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Gene information
General information
TC001037 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10026560..10029022
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001037
    iBeetle screen iB_06688

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    tropharium size decreased - Number of animals showing the phenotype: 4
    vitellarium size decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber position anteriorized - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentially fused - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001037 RA
      MTEYKLVVV GAGGVGKSA LTIQLIQNH 
FVDEYDPTI EDSYRKQVV IDGETCLLD 
ILDTAGQEE YSAMRDQYM RTGEGFLLV 
FAVNSAKSF EDIGTYREQ IKRVKDAEV 
VPMVLVGNK CDLTSWAVD MNQAREVAR 
QYGIPFVET SAKTRMGVD EAFYTLVRE 
IRKDKTRSG KRQHRYIGG HGRVPFKLR 
CTIL
    CDS sequence
    • >TC001037 RA
      atgactgaa tacaaacta gtagtagtt ggagcaggt 
ggtgtcggc aaatcagct ttgaccata caattaatc 
caaaatcac ttcgtcgac gaatacgac cctaccatt 
gaagactcc tatcgaaaa caagtagtc atcgatggg 
gaaacgtgt ttactggat attttggat acggcagga 
caggaagaa tacagtgcc atgcgagac cagtacatg 
aggacaggg gaaggtttc cttttggtt ttcgccgtt 
aattcagct aaaagtttc gaagacatt ggaacatac 
agggaacaa attaaaagg gttaaagat gccgaagtc 
gtaccaatg gtactcgta ggaaacaaa tgcgacctc 
acttcgtgg gctgtagac atgaaccaa gccagagag 
gtggcgcgg cagtacggg atcccgttc gtggagacg 
tcggcgaag accaggatg ggtgtggac gaggcattt 
tacacgtta gttagagaa atacgtaag gacaaaacg 
agaagcggt aaacgccag cacaggtac atcggcggc 
cacggtcgt gttcctttt aagttgcga tgcaccatc 
ctttaa
    mRNA sequence
    • >TC001037 RA
      tggggtaca aaagagtgg ttgacgttg tttacgtct 
gtggtgatg ctatgatgg gtgggttgt gaagctgaa 
ggtgtgttg gcagcattg tttagtagt gcatttgac 
ggtcttgat aacttttgt tactctttc gttcttgat 
tacaattaa ttagagtaa tttactggg catacgagt 
gttcttaat tgcttcagt ttaaatgtg tgctgtgtt 
gattttatt atgctggga taacgaata agaagacct 
gcatagcct gcaaaaatt aataaaaaa aattaaaaa 
ccgtgtgct tcttggtat tttcgtttg cttagccgt 
tttggtatt ttctacata aaatgactg aatacaaac 
tagtagtag ttggagcag gtggtgtcg gcaaatcag 
ctttgacca tacaattaa tccaaaatc acttcgtcg 
acgaatacg accctacca ttgaagact cctatcgaa 
aacaagtag tcatcgatg gggaaacgt gtttactgg 
atattttgg atacggcag gacaggaag aatacagtg 
ccatgcgag accagtaca tgaggacag gggaaggtt 
tccttttgg ttttcgccg ttaattcag ctaaaagtt 
tcgaagaca ttggaacat acagggaac aaattaaaa 
gggttaaag atgccgaag tcgtaccaa tggtactcg 
taggaaaca aatgcgacc tcacttcgt gggctgtag 
acatgaacc aagccagag aggtggcgc ggcagtacg 
ggatcccgt tcgtggaga cgtcggcga agaccagga 
tgggtgtgg acgaggcat tttacacgt tagttagag 
aaatacgta aggacaaaa cgagaagcg gtaaacgcc 
agcacaggt acatcggcg gccacggtc gtgttcctt 
ttaagttgc gatgcacca tcctttaag acagactca 
tcgtttcga tgaccagca aaaaaattt aaaaaaatg 
ggatcgtgt tttgcttcg catttttct cctctgtga 
aagtgtgat agtgtaaaa agtaatagg ggggagcga 
cgcttcact cttctcccc cgattgtta tatcatttg 
acaattcgt gtgcgtgtt tccataatt ccagtgtgc 
gaaaatgtg taagaggaa gcttatgaa agaagtttt 
tttatgtac cacgttttg cttttagtt caagtgtgt 
cttttctgc tcagtatat tcttacttg tcgattctt 
aaatttgct gtaaatact ttttagata ttatattga 
aaaagcttc ggaaacatt tttcgtctc tttgcacaa 
atttttgtt ttaaagagg ataaataca cggttttat 
atatgtcag atattaaaa ttttatatg tatattctg 
aatgtgatg tatatttta ttgatagtt ttatttagt 
tctgacgag aaatttgta cagttgcgc aacattttt 
ggcgagaaa agttgatat taagattat tgtttctaa 
cggatttcc ttttaatta tgtaatatc tcaggaata 
gcatatcat gtatagttt gcgcacaaa tttgtatgt 
gcctcaaag agaactcgt tctcttcgt tttttgttg 
atattaatg tagttatcc tctttaatg agaattttt 
tgtcatgtt tctggatac gatgaataa actaatcgc 
tcatctgcc tcccaattg aaccagttc gttttgttt 
tcgttttat tcctcgtat tctggcata aagtatagt 
taacttcaa gcaatgatc aatcgttaa agaaatata 
accaagttt aagcttaat cttggtctt gacatgtta 
ttccccgtt ttttcatat gagattttt cttaatgtt 
ctactaatc tagttttaa ttttgcctt catatatta 
tacaaaaag aagaaaaaa caaaataaa aatatatgt 
agcaattta gtttataga tatgtctac taaaattct 
cttattaat aaaatgtta aatattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY