttttgt gaaatcaac gaacagttt 
      tgtattgct tttaagctt acggagaag agaactggc 
      ttaaatcgg caccagaaa aacgaccaa catttgcat 
      tacatagcc caacatgtt ataactatt gcggtttat 
      ttccaaagt gttgtcaca catttttcg gaatttctt 
      cccaaaatt gtgtctatt gcaactgtt gagttgggg 
      caacatagg gccaactgc gtgttgtta ggcagaatt 
      atacgaatt agaaatgat ttgcattgc tacgccact 
      aaatatttc gagtccaac ccctcaccc tttgaggat 
      gaatcttat ccagtttca aagaagcgc aaaatgtcg 
      caaataaca atttccgaa ctagattta gtcgagtct 
      tgttacgct ttattgaaa agtgaccct caattttac 
      ggcaaaaaa tggaattgg tccgaattt atcaccaaa 
      tatttaaat catgaaagt ccagaaatc caatggatt 
      gcctgtcac tgcatttca gtcctcacc catatgagc 
      gaaaatcag ctcagagat ttgatttta tcgaaattg 
      ccacaagag actcacgaa aaattttcg tgcatttac 
      aataattca ctcactttg ggtaaaacg ggatcggaa 
      attgtggct ttggatcaa caccccgtc atcggtatt 
      agtaaagaa acgcaggac gttgttgat atttccggt 
      gtttacatt cctaaatta aaatcgtcc gtaacgcca 
      catcacaat ttgattatg gtcaattcc acgcgtgat 
      aatttgcga aaagtagct tttggttta tcttcgaag 
      aaagccatt tgtttgcaa ggcccggtc gggtcggga 
      aaaactagt ttggtggaa cacattgcg aataaaact 
      ggcagaaaa ctgggagag aattttgta aaagttcag 
      ttaggtgat caaactgat agtaagatg ttattgggg 
      acgtatcga tgcactgat attcctggg gagtttgtc 
      tggcagccg ggagttgta acacaggcg gtgattgaa 
      ggaaattgg gtcttactg gaagacatt gattcagca 
      agtatggat atagcctca gttttgtca agtttgcta 
      gaaaatggg agtctcacg gttcccgga taccgcgac 
      tctttgccg attactccc ggatttcag ctatttttc 
      actcaaaga cttgtgtct acaataacc ggccaccac 
      aaaaaacac tcaaacgca atgacttta ctcgagaaa 
      cacttgctt caaataaac atcgaccct ttgacttca 
      gccgaactt gaggaaatt ttaataaca caacatcca 
      caatttgcc acaattgcg gaccggatg attaaagtt 
      ttccattta tttacgaca aacactgac ttgcccaaa 
      agcagtcgc ttgatttca acgagagac tttttcaag 
      tggttgtca cgagctgcg attgatttt gatataaag 
      agccaaagt agtgctttg aaagtgctg caagatgca 
      atcgatgtg ttctgttgc tcgtatgcc aaaccagac 
      gaagcgctg ggtttggct aagcagatt agcactcac 
      ttggggatt attaaccag aaagcggaa tttttcttc 
      aagagttat aaaccagtt ataaagtta agtgcaagt 
      catttggaa gttggacgt gcagttatg gcacgaaac 
      aatgatgtt ttgtccttg cggcagtct tattatttc 
      actcgtcct tcgaccgtt ctccttgaa cgcgttatg 
      tgctgtgtt aaactgcaa gaaccggtt cttttggtc 
      ggtgaaacc ggaaccggt aaaacctca accgttcag 
      tatttagcg aacgcaatc ggccaaaaa ctggtcgtt 
      atcaacatg aaccagcag tcggactct gccgatttg 
      cttggcggt tacaaaccg gttgatttg aaatttgta 
      atcacccca attcgtaaa gaattcgaa gcacttttc 
      tgtgagttt ttccaagtc gagccaaac cgtacgttt 
      ctgggacac atcgctcgc tgttacaac gaacagagg 
      tggtcagac ctcgttaaa ttaatgacg aaaagttac 
      gatgcggct atttcgcgt ctgaagcaa ccctcacct 
      aaaaaacac ctcctcgcc cgttggcaa tcccttggc 
      gagaagttg cacaaactc actttgcag ctgaaacag 
      aaaagtgcc ctcgcgttc gctttcatt gaaggcagt 
      ttagtgaga gccgttgaa gaggggcac tgggtcctt 
      ttggacgag attaatcta gcgaacgcc gaaacgctc 
      gaatgtttg tcaggactt ttggaggga gcggaaggc 
      tcgctgtgt ttgctcgaa aggggcgat aaagccccg 
      gtggaacgg caccccaat tttaccctc tttgcgtgc 
      atgaaccct tcgacggac gtgggcaag aaggatctt 
      ccagcgggg ctgcggaac cgttttaca gaattttac 
      gttgatgag ttggtcgag agaaacgac ttggttttg 
      ctggttggg agttatctg gaagcgttg tctttgaag 
      gagggcgag ttggagcga attgttgag ttttatttg 
      agggtgcgg aaggagacg gaggtttcg ttggtggat 
      gggttgggg cacaagccg catttctcg ctgaggtcg 
      ctttgtcgc gccttgatg attacaggg aggaatccg 
      tgtgggatg gtcaagaag agtttgtat gaggcgttt 
      tgtttgagt tttttgacg cagttggat gagcagtcg 
      tatgggatt gttgagaag ttgattgcg aagtatttg 
      gttggagat gataaggcg ttgaaagca gttttgaag 
      cagcctatt cctacgcct aaaagtaat tctgatgat 
      tatgtccag tttgagggt tattgggtt cggaaaggg 
      gatttggag tgtttggtt cctgatgat tacgttttg 
      accgagtct gtgcgaaaa aatcttaaa gatttggtg 
      cgaattgtg tcgattggg ggactgcca gttttacta 
      cagggcgac acttccgtc ggtaaaacc agtctgatc 
      gcttacttg gccaagtca agcggtaac aaatgcgtc 
      cgcataaac aaccacgaa cacaccgat ctccaggaa 
      tacattggc tcgtacgtc gccgactgc gagggcaaa 
      ctggtcttc cgggaaggc ctcctggtg gaggcaatg 
      cgcaaaggc cattggatc atcctcgac gagctcaac 
      ctggcacct agtgacgtc ctggaggcc ctaaaccgc 
      gtcctggat gacaatcgc gaattattc attccagaa 
      acacaagaa accgtcaaa gccgacccc aatttcatg 
      ctcttcgcc acacagaat ccgcccggt tcgtacggc 
      ggacgcaaa atgctgtcg agggctttc aggaatcga 
      ttcgttgaa ctgcatttc aacgaaatt ccgccgaag 
      gagttggag tttattttg cataagcgg tgtcaaatg 
      gcaccgtct tatgccaag aagatgatt aatgtcatg 
      acggatcta cagactcgg agacggggt tcggctgcg 
      tttgccgga aagcagggt tttatcact ttgagggat 
      ttgttcaga tggggcgaa aggtatcgc ctggcccaa 
      aacacccaa aaattgtac gattgggac caacatttg 
      gcggatgaa ggctattta gtcctagcg ggaagggtc 
      aggaaaagt gacgagaaa aaagagatt atcaccgtt 
      ttgcaaaag caccttaaa cgcgaagtt aacccgcac 
      aatttattc actctgtcg gaaaaaaca tcagttgtg 
      acccgagat attttaaca aaaatttac caaaatcgc 
      gacaaatac aaaaacatt gtgtggacc tataatatg 
      agacagttg gcggttttg gtggctaaa gcgttccat 
      ttcaaagaa cctgttctc ctagtgggt gaaacagga 
      ggtggcaaa acaaccgtt tgtaaatta attgctgaa 
      ttgaatcag caggaatta atttcggtt aactgtcac 
      atgcatacc gaaagcagc gattttatt ggtggttta 
      agaccggtc agagatcat gtggacgat gatgttaac 
      aaattattt gaatgggtt gacggtcct ttaattaaa 
      gccatgtta aaaggcaac gtttttctc gccgacgaa 
      atttcgctg gctgatgat agtgtgctc gaacgtctt 
      aactctctt ctagagccg gaacggact ttattattg 
      gctgaaaaa ggggccgat atcaataat ccaaacaac 
      tcggaatta attgtcgcc gatcccaac ttttatttt 
      attgggacg atgaatcct ggaggtgat tttggcaag 
      aaagaattg tcaccagct ttgcgaaat cggtttacg 
      gagatctgg tgtgaatcg tgcactgat cgcgatgat 
      ttgattgcg ataattgaa caaaatgtc aaggaggga 
      atttctttt gggaatcaa caggatgga tcgagtggg 
      attggtaaa aatattatg gattttgtg gaatggttt 
      cgaggtact gaaattggg aaaaggtgc actgtaagt 
      attcgcgac attttaact tgggtaaac ttcatcaac 
      aactgtgct gaaaaaatc ggagtttac gaatcgtac 
      cttcacggc ttacatctc acatttctg gacgcttta 
      ggcaccgga atcaccgga acagaaaat ttaaacaac 
      ttgaaaaaa ttcaaacac tgttgcgaa ctgttcatt 
      aaaaaacaa ctaaattct tcaaccctc ccgcacgta 
      ttttcgcca aatttactg atcgaagaa tcggacaat 
      tcattcgga attaaaccc ttctacatc gaaacgggt 
      aatgagcct cccactccc aactccttc acattcagc 
      gctcccaca acgacatac aacactcta agattattg 
      agaggaatg cagttgaac aaggcgatt ttacttgag 
      ggcagtccc ggagtcggc aaaactagc cttgttaca 
      gctttggca aaactaacc cgccataaa atcttccgt 
      gtgaattta tccgaccaa accgatatt tccgacctg 
      tttggggcc gatttaccg gtggagggg ggctcaggg 
      gggcagttc tcgtggcgt gatggcccc ctgcttcag 
      gcccttaaa caagggcac tggatttta ctcgacgag 
      ttaaatttg gcctcacag tcggttctg gaaggttta 
      aatgcgtgt ttggaccac cgaggcgag atttttatc 
      cccgagttg ggcaaaacg ttccacgta agaccagga 
      acgcgtttt ttcgcctgt cagaacccc cttaaacaa 
      gggggcagc cggaggggt ttgccccag tcgtttctc 
      aatcgtttt atccaagtt tacgtcaac tcgctcact 
      tctaaagac ttggaatta attttgact aaccaattc 
      cgggccatt cctcgtgaa ataatcgac aagatgttg 
      aaatttaac tcaagggtt tgcgatgag ttggatggt 
      catagtttt gggaataaa ggcgcgcct tgggagttg 
      aacttacgc gatttgagt cgatggtgt gaggccatg 
      atttatcac tttgaagct aacccaagt gtcgataag 
      aaatatcaa ccggagagt ttggtgcac ttaatttac 
      ggggatcgc atgaggact ttgcgggat aagaggaaa 
      gtgagtgag atgtttgag gaagtgttc ggccggggc 
      atttctggt gatgcgccc gttttgtat acaaatagt 
      gaacaagtg tggattggt gatgtgggg ttgtggagg 
      aatagggac ggcgttgat gttaatgtg atcagtgac 
      gagaaaagt tgtttggtt ttgaggacg cagttgccg 
      gttttgagg agtttggcc tattgtgtt aatctcaac 
      tggatgagt ttactggtg gggagttct gcggcagga 
      aaaagcaca gtggtgcga gctttggct cacatcgtt 
      ggaaaaacg ctccgaact ttgcctgta acgagtgca 
      atggataca acggatatc ttgggagga ttcgaacag 
      gtaagtcaa gttttgctc tga
    mRNA sequence
    • >TC012898 RA
      gtgtgttgt taggttaga attgtaacc gagttgggg 
      ttcagttca gtgtgtttt gtgcagcaa agaaatgtt 
      gttgggtgg ttaaaatgg aagtgacaa gtgaggatg 
      agttgttgt ttcacaaac tgaagagtt tttgtgaaa 
      tcaacgaac agttttgta ttgctttta agcttacgg 
      agaagagaa ctggcttaa atcggcacc agaaaaacg 
      accaacatt tgcattaca tagcccaac atgttataa 
      ctattgcgg tttatttcc aaagtgttg tcacacatt 
      tttcggaat ttcttccca aaattgtgt ctattgcaa 
      ctgttgagt tggggcaac atagggcca actgcgtgt 
      tgttaggca gaattatac gaattagaa atgatttgc 
      attgctacg ccactaaat atttcgagt ccaacccct 
      caccctttg aggatgaat cttatccag tttcaaaga 
      agcgcaaaa tgtcgcaaa taacaattt ccgaactag 
      atttagtcg agtcttgtt acgctttat tgaaaagtg 
      accctcaat tttacggca aaaaatgga attggtccg 
      aatttatca ccaaatatt taaatcatg aaagtccag 
      aaatccaat ggattgcct gtcactgca tttcagtcc 
      tcacccata tgagcgaaa atcagctca gagatttga 
      ttttatcga aattgccac aagagactc acgaaaaat 
      tttcgtgca tttacaata attcactca ctttgggta 
      aaacgggat cggaaattg tggctttgg atcaacacc 
      ccgtcatcg gtattagta aagaaacgc aggacgttg 
      ttgatattt ccggtgttt acattccta aattaaaat 
      cgtccgtaa cgccacatc acaatttga ttatggtca 
      attccacgc gtgataatt tgcgaaaag tagcttttg 
      gtttatctt cgaagaaag ccatttgtt tgcaaggcc 
      cggtcgggt cgggaaaaa ctagtttgg tggaacaca 
      ttgcgaata aaactggca gaaaactgg gagagaatt 
      ttgtaaaag ttcagttag gtgatcaaa ctgatagta 
      agatgttat tggggacgt atcgatgca ctgatattc 
      ctggggagt ttgtctggc agccgggag ttgtaacac 
      aggcggtga ttgaaggaa attgggtct tactggaag 
      acattgatt cagcaagta tggatatag cctcagttt 
      tgtcaagtt tgctagaaa atgggagtc tcacggttc 
      ccggatacc gcgactctt tgccgatta ctcccggat 
      ttcagctat ttttcactc aaagacttg tgtctacaa 
      taaccggcc accacaaaa aacactcaa acgcaatga 
      ctttactcg agaaacact tgcttcaaa taaacatcg 
      accctttga cttcagccg aacttgagg aaattttaa 
      taacacaac atccacaat ttgccacaa ttgcggacc 
      ggatgatta aagttttcc atttattta cgacaaaca 
      ctgacttgc ccaaaagca gtcgcttga tttcaacga 
      gagactttt tcaagtggt tgtcacgag ctgcgattg 
      attttgata taaagagcc aaagtagtg ctttgaaag 
      tgctgcaag atgcaatcg atgtgttct gttgctcgt 
      atgccaaac cagacgaag cgctgggtt tggctaagc 
      agattagca ctcacttgg ggattatta accagaaag 
      cggaatttt tcttcaaga gttataaac cagttataa 
      agttaagtg caagtcatt tggaagttg gacgtgcag 
      ttatggcac gaaacaatg atgttttgt ccttgcggc 
      agtcttatt atttcactc gtccttcga ccgttctcc 
      ttgaacgcg ttatgtgct gtgttaaac tgcaagaac 
      cggttcttt tggtcggtg aaaccggaa ccggtaaaa 
      cctcaaccg ttcagtatt tagcgaacg caatcggcc 
      aaaaactgg tcgttatca acatgaacc agcagtcgg 
      actctgccg atttgcttg gcggttaca aaccggttg 
      atttgaaat ttgtaatca ccccaattc gtaaagaat 
      tcgaagcac ttttctgtg agtttttcc aagtcgagc 
      caaaccgta cgtttctgg gacacatcg ctcgctgtt 
      acaacgaac agaggtggt cagacctcg ttaaattaa 
      tgacgaaaa gttacgatg cggctattt cgcgtctga 
      agcaaccct cacctaaaa aacacctcc tcgcccgtt 
      ggcaatccc ttggcgaga agttgcaca aactcactt 
      tgcagctga aacagaaaa gtgccctcg cgttcgctt 
      tcattgaag gcagtttag tgagagccg ttgaagagg 
      ggcactggg tccttttgg acgagatta atctagcga 
      acgccgaaa cgctcgaat gtttgtcag gacttttgg 
      agggagcgg aaggctcgc tgtgtttgc tcgaaaggg 
      gcgataaag ccccggtgg aacggcacc ccaatttta 
      ccctctttg cgtgcatga acccttcga cggacgtgg 
      gcaagaagg atcttccag cggggctgc ggaaccgtt 
      ttacagaat tttacgttg atgagttgg tcgagagaa 
      acgacttgg ttttgctgg ttgggagtt atctggaag 
      cgttgtctt tgaaggagg gcgagttgg agcgaattg 
      ttgagtttt atttgaggg tgcggaagg agacggagg 
      tttcgttgg tggatgggt tggggcaca agccgcatt 
      tctcgctga ggtcgcttt gtcgcgcct tgatgatta 
      cagggagga atccgtgtg ggatggtca agaagagtt 
      tgtatgagg cgttttgtt tgagttttt tgacgcagt 
      tggatgagc agtcgtatg ggattgttg agaagttga 
      ttgcgaagt atttggttg gagatgata aggcgttga 
      aagcagttt tgaagcagc ctattccta cgcctaaaa 
      gtaattctg atgattatg tccagtttg agggttatt 
      gggttcgga aaggggatt tggagtgtt tggttcctg 
      atgattacg ttttgaccg agtctgtgc gaaaaaatc 
      ttaaagatt tggtgcgaa ttgtgtcga ttgggggac 
      tgccagttt tactacagg gcgacactt ccgtcggta 
      aaaccagtc tgatcgctt acttggcca agtcaagcg 
      gtaacaaat gcgtccgca taaacaacc acgaacaca 
      ccgatctcc aggaataca ttggctcgt acgtcgccg 
      actgcgagg gcaaactgg tcttccggg aaggcctcc 
      tggtggagg caatgcgca aaggccatt ggatcatcc 
      tcgacgagc tcaacctgg cacctagtg acgtcctgg 
      aggccctaa accgcgtcc tggatgaca atcgcgaat 
      tattcattc cagaaacac aagaaaccg tcaaagccg 
      accccaatt tcatgctct tcgccacac agaatccgc 
      ccggttcgt acggcggac gcaaaatgc tgtcgaggg 
      ctttcagga atcgattcg ttgaactgc atttcaacg 
      aaattccgc cgaaggagt tggagttta ttttgcata 
      agcggtgtc aaatggcac cgtcttatg ccaagaaga 
      tgattaatg tcatgacgg atctacaga ctcggagac 
      ggggttcgg ctgcgtttg ccggaaagc agggtttta 
      tcactttga gggatttgt tcagatggg gcgaaaggt 
      atcgcctgg cccaaaaca cccaaaaat tgtacgatt 
      gggaccaac atttggcgg atgaaggct atttagtcc 
      tagcgggaa gggtcagga aaagtgacg agaaaaaag 
      agattatca ccgttttgc aaaagcacc ttaaacgcg 
      aagttaacc cgcacaatt tattcactc tgtcggaaa 
      aaacatcag ttgtgaccc gagatattt taacaaaaa 
      tttaccaaa atcgcgaca aatacaaaa acattgtgt 
      ggacctata atatgagac agttggcgg ttttggtgg 
      ctaaagcgt tccatttca aagaacctg ttctcctag 
      tgggtgaaa caggaggtg gcaaaacaa ccgtttgta 
      aattaattg ctgaattga atcagcagg aattaattt 
      cggttaact gtcacatgc ataccgaaa gcagcgatt 
      ttattggtg gtttaagac cggtcagag atcatgtgg 
      acgatgatg ttaacaaat tatttgaat gggttgacg 
      gtcctttaa ttaaagcca tgttaaaag gcaacgttt 
      ttctcgccg acgaaattt cgctggctg atgatagtg 
      tgctcgaac gtcttaact ctcttctag agccggaac 
      ggactttat tattggctg aaaaagggg ccgatatca 
      ataatccaa acaactcgg aattaattg tcgccgatc 
      ccaactttt attttattg ggacgatga atcctggag 
      gtgattttg gcaagaaag aattgtcac cagctttgc 
      gaaatcggt ttacggaga tctggtgtg aatcgtgca 
      ctgatcgcg atgatttga ttgcgataa ttgaacaaa 
      atgtcaagg agggaattt cttttggga atcaacagg 
      atggatcga gtgggattg gtaaaaata ttatggatt 
      ttgtggaat ggtttcgag gtactgaaa ttgggaaaa 
      ggtgcactg taagtattc gcgacattt taacttggg 
      taaacttca tcaacaact gtgctgaaa aaatcggag 
      tttacgaat cgtaccttc acggcttac atctcacat 
      ttctggacg ctttaggca ccggaatca ccggaacag 
      aaaatttaa acaacttga aaaaattca aacactgtt 
      gcgaactgt tcattaaaa aacaactaa attcttcaa 
      ccctcccgc acgtatttt cgccaaatt tactgatcg 
      aagaatcgg acaattcat tcggaatta aacccttct 
      acatcgaaa cgggtaatg agcctccca ctcccaact 
      ccttcacat tcagcgctc ccacaacga catacaaca 
      ctctaagat tattgagag gaatgcagt tgaacaagg 
      cgattttac ttgagggca gtcccggag tcggcaaaa 
      ctagccttg ttacagctt tggcaaaac taacccgcc 
      ataaaatct tccgtgtga atttatccg accaaaccg 
      atatttccg acctgtttg gggccgatt taccggtgg 
      aggggggct caggggggc agttctcgt ggcgtgatg 
      gccccctgc ttcaggccc ttaaacaag ggcactgga 
      ttttactcg acgagttaa atttggcct cacagtcgg 
      ttctggaag gtttaaatg cgtgtttgg accaccgag 
      gcgagattt ttatccccg agttgggca aaacgttcc 
      acgtaagac caggaacgc gttttttcg cctgtcaga 
      accccctta aacaagggg gcagccgga ggggtttgc 
      cccagtcgt ttctcaatc gttttatcc aagtttacg 
      tcaactcgc tcacttcta aagacttgg aattaattt 
      tgactaacc aattccggg ccattcctc gtgaaataa 
      tcgacaaga tgttgaaat ttaactcaa gggtttgcg 
      atgagttgg atggtcata gttttggga ataaaggcg 
      cgccttggg agttgaact tacgcgatt tgagtcgat 
      ggtgtgagg ccatgattt atcactttg aagctaacc 
      caagtgtcg ataagaaat atcaaccgg agagtttgg 
      tgcacttaa tttacgggg atcgcatga ggactttgc 
      gggataaga ggaaagtga gtgagatgt ttgaggaag 
      tgttcggcc ggggcattt ctggtgatg cgcccgttt 
      tgtatacaa atagtgaac aagtgtgga ttggtgatg 
      tggggttgt ggaggaata gggacggcg ttgatgtta 
      atgtgatca gtgacgaga aaagttgtt tggttttga 
      ggacgcagt tgccggttt tgaggagtt tggcctatt 
      gtgttaatc tcaactgga tgagtttac tggtgggga 
      gttctgcgg caggaaaaa gcacagtgg tgcgagctt 
      tggctcaca tcgttggaa aaacgctcc gaactttgc 
      ctgtaacga gtgcaatgg atacaacgg atatcttgg 
      gaggattcg aacaggtaa gtcaagttt tgctctgat 
      tgttgacaa gatttattt ctttatgac gtagtaata 
      catacagaa aaaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY